Coastal aquifers are increasingly vulnerable to saltwater intrusion (SWI) due to the combined effects of sea-level rise, land subsidence and groundwater exploitation. However, in many low-lying coastal plains, the spatial distribution and dynamics of salinization cannot be fully explained by these drivers alone. This study presents a process-based synthesis of the Volturno Coastal Plain (VCP) (southern Italy), integrating geological, geomorphological and hydrogeological evidence to identify the main controls on SWI.The results highlight that present-day salinization is strongly controlled by the inherited Quaternary stratigraphic architecture, which modulates the effects of external drivers. In particular, the MIS 5 transgression defines the maximum inland extent of marine influence, while the Holocene evolution controls the internal organisation of the coastal aquifer through the development of lagoonal environments and the infilling of the Last Glacial Maximum (LGM) paleovalley. This heterogeneous depositional framework exerts a first-order control on groundwater flow pathways and salinity distribution.Within this inherited structure, land subsidence and relative sea-level rise act as co-dominant processes that amplify system vulnerability by lowering hydraulic gradients and facilitating inland salinity migration. Groundwater exploitation and climatic variability further modulate these dynamics by introducing strong temporal variability, without altering the fundamental spatial controls.The integration of hydrochemical, geophysical and geomorphological data demonstrates that salinization is not uniformly distributed, but preferentially develops in sectors where stratigraphic heterogeneity, subsidence and low hydraulic gradients converge. These findings challenge simplified interpretations based solely on external forcing and emphasise the importance of internal aquifer structure.The conceptual framework proposed here provides a transferable approach for interpreting SWI in other Mediterranean coastal systems and highlights the need for integrated, process-based assessments of coastal vulnerability under future environmental change.

The present study focused on characterising the sperm motility pattern in Mytilus galloprovincialis, among the most economically and ecologically important bivalve molluscs in European coastal areas, as a key step in developing sperm management protocols. To this aim, computer-assessed sperm motility parameters were evaluated as a function of time after activation and post-collection storage. In addition, the effect of combined low osmolality and pH on sperm motility was evaluated for the formulation of diluents capable of inhibiting sperm motility. Sperm motility parameters remained completely unchanged up to 60-80 min after activation, also when semen was activated after 6 h of cold incubation. All hypoosmotic solutions tested (100 to 600 mOsm) inhibited sperm motility, which could only be restored in the 600-mOsm inhibited samples, irrespective of the pH of the diluent. The ease of handling and long-lasting motility properties demonstrated by M. galloprovincialis spermatozoa may facilitate the application of technologies used in both hatcheries and laboratory research. In addition, the simply formulated diluent DI600 (2% NaCl, pH 7.7) can be considered a promising medium for the formulation of cryoprotective extenders or diluents for post-collection semen management.

The standardization of procedures for the post-collection management of semen samples is a prerequisite for the development of husbandry techniques, as well as for the use of semen in laboratory research. Despite the relevance of the sea urchin Sphaerechinus granularis as a laboratory model, the basics related to sperm collection and storage have received little attention. The aim of this research was to optimize procedures for the management of S. granularis semen, focusing on the post-collection storage of samples. Specifically, the effects of post-collection storage conditions on sperm motility parameters were evaluated; motility was assessed by computer-assisted analysis (CASA-mot) upon activation (t0), and 15 min, 30 min and 60 min after dilution in freshly collected semen, and in chilled and cryopreserved samples. Motility parameters remained unchanged for up to 60 min after activation in both fresh and chilled semen. In cryopreserved samples, sperm motility upon activation was significantly lower than in fresh semen; however, no differences with respect to t0 values were recorded when thawed semen was cold- incubated for up to 60 min post activation, thus meeting standards required by germplasm cryobanks. Post-activation longevity showed by both fresh and stored S. granularis semen, as well as its resistance to cryopreservation, are positive spermatological features that will allow the running of easy management procedures, encouraging a wider use of S. granularis in hatchery practices, as well as in laboratory activities.

The aim of the present work is to develop a laboratory-scaled methodology for an on-demand supply of semen from the sea urchin Paracentrotus lividus. Firstly, sea urchin specimens were acclimatized to the long-term rearing in a recirculating aquaculture system and gonad maturation was obtained under controlled conditions. Semen samples were then collected from mature sea urchins and cryopreserved. Finally, post-thawing motility was evaluated, to verify whether the cryopreserved semen had maintained enough viability to be used in laboratory activities. The post-thawing motility parameters remained quite unchanged for up to 60 min after activation; moreover, the semen even retained the ability of motility activation for 60 min after thawing. This motility pattern makes the use of cryopreserved semen a feasible option in spermiotoxicity bioassays. The preliminary ecotoxicity test, carried out using motility parameters as endpoints, showed sensitivity levels to cadmium falling in the same order of magnitude as those recorded for fresh sea urchin semen and for cryopreserved sea bream semen. . Therefore, semen samples produced and stored according to the developed methodology described in this paper, can be considered a promising and sustainable alternative to those collected from mature sea urchins harvested in the field.

Due to the continuous development, production and consumption of nanoparticles (NPs), their release, fate and effects in marine coastal environment can represent a major concern. The aim of this study was to evaluate the toxicity of ZnO nanoparticles (ZnO NPs) and compare it to bulk ZnSO4 on three macroinvertebrates: the isopod Cymodoce truncata (i.e. used for the first time in ecotoxicology), the amphipod Gammarus aequicauda and the sea urchin Paracentrotus lividus. This study showed concentration- and time-dependent relationships for all biological models for both ZnO NPs and ZnSO4. Both Zn forms elicited high toxicity to G. aequicauda and C. truncata juveniles, but ZnO NPs induced comparable responses to both species (96h-LC50 = 0.30 and 0.37 mg/L for G. aequicauda and C. truncata, respectively; p > 0.05), while differences were found after ZnSO4 exposure (96h-LC50 = 0.28 and 0.63 mg/L, respectively; p < 0.05). ZnO NPs generated sub-lethal effects on P. lividus embryos (72h-EC50 = 0.04 (0.03, 0.05) mg/L), not significantly different from ZnSO4 ones (72h-EC50 = 0.06 (0.05, 0.07) mg/L). Effects of ZnO NPs were similar to existing literature data for other testing species. C. truncata can be considered as a promising new biological model in (nano)ecotoxicology.

The implementation of gamete cryobanking in aquaculture may improve hatchery practices such as assisted reproduction and genetic selection of desired strains. This paper preliminarily investigated the technical feasibility of cryobanking semen of the common pandora Pagellus erythrinus, an important fishery resource in the Mediterranean area and a promising species for the diversification of productions. Post-thawing motility was evaluated in common pandora semen samples cryopreserved according to eight previously selected protocols and stored in liquid nitrogen for eight years. In order to mimic the practical use of long-term cryostored semen samples, the proportion of living sperm in which motility could be activated was evaluated, by computer-assisted analysis, on thawing and after 3 h of post-thawing incubation at 18 degrees C as biomarker of sperm viability. The assessed parameters were percentage of motile spermatozoa (TM), curvilinear velocity (VCL), lateral head displacement (ALH), beat-cross frequency (BCF) and dance (DNC). The cryostored sperm of the common pandora proved to have promising features. In fact, percentages of motile sperm higher than 40% were always obtained on thawing; moreover, the semen retained its activation ability in the times after thawing, showing no or slight alterations in the motility profile also when activated after 3 h of post-thawing incubation. The best post-thawing performances were recorded with the EG5%-based extender under the F1 curve (equilibration at 2 degrees C; freezing from +2 degrees C to -70 degrees C at 3.8 degrees C/min), that showed the highest percentages of motile cells (80 +/- 3) and no significant alterations in any of the evaluated motility parameters, followed by the EG10% based extender under the F2 curve (equilibration/cooling from 20 degrees C to 2 degrees C at 1 degrees C/min; freezing from +2 degrees C to -150 degrees C at 23.3 degrees C/min). Such a post-thawing resistance of long-term cryostored samples, encourages further studies for the inclusion of the semen of the common pandora in cryobanks aimed at use in reproductive biotechnologies for aquaculture applications and as a model in laboratory research. Therefore, the present paper contributes to the implementation of semen cryobanking as a technological tool in support to aquaculture, using as a model a species of potential aquaculture interest. In addition, the creation of germplasm cryobanks for aquatic species comply with the EU ARRIVE (Animal Research: Reporting of in Vivo Experiments) guidelines that promotes a coordinate use and sharing of biological samples from animal models in laboratory research.

The coastal lagoon of Lesina, is situated on the Italian southern Adriatic coast, in a primarily rural area. Agriculture is the primary economic activity, while the lagoon supports only small scale fisheries and one aquafarm enterprise. However, the lagoon ecological state shows a slow but constant deterioration. In this study, we a) assess the causes for this decline; b) evaluate why the existing regulations have failed to protect the environment from this deterioration; c) explore the potential for community involvement in the management of the lagoon; d) formulate suggestions for developing a management plan that will ensure the preservation of the lagoon and the well-being of the community, according to a shared stakeholders' vision. For this purpose, available social, economic and physical data were gathered, organized and successively used to identify the causes for the lagoon deterioration. We found that the main causes are: increased discharge of sediments and nutrients from inland activities; unmanaged fishing; loss of human capital; unclear regulations due to vertical and horizontal lack of coordination among authorities. Further, three workshops were conducted with local stakeholders to identify the multiple problems affecting the lagoon area and discuss possible shared solutions. The workshops highlighted that participants perceived the lagoon as an unexploited source of income, mostly in terms of nature based tourism, and suggested that negative externalities caused by the inland activities and mismanagement of the fishery sector should be accounted for by the authorities. However, the mapping of stakeholders indicates that the tourist sector in the area is not developed enough to effectively promote in a bottom-up fashion the development of a management plan of the lagoon area, which could encourage sustainable tourism by protecting the lagoon and generating economic development for the area.

In this study, we tested the effect of two agar-based biocomposites (differing for the fish meal source), on the Paracentrotus lividus gonad cycle progression and biochemical composition, in an offshore pilot scale trial. The purpose of this study was to identify a feeding regime for P. lividus that would allow adequate gonadal growth without accelerating the gamete maturation, in order to extend the market period. The purpose was pursued through the use of agar as a binding agent in the manufacturing of biocomposites to be used as feed for P. lividus and the realization of special offshore cages. The gonad index, histology, biochemical composition of fatty acids (FA) and free amino acids (FAA) and volatile molecule fingerprint were evaluated. Both the gonad index and the histological analysis showed a slower gametogenesis progression in the gonads of caged sea urchins with respect to the field collected ones, consistent with our previous results obtained testing a similar feed composition in a recirculating system. The amount and the relative proportions of FA and FAA in the gonads of wild and cultured gonads were similar, while the analysis of the volatile substances of the gonads of the sea urchin conducted using the electronic nose, shows that they vary as a result of the different feeding treatments. These results show that P. lividus sea urchins can be housed, under this feeding regime during the recovery phase of the gonads, without acceleration of the maturation of the gametes and thus in conditions to allow the extension of the market period. Moreover, the here tested agar-based biocomposites make it possible to reduce the management costs of the rearing system as they need to be replaced only once a week, and its environmental impact as they guarantee a limited nutrient dispersion in the water.

In this paper, a high-frequency (HF) coastal radar network is described, which is established and maintained by the Institute of Marine Sciences (ISMAR) of the National Research Council of Italy (CNR) for the measurement of surface current velocities in the Gulf of Manfredonia, located in the semienclosed Adriatic Sea (Mediterranean Sea), during 2013–2015. The network consisted of four HF radars (HFRs) that provided hourly sea surface velocity data in real-time mode in a netCDF format compliant to the Climate and Forecast Metadata Conventions CF-1.6 and the INSPIRE directive. The hourly netCDF files are disseminated via a Thematic Real-time Environmental Distributed Data Services catalog supporting OGC compliant distributions and protocols for data visualization, metadata interrogation, and data download. HFR velocity data were compared with in situ velocity measurements by Global Positioning System tracked surface drifters deployed within the radar footprint. The results show a good agreement, with the root mean square (rms) of the difference between radial velocities from HFR and drifters ranging between $20\%$ and $50\%$ of the drifter velocity rms. The HFR data have also been compared with subsurface velocity profiles from an upward looking acoustic Doppler current profiler (ADCP) during winter 2015, to gain information on the correlation between surface and water column velocities. This information is especially relevant for fishery and coastal management applications, where transport of larvae, sediments, and pollutants in the water column are considered. Results show that, at least in the considered period, the velocity in the water column is well correlated, and there is a good agreement between surface HFR and ADCP data (correlations between 0.95 and 0.75). The Gulf of Manfredonia network has been instrumental to the set up of a core of quality control practices and interoperable data and metadata formats that have been subsequently adopted within the Italian RITMARE network and that are presently discussed and refined at the European level through the projects Jerico-NEXT and INCREASE.

The development of sustainable methods for sea urchin juvenile production is currently constrained by high mortality rates during larval growth and the high costs of larval rearing systems management. With the aim of developing a method for the production of juveniles of the purple sea urchin Paracentrotus lividus in a medium-scale recirculating system, the present study focused on the effects of high stocking densities on larval growth. Plutei larvae were reared at three different densities (up to 7 ind/ml) in a semi-static culture system. Larval survival and metamorphosis success were evaluated in order to identify the most effective density range. The highest metamorphosis rates (80%-95%) were obtained at 4 and 7 larvae/ml. These results are comparable with (and in some cases higher than) those reported for the same species at much lower larval densities. In conclusion, the rearing conditions tested here show for the first time that a significantly higher (4 ind/ml) stocking density than those of traditional P. lividus rearing methods (based on large volumes and low densities) can be adopted, thus supporting the feasibility of an increase in the final output of competent larvae with no increase in rearing volumes.

Drugs such as oral contraceptives and hormone replacement therapies are known to find their way into rivers, lakes and seas, and have the potential to affect reproduction and development of the wildlife. The knowledge of the reproductive mechanisms and their regulation in aquatic species is of fundamental importance for predicting and preventing the damage by the increasing release of such chemicals in the environment. Mifepristone, a synthetic steroid used as a drug for chemical abortion, works by blocking the effects of progesterone. Its presence in fresh and salt water has been reported, representing a danger for aquatic species. In this frame, we evaluated in both acute and chronic exposures, the effects of mifepristone on the reproductive performance of the sea urchin P. lividus. In both acute and chronic exposures, mifepristone did not affect the histological structure of the gonads. However, mifepristone administered to females caused the decrease of the percentage of normal developed plutei larvae compared with the control, whereas it did not alter sperm motility parameters and fertilization success in males. The immunohistological localization of progesterone receptor-like immunoreactivity on the plasma membrane of oocytes and ova and the molecular weight of a progesterone receptor-like immunoband identified by western blotting, are in agreement with a membrane progesterone receptor deducted from the genome sequence of the sea urchin Strongylocentrotus purpuratus and suggest that in P. lividus mifepristone actions may be mediated by a progesterone receptor.

Shellfish culture, based on ecological and market assessment, is considered a driving force for socio-economic change in ecologically complex coastal systems such as lagoons throughout the Mediterranean area. To diversify fish production, the Pacific oyster Crassostrea gigas was cultured at commercial farms in the Varano Lagoon (SE Italy). The aims of this study were to evaluate through four condition indices (CI, CICG, CIE and AFNOR index), the Polydora index (PI), lipid content, quality and market aspects of oysters reared at two different sites (FO and LA) of the Varano Lagoon, which are characterized by different hydrodynamic conditions. The results of this study highlighted the potential economic benefits associated with sustainable aquaculture development in the Varano Lagoon, proving that the area surrounding the LA site was more suitable for oyster culture, reaching commercial size (60 mm) in a shorter time (6–8 months). Higher growth performance of oysters was observed in the spring, when the nutrient availability positively affected the feeding response of suspension feeders. The opposite was found in winter and in summer, when the decrease in growth could be due to the reduction of nutrient and to the increase of salinity, TSM and ISM. The presence of the mud blister worm (Polydora sp.) during rearing could be a real problem, as it damages oyster market value and threatens serious financial loss to the local farmers.

Vessels, specifically ballast water and hull fouling, are a major vector for the introduction of non-indigenous species (NIS) in European seas. The Mediterranean is one of the world's marine regions where their invasion is heaviest. The shallow Adriatic basin is a highly sensitive area that is already experiencing its consequences. The secondary spread of NIS over a wider area through natural dispersion is a complex process that depends on a wide range of oceanographic factors. This work analysed the dataset of the BALMAS project, in whose framework twelve ports in the Adriatic Sea were subjected to a Port Baseline Survey (PBS), to estimate the natural spread of NIS organisms from their port of arrival to the wider Adriatic basin. Its findings indicate that the prevailing water circulation patterns facilitate the natural dispersal of harmful aquatic organisms and pathogens (HAOP).

Coastal lagoons are highly vulnerable to climate change-related pressures, such as floods and increasing temperatures, which lead to higher oxygen consumption, anaerobic metabolism and dystrophic events. Although these factors have a significant impact on the carbon cycle, the dynamics of dissolved organic matter (DOM) in these systems have not been extensively investigated. DOM can be analytically determined from the concentration of dissolved organic carbon (DOC) and/or from the spectral properties of chromophoric dissolved organic matter (CDOM), which is the light-absorbing fraction of DOM. In the present study, we investigated the spatio-temporal distribution of surface water trophic variables (Chl a and DOC) and CDOM in two Mediterranean lagoon systems, the Oristano Lagoon-Gulf system (OLG) and the Varano Lagoon (VL), in order to provide quantitative information on the dynamics of DOM in these systems. Furthermore, we assessed the value of CDOM-related indices (i.e. absorption coefficients, spectral slopes and Specific UV Absorbance at 254 nm [SUVA254]) as tools for describing the dynamics of DOM in coastal lagoons, irrespective of geographical settings, environmental conditions and anthropogenic pressures. In OLG, spatial heterogeneity and compartmentalization, with salinity varying from <1 (riverine sites) to >50 (Mistras Lagoon), affected the distribution of DOC and CDOM, with the lowest values on the south side and at sites far from riverine input. In OLG, the highest DOC and CDOM values were found in the sediment pore-water of the organic-rich Cabras Lagoon, where they were nearly double those of the water column. In VL, salinity was homogeneously distributed throughout the lagoon, which indicated a mixing of freshwater with marine waters. DOC and CDOM values were on average lower in VL than in OLG. However, in VL, DOC and CDOM showed strong peaks following a flood (September 2014) and a dystrophic event (July 2015), demonstrating the quick response of the system to environmental perturbation. In OLG, absorption coefficients at 280 nm and 350 nm were slightly negatively correlated with salinity, which indicated the influence of terrigenous inputs at riverine sites. In contrast, in VL, CDOM varied linearly and positively with salinity as a result of the in situ input of organic matter from phytoplankton during the dry season. Segment analysis showed that besides the differences between the two investigated systems, the trophic variables and optical parameters analyzed in the present study shared a common relationship. These results suggest that CDOM indices can be good predictors for the estimation of DOM. Overall, the present study provides insight into the dynamics of DOC and CDOM in little-studied Mediterranean lagoons and demonstrates that the CDOM indices can be a valuable, cost-effective and simple tool for describing the trophic conditions of these systems.

Sea urchin larvae have developed metamorphosis and settlement stages as an adaptation serving to increase their distribution and find more suitable habitats; for aquaculture farmers, these stages represent a bottleneck, as the cost-effective production of large numbers of metamorphosed larvae in confined conditions remains a major constraint. The aim of this study was the development of a reliable metamorphosis cue for Paracentrotus lividus larvae that is widely available and easy to prepare and use even at the hatchery scale. For this purpose, substrates of environmental origin (GUS - ground Ulva spp. solution) and chemical origin (histamine) were tested for their effectiveness, evaluating the metamorphosis rates of competent P. lividus larvae after 24-h exposure. In addition, differences in metamorphosis rates following exposure to scaled concentrations of both fresh and frozen-thawed GUS were evaluated, with a view to the latter's routine adoption as a metamorphic cue. The metamorphosis rates obtained in the presence of GUS were always around 80%, using both fresh and thawed solutions. Decreasing GUS concentrations resulted in significantly lower percentages of metamorphosed larvae. No metamorphosis occurred after exposure to histamine solutions. GUS proved to be an effective metamorphic cue and to have several other strengths. Indeed, Ulva spp. are widely available and easy to collect in the field as well as being currently cultivated, in some cases in integrated multi-trophic systems. Ulva spp. represent a common food source for natural populations of juvenile and adult P. lividus, and GUS can therefore be used in the formulation of a settlement substrate that also serves as food in the initial post-metamorphosis period. Finally, GUS is prepared by grinding fragments of Ulva spp. with FSW in a mortar and then filtering the solution, with no need for time-consuming or expensive methods. It can thus be easily produced on a commercial scale and then stored or delivered after being frozen. In conclusion, the formulation of GUS represents a promising advance in the development of protocols for echiniculture, meeting the need for tailored metamorphosis and settlement methods that support successful and cost-effective production of sea urchin juveniles. Moreover, it could represent an additional valuable method for obtaining marketable products from harvested Ulva grown in integrated multi-trophic aquaculture systems.

Understanding the role of ocean currents in the recruitment of commercially and ecologically important fish is an important step toward developing sustainable resource management guidelines. To this end, we attempt to elucidate the role of surface ocean transport in supplying recruits of European sardine (Sardina pilchardus) to the Gulf of Manfredonia, a known recruitment area in the Adriatic Sea. Sardine early life history stages (ELHSs) were collected during two cruises to provide observational estimates of their age–size relationship and their passive pelagic larval duration (PPLD). We combine these PPLDs with observations of surface ocean currents to test two hypotheses: (1) ELHSs are transported from remote spawning areas (SAs) by ocean currents to the Gulf of Manfredonia; (2) sardines spawn locally and ELHSs are retained by eddies. A historical surface drifter database is used to test hypothesis 1. Hypothesis 2 is tested by estimating residence times in the Gulf of Manfredonia using surface drifters and virtual particles trajectories that were computed from high-resolution observations of surface currents measured by a high-frequency (HF) radar network. Transport to the Gulf of Manfredonia from remote SAs seems more likely than local spawning and retention given a mismatch between observed PPLDs of 30–50 days and relatively short (<10-day) average residence times. The number and strength of connections between the gulf and remote SAs exhibit a strong dependence on PPLD. For PPLDs of 20 days or less, the gulf is connected to SAs on the western Adriatic coast through transport in the Western Adriatic Current (WAC). SAs on the east coast are more important at longer PPLDs. SAs in the northern and central Adriatic exhibit weak connections at all PPLD ranges considered. These results agree with otolith microstructure analysis, suggesting that the arrival of larvae in the gulf is characterized by repeated pulses from remote SAs. This is the first attempt to describe the processes related to Lagrangian connection to, and retention in, the Gulf of Manfredonia that will be complemented in the future using validated numerical ocean models and biophysical models.

The occurrence of the red swamp crayfish Procambarus clarkii is documented in the surroundings of Lake Varano (Puglia region, SE Italy), testifying to the ongoing diffusion of this invasive crayfish in north-eastern Puglia, an area characterised by an extensive network of natural and artificial watercourses. In addition, the species is recorded for the first time in the Salento Peninsula, in the south-western part of the region. The hydrology of the area is dominated by karstic phenomena, and the ecological consequences of the colonization of hypogean environments by P. clarkii are discussed. These records, in conjunction with a number of recent observations made in Puglia and in other regions of southern Italy including Sicily and Sardinia, indicate that the species is far more widespread in the area than previous studies have suggested.

The effects on coastal lagoons of floods resulting from intense rainfall need to be assessed using a multidisciplinary approach, able to describe in real time the occurrence of changes in sediment quality, anticipating risk situations. In the present study the effectiveness as pre-screening tool of the MOT-test, an innovative bioassay that uses sperm motility as endpoint, has been evaluated; its response has been compared with the sediment chemical characterization, in order to determine the effects of a flood event occurred in the Varano lagoon, a poorly anthropized Mediterranean coastal lagoon. The MOT-test effect percentages ranged from 10% to 30%, fully reflecting data from chemical analyses. Organic pollutants showed concentrations always below the critical levels. The highest metal values were found in the south-eastern area; however, only for few metals (mainly Cd), and in few sites, they reached the limits defined by the sediment quality guidelines; consistently, these sites are located in the area where a higher stressors' impact was expected and the highest MOT-test effect percentages were recorded. In conclusion, even if results exclude the occurrence of serious contamination events as a consequence of the flood, the runoff resulting from the intense rainfall event has caused a pollutant load in the lagoon, consistent with the localization of the main anthropogenic activities in the catchment area. Moreover, preliminary data indicate the validity of the approach adopted for the post-flood quality assessment, proving the usefulness of the MOT-test as early-screening tool in the case of extreme events.

An inventory of phytoplankton diversity in 12 Adriatic ports was performed with the port baseline survey. Particular emphasis was put on the detection of harmful aquatic organisms and pathogens (HAOP) because of their negative impact on ecosystem, human health, and the economy. Phytoplanktonic HAOP are identified as species, either native or non-indigenous (NIS), which can trigger harmful algal blooms (HAB). A list of 691 taxa was prepared, and among them 52 were classified as HAB and five as NIS. Records of toxigenic NIS (Pseudo-nitzschia multistriata, Ostreopsis species including O. cf. ovata) indicate that the intrusion of non-native invasive phytoplankton species has already occurred in some Adriatic ports. The seasonal occurrence and abundance of HAOP offers a solid baseline for a monitoring design in ports in order to prevent ballast water uptake and possible expansion of HAOP outside their native region.