Hyper-adrenal activity and increased glucocorticoid hormone release are associated with disruptions in reproductive function and adverse effects on the ovary. The aim of this investigation was to determine whether elevated glucocorticoid hormone levels can influence ovarian IGF-I synthesis and action in vivo. To elevate endogenous glucocorticoid levels, gilts were treated with ACTH during the luteal phase of the oestrous cycle (days 9-13) while the control group received saline. The gilts were subsequently ovariectomized on either day 14 or day 18 of the oestrous cycle. Follicular fluid (FF) was collected from individual follicles; IGF-I and steroid hormone concentrations were determined by radioimmunoassay, and IGF-binding protein (IGFBP) expression was assessed by Western ligand blotting. Granulosa cells were also recovered and placed in culture to determine IGF-I, progesterone (P(4)) and oestradiol-17beta (E(2)) production levels. The cells were cultured in serum-free medium for 5 days and supplemented with: (a) media alone, (b) IGF-I, (c) FSH and androstenedione (A(4)), or (d) IGF-I with FSH and A(4). The FF from ACTH-treated gilts was characterized by elevated (P<0.05) cortisol levels on day 14 and lower (P<0.05) E(2) values on both day 14 and day 18. Lower (P<0.05) IGF-I concentrations were also measured in the FF of ACTH-treated gilts collected on day 18. This altered hormone profile in FF was associated with impaired IGF-I and steroid hormone synthesis by granulosa cells. IGF-stimulated P(4) production (P<0.01) by cells recovered from ACTH-treated gilts on day 14 was lower (P<0.05). By day 18, IGF-I, P(4) and E(2) production by cells from the ACTH group were all significantly (P<0. 05) lower. These results demonstrate that increased glucocorticoid concentrations can disrupt subsequent ovarian IGF-I synthesis and IGF action in vivo and can, potentially, impair follicle maturation.

The effect of cortisol on granulosa cell (GC) insulin-like growth factor I (IGF-I) synthesis, and IGF-mediated steroid production was examined at various stages of follicle maturation. Granulosa cells were recovered from gilts on Days 14, 18, and 20 of the estrous cycle, while luteinizing GC were recovered on Day 21, just prior to ovulation. The cells were cultured in serum-free medium with increasing concentrations of cortisol (0, 1, 10, and 100 μg/mL) for 5 d with or without IGF-I stimulation (10 ng/mL). During culture all cells were supplemented with FSH and androstenedione (A4). Cellular IGF-I, progesterone (P4) and estradiol-17β (E2) production was determined by specific radioimmunoassays (RIA), and cell proliferation was assessed. Granulosa cell IGF-I and steroid hormone synthesis increased (P<0.05) with follicle maturation. Direct exposure to high cortisol concentrations, however, altered both IGF-I synthesis and action. Cortisol treatment lowered (P<0.05) IGF-I production by GC recovered on Days 18, 20, and 21. Furthermore, it reduced (P<0.05) IGF- stimulated P4 synthesis at all stages and decreased (P<0.05) IGF-stimulated E2 synthesis by cells recovered on Day 14. In contrast, cortisol enhanced (P<0.05) FSH-stimulated P4 production by GC collected on Days 14 and 18. The opposing effects on FSH and IGF-I action indicate that cortisol did not promote an overall suppressive effect on cell function, nor did it impair cell proliferation. Hence, these results demonstrate that elevated cortisol concentrations can disrupt both IGF-I synthesis and IGF-mediated actions by porcine GC under in vitro conditions, and that specific disruptions are dependent on the stage of follicle maturation.

The objectives of this study were to evaluate ovarian changes in cows with follicular ovarian cysts following treatment with either GnRH or saline. The parameters determined were the intervals from treatment to observation of a CL and from treatment to disappearance of the cyst, and the association between serum concentrations of LK, FSK and the LH/FSH ratio, before and after treatment, with the test intervals. Thirty-nine cows were identified as having follicular cysts. The GnRH treatment induced a significant increase in LH and the LH/FSH ratio. The gonadotropin response was not associated with the intervals from treatment to CL detection or to disappearance of the cyst. Survival curves for the intervals from treatment to CL detection and cyst disappearance indicate that treatment with GnRH or saline did not yield significantly different results for either parameter. The results question the efficacy of treating cystic ovarian disease with GnRH. (C) 1999 by Elsevier Science inc.

The wood bison (Bison bison athabascae) is a threatened Canadian species that has faced extinction twice in the last 100 yr. Development of assisted reproductive technologies could help ensure the long-term propagation and genetic management of this species. The objectives of this study were to refine estrus synchronization techniques and evaluate superovulatory responses after FSH or eCG administration. In Experiment 1, females were fitted with Syncro-mate B (SMB) implants for 9 d and received an injection of either estradiol valerate (E2V; n = 9) or cloprostenol (PGF; n = 9) at implant insertion (Day -9). In Experiment 2, estrus was synchronized with SMB implants and a PGF injection on Day -9, and superovulation was attempted on Day -2 with either 2500 IU eCG (n = 5) or 400 mg Folltropin-V (n = 5). In each experiment, bison were examined daily for estrual behavior. Ultrasonography was used during the luteal phase to detect ovulation and assess ovarian status; feces were analyzed by ELISA for immunoreactive progestogens (P) to study ovarian endocrine responses. In Experiment 1, a closer synchrony of estrus was observed between Days 2 to 4 among the PGF-treated (77.8%) than the E2V-treated (66.7%) females. Corpora lutea (CL) were detected in 55% of E2V- and PGF-treated females. In Experiment 2, neither treatment successfully induced superovulation, with only a single female per treatment producing ≥ 1 CL. In both experiments, progestogen profiles were similar for each treatment (P>0.05).

The adrenal cortex is believed to be implicated in the high incidence of abortion in the Angora goat. Stimulation testing with adrenocorticotrophic hormone (ACTH) was used to assess the adrenal cortical function in 5 Angora does from herds with a history of abortion and 5 non-Angora does. An acute test involving a single intramuscular (i.m.) injection of 0.25 mg of synthetic ACTH was given during anoestrus, at mid-oestrus, on day 90 and on day 120 of gestation. Blood samples were collected from the jugular vein at 30 min intervals for 1 h before and 5 h after injection. Cortisol concentrations rose within 30 min and returned to baseline values within 3.5 h. Cortisol production was lower (p < 0.01) in the pregnant state compared to the non-pregnant state in both groups. Production of cortisol was consistently lower (p < 0.05) in the Angora does compared to the non-Angora does during anoestrus and pregnancy and marginally so at mid-oestrus. A chronic stimulation test involving once daily injections of 0.5 mg of a depot form of ACTH i.m. for 7 days commencing on day 90 of pregnancy was also conducted. Cortisol concentrations rose to reach a peak on the third day of treatment in both groups. The values then declined in the Angora does despite continued ACTH treatment, while those for the non-Angora does exhibited a second peak. During and following this treatment, two non-Angora does delivered live kids (day 95, day 120). Out of 7 Angora pregnancies, one Angora doe aborted two dead fetuses at day 116. No significant difference in the cortisol response in the acute test was detected between the animals that aborted and their respective cohorts, but the two non-Angora does that aborted had significantly lower cortisol concentrations during depot ACTH administration. Progesterone and oestradiol levels did not differ between Angora and non-Angora animals during pregnancy or on the test days. The results suggest that the steroidogenic response of the adrenal cortex to ACTH stimulation is significantly less in Angora does with a history of abortion than it is in non-Angora does and support the view that the Angora goat would make a more limited adrenal cortical response to a stressful occurrence during pregnancy.

Immunoreactive relaxin activity and progesterone concentrations in weekly plasma samples were measured throughout six pregnancies in five Asian elephants (Elephas maximus). One animal aborted, one delivered a stillborn calf at term and the remaining pregnancies concluded normally with living young. Immunoreactive relaxin activity increased by week 20 of pregnancy to reach a mean peak concentrations of >6 ng/ml in the second trimester. This was followed by a slow decline beginning approximately at 30 weeks before term. A smaller, secondary relaxin rise was observed during the final eight weeks preceding parturition and circulating concentrations remained above pre-pregnancy baseline values for 72 h after birth. In contrast, progesterone concentrations increased immediately after mating and rose to a maximum of >2 ng/ml by mid-gestation. As with relaxin, progesterone concentrations began to decline gradually approximately 30 weeks before birth. More frequently collected samples before, during and after parturition revealed a decline to pre-pregnancy levels between 2–5 days before parturition, in contrast to elevated relaxin levels (1 ng/ml) during this time. The aborted pregnancy at week 35 was accompanied by a precipitous decline in both immunoreactive relaxin and progesterone concentrations one week before the visible termination of the pregnancy. The delivery of the term stillborn calf occurred five weeks after both immunoreactive relaxin and progesterone concentrations had declined to pre-pregnancy values. Results suggest that immunoreactive relaxin may be important, along with progesterone, in the maintenance of pregnancy in the Asian elephant as well as playing a role in preparation for birth and possibly to indicate foetal well-being.

The amounts of total protein, albumin, fibronectin, alpha 2-macroglobulin (alpha 2-M), immunoglobulin G, ceruloplasmin and antithrombin were determined in fluids collected from 53 preovulatory equine follicles and compared with the contents of estradiol-17 beta, progesterone and androstenedione, with follicle size and the amounts of the equivalent proteins in normal equine plasma. The concentration of fibronectin and the fibronectin/albumin ratios increased significantly with follicle size and with follicular estradiol levels. The alpha 2-M levels and alpha 2-M/albumin ratios correlated with follicle size but not with hormone content. Both fibronectin and alpha 2-M were present in lower amounts in follicular fluid compared with plasma while the other proteins were present in similar amounts. Among the proteins evaluated, there was a positive correlation between the amount of the protein in the follicular fluid and the molecular weight of the protein.

Two experiments were conducted to evaluate the effects of chromium (Cr) supplementation on early lactation performance of Holstein cows. Experiment 1 involved 12 primiparous (PP) and 22 multiparous (MP) cows, while exp. 2 involved 18 PP and 22 MP cows. In each trial, cows were fed total mixed rations (TMR) and assigned to one of two treatments: control or 0.5 mg kg−1 supplemental chelated Cr from week 6 prepartum to week 16 postpartum The cortisol response to synthetic adrenocorticotropin (ACTH) intramuscular (i.m.) and the luteinizing hormone (LH) response to synthetic gonadotropin-releasing hormone (GnRH) i.m. were evaluated as well, during weeks 2 and 6 postpartum in exp. 2 During the first 16 wk of lactation, supplemental Cr tended to increase milk yields in PP cows by 13% (P = 0.06) and increased milk production by 7% (P < 0.05) in exps. 1 and 2, respectively, but had no effect in MP cows. Despite increased milk yield, Cr supplementation had no adverse effect on milk composition, reproductive performance, or health status. Furthermore, Cr supplementation tended to increase the milk lactose content in PP cows. Supplemental Cr reduced serum β-hydroxybutyrate levels and may reduce subclinical ketosis in MP cows. In addition, in overconditioned, aged cows, Cr supplementation may reduce the incidence of certain other metabolic disorders. Peak values and production rates of cortisol in Cr-supplemented cows tended to be greater (P < 0.10) than in control cows at week 6 Basal LH concentrations did not differ between the two treatments for either week However, the Cr-supplemented cows released less (P < 0.05) LH than did control cows at week 6. Results from hormonestimulation tests suggest that the Cr-supplemented cows may have experienced a greater stress with their increased milk production. However the time in postpartum anestrus and the time to conception were similar, suggesting that Cr supplementation may have mitigated the slower return to fertility usually seen in higher producing PP dairy cows. Further study is needed to determine the effects of Cr supplementation on reproductive performance, health status, and metabolic disorders in dairy cows. Key words: Chromium, milk production, luteinizing hormone, cortisol, reproduction, dairy cows

To assess the possible influence of increased endogenous glucocorticoid levels on ovarian function, gilts were treated with either adrenocorticotrophic hormone (ACTH) or saline during the luteal phase of the oestrous cycle, from day 9 to day 14, and ovariectomised on day 14. Individual follicles were dissected from each ovary and their follicular fluid (FF), granulosa and theca cells were recovered. Cell viability was determined by dye exclusion. Cortisol (C), oestradiol-17 beta (E(2)), oestrone (E(1)), testosterone (T) and androstenedione (A) concentrations were measured in FF while cellular oestrogen and androgen production was assessed during a 3 h incubation period.The steroid hormone profile in FF was significantly altered in the treatment group. Follicles collected from ACTH-treated gilts were characterised by elevated C (P<0.001), and E(1) (P < 0.05), but lower T and A (P < 0.01) levels; E(2) values also tended to be lower, but not significantly so. An increase (P < 0.01) in the proportion of follicles with lower granulosa cell viability was observed on the ovaries of ACTH-treated gilts. There were no significant differences in E(2) or E(1) production, based on viable cell numbers, during the 3 h incubation by either granulosa or theca cells from control and ACTH-treated gilts. Theca cells collected from treated animals, however, produced significantly lower (P<0.01) A concentrations. These results indicate that ACTH treatment leading to elevated endogenous glucocorticoid levels during the luteal phase of the oestrous cycle, prior to follicle recruitment, can reduce the proportion of viable granulosa cells per follicle. This change is associated with altered steroid hormone concentrations in FF, but does not appear to involve suppression of aromatase activity.

Available evidence implicates environmental factors in the pathogenesis of autism spectrum disorders (ASD). However, the role of specific environmental chemicals such as phthalate esters that influence ASD risk remains elusive. This paper systematically reviews published evidences on association between prenatal and/or childhood exposure to phthalate and ASD.Studies pertaining to systematic literature search from Scopus, PubMed, PsycInfo and Web of Science prior to December 2015 were identified. The authors included studies which assessed the effect of exposure to phthalates on occurrence of ASD. This comprehensive bibliographic search identified five independent studies. Each eligible paper was summarized with respect to its methods and results with particular attention to study design and exposure assessment. Because of the heterogeneity in the type of included studies, different methods of assessing exposure to phthalates and the use of different statistics for summarizing the results, meta-analysis could not be used to combine the results of included studies.The results of this systematic review have revealed the limited number of studies conducted and assessed phthalate exposure. Seven studies were regarded as relevant to the objectives of this review. Two of them did not measure phthalate exposure directly and did not result in quantitative results. Out of the five studies in which phthalate exposure was mainly measured by the examining biomarkers in biological samples, two were cohort studies (one with positive results and another one with not clear association). Among the three case control studies, two of them showed a significant relation between exposure to phthalate and ASD and the last case control study had negative results. Indeed, this case control studies showed a compromised phthalate metabolite glucuronidation pathway, as a probable explanation of mechanism of the relation between phthalate exposure and ASD.This review reveals evidence showing a connection between exposure to phthalates and ASD. Nevertheless, further research is needed with appropriate attention to exposure assessment and relevant pre and post-natal cofounders.

Factor XI (F XI) deficiency is an autosomal recessive coagulopathy found in Holstein cattle. Affected animals have a 50% greater prevalence of repeat breeding. Therefore, several parameters describing ovarian function were studied. Daily blood sampling revealed that progesterone concentrations were slower to decline from a peak at day 16 (p < 0.01) to values less than 3 nmol/L in F XI-deficient cows (5.14 +/- 0.69 days (mean +/- SD) versus 4.05 +/- 0.63 days in control animals), resulting in an oestrous cycle length of 24.7 +/- 2.1 days compared to 22.9 +/- 3.0 days, respectively. This was not due to an alteration in the availability of prostaglandin F-2 alpha (PGF(2 alpha)) or oxytocin (OT) involved in luteolysis. No significant differences (p > 0.05) were seen between normal (n = 7) and F XI-deficient (n = 7) cows in the peak values or the area under the curve for the pulse in 13,14-dihydro-15-keto PGF(2 alpha) in response to OT challenge or in the parameters describing the pulse of ovarian OT secretion after PGF(2 alpha) injection (n = 7 for each) between days 12 and 14. Ovulatory follicular development was assessed by ultrasound monitoring and plasma 17 beta-oestradiol values at 8-h intervals after a luteolytic injection of cloprostenol (n = 6 for each). Follicular diameter was smaller (p < 0.05) and accompanied by lower peak oestradiol values near the time of ovulation in F XI-deficient cows. The results suggest that the oestrous cycle in F XI-deficient cows is characterized by a slower process of luteolysis that may be associated with smaller follicular development.

This study was designed to noninvasively characterize estrous cycles and evaluate the effectiveness of estrous cycle synchronization protocols in wood bison. Daily urine and fecal samples and behavioral observations were collected over 2 breeding seasons from a captive herd of 18 adult, female wood bison. All samples were analyzed by enzymeimmunoassay for pregnanediol-glucuronide (PdG: urine) or progesterone (P4: feces). In Year 1, animals were divided into 3 treatment groups: 1) natural estrous controls, n = 6; 2) PG, 2 injections of cloprostenol, 11 d apart, n = 6; 3) SMB, Syncro-Mate-B implants left in place for 9 d with an estradiol valerate injection at the time of implantation, n = 6. In Year 2, control animals remained the same and synchronization regimens were repeated, but with each animal receiving alternate treatments. In both years, 11 d after treatment cessation, females were subjected to ultrasound examination to evaluate ovarian activity. Combined endocrine and behavioral data from natural estrous cycles demonstrated seasonal effects, with mean cycle lengths of 20.8 ± 0.3, 21.5 ± 0.5 and 21.1 ± 0.4 d, based on behaviors, PdG and P4 respectively. Females which demonstrated behavioral estrus did so within 4 (SMB, 1112) or 5 (PG, 1012) days after treatment. The SMB-treated animals exhibited estrous behavior within a 3-d span, with 55% exhibiting estrus 3 d after treatment ended, while the onset of estrus in PG-treated animals occurred over 4 d, with only 40% occurring on a single day. At the second estrus, 77% of SMB-treated animals and 74% of PG-animals demonstrated estrous behavior within 4 d, while at the third estrus, 88 and 90% of treated animals showed estrous behavior within 3 or 4 d, respectively. Ultrasound assessment revealed that only 50% (1224) of treated animals possessed corpora lutea (CL), 67% in PG-treated females and 33% in SMB-animals. The total number of CL tended to be greater in PG- (10) than in SMB-(5) treated animals. Females with no CL demonstrated attenuated progestin profiles compared to those with ovulations. Additionally, a total of 28 follicles was detected, with a greater proportion (P < 0.05) from SMB-treated animals (78.5%) than from those receiving PG (25%). These results indicate that wood bison estrous cycles and synchronization can be monitored using either urine or feces. However, ovulation and normal luteal function did not occur at the first estrus after synchronization, perhaps due to stressors associated with capture and restraint.

An extraction and assay procedure to measure fecal estradiol-17 beta and progestin concentrations in several cat species was developed and validated for use for noninvasive monitoring of ovarian function. Fecal samples were collected over a range of 3-20 months from female tigers (three), lions (three), snow leopards (three), cheetahs (two), caracals (two), and domestic cats (five). Samples were extracted with 90% methanol, lipids removed with petroleum ether, and the estradiol and progestins in the methanol measured by radioimmunoassay (RIA). High Performance Liquid Chromatography (HPLC) fractionation and subsequent RIA of the fractions indicated that the estradiol-17 beta antiserum cross-reacted primarily with estradiol-17 beta in the feces of lions and tigers and was assumed to be specific for estradiol-17 beta in the feces of other species as well. However, there were several immunoreactive compounds, presumably progesterone metabolites, excreted in the feces which varied both quantitatively and qualitatively among species. The behavior of tigers, lions, cheetahs, and caracals was visually monitored during the collection period and frequency of sexual behaviors was positively correlated with increases in fecal estradiol in all species observed. The mean fecal estradiol-17 beta peaks were as follows: tigers, 128.0 +/- 13.1; lions, 186.0 +/- 14.8; snow leopards, 136.7 +/- 15.9; cheetahs, 140.9 +/- 9.0; caracals, 24.5 +/- 4.0; and domestic cats 158.9 +/- 19.3 ng/gm. Fecal progestin concentrations rose significantly (P < 0.001) only after breeding or during pregnancy and were as follows: tigers, 5.6 +/- 0.6; lions, 1.9 +/- 0.1; cheetahs, 8.4 +/- 1.1; and caracals, 2.4 +/- 0.4 mu g/gm. Fecal progestins were elevated for one-half to two-thirds of the gestation length during presumed pseudopregnancy but remained elevated throughout successful pregnancies. These results suggest that ovarian function can be monitored noninvasively in the family Felidae by the measurement of fecal estradiol-17 beta and progestin concentrations (C) 1995 Wiley-Liss, Inc.

Faecal samples collected for variable periods from 12 animals and five species of cats were assayed for progesterone and oestradiol content by application of standard radioimmunoassays to aliquots (50 microliters) of methanol extracts (4 ml) of a mixture of 0.5 g sample, 0.5 ml water and 1 g aluminium oxide, following partitioning of the total extract with petroleum ether (3 ml), further dilution of assay aliquots and drying. Recoveries averaged 100 and 72% for oestradiol and progesterone, respectively. Results included increases in progesterone during luteal phases or pregnancies to 7688 ng g-1 (tiger, Panthera tigris), 2594 ng g-1 (lion, P. leo), 3000 ng g-1 (cheetah, Acinonyx jubatus) and 4915 ng g-1 (caracal, Felis caracal). Faecal oestradiol peaks near oestrus included 246 ng g-1 (tiger), 175 ng g-1 (lion) 190 ng g-1 (cheetah), 23 ng g-1 (caracal) and 190 ng g-1 (domestic cat, F. catus).

Ten bulls with a scrotal circumference of less than 30 cm at the end of growth performance testing, and 10 cohorts of the same age, size and breed type with a scrotal circumference greater than 30 cm were used to evaluate if testosterone response following GnRH administration could be used to test for fertility, for semen quality, and for specific pathologic testicular parenchymal changes. Serum testosterone concentrations were determined immediately before and 2 to 3 hours following intramuscular injection of 250 ug GnRH. Bulls were examined for breeding soundness, then fertility was tested in a breeding trial; testicular histology was assessed by determining the percentage of cross-sections of seminiferous tubules with no spermatocytes. The mean (+/- SEM) post-GnRH serum testosterone concentration for all bulls was 11.71 (+/- 0.64) ng/ml. In order to examine for an association, the GnRH response was classified as above or below the mean for resultant serum testosterone concentration. The GnRH response classification was not related to the scrotal circumference, percentage of tubules devoid of spermatocytes, or percentage of progressively motile spermatozoa (P > 0.10). The percentage of morphologically normal spermatozoa was significantly higher (P < 0.05) in the bulls with a higher than mean testosterone secretion in response to GnRH injection. In the breeding trial, the percentage of heifers bred and the percentage of heifers pregnant (60 days post breeding) were not significantly different (P > 0.10) between the 2 classifications of GnRH response. The GnRH response test was related to the percentage of morphologically normal spermatozoa but did not predict fertility of yearling bulls in this study.

The intramuscular administration of adrenocorticotrophic hormone (ACTH) to calves, in either a short-acting form (cosyntrophin) or a longer-acting form (ACTHAR Gel), failed to induce any alteration in circulating fibrinogen or fibronectin values, despite marked elevations in plasma cortisol concentrations. With the longer-acting ACTH, plasma cortisol was elevated for at least 12 h following treatment and induced the expected physiological response of an elevation in blood glucose. In contrast, both forms of ACTH induced marked increases (p < 0.01) in plasma fibrinogen and fibronectin when administered to rabbits. The elevation in the circulating levels of these proteins was first observed 24 h after ACTH administration, by which time plasma corticosteroid values had returned to pre-treatment values. With both ACTH preparations the increases in the circulating levels of these proteins were sustained for at least 96 h. The results suggest that, in cattle, the well-recognized increases in plasma fibrinogen values following stress are not associated with the concomitant increase in plasma cortisol. Further, the results clearly illustrate the marked species differences in the response of acute-phase reactant proteins to elevated glucocorticoids.

Greater concentrations of androstenedione than testosterone were usually present during periods of non-musth in plasma collected weekly for various periods up to 2 years in 8 male Asian elephants (4-35 years of age). For the 6 males that exhibited musth the androstenedione/testosterone ratio shifted greatly in favour of testosterone. The severity of musth was assessed weekly using a scale of 1 to 5 for each of 8 behavioural traits including urine dribbling, temporal gland secretion and aggression. A significant correlation (P less than 0.05) was noted between plasma testosterone concentrations and the musth score value in 5 of 6 musth episodes. Brief shifts in the ratio of two androgens when testosterone predominated (n = 106) were seen during the non-musth period in 3 of the males studied continuously for 2 years. In 82% of these instances, stimuli of a sexual or aggressive nature had occurred in the preceding 48 h (chi 2, P less than 0.01). A heterologous bovine assay was used to measure LH values in plasma collected every 15 min for 12 h. Increases in testosterone concentrations followed pulsatile increases in plasma LH concentrations during 7 non-musth periods in 4 animals. Apart from pulse frequency, increases in the variables describing pulsatile LH secretion were seen in 2 strong musth and 2 mild musth episodes compared to non-musth values. A strong musth, however, was characterized by a much greater increase in pulsatile testosterone secretion than was a mild musth and which may be a function of the duration of musth.

The influence of parturition on plasma concentrations of fibrinogen, fibronectin and von Willebrand factor is compared in the canine, porcine, equine, bovine and human species. The significance of the alterations in these proteins at parturition is discussed in relation to their role in haemostasis, cellular adhesion reactions, and as acute-phase reactant proteins. The effect of hormones on the expression of these proteins in whole animal and cultured cell systems is briefly reviewed

Ovarian steroids, luteal oxytocin and uterine prostaglandin F2 alpha (PGF2 alpha) interact to regulate luteolysis in the cow. Since persistent corpora lutea are characteristic in cows with pyometra, the positive feedback relationship between oxytocin and PGF2 alpha was examined in normal animals and in cows with clinical cases of pyometra. In Experiment 1, 10 nonmilking Holstein cows were injected with oxytocin (0.33 IU/kg bodyweight, i.m.) on Days 5, 10 and 15 of the estrous cycle and the PGF2 alpha response, as reflected in plasma levels of 13,14-dihydro-15-keto-PGF2 alpha, was compared with that of 10 similarly treated Holstein cows with pyometra. Estrous cycle length and estradiol and progesterone concentrations did not appear to be affected by single injections of oxytocin on the three days in cyclic cows. Progesterone and estradiol values in cows with pyometra were similar to those of normal cows, but the peak value and the area under the curve of the pulse of PGFM resulting from oxytocin injection were significantly greater in cows with pyometra. In Experiment 2, conversely, five cyclic, lactating Holstein cows at Day 15 and seven postparturient Holstein cows with pyometra were injected with a synthetic prostaglandin (cloprostenol, 500-mu-g, i.m.) to compare oxytocin values in frequently collected blood samples. Cyclic cows treated with prostaglandin on Day 15 secreted 70.3 +/- 27.7 pg/ml/h of oxytocin, while cows with pyometra secreted 4.8 +/- 5.4 pg/ml/h (P < 0.01). The persistence of corpora lutea in cows with pyometra is not due to an insufficiency in the synthesis of uterine PGF2 alpha, but may be associated with inadequate secretion of ovarian oxytocin.