The Fusarium head blight (FHB) pathogen Fusarium graminearum produces the trichothecene mycotoxin deoxynivalenol and reduces wheat yield and grain quality. Spring wheat (Triticum aestivum) genotype CB037 was transformed with constitutive expression (CE) constructs containing sorghum (Sorghum bicolor) genes encoding monolignol biosynthetic enzymes caffeoyl coenzyme A (CoA) 3-O-methyltransferase (SbCCoAOMT), 4-coumarate-CoA ligase (Sb4CL), or coumaroyl shikimate 3-hydroxylase (SbC3 ' H) or monolignol pathway transcriptional activator SbMyb60. Spring wheats were screened for type I (resistance to initial infection, using spray inoculations) and type II (resistance to spread within the spike, using single-floret inoculations) resistances in the field (spray) and greenhouse (spray and single floret). Following field inoculations, disease index, percentage of Fusarium-damaged kernels (FDK), and deoxynivalenol measurements of CE plants were similar to or greater than those of CB037. For greenhouse inoculations, the area under the disease progress curve (AUDPC) and FDK were determined. Following screens, focus was placed on two each of SbC3 ' H and SbCCoAOMT CE lines because of trends toward a decreased AUDPC and FDK observed following single-floret inoculations. These four lines were as susceptible as CB037 following spray inoculations. However, single-floret inoculations showed that these CE lines had a significantly reduced AUDPC (P < 0.01) and FDK (P <= 0.02) compared with CB037, indicating improved type II resistance. None of these CE lines had increased acid detergent lignin compared with CB037, indicating that lignin concentration may not be a major factor in FHB resistance. The SbC3 ' H and SbCCoAOMT CE lines are valuable for investigating phenylpropanoid-based resistance to FHB.
Phytic acid (myo-inositol 1,2,3,4,5,6-hexakisphosphate) in grains and legumes reduces bioavailability and absorption of minerals in the gut via chelation of divalent minerals such as iron and zinc. In wheat (Triticum aestivum L.), a low-phytate mutant (lpa1-1), developed by ethyl methanesulfonate mutagenesis, was reported to reduce phytate in wheat grain by up to 35% and to elevate free inorganic phosphate (Pi). Little is known about the genetic architecture conditioning this high-Pi (HIP) phenotype in wheat. Inheritance of the HIP phenotype was evaluated in three segregating populations developed with the lpa1-1 derivative A02568WS-A-12-10 as a common parent. Distinct genotypic classes were not identified in these populations. To identify quantitative trait loci (QTL) and develop molecular markers for the HIP phenotype, 171 recombinant inbred lines (RILs) from a winter wheat cross 'Danby' x A02568WS-A-12-10 were phenotyped for Pi concentration and genotyped by reduced-representation sequencing. A total of 1,246 nonredundant, high-quality single-nucleotide polymorphisms (SNPs) were used to construct a linkage map spanning 3,272 cM. Two major-effect QTL were identified on chromosomes 4D and 5A, accounting for 23 and 33% of the total phenotypic variation, respectively. Multiple interval mapping (MIM) identified synergistic additive x additive epistasis between these QTL, and the full MIM model accounted for 54% of the phenotypic variation in grain Pi. Ten candidate genes were identified within or in close proximity to the genomic locations of the QTL, five of which encode proteins within the inositol phosphate metabolism pathway. Site-specific marker assays were developed for marker-assisted breeding.
Wheat streak mosaic virus (WSMV) and triticum mosaic virus (TriMV) are economically important viruses of wheat (Triticum aestivum L.), causing significant yield losses in the Great Plains region of the United States. These two viruses are transmitted by wheat curl mites, which often leads to mixed infections with synergistic interaction in grower fields that exacerbates yield losses. Development of dual-resistant wheat lines would provide effective control of these two viruses. In this study, a genetic resistance strategy employing an RNA interference (RNAi) approach was implemented by assembling a hairpin element composed of a 202-bp (404-bp in total) stem sequence of the NIb (replicase) gene from each of WSMV and TriMV in tandem and of an intron sequence in the loop. The derived RNAi element was cloned into a binary vector and was used to transform spring wheat genotype CB037. Phenotyping of T1 lineages across eight independent transgenic events for resistance revealed that i) two of the transgenic events provided resistance to WSMV and TriMV, ii) four events provided resistance to either WSMV or TriMV, and iii) no resistance was found in two other events. T2 populations derived from the two events classified as dual-resistant were subsequently monitored for stability of the resistance phenotype through the T4 generation. The resistance phenotype in these events was temperature-dependent, with a complete dual resistance at temperatures ≥25°C and an increasingly susceptible response at temperatures below 25°C. Northern blot hybridization of total RNA from transgenic wheat revealed that virus-specific small RNAs (vsRNAs) accumulated progressively with an increase in temperature, with no detectable levels of vsRNA accumulation at 20°C. Thus, the resistance phenotype of wheat harboring an RNAi element was correlated with accumulation of vsRNAs, and the generation of vsRNAs can be used as a molecular marker for the prediction of resistant phenotypes of transgenic plants at a specific temperature.
BACKGROUND Previous research has suggested that proteins and other quality parameters of wheats may have changed over a century of wheat breeding. These changes may affect protein digestibility. Therefore, in vitro protein digestibility of breads made with 21 cultivars of wheat introduced or released in the US between 1870 and 2013 were evaluated. RESULTS Protein digestibility increased with release year, but was not normally distributed; three older cultivars had significantly lower digestibility compared with the other cultivars [42.0±0.3 mol% (primary amino N/total N) versus 34.7±0.7 mol%; p<0.001]. High molecular weight (MW) protein fractions increased and low MW protein fractions decreased with release year, but these changes were not related to protein digestibility. Thus, other differences in protein composition or other flour components may contribute to diminished digestibility of the three older cultivars. CONCLUSIONS This study identified differences in protein digestibility among wheat cultivars that may have important implications in human nutrition. Further investigation is required to determine the specific characteristics that differentiate high and low digestibility wheat cultivars. This article is protected by copyright. All rights reserved.
The above-mentioned article was published in 2015 with an error in the reverse primer sequence for the PPOA2d1074 marker, which made amplification difficult. The reverse primer was missing a thymine nucleotide at the thirteenth position (GCGGTGCTTCACTTGGT).
With climate variation common in the U.S. Great Plains and particularly in Nebraska, wheat growers prefer broadly adapted cultivars. 'NE10589' (Reg. no. CV-1165, PI 675998) hard red winter wheat (Triticum aestivum L.) was developed cooperatively by the Nebraska Agricultural Experiment Station and the USDA-ARS and released in January 2015 by the developing institutions. NE10589 was released primarily as a broadly adapted semi-dwarf cultivar for its superior performance under rainfed conditions throughout Nebraska and adjacent areas of the Great Plains. Its broad adaptation ensures that it will perform well under the typical environmental fluctuations that occur in Nebraska. NE10589 was selected from the cross 'OK986977'Jagalene'//'Camelot', where the pedigree of OK98697 is 'TAM 200'/HEB313E'//'2158'. The F-2 to F-3 generations were advanced using the bulk breeding method at the Eastern Nebraska Research and Extension Center near Mead, NE, in 2006-2007. In 2007-2008, single F-3:4 head rows were grown for selection. There was no further selection within the line thereafter. The F-3:5 was evaluated as a single four-row plot at Lincoln, NE, and a single row at Mead, NE, in 2009. In 2010, it was assigned the experimental line number NE10589. NE10589 was evaluated in replicated trials thereafter. It has excellent winter survival, acceptable disease reactions to many of the common diseases in its target area, and acceptable end-use quality for bread making.
Background and objectives Little data have been reported on protein molecular weight distribution (MWD) for waxy (amylose-free) wheat (Triticum aestivum L.) despite their importance in quality. This research aimed to investigate variations of protein MWD parameters and their associations with gluten strength in winter waxy wheat. Findings Winter waxy wheat genotypes varied significantly (p < 0.001) for most protein MWD parameters including SDS-extractable polymeric protein (EPP) and unextractable polymeric protein (UPP) fractions, which are major components of gluten proteins. Allelic variation of HMW glutenin subunits had significant influence on variations of EPP and UPP in waxy genotypes. Negative correlations for EPP and positive correlations for UPP were observed with mixograph peak time and gluten index for wheat genotypes. In particular, highly significant (p < 0.001) correlations were observed between mixograph peak time and the proportion of EPP in total protein for waxy genotypes. Conclusions Winter waxy wheat genotypes showed significant variations for EPP and UPP parameters, which were associated with variation of gluten strength. Significance and novelty: This research identified variation of protein MWD parameters and their associations with gluten strength. The information should be valuable in segregating winter waxy wheat genotypes that have favorable gluten quality as well as unique waxy starch.
Fusarium head blight (FHB) is among the most common fungal diseases affecting wheat, resulting in decreased yield, low-density kernels, and production of the mycotoxin deoxynivalenol, a compound toxic to humans and livestock. Human visual analysis of representative wheat samples has been the traditional method for FHB assessment in both official inspection and plant breeding operations. While not requiring specialized equipment, visual analysis is dependent on a trained and consistent workforce, such that in the absence of these aspects, biases may arise among inspectors and evaluation dates. This research was intended to avoid such pitfalls by using longer wavelength radiation than the visible using hyperspectral imaging (HSI) on individual kernels. Linear discriminant analysis models to differentiate between sound and scab-damaged kernels were developed based on mean of reflectance values of the interior pixels of each kernel at four wavelengths (1100, 1197, 1308, and 1394 nm). Other input variables were examined, including kernel morphological properties and histogram features from the pixel responses of selected wavelengths of each kernel. The results indicate the strong potential of HSI in estimating fusarium damage. However, improvement in aligning this procedure to visual analysis is hampered by the inherent level of subjectivity in visual analysis.
‘LCS Compass’ (Reg. No. CV‐1149, PI 675458), a hard red winter (HRW) wheat ( Triticum aestivum L.), was developed and tested as VA10HRW‐13 and co‐released by the Virginia Agricultural Experiment Station and Limagrain Cereal Seeds, LLC, in 2015. LCS Compass was derived from the cross ‘Vision 20’ /‘Stanof’ using a modified bulk breeding method. LCS Compass is a widely adapted, high‐yielding, awned, semidwarf ( Rht1 ) HRW wheat with early to medium maturity and resistance or moderate resistance to diseases prevalent in the mid‐Atlantic and Great Plains regions of the United States. In the 2013 Uniform Bread Wheat Trial conducted over 18 locations in eastern states, LCS Compass produced an average grain yield of 4609 kg ha −1 that was similar to ‘Vision 30’ (4697 kg ha −1 ). In the northern Great Plains, the average grain yield of LCS Compass (4015 kg ha −1 ) over 44 locations in 2013 was similar to ‘Jerry’ (4013 kg ha −1 ). In the South Dakota crop zone 3 variety test, LCS Compass had a 3‐yr (2015–2017) yield average of 5575 kg ha −1 and was one of highest‐yielding cultivars among the 19 cultivars tested over the 3‐yr period. LCS Compass has good end‐use quality in both the eastern and Great Plains regions of the United States.
‘Matterhorn’ (Reg. No. CV‐1151, PI 687896) hard white winter waxy wheat (Triticum aestivum L.) was developed cooperatively by the USDA‐ARS and the Nebraska Agricultural Experiment Station and released in 2018. Matterhorn, a sibling of the hard red waxy cultivar Mattern, has white grain color and waxy (amylose‐free) endosperm starch. It was released primarily for its unique end‐use quality attributes and for grain yield competitiveness with currently grown Nebraska‐adapted cultivars. The waxy starch is conditioned by the presence of three naturally occurring mutations that eliminate production of the enzyme granule‐bound starch synthase. Granule‐bound starch synthase synthesizes amylose in typical wheats and other cereal crops. Matterhorn (tested as NX04Y2107W) was selected from the heterogeneous red/white‐seeded experimental line NX04Y2107 derived from the cross NW98S061/99Y1442.
Progress in plant breeding programs is the result of creating and selecting new lines with novel allele combinations that perform better than their parents. This year‐on‐year improvement is known as genetic gain and is a function of genetic diversity, selection accuracy, selection intensity, and selection cycle time. To estimate the gain in wheat (Triticum aestivum L.) breeding in the US Central Plains, lines that were submitted to the collaborative Southern Regional Performance Nursery (SRPN) between 1992 and 2014 were grown in a common nursery for 3 yr at two locations in a single‐replicate augmented block design. Moderate to high broad‐sense heritability was observed for plant height (H2 = 0.88), heading date (H2 = 0.79), and grain yield (H2 = 0.41). From the common grow‐out, genetic gain for yield over the time period was estimated at 1.1% yr−1, whereas individual breeding program genetic gain varied between 0.3 and 1.9% yr−1. Increases in Kansas state on‐farm yields during the same period showed a nonsignificant trend of 0.13% yr−1 with large year‐to‐year variation. These results suggest that although progress is being made in US Central Plains breeding programs, a yield gap remains that could be attributable to genetic progress not being realized in on‐farm production.
Rye (Secale cereale L.) chromosome arm 1RS has been used world-wide by wheat (Triticum aestivum L.) breeding programs as a source of pest- and pathogen-resistance genes, and to improve grain yield and stress tolerance. The most common vehicles used to access 1RS are various 1AL.1RS and 1BL.1RS wheat-rye chromosomal translocations. Over the past 25 years, advanced North American wheat breeding lines were evaluated, first by assay of secalin storage proteins, and later by use of DNA marker TSM0120, for the presence of these two translocations. Both methods provide accurate and efficient means of identifying and differentiating 1BL.1RS and 1A.1RS. Both 1Al.1RS and 1BL.1RS wheats were found in all tested years. 1AL.1RS lines were more common in southern Great Plains breeding programs. 1AL.1RS lines were released as cultivars at a frequency identical to that of wild-type breeding lines. In contrast, 1BL.1RS breeding lines were developed by breeding programs throughout the Great Plains, but fewer were released as cultivars. Both 1RS translocation types persist in Great Plains breeding programs. The lower rate of release of 1BL.1RS cultivars no doubt is a consequence of the more drastic effects on breadmaking quality relative to those observed with 1AL.1RS.
‘TAM 204’ (Reg. no. CV‐1155, PI 686859), a hard red winter wheat (Triticum aestivum L.) cultivar with the experimental designation of TX06V7266, was developed and released by Texas A&M AgriLife Research in 2014. TAM 204 is an F4–derived line from the cross ‘TAM 112’/TX01M5009 made at Vernon, TX, in 2001. TAM 204 is an apically awnletted, medium‐maturing, semidwarf wheat with red glumes. It was released primarily as a grain, dual‐purpose, and graze‐out wheat with high grain and forage yield potential. It has good acid soil tolerance, is resistant to Soil‐borne wheat mosaic virus, Wheat streak mosaic virus and its vector wheat curl mite, greenbug, stem rust and stripe rust, and it is moderately susceptible to leaf rust. TAM 204 has a high level of resistance to Hessian fly biotypes GP and vH9 and a moderate level of resistance to biotype vH13. Compared with ‘TAM 111’, which is currently the most widely grown cultivars in Texas, TAM 204 has significantly higher grain yield in the Texas High Plains under both irrigated and dryland environments. Compared with ‘TAM 401’ and ‘Weathermaster 135’, which are currently the two most popular apically awnletted cultivars in Texas, TAM 204 has similar forage yield but higher grain yield across a wide range of environments. TAM 204 could be a better option for wheat growers and cattle owners under grain‐only, dual‐purpose (grazing‐plus‐grain), and graze‐out production system in the southern US Great Plains.
The development of inexpensive, whole-genome profiling enables a transition to allele-based breeding using genomic prediction models. These models consider alleles shared between lines to predict phenotypes and select new lines based on estimated breeding values. This approach can leverage highly unbalanced datasets that are common to breeding programs. The Southern Regional Performance Nursery (SRPN) is a public nursery established by the USDA-ARS in 1931 to characterize performance and quality of near-release wheat ( L.) varieties from breeding programs in the US Central Plains. New entries are submitted annually and can be re-entered only once. The trial is grown at >30 locations each year and lines are evaluated for grain yield, disease resistance, and agronomic traits. Overall genetic gain is measured across years by including common check cultivars for comparison. We have generated whole-genome profiles via genotyping-by-sequencing (GBS) for 939 SPRN entries dating back to 1992 to explore the potential use of the nursery as a genomic selection (GS) training population (TP). The GS prediction models across years (average = 0.33) outperformed year-to-year phenotypic correlation for yield ( = 0.27) for a majority of the years evaluated, suggesting that genomic selection has the potential to outperform low heritability selection on yield in these highly variable environments. We also examined the predictability of programs using both program-specific and whole-set TPs. Generally, the predictability of a program was similar with both approaches. These results suggest that wheat breeding programs can collaboratively leverage the immense datasets that are generated from regional testing networks.
Hexaploid waxy wheat (Triticum aestivum L.) has null mutations in Wx genes and grain lacking amylose with increased digestibility and usability for specialty foods. The waxy cultivar Mattern is susceptible to Fusarium head blight (FHB) caused by Fusarium graminearum species complex, which produces the mycotoxin deoxynivalenol (DON). In experiment 1, conducted during low natural FHB, grain from waxy breeding lines, Mattern, and wild-type breeding lines and cultivars were assessed for Fusarium infection and DON concentration. Nine Fusarium species and species complexes were detected from internally infected (disinfested) grain; F. graminearum infections were not different between waxy and wild-type. Surface- and internally infected grain (nondisinfested) had greater numbers of Fusarium isolates across waxy versus wild-type, but F. graminearum-like infections were similar; however, DON levels were higher in waxy. In experiment 2, conducted during a timely epidemic, disease severity, Fusarium-damaged kernels (FDK), and DON were assessed for waxy breeding lines, Mattern, and wild-type cultivars. Disease severity and FDK were not significantly different from wild-type, but DON was higher in waxy than wild-type lines. Across both experiments, waxy breeding lines, Plant Introductions 677876 and 677877, responded similarly to FHB as moderately resistant wild-type cultivar Overland, showing promise for breeding advanced waxy cultivars with reduced FHB susceptibility.
Reduced or low phytic acid (LPA) mutants of wheat (Triticum aestivum L.) increase the bioavailability and, subsequently, the gut absorption of minerals in monogastric animals, including humans. The USDA–ARS in cooperation with the University of Nebraska developed and released eight low phytate (LPA) winter wheat lines (N16MD9012 [Reg. No. GP‐1024, PI 682715], N16MD9140 [Reg. No. GP‐1025, PI 682717], N16MD9275 [Reg. No., GP‐1026, PI 682718], N16MD9268 [Reg. No. GP‐1027, PI 682719], N16MD9046 [Reg. No. GP‐1028, PI 682720], N16MD9204 [Reg. No. GP‐1029, PI 682722], N16MD9074 [Reg. No. GP‐1030, PI 682723], and N16MD9153 [Reg. No. GP‐1031, PI 682724]) adapted to the Great Plains of North America. The highest‐yielding LPA line was not significantly different in grain yield from the adapted controls ‘Anton’ and ‘Intrada’, was significantly higher in grain yield than ‘Big Sky’ and ‘Siouxland’, but was significantly lower than the two highest‐yielding controls, ‘Freeman’ and ‘Ruth’. Overall, there were no significant differences in grain yield, grain volume weight, and grain protein concentration (GPC) between the LPA lines and the mean values of the adapted controls. On average, the LPA lines had 18% more zinc than the adapted controls. Six of the eight LPA lines contain the Lr37/Sr38/Yr17 genes combination, which provides resistance for leaf, stem, and yellow/stripe rust. Grain yield data obtained in diverse environments in Nebraska indicated no grain yield reduction associated with the low phytate trait.
Recombinant inbred lines (RILs) of winter wheat (Triticum aestivum L.) were used to determine whether the combination of low grain phytate (LPA) conditioned by lpa1‐1 and Gpc‐B1 (where GPC stands for grain protein content) alleles would simultaneously increase beneficial mineral concentrations and grain protein without pleiotropic effects on grain yield. Four different genotypes (LPA‐GPC, LPA‐wild type [WT], WT‐GPC, or WT‐WT) were used as treatments in field experiments in Nebraska. Genotypic effects on senescence, grain yield, grain volume weight, grain protein, Fe, Zn, and other mineral grain concentrations were determined. Low grain phytate alone and in combination with GPC increased dialyzed Zn, Ca, and Mn. Gpc‐B1 had a slight effect on grain protein concentration in the tested genetic backgrounds and environments. The combination of LPA and GPC did not lower grain yield, grain protein, or total grain Fe and Zn concentrations. However, the LPA‐GPC combination significantly reduced grain volume weight. The LPA allele alone reduced grain protein concentration. Introgression of lpa1‐1 alleles into adapted Great Plains winter wheat materials can improve dialyzed Zn, Ca, and Mn concentrations without reducing grain yield and, coupled with introgression of Gpc‐B1, provide more nutritious wheat kernels.
Recent development of hard winter waxy (amylose-free) wheat adapted to the North American climate has prompted the quest to find a rapid method that will determine mixture levels of conventional wheat in lots of identity preserved waxy wheat. Previous work documented the use of conventional near infrared (NIR) reflectance spectroscopy to determine the mixture level of conventional wheat in waxy wheat, with an examined range, through binary sample mixture preparation, of 0–100% (weight conventional / weight total). The current study examines the ability of NIR hyperspectral imaging of intact kernels to determine mixture levels. Twenty-nine mixtures (0, 1, 2, 3, 4, 5, 10, 15, …, 95, 96, 97, 98, 99, 100%) were formed from known genotypes of waxy and conventional wheat. Two-class partial least squares discriminant analysis (PLSDA) and statistical pattern recognition classifier models were developed for identifying each kernel in the images as conventional or waxy. Along with these approaches, conventional PLS1 regression modelling was performed on means of kernel spectra within each mixture test sample. Results indicated close agreement between all three approaches, with standard errors of prediction for the better preprocess transformations (PLSDA models) or better classifiers (pattern recognition models) of approximately 9 percentage units. Although such error rates were slightly greater than ones previously published using non-imaging NIR analysis of bulk whole kernel wheat and wheat meal, the HSI technique offers an advantage of its potential use in sorting operations.
Background and objectivesFlour and starch from waxy wheat (Triticum aestivum L) have unique properties such as high water-holding capacity, low pasting temperature, low syneresis, and high resistance to retrogradation. However, the milling performance of waxy wheat was not well documented. In this study, the milling quality of a waxy wheat (Mattern) was evaluated by comparing its flour yield, chemical composition, flow properties, and particle size distribution with that of two wild-type hard red winter (HRW) wheats (Wesley and Everest) using two laboratory milling methods. The effects of moisture level and tempering time on milling behavior for the waxy wheat (Mattern) were examined and compared with that of wild-type wheat. FindingsWaxy wheat (Mattern) seemed to require more moisture during tempering than wild-type wheats to achieve a similar drop in SKCS hardness. For Mattern, the overall grain hardness drop was minimal when tempering to moisture contents below 17.5%. When the tempering moisture content increased from 13% to 17.5%, the flour yield increased, but decreased after 17.5% moisture content. Tempering Mattern to 16% and higher resulted in a higher bran yield compared with shorts yield, although the yield of the shorts from Mattern was significantly greater than that of the shorts from both wild-type wheats. The flour yield of Mattern was 65.1%, which was significantly lower than that of both wild-type wheats (73.1% for Wesley and 72.2% for Everest). ConclusionsAt the same moisture content (16%), the hardness of the waxy wheat (Mattern) kernel decreased at a slower rate than the wild-type wheat during tempering for 48hr. The flour yield of the waxy wheat (Mattern) was significantly lower than that of wild-type wheat. Mattern flour was less free-flowing and more adhesive than the wild-type HRW flour, mainly due to its smaller particle size and higher fat content. Significance and noveltyHigher optimal tempering moisture was needed to mill the waxy wheat (Mattern), and the yield of waxy wheat flour was maximized when the kernels were tempered at 17.5% moisture for 24hr.
‘TAM 114’ (Reg. No. CV‐1146, PI 686860), a hard red winter wheat (Triticum aestivum L.) cultivar with the experimental designation of TX07A001505, was developed and released by Texas A&M AgriLife Research in 2014. TAM 114 is an F6–derived line from the cross ‘TAM 111’/TX98A0050 made at Bushland, TX, in 1999. TAM 114 is an awned, medium‐maturing, semidwarf wheat with red glumes. It was released primarily for its extra‐strong baking properties as indicated by longer bake mix time, excellent mixing tolerance, and good loaf volume. TAM 114 has significantly higher grain yield than that of TAM 111 and ‘TAM 112’ in the Texas High Plains under both irrigated and dryland environments. It is high in grain volume weight, is resistant to leaf, stripe, and stem rusts, has a moderate level of resistance to Hessian fly biotypes GP and vH9, and has good acid soil tolerance. Compared with TAM 111 and TAM 112, which are currently the two most widely grown cultivars in Texas, TAM 114 has higher grain yield, higher grain volume weight, better leaf and stripe rust resistance, and better bread baking properties. It will provide a good complement to other hard red winter wheat cultivars for wheat producers, millers, and bakers and ultimately for consumers of wheat products in the southern US Great Plains.