Canine parvovirus (CPV) remains one of the most significant viral pathogens in veterinary medicine due to its high transmissibility and continuous genetic evolution. Despite more than four decades of intensive research, a data-driven assessment of how global research priorities align with the virus's evolving epidemiological and evolutionary dynamics is still lacking. This study presents a comprehensive scientometric analysis of global CPV research published between 1980 and 2024, aiming to identify dominant research themes, collaboration structures, and critical knowledge gaps. A total of 818 publications indexed in the Web of Science Core Collection were analysed using the Bibliometrix R package. The findings reveal a highly centralised but stable research ecosystem, predominantly led by the United States and Italy, with a strong emphasis on molecular characterisations, VP2 gene variants, and vaccine-related studies. Thematic evolution analysis demonstrates a clear transition from early clinicopathological descriptions toward molecular epidemiology and evolutionary surveillance. However, a key imbalance emerges: although CPV research increasingly adopts a One Health perspective, scientific output remains disproportionately concentrated in a limited number of high-income regions, systematically under-representing areas with potentially high disease burden and surveillance needs. In conclusion, this study maps the historical and conceptual evolution of CPV research while explicitly identifying neglected domains in global surveillance and geographical coverage. These findings provide a strategic evidence base for researchers, funding agencies, and policy stakeholders to recalibrate research priorities toward emerging variants, under-investigated regions, and integrated One Health-oriented surveillance frameworks.

Among dogs with hyperadrenocorticism (HAC), thrombocytosis is frequently reported and is considered to be associated with a hypercoagulable state. Substantial and easily accessible haematological evidence such as platelet count (PLT), plateletcrit (PCT), mean platelet volume (MPV) and platelet distribution width (PDW) indicating hypercoagulability may serve as a basis for considering therapeutic management. This study was performed on 22 dogs diagnosed with uncomplicated pituitary-dependent hyperadrenocorticism (PDH). Twelve dogs were assigned to the control group by computer-based randomisations. A series of standard clinical procedures in all dogs, including clinical examination, blood analyses, urinalysis and abdominal ultrasonography were performed. Platelet-related indices such as PLT, PCT, MPV and PDW were also evaluated at the time of admission in all dogs. Dogs were excluded if they had a secondary disease or hypertension (≥160 mmHg). Although no statistical significance was present, PLT, PCT and PDW were higher in PDH dogs compared to healthy controls. In addition, MPV was lower in PDH dogs (P < 0.05). In conclusion, exhausted bone marrow activity associated with the chronicity of HAC may demonstrate lower MPV values in dogs with PDH. Further studies evaluating the relationship between platelet parameters obtained by whole blood samples and PDH dogs are needed.

Feline chronic kidney disease (CKD) is a common condition in cats. International guideline adherence may vary among veterinary practitioners. The objective of the study is to evaluate the diagnostic, therapeutic and monitoring practices of primary care veterinarians in Turkey regarding feline CKD and to compare these practices with guidelines. A cross-sectional online questionnaire consisting of 32 items was distributed to veterinarians. The survey addressed demographics, diagnostic approaches, treatment modalities and monitoring strategies for feline CKD cases. Descriptive statistics were calculated to analyse responses. A total of 281 veterinarians participated. While 93.6% were aware of IRIS guidelines, only 55.2% reported performing all recommended diagnostic procedures. SDMA was used alongside creatinine by 73.6% of respondents. However, only 30.5% routinely measured blood pressure. Telmisartan and ACE inhibitors were commonly used, but amlodipine was prescribed by only 8.9%. Most veterinarians recommended renal diets irrespective of CKD stage and typically suggested a short 1–2 week transition period. Follow-up intervals varied, with 40.9% recommending re-evaluations every 2–3 months. Although awareness of international guidelines is high, discrepancies remain in disease substaging, blood pressure monitoring and antihypertensive drug selection. Greater emphasis on blood pressure measurement and improved access to feline-licensed drugs may enhance CKD management in cats.

BACKGROUND:The invasive nature of liver biopsy and the risk of complications make patients reluctant to undergo the procedure. This situation has necessitated the search for easily accessible haematological indexes such as neutrophil-to-lymphocyte ratio (NLR) and red cell distribution width to lymphocyte ratio (RDW/LYM) to contribute to the clinical diagnosis and prognosis evaluation processes in dogs with cholangitis-cholangiohepatitis. OBJECTIVES:The aim of the study is to evaluate some predictive markers to contribute the clinical diagnosis in dogs with cholangitis-cholangiohepatitis. MEDHODS:Seventeen client-owned dogs with cholangitis-cholangiohepatitis were randomly allocated. Nine clinically healthy dogs (control group) confirmed with clinical examination, complete blood count (CBC) and serum profiles were used. Cholangitis-cholangiohepatitis was diagnosed in dogs with dilated bile ducts, thickened gallbladder wall up to 4 mm and increased parenchymal echogenicity. Clinical procedures including clinical examination, blood analyses and diagnostic abdominal ultrasonography were performed in all dogs. CBC indices such as NLR, mean platelet volume to platelet ratio (MPV/PLT), red cell distribution width to platelet ratio (RDW/PLT), monocyte-to-lymphocyte ratio (MLR), platelet-to-lymphocyte ratio (PLR) and RDW/LYM were calculated using absolute CBC values. RESULTS:A final total of 10 dogs were enrolled in the study. NLR and RDW/LYM ratios were significantly higher (p < 00.5) in dogs with cholangitis-cholangiohepatitis compared to healthy ones. CONCLUSION:The results of this study suggest that the use of NLR and RDW/LYM ratio in dogs with cholangitis-cholangiohepatitis complex appears potentially promising. Larger prospective, case-control studies are needed to further evaluate these findings. Veterinary practitioners can obtain valuable diagnostic data before possible invasive liver procedures by evaluating NLR and RDW/LYM ratios, which are easily obtained from CBC parameters.

Bu çalışmada, parvoviral enteritisli köpeklerde sepsis ve SİYS'e bağlı miyokardiyal fonksiyon değişiklikleri ile standart ekokardiyografi ve iki boyutlu benek takibi ekokardiyografi (2DBTE) yöntemlerinin etkinliği karşılaştırılmıştır. Bulguların, kardiyak komplikasyonların erken teşhisine katkı sağlaması amaçlanmıştır. Çalışmaya, CPV enfeksiyonu teşhisi konulan ve SİYS kriterlerinden en az ikisini gösteren 16 yavru köpek (Grup A) ile sağlıklı 16 yavru köpek (Grup B) dâhil edildi. Grup A ve Grup B’de bulunan köpeklerden bir kez kan alınarak hematolojik muayene gerçekleştirildi. Her iki gruptaki yavru köpeklere standart ekokardiyografi ve 2DBTE uygulandı. 2DBTE ile sol ventrikül radyal gerilimi ve gerilim hızını belirlemek için görüntüler kaydedildi ve çevrimdışı analiz edildi. Grup A ve Grup B’nin radyal gerilim ve gerilim hızları kıyaslandığında istatistiksel olarak anlamlı bir farka rastlanmadı. Ancak Grup A’da ölen hayvanların sağlıklı hayvanlara kıyasla daha düşük radyal gerilim değerlerine sahip olduğu belirlendi. Standart ekokardiyografi ile belirlenen ejeksiyon fraksiyonunun (EF) ve fraksiyonel kısalmanın (FS) gruplar arasında fark oluşturmadığı görüldü. Sepsis ve SİYS görülen parvoviral enteritisli yavru köpeklerde sol ventrikül fonksiyonlarının etkilendiği ve bu fonksiyonların değerlendirilmesinde standart ekokardiyografi ile 2DBTE metodunun da kullanılması gerektiği kanısına varıldı.

The aim of this study was to evaluate the association between spontaneous echocardiographic contrast (SEC) and left atrial (LA) parameters such as size, volume, and function in cats with hypertrophic cardiomyopathy (HCM). Cats were assigned into following groups: clinically healthy cats (n=8), HCM without SEC (n=12), and HCM with SEC (n=8). Left atrial shortening fraction (LAFS%) and left atrial fractional area change (LAFAC) had statistical significance between groups. In conclusion, compared to the healthy individuals, the presence of a decreased trend in levels of LAFS% and LAFAC in HCMSEC cats could be a predictive marker for the thromboembolic risk assessment. Further studies enrolling the asymptomatic HCM cats need to be conducted to define a cut-off value of LAFS% and LAFAC before the SEC formation.

In this study, pre-chemotherapy hematological values of 14 dogs diagnosed with diffuse B-cell small lymphocytic lymphoma were compared with the hematological data of 26 healthy dogs. Neutrophil/lymphocyte ratio (NLR), lymphocyte/monocyte ratio (LMR), platelet/lymphocyte ratio (PLR), and platelet/neutrophil ratio (PNR) were evaluated between two groups. Anemia and an increased total leukocyte count were observed in dogs with lymphoma compared to healthy ones. The PNR value was found to be significantly lower in dogs with lymphoma. It was concluded that more comprehensive studies are needed to clearly understand the diagnostic and prognostic importance of hematological parameters in B-cell small lymphocytic lymphoma of dogs.

Cardiovascular complications are the major cause of diabetes mellitus-related morbidity and mortality. Increased renin–angiotensin–aldosterone system activity and decreased β-adrenergic receptor (β-AR) responsiveness contribute to diabetic cardiac dysfunction. We evaluated the effect of sacubitril/valsartan (neprilysin inhibitor plus angiotensin receptor antagonist combination) and valsartan treatments on the diabetic cardiac function through β-AR responsiveness and on protein expression of diastolic components. Six-week-old male Sprague Dawley rats were divided into control, diabetic, sacubitril/valsartan (68 mg/kg)-, and valsartan-treated (31 mg/kg) diabetic groups. Diabetes was induced by a high-fat diet plus low-dose streptozotocin (30 mg/kg, intraperitoneal). After 10 weeks of diabetes, rats were treated for 4 weeks. Systolic/diastolic function was assessed by in vivo echocardiography and pressure–volume loop analysis. β-AR-mediated responsiveness was assessed by in vitro papillary muscle and Langendorff heart experiments. Protein expression of sarcoplasmic reticulum calcium ATPase2a, phospholamban, and phosphorylated phospholamban was determined by Western blot. Sacubitril/valsartan improved ejection fraction and fractional shortening to a similar extent as valsartan alone. None of the treatments affected in vivo diastolic parameters or the expression of related proteins. β1-/β2-AR-mediated responsiveness was partially restored in treated animals. β3-AR-mediated cardiac relaxation (an indicator of diastolic function) responses were comparable among groups. The beneficial effect of sacubitril/valsartan on systolic function may be attributed to improved β1-/β2-AR responsiveness.

Cardiovascular complications are the major cause of diabetes-related morbidity and mortality. Increased renin-angiotensin-aldosterone system activity and decreased β-adrenergic receptor (β-AR) responsiveness contribute to diabetic cardiac dysfunction. We evaluated the effect of sacubitril/valsartan (neprilysin inhibitor plus angiotensin receptor antagonist combination), and valsartan treatments on the diabetic cardiac function through β-AR responsiveness, and on protein expression of diastolic components. 6-week-old male Sprague-Dawley rats were divided into control, diabetic, sacubitril/valsartan (68 mg/kg), and valsartan treated (31 mg/kg) diabetic groups. Diabetes was induced by high fat diet plus low dose streptozotocin (30 mg/kg, intraperitoneal). After 10 weeks of diabetes, rats were treated for 4 weeks. Systolic/diastolic function was assessed by in vivo echocardiography and pressure-volume loop analysis. β-AR mediated responsiveness was assessed by in vitro papillary muscle and Langendorff heart experiments. Protein expression of sarcoplasmic reticulum calcium ATPase2a, phospholamban, and phosphorylated phospholamban was determined by Western blot. Sacubitril/valsartan improved ejection fraction and fractional shortening to a similar extent as valsartan alone. None of the treatments affected in vivo diastolic parameters or expression of related proteins. β1-/β2-AR mediated responsiveness was partially restored in treated animals. β3-AR mediated relaxation responses were comparable among groups. The beneficial effect of sacubitril/valsartan on systolic function may be attributed to improved β1-/β2-AR responsiveness.

A 10-year-old male Cocker Spaniel was presented for evaluation of right forelimb lameness and seizure-like episodes. Panting with increased respiratory rate and opisthotonus were evident during the physical examination. Cardiac auscultation revealed a left basilar, grade III/VI systolic murmur. The dog was stabilized with diazepam, fluid therapy, and oxygen. Indirect arterial blood pressure measured in the left forelimb by Doppler technique revealed no abnormalities. Thoracic radiography indicated a noticeable bulge in the area of the ascending aortic arch. Transthoracic echocardiography revealed marked aortic dilatation with a mobile, floating tissue flap dividing the aorta into two lumens. Additional diagnostic studies (computerized tomography, cardiac catheterization, and angiography) were offered but not obtained. Medical management included therapy with enalapril and clopidogrel. Clinical signs, including the right forelimb lameness and seizures, resolved within 24 h. This report represents an unusual case of aortic dissection in a dog associated with neurological signs.

Interest in green iguanas (Iguana iguana) as pet animals is increasing day by day. Compared to other pet animals, the diagnosis and treatment methods of reptilian diseases are limited. Considering that these diseases will also concern public health, new research is required. Hirstiellosis is a zoonotic ectoparasitic disease seen in green iguanas (Iguana iguana). Recommended therapies are mostly empirical, some of which can be low-impact or toxic. The objective of this study is to determine the effectiveness of ivermectin solution (5 mg/l) in the treatment of hirstiellosis and to provide preliminary data for further studies. Ivermectin solution was found to be effective in the treatment of three iguanas with the aforementioned disease, and no clinical side effects were found in the control examinations made at regular intervals.

Interest in green iguanas (Iguana iguana) as pet animals is increasing day by day. Compared to other pet animals, the diagnosis and treatment methods of reptilian diseases are limited. Considering that these diseases will also concern public health, new research is required. Hirstiellosis is a zoonotic ectoparasitic disease seen in green iguanas (Iguana iguana). Recommended therapies are mostly empirical, some of which can be low-impact or toxic. The objective of this study is to determine the effectiveness of ivermectin solution (5 mg/l) in the treatment of hirstiellosis and to provide preliminary data for further studies. Ivermectin solution was found to be effective in the treatment of three iguanas with the aforementioned disease, and no clinical side effects were found in the control examinations made at regular intervals.

Retrospective evaluation of the cardiopulmonary data is the main point in prevention and treatment of the diseases.In this study, it was aimed to retrospectively evaluate the data of 570 client-owned patients (321 dogs and 249 cats) referred to cardiology unit.The clinical findings, electrocardiographic data, echocardiography and blood analyses were evaluated.Acquired heart disease in 214 patients (37.54%), congenital heart disease in 38 patients (6.6%), and diseases affecting the lower and/or upper respiratory tract in 98 patients (17.19%) were found.Dilated cardiomyopathy was the most common acquired heart disease, and patent ductus arteriosus was the most common congenital heart disease in dogs.In cats, hypertrophic cardiomyopathy was the most common acquired heart disease, while tricuspid valve dysplasia was the most common congenital heart disease.It was concluded that clinical findings, physical examination and diagnostic applications should be evaluated together in the diagnosis of cardiopulmonary diseases.

Feline dilated cardiomyopathy (DCM) is a rare disease characterized by myocardial failure and systolic dysfunction. Taurine deficiency is the most important cause of DCM phenotype in cats. A 2 year-old Scottish fold and a 8 month-old domestic shorthair cat were referred to hospital with anorexia, weakness and increased respiratory effort. Based on electrocardiographic, radiographic and echocardiographic findings, the both cases were diagnosed with DCM. The cases were managed with pimobendan, furosemide, acetylsalicylic acid and taurine. The cats were more active and clinically healthy during 15 days follow-up. No remarkable changes were observed in follow up echocardiographies. The cases presented here reflect the clinical signs, cardiological examination findings, diagnosis and management of idiopathic DCMin 2 cats.

Squamous cell carcinomas are malign tumors composed of squamous epithelium of skin and mucous membranes and showing squamous differentiation. These tumors constitute 60-70% of malignant tumors in the cats. Environmental factors that predispose to the development of oral squamous cell carcinoma in cats are defined as exposure to cigarette smoke, the use of flea collar, and nutrition with tuna fish-containing ingredients. A male, 11-year-old Chinchilla race cat was brought to Ankara University, Veterinary Faculty, Veterinary Hospital, Department of Internal Diseases with complaints of anorexia, weakness, dysphagia, fatigue and difficulty in swallowing solid food. At the physical examination, it was determined that the tongue was deviated to the left side and kept in the same position continuously, and the right side of the tongue was thickened. At general anesthesia, a biopsy was taken from the bulk at the root of the tongue with fine needle biopsy. The diagnosis of squamous cell carcinoma was confirmed.