An innermetalic chelated azo-dye complex of second order having the stoichiometry (Cu (L1)2, 2H2O) with ligand L1,(E)-4-(2-(4-methoxyphenyl) diazenyl) benzene 1, 3 diol, has been sythesised at pH =7.48 and studied in both solid state and aqueous phase using different spectroscopic techniques.. The structure of the complex has been carried out from elemental analysis, UV-Vis, IR, and mass spectrophotometry. Thermal decomposition has been studied from TGA-DSC analysis. Cyclic voltammetry of the complex has been carried out to ensure the presence of Cu (II) in the complex (Cu(L1)2,2H2O). Magnetic susceptibility determination of (Cu (L1)2, 2H2O) complex has been taken at a constant magnetic field of 5 KG with powder sample in the temperature range 20-35K. In addition special attention has been given to the studies of ct-DNA binding to the complex (Cu(L1)2, 2H2O) by absorption spectroscopy and cyclic voltammetry measurements under physiological condition (pH=7.4, 25°C) with intrinsic binding constant in the order of 10 4 M -1 .The complex also shows higher antibacterial activity than L1.

Co(III) obtained by persulphate oxidation of Co(II) forms a chelated complex with L1, a sythesised azo-dye. (already reported in J. Indian Chem Soc. Vol. 92 January 2015 pp. 43-50). Formation of the complex has been studied in both solid state and aqueous phase using different spectroscopic techniques. Co(III) form 1:3 metal ligand complex with L1 at pH = 7.528. Thermal decomposition has been predicted from TGA-DSC analysis. Cyclic Voltametry of this complex has been studied to ensure the presence of Co (III) in complex (Co(L1)3). The complex might be an innermetalic complex of 2 nd order. Magnetic susceptibility of the complex Co(L1)3 has been taken at a constant magnetic field of 5 KG with powder sample in the temperature range 20- 35K.The complex also shows higher antibacterial activity 0than L1.The structure of the complex has been predicted from elemental analysis, UV-Vis, IR, Mass spectrophotometry.

Two novel azo-dyes namely (E)-4-(2-(4-methoxyphenyl)diazenyl)benzene 1,3-diol (L-1) and (E)-1-(2-(4-hydroxyphenyl)diazenyl)-2-naphthol (L-2) have been synthesized by the usual method of diazotisation followed by coupling reaction it pH. 7.5. The synthesized dyes are characterized by elemental analysis and UV-Vis, IR, H-1 NMR, mass spectrophotometry. These synthesised compounds are found to exhibit significant dyeing property on bleached jute substrate under neutral and particularly basic pH condition with good colour yield And wash fastness. Moreover the acid base indicator property of L-2 being more prominent than that Of L-1, the parameters measuring the performance of the former as an indicator have also been evaluated spectrophotometrically and condutometrically in this paper. The study shows better performance of L-2 than methyl orange, particularly for titration of dilute solutions.The antibacterial activity of both L-1 and L-2 has also been studied, where L-2 showed more significant effect than L-1.