Irrigation practices and groundwater levels are critical factors contributing to soil salinization in arid and semi-arid regions. However, the impact of soil salinization resulting from Yellow River water irrigation and recharge on microbial communities and their functions in the Huinong District has not been thoroughly documented. In this study, high-throughput sequencing technology was employed to analyze the diversity, composition, and structure of bacterial and fungal communities across a gradient of salinized soils. The results indicated that the alpha diversity of bacterial communities was significantly higher in slightly saline soils compared to highly saline soils. Soil salinization notably influenced the composition of both bacterial and fungal communities. Highly salinized soils were enriched with bacterial taxa such as Halomonas, Salinimicrobium, Pseudomonas, Solibacillus, and Kocuria, as well as fungal taxa including Emericellopsis, Alternaria, and Podospora. In these highly saline soils, bacterial taxa associated with iron respiration, sulfur respiration, and hydrocarbon degradation were more prevalent, whereas fungal taxa linked to functions such as soil animal pathogens, arbuscular mycorrhizal symbiosis, endophytes, dung saprotrophy, leaf saprotrophy, soil saprotrophy, fungal parasitism, and plant pathogenicity were less abundant. Random forest analysis identified nine bacterial and eighteen fungal taxa as potential biomarkers for salinity discrimination in saline soils. Symbiotic network analysis further revealed that soil salinization pressure reduced the overall complexity and stability of bacterial and fungal communities. Additionally, bacterial community assembly showed a tendency shift from stochastic to deterministic processes in response to increasing salinity, while fungal community assembly remained dominated by deterministic processes. provide robust evidence that soil salinity is a major inhibitor of soil biogeochemical processes in the Huinong District and plays a critical role in shaping bacterial and fungal communities, their symbiotic networks, and their assembly processes.

Soil is recognized as an important reservoir of antibiotic resistance genes (ARGs). However, the effect of salinity on the antibiotic resistome in saline soils remains largely misunderstood. In this study, high-throughput qPCR was used to investigate the impact of low-variable salinity levels on the occurrence, health risks, driving factors, and assembly processes of the antibiotic resistome. The results revealed 206 subtype ARGs across 10 categories, with medium-salinity soil exhibiting the highest abundance and number of ARGs. Among them, high-risk ARGs were enriched in medium-salinity soil. Further exploration showed that bacterial interaction favored the proliferation of ARGs. Meanwhile, functional genes related to reactive oxygen species production, membrane permeability, and adenosine triphosphate synthesis were upregulated by 6.9%, 2.9%, and 18.0%, respectively, at medium salinity compared to those at low salinity. With increasing salinity, the driver of ARGs in saline soils shifts from bacterial community to mobile gene elements, and energy supply contributed 28.2% to the ARGs at

Agricultural soils are important source and sink of antibiotic resistance genes (ARGs). However, little is known about the fate of ARGs in dryland soils, while its human exposure risks were seriously overlooked. Taking the northern Ningxia Plain as a case, this study explored the occurrence of ARGs and its relationship with mobile genetic elements (MGEs), pathogens, and environmental factors. Furthermore, the concentrations of airborne ARGs by soil wind erosion and the human exposure doses of soil ARGs were evaluated. The results showed the abundances of different regions ranged from 4.0 x 10(5) to 1.6 x 10(6) copies/g. Soil ARGs are driven by MGEs, but multiply impacted by soil properties, nutrition, and bacterial community. Vibrio metschnikovii, Acinetobacter schindleri, and Serratia marcescens are potential pathogenic hosts for ARGs. Further exploration revealed the concentration of ARGs loaded in dust by soil wind erosion reached more than 10(5) copies/m(3), which were even higher than those found in sewage treatment plants and hospitals. Skin contact is the primary route of ARGs exposure, with a maximum dose of 24071.33 copies/kg/d, which is largely attributed to ARGs loaded in dust. This study bridged the gap on ARGs in dryland soils, and provided reference for human exposure risk assessment of soil ARGs.

Promoting soil multifunctionality is pivotal for maintaining agricultural productivity and sustainable agriculture, especially with the increasing global population and food demand. The effectiveness of different agricultural practices in enhancing soil multifunctionality and how the combination can maximize soil multifunctionality remains unknown. This study aimed to investigate the different impacts of rotation (paddy-upland rotation and dryland rotation) combined with fertilization (chemical fertilizer and manure) on soil multifunctionality, microbial community structure, and microbial networks. A two-year field experiment was conducted at the Fengqiu National Agro-Ecosystem Observation and Research Station in Henan Province, China, comparing the differences between rice-wheat rotation (paddy-upland rotation) and maize-wheat rotation (dryland rotation) combined with chemical fertilizer and pig manure. This finding revealed that paddy-upland rotation combined with manure application had the optimal effect in enhancing soil multifunctionality with a 216.25 % enhancement while contributing to a yield increase of 222.71 %. Notably, paddy-upland rotation and fertilization significantly promoted the soil bacterial network complexity and robustness, and these network properties were crucial factors in predicting soil multifunctionality. This study provides a new insight for developing a comprehensive strategy combining paddy-upland rotation with manure application in the future, which can help us to better improve soil health and agricultural sustainability.

The municipal wastewater treatment systems (MWTSs) are the leading enrichment site of antibiotic resistance genes (ARGs), the occurrence of which in sewage and sludge significantly influences the ARGs burden of aerosols. However, the migration behavior and impact factors of ARGs in gas-liquid-solid phase are still unclear. This study collected gas (aerosol), liquid (sewage), and solid (sludge) samples from three MWTSs to explore the cross-media transport behavior of ARGs. The results showed that the main ARGs detected in the solid-gas-liquid phase were consistent, constituting the central antibiotic resistance system of MWTSs. Multidrug resistance genes dominated cross-media transmission (average relative abundance is 42.01 %). Aminocoumarin, fluoroquinolone, and aminoglycoside resistance genes (aerosolization index of 1.260, 1.329, and 1.609, respectively) were prone to migrating from the liquid to gas phase, resulting in long-distance transmission. Environmental factors (mainly temperature and wind speed), water quality index (mainly COD), and heavy metals may be the key factors affecting the trans-media migration of ARGs between the liquid, gas, and solid phase. Based on partial least squares path modeling (PLS-PM), the migration of ARGs in gas phase is primarily influenced by ARGs' aerosolization potential in liquid and solid phase, while heavy metals indirectly influences almost all categories of ARGs. Impact factors aggravated the migration of ARGs in MWTSs through co-selection pressure. This study clarified the key pathways and impact factors that form the cross-media migration behavior of ARGs, which can more specifically control ARGs pollution from different media.

Composting is a sustainable and eco-friendly technology that turns animal waste into organic fertilizers. It remains unclear whether differences exist in the structure of microbial communities during different livestock manure composting. This study analyzed the dynamic change of bacterial and fungal communities, metabolic function, and trophic mode during chicken manure (CM) and pig manure (PM) composting based on 16S rRNA and ITS sequencing. Environmental factors were investigated for their impact on microbial communities. During composting, bacterial diversity decreased and then increased, while fungal diversity slightly increased and then decreased. Saccharomonospora and Aspergillus were the dominant genera and key microorganisms in CM and PM, respectively, which played crucial roles in sustaining the stability of the ecological network structure in the microbial ecology and participating in metabolism. Saccharomonospora gradually increased, while Aspergillus increased at first and then decreased. PM had better microbial community stability and more keystone taxa than CM. In CM and PM, the primary function of bacterial communities was metabolism, while saprotroph was the primary trophic mode of fungal communities. Dissolved organic carbon (DOC) was the primary factor influencing the structure and function of microbial communities in CM and PM. In addition to DOC, pH and moisture were important factors affecting the fungal communities in CM and PM, respectively. These results show that the succession of bacteria and fungi in CM and PM proceeded in a similar pattern, but there are still some differences in the dominant genus and their responses to environmental factors.

IntroductionSoil microorganisms are essential for crop growth and production as part of soil health. However, our current knowledge of microbial communities in tobacco soils and their impact factors is limited.MethodsIn this study, we compared the characterization of bacterial and fungal communities in tobacco soils and their response to regional and rootstock disease differences.Results and discussionThe results showed that the diversity and composition of bacterial and fungal communities responded more strongly to regional differences than to rootstock diseases, while bacterial niche breadth was more sensitive than fungi to regional differences. Similarly, the core bacterial and fungal taxa shared by the three regions accounted for 21.73% and 20.62% of all OTUs, respectively, which was much lower than that shared by RD and NRD in each region, ranging from 44.87% to 62.14%. Meanwhile, the differences in topological characteristics, connectivity, and stability of microbial networks in different regions also verified the high responsiveness of microbial communities to regions. However, rootstock diseases had a more direct effect on fungal communities than regional differences.ConclusionThis provided insight into the interactions between microbial communities, regional differences, and rootstock diseases, with important implications for maintaining soil health and improving tobacco yield and quality.

Due to the massive use of antibiotics, antibiotics resistance genes (ARGs) are widely present in organic solid wastes (OSWs) such as livestock manure and sludge. As a common recycling technology, composting is often used to eliminate ARGs from OSWs. However, there is no bibliometric perspective on the fate of ARGs during OSWs composting to reveal the current state and future directions in this field. In this study, CiteSpace and VOSviewer were applied to count the available data from 2000 to 2021 in Web of Science to review the development and characteristics of relevant research. The results showed that the number of relevant papers and citations has increased consistently, with institutions and authors from China being the main contributors. The research belongs to the multidisciplinary with a focus on the environmental sciences ecology, and the results were mainly published in Bioresource Technology. Moreover, crucial literatures were screened to explore the framework within which the subject was constructed. Based on keyword analysis, the bacterial community was highlighted, and the research hotspots were revealed on the occurrence, affect factors including physicochemical properties, microbial community, MGEs, antibiotic, and heavy metal, and reduction strategies including exog-enous additive, microbial inoculant, and improved process, as well as the spread of ARGs in soil. This study provides the first review and summary of the hotspots, current challenges, and future directions of the fate of ARGs during OSWs composting based on bibliometric analysis, which could provide significant insights for effective control of the spread of ARGs from OSWs.

某城市污水厂预处理区、生化处理区以及污泥处理区三种不同区域产生的含恶臭和微生物气溶胶气体分别选择采用高效雾化喷淋技术、一体式高效集成除臭技术、高效雾化喷淋技术及一体式高效集成除臭结合的技术进行处理.结果表明,该城市污水厂不同功能区的含恶臭和微生物气溶胶气体取得较好的处理效果.

以城市污水厂格栅间为研究对象,在分析恶臭气体产生特性的基础上,研发出新型双介质气动雾化喷淋系统,并对系统运行参数进行了优化.将该系统应用于北京市某城市污水厂格栅间,考察了其对硫化氢和氨的去除效果.结果表明,该系统对污水厂格栅间的硫化氢和氨的去除率分别为90.54％～97.80％和93.75％～99.42％,取得了较好的除臭效果.该系统投资费用为95万元,运行费用为3.62万元/a,其中水费占运行费用的51.10％.新型双介质气动雾化喷淋系统较好适用于城市污水厂分散点源恶臭气体的原位处理.

A full-scale combined bioreactor with a biological aeration zone (BAZ) and a biological filtration zone (BFZ) was adopted to treat odors, volatile organic compounds (VOCs), and aerosols emitted from a landfill site. The BAZ mainly relies on suspended microorganisms that degrade substances with high water solubility, while the BFZ is divided into an acidic biofiltration unit (ABU) and a neutral biofiltration unit (NBU) to further remove remaining contaminants. Average removal efficiencies (REs) of 97.21%, 98.89%, 92.44%, 73.12%, and 81.89% for H2S, NH3, VOCs, culturable bacteria, and fungi, respectively, were achieved during the stable operation period. In particular, the concentrations of water-soluble ions (WSIs), including SO42−, NO2−, NO3−, and Cl− in aerosols, were significantly reduced by the combined bioreactor. The REs and degradation product characteristics for the odors, VOCs, and aerosols in the BAZ and BFZ were different due to different dominant microorganisms. In addition, the economic cost of the combined bioreactor was analyzed considering investment cost, operating cost, and packing material replacement cost, and a sensitivity analysis was performed to determine economically-sensitive parameters. This study clearly demonstrates the low cost and effectiveness of the combined bioreactor to treat exhaust gases containing odors, VOCs, and aerosols from a landfill site.

Two biofilters with low pH and neutral pH were operated on pilot scale for the treatment of complex gases containing hydrogen sulfide, ammonia, acetic acid, and toluene during 205 days. Under the coexistence of complex gases, the low-pH biofilter (LPB) had higher removal efficiency (RE) for hydrogen sulfide and toluene, and the maximum efficiencies were 99.24% and 99.90% respectively, while the neutral-pH biofilter (NPB) had higher REs of ammonia and acetic acid, up to 99.90% and 99.92% respectively. Higher pressure drop up to 622 Pa was achieved in the LPB, most likely caused by the special structure of fungi different from bacteria. Determination of the concentration of carbon-based intermediates revealed the dominant microbial removal of acetic acid and clarified the relationship between the generation of intermediate and the performance of biofilters. Respective amount of CO2 in the inlet and outlet showed that the mineralization capacity of the NPB was higher than that of the LPB, and it was more influenced by empty bed residence time (EBRT). The proportion of different forms of nitrogen and sulfur in the filler indicated that the removal of ammonia in the LPB mainly depended on the adsorption by moisture, while that in the NPB was microbial degradation, which was also the main removal pathway of sulfur regardless of pH condition. The removal and transformation of different substances in coexisting complex gases showed different characteristics in the LPB and NPB respectively.

Large amounts of bioaerosols are generated during the treatment of landfill leachate, which poses potential threat to public health safety. In this study, the concentrations, particle size distribution, and microbial community of bioaerosols emitted from the low-pH biofilter (LPB) and neutral-pH biofilter (NPB) used to treat the odors from landfill leachate, as well as the effect of the inlet flow rate (IFR) and water-containing rate (WCR) were investigated. The results showed that the removal efficiency of the LPB for heterotrophic bacteria and the NPB for fungi were higher when the IFR was higher. The outlet concentrations (OCs) of heterotrophic bacteria, neutral sulfur bacteria, ammonia-oxidizing bacteria, nitrite-oxidizing bacteria, NO2, and NO3 were negatively correlated with the IFR in the LPB, whereas those of fungi and acidophilic sulfur bacteria were positively correlated with the IFR. Inverse correlation between the OCs and IFR was exhibited in the NPB. The proportions of dominant microorganisms with large particle size (> 3.3 mu m) reduced significantly after both the LPB and NPB, which was aggravated under higher WCR. The analysis of microbial community illustrated that the dominant heterotrophic bacteria were different, while the dominant fungi were similar in the bioaerosols between the LPB and NPB, respectively. Due to the different emission characteristics, bioaerosols in the LPB and NPB outlet should be distinguished and taken seriously. (C) 2021 Elsevier Ltd. All rights reserved.

Biological control of odors and bioaerosols in wastewater treatment plants (WWTPs) have gained more attention in recent years. The simultaneous removal of odors, volatile organic compounds (VOCs) and bioaerosols in each unit of a full-scale integrated-reactor (FIR) in a sludge dewatering room was investigated. The average removal efficiencies (REs) of odors, VOCs and bioaerosols were recorded as 98.5 %, 94.7 % and 86.4 %, respectively, at an inlet flow rate of 5760 m3/h. The RE of each unit decreased, and the activated carbon adsorption zone (AZ) played a more important role as the inlet flow rate increased. The REs of hydrophilic compounds were higher than those of hydrophobic compounds. For bioaerosols, roughly 35 % of airborne heterotrophic bacteria (HB) was removed in the low-pH zone (LPZ) while over 30 % of total fungi (TF) was removed in the neutral-pH zone (NPZ). Most bioaerosols removed by the biofilter (BF) had a particle size larger than 4.7 μm while bioaerosols with small particle size were apt to be adsorbed by AZ. The microbial community in the BF changed significantly at different units. Health risks were found to be associated with H2S rather than with bioaerosols at the FIR outlet.