Andrographis paniculata, a medicinal species within the Acanthaceae family, is widely recognized as a "traditional Chinese herbal antibiotic," with andrographolides (ADs) serving as its primary bioactive components. Nevertheless, the molecular regulatory mechanisms governing the biosynthesis of ADs remain poorly understood. In this study, Ultraviolet-B (UV-B) radiation significantly induced the expression of ApIPPI, a gene encoding isopentenyl pyrophosphate isomerase, which is correlated with the accumulation of ADs. Sequence analysis revealed that the ApIPPI promoter contains a variety of MYB-binding elements. ApMYB9, a UV-B-responsive R2R3-MYB transcription factor belonging to the S20 subfamily, was identified by a yeast screening library. Subcellular localization and transcriptional activation assays demonstrated that ApMYB9 is localized in the nucleus and exhibits transcriptional activation capabilities. Integrated analyses using yeast one-hybrid (Y1H), dual-luciferase reporter (Dual-LUC) assays, and electrophoretic mobility shift assays (EMSA) confirmed that ApMYB9 targets specific MYB recognition motifs in the ApIPPI promoter to positively regulate its expression. Transient overexpression of ApMYB9 in A. paniculata leaves boosted ApIPPI transcript levels and promoted ADs biosynthesis. This study elucidates a UV-B response regulatory module in which ApMYB9 directly targets the ApIPPI promoter, redirecting metabolic flux to the accumulation of andrographolide. This provides a potential target for metabolic engineering aimed at optimizing the production of bioactive compounds from A. paniculata.

Andrographis paniculata is known for its diterpenoid medicinal compounds with antibacterial and anti-inflammatory properties. However, it faces production and cultivation challenges due to low temperatures (LTs). Cytochrome P450 monooxygenases (CYPs) are key enzymes in diterpenoid accumulation. Nevertheless, the functions and LT-related expression patterns of diterpenoid pathway CYPs in Andrographis paniculata remain poorly understood. In this study, 346 CYPs were discovered in Andrographis paniculata. Among them, 328 CYPs belonged to 42 known subfamilies. The remaining 17 CYPs might have represented novel subfamilies unique to this species. A total of 65 candidate CYPs associated with diterpenoid modification were identified. Of these, 50 were transmembrane proteins, and 57 were localized to chloroplasts. The CYP71 subfamily was the most abundant and had the highest motif diversity. Promoters of all candidate CYPs commonly contained elements responsive to gibberellins (GAs), methyl jasmonate (MeJA), and abiotic stresses. Notably, the XP_051152769 protein, corresponding to a CYP gene over 40,000 bp in length, featured an extraordinarily long intron (40,751 nts). Functional elements within this intron were related to LT, GAs, and dehydration pathways. Based on the promoter element arrangement and subfamily classification, 10 representative candidate CYPs were selected. Under LT stress, significant expression changes were observed in three representative CYPs: CYP71D, ent-kaurenoic acid oxidase (KAO), and ent-kaurene oxidase (KO). KAO and KO were significantly upregulated during early LT stress. KAO and KO interacted with each other and jointly interacted with GA20OX2-like. CYP71D acted as a negative response factor to LT stress. Among the 37 proteins interacting with CYP71D, 95% were CYPs. This study provides a critical preliminary foundation for investigating the functions of diterpenoid pathway CYPs in Andrographis paniculata, thereby facilitating the development of LT-tolerant cultivars.

Andrographolide is the main active ingredient of Andrographis paniculata, which has the functions of clearing heat and detoxifying, antibacterial and anti-inflammatory, protecting liver and improving gallbladder. It is clinically used to treat pneumonia in children and upper respiratory tract infection. In recent years, because of the influence of environmental factors for a long time, not high purity and good varieties of selective breeding work lag and so on reasons, greatly restricted the quality improvement of andrographolide. In the quality evaluation of A. paniculata, the content of andrographolide is a very important index. Analyzing the molecular mechanism and accumulation rule of andrographolide biosynthesis is helpful to improve the quality of A. paniculata. In this review, the biosynthetic pathways and regulatory molecular mechanisms of andrographolide are comprehensively discussed. The biosynthesis of andrographolide is mainly divided into two stages, including the synthesis stage of andrographolide precursor and the modification stage of andrographolide synthesis. The synthesis of precursor has been relatively clear, but the mining of the pathways and key enzymes in the modification stage of andrographolide synthesis is still at a blank, and further research is needed. Through the excavation of the key factors affecting the synthesis and accumulation of andrographolide from the two levels of environmental factors and cropping systems, it is conducive to the establishment of a perfect and high-quality A. paniculata cultivation system. In addition, the research progress on the regulation of andrographolide biosynthesis was summarized and prospected, in order to provide research reference for the improvement of A. paniculata quality.

BACKGROUND:The R2R3-MYB transcription factors are a crucial and extensive gene family in plants, which participate in diverse processes, including development, metabolism, defense, differentiation, and stress response. In the Lingnan region of China, Morinda officinalis is extensively grown and is renowned for its use as both a medicinal herb and food source. However, there are relatively few reports on the R2R3-MYB transcription factor family in M.officinalis.RESULTS:In this study, we identified 97 R2R3-MYB genes in the genome of Morinda officinalis and classified them into 32 subgroups based on phylogenetic comparison with Arabidopsis thaliana. The lack of recent whole-genome duplication events in M.officinalis may be the reason for the relatively few members of the R2R3-MYB family. We also further analyzed the physical and chemical characteristics, conserved motifs, gene structure, and chromosomal location. Gene duplication events found 21 fragment duplication pairs and five tandem duplication event R2R3-MYB genes in M.officinalis may also affect gene family expansion. Based on phylogenetic analysis, cis-element analysis, co-expression analysis and RT-qPCR, we concluded that MoMYB33 might modulate flavonol levels by regulating the expression of 4-coumarate-CoA ligase Mo4CL2, chalcone isomerase MoCHI3, and flavonol synthase MoFLS4/11/12. MoMYB33 and AtMYB111 showed the highest similarity of 79% and may be involved in flavonol synthase networks by the STRING database. Moreover, we also identified MoMYB genes that respond to methyl Jasmonate (MeJA) and abscisic acid (ABA) stress by RT-qPCR.CONCLUSIONS:This study offers a thorough comprehension of R2R3-MYB in M.officinalis, which lays the foundation for the regulation of flavonol synthesis and the response of MoMYB genes to phytohormones in M.officinalis.

Medicinal plant microRNAs (miRNAs) are an endogenous class of small RNA central to the posttranscriptional regulation of gene expression. Biosynthetic research has shown that the mature miRNAs in medicinal plants can be produced from either the standard messenger RNA splicing mechanism or the pre-ribosomal RNA splicing process. The medicinal plant miRNA function is separated into two levels: (1) the cross-kingdom level, which is the regulation of disease-related genes in animal cells by oral intake, and (2) the intra-kingdom level, which is the participation of metabolism, development, and stress adaptation in homologous or heterologous plants. Increasing research continues to enrich the biosynthesis and function of medicinal plant miRNAs. In this review, peer-reviewed papers on medicinal plant miRNAs published on the Web of Science were discussed, covering a total of 78 species. The feasibility of the emerging role of medicinal plant miRNAs in regulating animal gene function was critically evaluated. Staged progress in intra-kingdom miRNA research has only been found in a few medicinal plants, which may be mainly inhibited by their long growth cycle, high demand for growth environment, immature genetic transformation, and difficult RNA extraction. The present review clarifies the research significance, opportunities, and challenges of medicinal plant miRNAs in drug development and agricultural production. The discussion of the latest results furthers the understanding of medicinal plant miRNAs and helps the rational design of the corresponding miRNA/target genes functional modules.

[目的]对紫外线C(UV-C)处理的穿心莲叶片进行转录组测序分析,挖掘穿心莲内酯合成相关基因,为深入探究穿心莲内酯合成相关基因的调控机制和生物学功能提供理论参考.[方法]选用生长60 d的穿心莲幼苗为材料,经UV-C照射2 h后,利用Illumina HiSeq二代测序平台进行转录组测序,并结合生物信息学软件和实时荧光定量PCR(qRT-PCR)对所得序列进行功能注释和相对表达水平验证,挖掘响应UV-C的穿心莲内酯合成相关基因.[结果]共注释到21545个穿心莲基因,有2137个基因呈显著差异表达模式,其中,1147个基因显著上调表达,990个基因显著下调表达.GO功能注释结果显示,UV-C处理造成了氧化胁迫,线粒体氧化磷酸化电子传递链的NADH脱氢酶基因上调表达.KEGG代谢通路富集结果显示,二萜类物质穿心莲内酯的前体(E,E,E)-香叶基香叶基二磷酸(GGPP)合成途径[甲羟戊酸(MVA)途径和2-C-甲基-D-赤藓糖醇-4-磷酸(MEP)途径]差异表达基因显著富集.转录组测序结果和实时荧光定量PCR(qRT-PCR)检测结果均显示,MVA途径的4种合成酶基因HMGS(CXN00016849)、HMGR(CXN00000058)、MVK(CXN00002900)和MVD(CXN00004398)及GGPP合成关键酶基因FPS的表达量显著上调;MEP途径中,仅DXS(CXN00013302)的表达量显著下调.蛋白互作预测结果显示,2个途径中的差异表达基因编码蛋白之间存在互作,HMGS与CYP71家族成员之间,以及FPS1与UGT71、UGT73和UGT76之间的互作网络丰富.[结论]UV-C照射调控穿心莲重要生命进程,其中,次生代谢、氧化磷酸化、光合作用等途径基因转录本的相对表达量呈UV-C特异性响应模式.合成穿心莲内酯前体GGPP的细胞质MVA途径基因显著上调表达,推测UV-C诱导穿心莲内酯合成的主要场所为细胞质.穿心莲MVA途径基因及CYP71、UGT71、UGT73和UGT76可作为穿心莲内酯合成途径研究的候选分子靶标.

Ultrastructural changes in cerebral tissue subjected to temporary occlusion on the right middle cerebral artery for intervals of 30 min, 1, 2, 3, and 4 hr were studied after daily injections of 10% glycerol in saline. These changes were compared with previously reported data from untreated animals. The extent of tissue involvement was appreciably less in the glycerol-treated group as evidenced by the reduction of intracellular edema and fewer changes in the membranes of ultrastructural elements (mitochondria, endoplasmic reticulum, Golgi complex, and plasmalemma) known to be essential for cellular metabolism and function in the central nervous system.

目的:筛选合适的穿心莲茉莉酸甲酯(MeJA)和非生物胁迫下实时荧光定量聚合酶链式反应(Real-time PCR)检测的内参基因.方法:利用高温、干旱、紫外和MeJA处理的穿心莲转录组数据,挑选到7个候选内参基因肌动蛋白1(ACT1),肌动蛋白2(ACT2),转录延伸因子(EF-1α),甘油醛-3-磷酸脱氢酶(GAPDH),微管蛋白(TUB),多聚泛素酶(UBQ)和18S核糖体RNA (18S),并以上述处理后的穿心莲叶片为实验材料,进行Real-time PCR验证.利用geNorm,NormFinder和BestKeeper 3种内参稳定性评估软件进行分析,再利用Refinder软件进行综合分析.结果:3种软件基于不同指标进行稳定性评估,结果并不相同,综合分析得出候选内参基因在高温胁迫下表达稳定性顺序依次为UBQ＞ 18S＞EF-1d＞A CT2＞A CT1 ＞GAPDH＞ TUB;干旱胁迫下表达稳定性顺序依次为A CT1＞ UBQ＞EF-1 α＞ 18S＞A CT2＞GAPDH＞TUB;UV胁迫下表达稳定性顺序依次为EF-1α＞TUB＞A CT2＞UBQ＞18S＞GAPDH＞ACT1 ;MeJA胁迫下表达稳定性顺序依次为ACT1 ＞EF-1 α＞ UBQ＞A CT2＞ 18S＞TUB＞GAPDH.其中18S基因表达丰度较高,不适合作为内参基因.结合转录组数据对4种胁迫中穿心莲内酯合成相关基因酶羟甲基戊二酰-辅酶A合成酶(HMGS)的相对表达水平验证,发现合适内参基因的Real-time PCR结果更可靠.结论:穿心莲高温、干旱、紫外和MeJA胁迫时,UBQ,ACT1和UBQ,EF-1α和TUB,ACT1和EF-1α分别为最适内参基因组合,为后期开展穿心莲高温、干旱、紫外和MeJA处理中基因的功能调控和表达研究提供了参考.

The plant growth, development, and secondary metabolism are regulated by R2 R3-MYB transcription factors. This study identified the R2 R3-MYB genes in the genome of Andrographis paniculata and analyzed the chromosomal localization, gene structure, and conserved domains, phylogenetic relationship, and promoter cis-acting elements of these R2 R3-MYB genes. Moreover, the gene expression profiles of R2 R3-MYB genes under abiotic stress and hormone treatments were generated by RNA-seq and validated by qRT-PCR. The results showed that A. paniculata contained 73 R2 R3-MYB genes on 21 chromosomes. These members belonged to 34 subfamilies, 19 of which could be classified into the known subfamilies in Arabidopsis thaliana. The 73 R2 R3-MYB members included 36 acidic proteins and 37 basic proteins, with the lengths of 148-887 aa. The domains, motifs, and gene structures of R2 R3-MYBs in A. paniculata were conserved. The promoter regions of these genes contains a variety of cis-acting elements related to the responses to environmental factors and plant hormones including light, ABA, MeJA, and drought. Based on the similarity of functions of R2 R3-MYBs in the same subfamily and the transcription profiles, ApMYB13/21/35/67/73(S22) may regulate drought stress through ABA pathway; ApMYB20(S11) and ApMYB55(S2) may play a role in the response of A. paniculata to high temperature and UV-C stress; ApMYB5(S7) and ApMYB33(S20) may affect the accumulation of andrographolide by regulating the expression of key enzymes in the MEP pathway. This study provides theoretical reference for further research on the functions of R2 R3-MYB genes in A. paniculata and breeding of A. paniculata varieties with high andrographolide content.

广藿香为多年生芳香草本或半灌木植物,是我国著名的南药之一.通常以其干燥地上部分入药,具有芳香化浊、开胃止呕、发表解暑等功效,是多种中成药的重要原料.此外,以其提取的广藿香油是世界上医药工业和轻化工业的重要原料,经济价值高,应用市场前景广阔.广藿香为热带地区引入我国栽培,种质类型单一,遗传基础狭窄,资源极为有限.由于长期的自然环境和人工栽培的影响,广藿香种质产生了一些变异和分化,目前主要分布于我国广东、广西、福建和海南等南方省(区),按照产地将其分为牌香、肇香、湛香和南香4种.广藿香在种植过程中,存在严重的连作障碍现象,且由于田间管理粗放,其产量和品质不稳定,受品种、产地、栽培技术等多方面因素影响.近年来,随着分子生药学的发展,其主要药用成分广藿香醇和广藿香酮的内在分子调控机制相关研究开展顺利,目前关于广藿香的研究大多集中在其有效成分的药效药理、生物合成及代谢调控方面,而对种质资源及栽培技术的系统性研究不足.优良的品种和高效规范的种植技术是药材产量和质量稳定的基础,为充分利用广藿香药材资源,进一步推广广藿香规模化和产业化发展,本文主要综述了广藿香的生物学特性、种质资源研究现状、栽培技术中的瓶颈及产业发展过程中存在的问题,指出了广藿香栽培过程中养分管理技术缺乏,连作障碍问题严重,提出要加强广藿香的繁殖技术和品种选育研究,积极推进广藿香的规范化种植,以期为广藿香的产业发展和开发利用提供参考.

为了解穿心莲(Andrographis paniculata)的研究现状和学科发展,通过科学网(Web of Science,WoS)核心合集搜索2001-2020年发表的穿心莲SCI学术论文,结合文献计量学描述性和关联性指标,明确穿心莲近20年的科研态势.结果表明:(1)2019-2020年穿心莲的年发文量突破100篇,多数以穿心莲内酯的抑癌功能为核心展开.(2)虽然发文量最多的国家是印度、中国和马来西亚,但中国与美国和澳大利亚的合著关系最近.结合发文量、H指数与论文年均被引数,美国仅凭中国发文量1/5的产出就达到与中国持平的国际影响力,成果受到高度重视,中国穿心莲论文质量仍需提高.(3)印度科学工业研究理事会(CSIR)和新加坡国立大学是被引频次最高的穿心莲科研机构.台湾中华医科大学、中国科学院、上海中医药大学和中山医学大学是中国主要的穿心莲科研机构.(4)穿心莲发文量前3名作者均来自中国.(5)新加坡国立大学Wong W S F团队于2009年发表穿心莲至今为止影响因子最高(21.4)的学术论文,阐明穿心莲内酯缓解哮喘的分子机制."天然抗生素"穿心莲内酯抑制新型冠状病毒蛋白活性的功能公开引起国际学者的高度重视,关于穿心莲药理药效和高产稳产等基础研究将更加深入.

为促进植物环状RNA(Circular RNAs)的研究进程,利用文献综述法检索近三年(2018-2020)发表的环状RNA文献,从功能发挥、研究手段及应用前景等方面分别对动物和植物环状RNA的研究进展进行整理讨论.结果表明:环状RNA可作为竞争性内源RNA与microRNAs(MiRNAs)特异性结合,间接调控蛋白表达水平.其还可调节来源基因的转录效率、滚环式翻译多肽及作为蛋白支架调控细胞进程.截止目前,临床有关环状RNA的研究最为深入;经过潜在诊断、预断和治疗方法评估后,环状RNA有望成为诊疗疾病的生物标志物.而植物环状RNA在不同生长发育阶段、激素刺激及逆境胁迫等条件下也展现出丰富的差异表达模式,初步体现其功能的多样性.但与动物环状RNA的研究深度相比,植物环状RNA研究还处于兴起阶段,与植物环状RNA相关的数据库和分析工具还有待开发.综上,本文重点评述动植物体内不同类型环状RNA在生成机制、剪切识别位点、功能发挥及应用潜力上的特异性和同源性,讨论动植物环状RNA研究存在的主要问题并对深入开展植物环状RNA研究进行展望.

[目的]利用高通量测序技术解析红脚艾(Artemisia rubripes Nakai)的转录组信息特征.[方法]通过高通量测序平台Illumina HiSeq 2500对红脚艾进行转录组测序,通过Trinity软件de novo组装获得Unigene,并基于序列同源性对Unigene进行功能注释,得到红脚艾的转录组信息.[结果]测序数据经过质控后共获得24126043条高质量的reads,通过de novo组装获得173093个转录本,对组装的转录本去冗余后共获得85991个Unigene,平均长度为616.87 bp,N50为925 bp.共有47216个Unigene在NR、KEGG、COG、KOG、GO数据库获得功能注释,40802个Unigene在NR数据库注释,显示红脚艾与向日葵(Helianthus annuus)的单基因匹配率最高,16846个Unigene被KEGG数据库注释到130条代谢途径中,26171个Unigene被注释到25个KOG功能分类中,23203个Unigene被GO注释到生物过程、细胞组成和分子功能三大类51个功能分类,12810个Unigene被注释到25个COG功能分类中.[结论]利用高通量测序技术获得了红脚艾转录组信息特征,这些数据将为后期开展功能基因鉴定、解析化合物次生代谢途径及其调控机制奠定研究基础.

Plant roots absorb K+ from soil via K+ channels and transporters, which are important for stress responses. In this research, GmAKT1, an AKT1-type K+ channel, was isolated and characterized. The expression of GmAKT1 was induced by K+-starvation and salinity stresses, and it was preferentially expressed in the soybean roots. And GmAKT1 was located in the plasma membrane. As an inward K+ channel, GmAKT1 participated in K+ uptake, as well as rescued the low-K+-sensitive phenotype of the yeast mutant and Arabidopsis akt1 mutant. Overexpression of GmAKT1 significantly improved the growth of plants and increased K+ concentration, leading to lower Na+/K+ ratios in transgenic Arabidopsis and chimeric soybean plants with transgenic hairy roots. In addition, GmAKT1 overexpression resulted in significant upregulation of these ion uptake-related genes, including GmSKOR, GmsSOS1, GmHKT1, and GmNHX1. Our findings suggested that GmAKT1 plays an important part in K+ uptake under low-K+ condition, and could maintain Na+/K+ homeostasis under salt stress in Arabidopsis and soybean plants.

中草药是中医药产业可持续发展的物质基础,对推动社会经济的全面发展具有重要意义.优良种苗是中草药标准化生产和高效种植的基础,也是中药农业的重要组成部分.在中草药农业生产上,病原菌或病毒的侵染是导致中药材产量和品质降低的主要原因之一.特别是无性繁殖的中草药品种,在自然环境中长期经受各种病菌或病毒的重复传染而影响光合作用效率导致种苗的生长势变弱,种性退化,种植效益降低.而使用植物脱毒技术培育的种苗可有效避免病菌或病毒的传播积累,获得健康种苗,具有重要应用价值和商业前景.植物脱毒技术是一项现代生物技术,大多是基于植物组织培养技术,植物脱毒技术的利用,促进了果树、作物良种的推广,给农业带来一场新的技术革命.利用植物脱毒技术培育健康中草药种苗,达到提纯复壮,增强中草药种苗的抗性,也促进中药材种业的发展.综述了植物脱毒的技术方法及其在中草药种苗上的研究进展与应用现状,同时对利用植物脱毒技术开展中草药健康种苗的标准化生产进行探讨,以期为中草药种业的持续发展提供参考.

为从转录水平上解析穿心莲体内主要进行的生物进程及其分子机制,选取生长60天的福建漳州生产用穿心莲苗为材料,利用超长单分子测序技术测得其根、茎和倒三叶的全长转录组初始序列信息.对初始序列进行筛选、去冗余、校正、功能注释和基因结构分析.结果 显示,穿心莲基因的功能主要富集于代谢途径、次生代谢产物合成途径及抗生素合成途径.bHLH、bZIP、MYB和WRKY等直接参与次生代谢的转录因子含量位居前10.穿心莲内酯前体合成途径基因出现可变剪切,主要为内含子保留.其中,有1个基因产生了6个可变启动子式的内含子保留mRNA亚型.总之,次生代谢是穿心莲的主要生物活性进程,相关功能基因可通过结合丰富的转录因子和进行高频的可变剪切参与其中.

•Circular RNAs were identified in a whole genome in soybean.•The existence of circular RNAs were validated in cold-stressed soybean.•Circular RNAs were predicted to be involved in low temperature responses.•Circular RNAs were found to act as the sponges for miRNAs in soybean.•Circular RNAs were found to have protein-coding potential in soybean.

Cold stress is a major abiotic factor that inhibits soybean ( Glycine max (Linn.) Merr.) yield. MicroRNAs (miRNAs) are prevalent endogenous gene regulators in eukaryotes that can cleave target messenger RNAs and play essential roles in soybean cold response. In this study, 105 genes, including 22 pentatricopeptide repeat ( PPR ) genes and 4 growth-related genes, were predicted to be the target genes of soybean miRNA miR1508a ( gma-miR1508a ). The expression of gma-miR1508a was induced by cold treatment but inhibited by drought stress. Compared to the wild type (WT), the overexpression of gma-miR1508a in soybean plants resulted in dwarfing and thick cell walls. Gma-miR1508a transgenic plants exhibited cold tolerance at the germination and young seedling stages, and soluble sugar content was 58.7% higher than that of WT plants under cold stress. The gma-miR1508a transgenic lines showed lower survival rates and greater leaf water loss compared to the WT under drought treatment, which indicated that gma-miR1508a reduced drought resistance in soybean. Transcript abundances of 2 PPR and 4 growth-related predicted target genes decreased in gma-miR1508a transgenic plants. In the above six genes, the expression patterns of Glyma.16G162100 ( PPR ) and Glyma.17G065400 (xyloglucan endo-trans-glucosylase/hydrolase, XTH ) showed opposite trends with gma-miR1508a under cold conditions. 5′-Rapid amplification of complementary DNA ends (5′-RACE) assays of Glyma.16G162100 revealed that the cleavage sites of gma-miR1508a are located at the 15th and 21st nucleotides of the complementary region. This study demonstrated that gma-miR1508a confers dwarfing, cold tolerance, and drought sensitivity in soybean.

Acanthopanax trifoliatus (Linn.) Merr.is an edible vegetables and medicinal plant from Asian countries. In this study, the complete chloroplast genome ofA. trifoliatuswas assembled and annotated by high-throughput sequencing. The total chloroplast genome size ofA. trifoliatuswas 156,716 bp, containing a large single-copy (LSC) region of 86,672 bp, a small single-copy (SSC) region of 18,174 bp, and a pair of inverted repeat regions of 25,935 bp. A total of 134 genes were predicted in the chloroplast genome ofA. trifoliatus, including 89 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Phylogenetic analysis showed thatA. trifoliatuswas closely related toEleutherococcus gracilistylus.

植物液泡铁离子转运蛋白可维持细胞内铁离子含量,保证细胞正常生长,在植株抵抗盐碱环境过程中发挥重要作用.在大豆中克隆得到液泡铁离子转运蛋白同源基因GmVIT1,蛋白结构域分析结果表明,GmVIT1属于CCC1蛋白超家族,具有高度保守的结构域.研究发现GmVIT1具有跨膜结构,并利用YFP荧光蛋白确定GmVIT1蛋白定位在液泡膜上;实时荧光定量PCR(qRT-PCR)显示,GmVIT1在大豆根、茎、叶、花、荚和种子中均表达,花中mRNA丰度相对较高;胁迫后发现,GmVIT1的mRNA积累受ABA负调控,盐胁迫下基因显著上调表达.大豆毛状根试验发现,过量表达GmVIT1的毛状根耐受150 mmol·L-1氯化钠;转基因拟南芥耐盐性试验表明GmVIT1可显著提高植物耐盐能力.