Objective:To investigate the effect of Manchu medicine north-Schisandra chinensis polysaccharide (NSCP) on the human neutrophils treated by lipopolysaccharide (LPS) cultivated in vitro,and to elucidate its anti inflammatory mechanism.Methods:The neutrophilic inflammatory cell model was established with LPS.The experiment included control group,LPS group (1.0 mg · L-1) and NSCP group (1.25,2.50 and 5.00 g · L-1),the cells in NSCP group were first treated with LPS for 60 min,and then treated with different concentrations of NSCP.The levels of TNF-α in neutrophils were measured with ELISA and the apoptotic rates were detected by flow cytometry.Results:The level of TNF-a in LPS group was increased compared with control group (P＜0.05).The level of TNF-α in NSCP group was decreased compared with LPS group (P＜0.05).The apoptotic rate in LPS group was decreased compared with control group (P＜0.05);the apoptotic rates in NSCP group were increased with the increasing of time and dose,and the best effect was found 16 h after treatment with 5 g · L-1NSCP:the apoptotic rate in NSCP group was significantly increased compared with LPS group (P＜0.05).Conclusion:NSCP can perform the anti-inflammation effect through the suppression of LPS-induced TNF-α secretion in neutrophils and the promotion of neutrophils apoptosis.

中性粒细胞( Polymorphonuclear neutrophil , PMN)是介导炎症反应的重要效应细胞,在机体非特异性免疫防御中起重要作用. 正常生理情况下PMN的增殖与凋亡保持动态平衡,既有利于机体的防御反应,又有利于炎性反应的消散. 但在严重创伤、脓毒血症、烧伤、缺血再灌注等条件下, PMN 过度激活、聚集于靶组织、释放大量毒性介质及产生大量的炎性细胞因子,超过防御需要、持续过度的炎性反应会造成正常组织细胞的损伤. PMN的数量、功能及存活时间会影响炎症反应的发生、发展过程及转归. 适时抑制PMN过强的效应或促进其凋亡则有利于减轻机体损伤. 目前,以PMN过度浸润为特征、免疫系统控制失调产生的非特异性炎症,与哮喘、类风湿性关节炎、溃疡性结肠炎、急性肺损伤、牙周炎、结核病及急性心肌梗死等多种疾病有关. 因此了解PMN凋亡机制,适度地调控PMN凋亡,对于治疗创伤、控制炎症具有重要意义. 本文就中性粒细胞凋亡及凋亡抑制在炎症中的作用等进行综述.

目的 探讨北五味子多糖对脂多糖(LPS)处理的中性粒细胞炎性因子白细胞介素(IL)-6含量及细胞凋亡的影响.方法 实验分为空白对照组、LPS组及北五味子多糖组,使用酶联免疫吸附法(ELISA) 检测LPS处理的中性粒细胞的IL-6含量及原位末端标记法(TUNEL)检测凋亡.结果 北五味子多糖可以抑制IL-6的分泌,随浓度的增加抑制作用增强,与LPS组相比,随时间延长抑制作用增强,16 h抑制作用最好,与LPS组相比IL-6的含量显著降低(P<0.05).LPS可使中性粒细胞凋亡延迟,作用16 h,LPS组凋亡指数与对照组相比显著降低(P<0.05);北五味子多糖组凋亡指数随时间延长和浓度增加而增加,5 mg/ml北五味子多糖作用16 h促凋亡作用最好,与LPS组相比显著升高(P<0.05).结论 北五味子多糖可减少LPS处理的中性粒细胞IL-6的含量,并可促进中性粒细胞凋亡.

当事人常以逃避刑事责任,或为出气解恨,或反诬他人,或为达到赔偿或恶意索赔为目的,会有意夸大伤情,甚至伪造伤情是造作上的形成原因,但造作伤程度一般是刚超过轻伤下限.造作伤的常见类型包括伪造外伤性鼓膜穿孔、手术或其它锐利器具扩创、伪造牙齿外伤性脱落、伪造难免流产、伪造血尿、功能方面的问题和其他情况等,这要求办案人员做到公平、公正,保证伤者实现正当诉求;办案人员应迅速接触伤者,了解损伤情况,制作询问笔录,尽量不给其可乘之机;法医深入到医院现场,在第一时间检验伤者所受到的损伤;应加强法制教育,对既往的造作伤涉案人员依法严惩公开曝光,从而警示大多数人;加强医疗监督,防止篡改、修改、销毁医学等情况的发生.在法医鉴定过程中,要详细了解案情,认真进行现场勘查,全面细致检验损伤,仔细检查衣物,客观分析病例资料,维护法医鉴定的科学客观公正性.

To make a comprehensive summary of Glycyrrhiza uralensis Fisch.on its antineoplastic function, functional mechanism and clinical application, to provide a theoretical basis in order to seek natural antineoplastic.