Antioxidative Effects of Each Fraction of Buckwheat Sprout Grown under Different Visible Light 1) Myojung Kim, 1)EunSong Kim, 2)MoonSik, Jang, 2)Uk‐Sun Jung 3)SeokHyun Eom, 1)Bomo Lee, 1)Soip Park, 1)Chaewon Jo, 1)Myunghwa Kang 1) 3Department of Food Science & Nutrition/Institute of Basic Science, Hoseo University, Asan, Korea, Republic 2)Natural Solution Co., Ltd, Suwon, Korea, Republic 3) Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, Korea, Republic In this study, buckwheat sprouts were grown under blue light, red light, dark room and fluorescent light in plant company. Their were subjested to different drying processes as freeze drying and hot air drying. Each solvent fractions has studied total polyphenol acid contents, flavonoids contents, DPPH, ABTS radical scavenging activities, SOD‐liked activities, reducing power measurement and hydroxyl radical scavenging activity. Total phenolic acid contents were 3.07 ± 0.0355mg/ml in FDR(freeze drying red light) butanol fraction layer, 3.05mg/ml in FDR ethyl acetate layer and 2.93 ± 0.1000mg/ml in HDD(hot air dry dark light) ethyl acetate layer. DPPH scavenger activity were 84.15% in FDR butanol layer, 82.94% in HDF ethyl acetate layer and 81.09% in FDF(freeze drying white) butanol layer. SOD ‐like activity were 91.53 ± 0.4088mg/ml in HDF ethyl acetate layer, 91.29 ± 0.000mg/ml in HDD ethyl acetate layer and 88.55 ± 0.1665mg/ml in HDD chloroform layer. ABTS activity were FLB 94.14 ± 0.3872mg/ml, 93.98 ± 0.0877 mg / ml in HDR hexane layer and 93.98 ± 0.7896 mg / ml in FDF hexane layer. Total flavonoid content were 5.17 ± 0.2276% in butanol layer FDR, 4.82 ± 0.0710% in FDR ethyl acetate layer and 4.79 ± 0.1049% in HDB butanol layer, respectively.Grant Funding Source: No

Safflower sprouts were grown under different light frequencies and conditions, including fluorescent white light, red LED or blue LED light and darkness. The sprout extracts were tested for their inhibitory effect on melanin synthesis. Two compounds from safflower sprout extract were isolated and tested for their activities. Safflower seeds were sown in growth chambers set to lights and harvested at 9 days after sowing. Methanolic extracts of safflower sprouts were subjected to melanin synthesis inhibitory effect on MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetraxolium Bromide] and melanin bioassay methods with a dose dependent manner. Two compounds were identified by using open column chromatography and LC-MS (liquid chromatography-mass spectrometry) analysis. The MTT and melanin bioassays with the identified compounds were conducted. Darkness or fluorescent white light irradiation induced extracts with inhibitory effects on melanin synthesis (in a dose dependent manner). In contrast, blue light induced extracts to slightly increase melanin synthesis. It was found that 8-hydroxyarctigenin, a compound isolated from the safflower sprouts grown under fluorescent white light for 9 days after germination, induced inhibitory effect of melanin synthesis. The compound was contained more in the darkness or fluorescent white light treatment than others. Safflower sprouts grown under dark or fluorescent white light conditions were more effective on inhibiting melanin synthesis than conditions under red or blue light. An active compound, 8-hydroxyarctigenin, may be a useful whitening agent in cosmetic resources.

홍화새싹을 적색, 푸른색, 형광등과 대조군의 조명하에서 재배한 홍화새싹의 전처리 방법을 개발하기 위하여 동결 및 열풍건조 후 각종 이화학적인 특성을 분석하였다. 열풍건조 후 홍화새싹의 수분함량은 형광광원 9.38%, 푸른광원 5.70, 적색광원 12.21 및 빛이 없이 자란 대조군 7.87%로 나타나 수분 함량은 적색광원이 가장 높았다. 한편 동결건조 후 수분 함량은 형광광원 9.95%, 푸른 광원 11.30%, 적색광원 11.25% 및 대조군 10.45%로 나타나 수분 함량은 적색광원과 푸른 광원에서 비슷한 수준이었다. 조회분 함량 분석결과 열풍건조한 홍화새싹은 형광광원 4.82%, 푸른광원 12.22%, 적색광원 4.33% 및 대조군 6.30%였고 동결건조 후에는 형광광원 4.75%, 푸른광원 4.44, 적색광원 4.00 및 대조군 3.65%로 나타났다. 열풍건조한 홍화새싹의 조지방 함량 분석결과 형광광원 16.52%, 푸른광원 15.40%, 적색광원 14.55% 및 대조군 15.46%였고 동결건조 후에는 형광광원 25.19%, 푸른광원 15.72%, 적색광원 25.53% 및 대조군 20.13%로 나타났다. 클로로필 함량 분석결과 열풍건조보다 동결건조로 전처리 시 또한 재배광원에 따라 영양성분이 다르게 분포하는 것으로 나타났다. 무기질 함량 분석결과 건조방법 및 재배 광원에 관계없이 K, Ca, P 및 Mg의 함량이 매우 높아 우수한 알카리성 식품으로 추천할 수 있겠다. Physicochemical properties of safflower sprouts grown in there different lights red, blue, and white; control exposed to limited light and subjested to different drying processes were investigated. Moisture contents of safflower sprouts grown different lights and treated with hot dry air were as follows: 9.38%(white light), 5.70%(blue light), 12.21%(red light), and 7.84%(control; no light). The moisture contents of freeze-dried safflower sprouts were as follow as: 9.95%(white light), 11.30%(blue light), 11.25%(red light), and 10.45%(control). Crude ash contents of the hot air-dried sprouts were as follows: 4.82%(white light), 12.22%(blue light), 4.33%(red light), and 6.30%(control). The crude ash contents of freeze-dried safflower sprouts were as follow as: 4.75%(white light), 4.44%(blue light), 4.00%(red light), and 3.65% (control). Crude protein contents of hot air-dried safflower sprouts were as follows: 16.52%(white light) 15.40%(blue light), 14.55%(red light), and 15.46%(control). The crude ash contents of freeze-dried safflower sprouts were as follows: 25.19%(white light), 15.72%(blue light), 25.53%(red light), and 20.13%(control). Chlorophyll contents of hot air-dried and freeze-dried safflower sprouts were higher than those of only freeze-dried sprouts. b-values of freeze-dried sprouts were lower than those of sprouts dried by other dried methods. The contents of minerals Ca, Mg, Na, P and K in all the sprout samples remains unchanged regardless of the drying methods.