Background:The role of 4-hydroxyphenylpyruvate dioxygenase-like protein (HPDL) in endometrial cancer (EC) progression remains poorly understood, particularly its involvement in metabolic-epigenetic crosstalk via lactate-driven histone lactylation. This study aimed to investigate HPDL's mechanistic contribution to EC pathogenesis. Methods:Stable HPDL-overexpressing and knockdown EC cell lines (HEC-1-B and AN3CA) were generated using lentiviral vectors. Functional assays (proliferation, migration, invasion), subcutaneous xenograft models in BALB/c nude mice, and molecular analyses were conducted. Lactate levels, Pan-lysine lactylation (pan-kla), histone H3K18 lactylation (H3K18la), and effects of sodium oxamate (lactate modulator) were assessed. Lactate Dehydrogenase A/Lactate Dehydrogenase B (LDHA/LDHB) knockdown, promoter activity assays, and chromatin immunoprecipitation (ChIP) were performed to evaluate H3K18la occupancy at LDHA/LDHB promoters. Results:HPDL knockdown reduced intracellular lactate, Pan-Kla, and H3K18la levels, while overexpression elevated these markers. Sodium oxamate amplified lactate and lactylation in HPDL-overexpressing cells but suppressed histone lactylation independently of HPDL. LDHA/LDHB knockdown diminished lactylation, repressed HPDL expression, and inhibited promoter activity. ChIP revealed H3K18la enrichment at LDHA/LDHB promoters in HPDL-overexpressing cells and reduced occupancy in knockdown models. HPDL enhanced EC cell proliferation, migration, and invasion in vitro. In vivo, HPDL-overexpressing xenografts exhibited accelerated tumor growth and larger volumes compared to controls. Conclusions:HPDL regulates histone lactylation via LDHA/LDHB and promotes the proliferation of EC cells.

Objective To investigate the relationship between the changes of C-reactive protein to Albumin Ratio (CAR) levels and Interval Debulking Surgery (IDS) outcome after Neoadjuvant Chemotherapy (NAC) in ovarian cancer patients. Methods A nested case-control study for 209 patients with ovarian cancer who received NAC-IDS therapy from the First Affiliated Hospital of Bengbu Medical College between 2015‒2021 was conducted. Demographic data, laboratory indicators, and imaging examinations were collected. The outcome was regarded as optimal IDS in this study. Univariate and multivariate logistic regression analyses were performed to assess the relationship of CAR before NAC, CAR after NAC and ∆CAR with optimal IDS. The authors also performed the subgroup analysis based on menopausal state. Results The end time of follow-up was January 24, 2022. A total of 156 patients had been treated with optimal IDS, and 53 with suboptimal IDS. After adjusting age, body mass index, menopausal state, NAC drug, peritoneal perfusion and CAR before NAC, the result showed that CAR after NAC (Odds Ratio [OR = 3.48], 95% Confidence Interval [95% CI 1.28‒9.48], p = 0.015) and ∆CAR (OR = 0.29, 95% CI 0.11‒0.78, p = 0.015) were associated with optimal IDS, respectively. Additionally, the authors found a significant correlation between CAR after NAC and optimal IDS (OR = 3.16, 95% CI 1.07‒9.35, p = 0.038), and ∆CAR and optimal IDS (OR = 0.32, 95% CI 0.11‒0.94, p = 0.038) among ovarian cancer patients with menopause. Conclusion CAR after NAC and ∆CAR were independent prognostic markers of optimal interval debulking surgery for ovarian cancer patients.

目的:探讨肿瘤标志物联合超声及恶性肿瘤风险模型(ROMA)指数对卵巢恶性肿瘤的预测作用.方法:回顾性分析197 例因盆腔肿物进行手术病人,依据术后病理报告,112 例为卵巢良性病变(良性组),85 例为卵巢恶性病变(恶性组).对2组病人癌抗原(CA)125、CA153、CA199、甲胎蛋白、癌胚抗原、人附睾蛋白4(HE4)测定结果和超声检查结果、ROMA指数进行统计分析,分析其对卵巢恶性肿瘤的预测作用.结果:超声诊断卵巢良性病变,准确率达 90%以上;卵巢恶性病变组中CA125、CA153、HE4 及ROMA指数较卵巢良性病变组升高,差异均有统计学意义(P＜0.01);CA125、HE4 对卵巢良恶性病变诊断预测价值较高;CA125、HE4、ROMA指数、超声四种指标联合使用对卵巢恶性病变检测的敏感性、特异性、阳性预测值、阴性预测值均较高,大于94%.结论:CA125、HE4、ROMA指数及妇科超声联合对卵巢恶性病变诊断敏感性、特异性、阳性预测值、阴性预测值较高,可作为预测卵巢恶性病变的重要手段.

PURPOSE:To investigate the prognostic value of N6-methyladenosine (m6A)-, 5-methylcytosine (m5C)-, and N1-methyladenosine (m1A)-related genes in cervical cancer (CESC) and predicting immunotherapy response. METHODS:We downloaded cervical cancer mRNA expression profiles, clinical data, and m6A, m5C, m1A-related genes from public databases, and subjected them to serial bioinformatics analysis and clinical sample validation. RESULTS:Differential analysis revealed 106 methylation-related differential genes (MEDs), including 44 differentially downregulated and 62 upregulated genes. We then obtained methylation models containing 10 genes by univariate and multifactorial COX analysis. High risk genes with HR > 1 include IQGAP3, PTBP1, STAC3, CUX1, SLC2A1, and CA2, and low risk genes with HR < 1 include IGBP1, DUOX1, CHAF1A, and STAC3. We verified the accuracy of the model from inside TCGA and outside GSE39001 (AUC = 0.729). K-M analysis showed shorter survival times in the High-risk group, and Immunocytic infiltration analysis showed model genes closely associated with six immune cells. The high-risk group may benefit more effectively from immunosuppressive therapy, especially anti-CTLA-4 therapy (p < .05). We also screened nine drugs for potential treatment and verified the expression of three key genes SLC2A1, CUX1, and CA2 using immunohistochemistry and RT-qPCR experiments with clinical samples. CONCLUSION:We identified a prognostic model using m6A/m5C/m1A-related genes in cervical cancer, which can predict survival time and correlate with immune cell infiltration. Additionally, anti-CTLA-4 may be used as an immunotherapeutic agent for cervical cancer.KEY MESSAGESCervical cancer still has a high mortality rate, we aim to establish a strong prognostic index and new treatment goals for improving patient survival.The role of three types of RNA methylation modifications, m6A, m5C, and m1A, in cervical cancer, remains unknown. Therefore, it is essential to explore the potential molecular mechanisms of m6A, m5C, and m1A methylation regulation in cervical cancer.We also screened nine drugs for potential treatment and anti-CTLA-4 may be used as an immunotherapeutic agent for cervical cancer. We verified the expression of three key genes SLC2A1, CUX1, and CA2.

目的:探讨不同微创手术方式治疗剖宫产术后子宫瘢痕憩室(previous cesarean scar defect,PCSD)的效果.方法:选取144 例PCSD住院病例.根据不同手术方式将其分为阴式手术组(A组,36 例),宫腔镜手术组(B组,71 例),宫腹腔镜联合手术组(C组,37 例).对比3 组病人住院时间、住院费用、术中出血量、术后阴道流血时间、术后病率、术后症状恢复等指标.结果:C组的住院时间和住院费用均高于A组和B组;C组的术后阴道流血时间高于A组和B组,A组的术中出血量高于B组和C组,A组的术后病率高于B组和C组;B组和C组治疗效果显著优于A组,差异均有统计学意义(P<0.05～P<0.01).结论:3 种不同手术方式各有不同的优势且都具有一定的临床效果,阴式手术方式优点是住院时间短、住院费用较低、术后阴道流血时间较短,而缺点是病人术中出血量相对多、术后病率发生高,且术后治愈率相对低;宫腔镜手术方式优点在于住院时间短、住院费用低、术后阴道流血时间短、发生术后病率相对低,且术后治愈率高;宫腹腔镜联合手术方式优点在于术后治愈率最高,但其缺点在于住院费用高,住院时间和术后阴道流血时间长.

Cervical cancer (CC) is the second most common type of cancer in women, and presents a serious threat to public health. We aimed to investigate the regulatory impacts of CDGSH iron-sulfur domain-containing protein 2 (CISD2) in CC and to discuss its relationship with E2F transcription factor 7 (E2F7). With the employment of real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) and western blot, the expression of CISD2 and E2F7 in SiHa cells before or after transfection was estimated. Cell counting kit-8 (CCK-8) assay, Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay, wound healing and transwell were used to detect the proliferation, apoptosis, migration and invasion of SiHa cells. The activity of CISD2 was detected using luciferase report assay and chromatin immunoprecipitation (ChIP) assay was used to confirm the binding of E2F7 and CISD2 promoter. The contents of proliferation- and apoptosis-related proteins were detected using western blot. Results revealed that CISD2 expression was greatly enhanced in CC cell lines. CISD2 depletion inhibited the proliferation, migration and invasion of SiHa cells but promoted the cell apoptosis. It was also found that E2F7 was remarkably elevated in SiHa cells. According to JASPAR database, the binding sites of E2F7 and CISD2 were predicted and ChIP confirmed the binding of E2F7 and CISD2 promoter. Results obtained from luciferase report assay indicated that E2F7 overexpression increased the activity of CISD2 promoter region. Furthermore, further functional experiments demonstrated that the impacts of E2F7 interference on the proliferation, migration, invasion and apoptosis of SiHa cells were reversed by CISD2 overexpression. In summary, CISD2 silence could alleviate the malignant progression of CC and could be transcribed by E2F7.

BACKGROUND:Tumour immune microenvironment (TIME) has long been a key direction of tumour research. Understanding the occurrence, metastasis and other processes of cervical cancer (CC) is of great significance in the diagnosis and prognosis of tumours. METHODS:Here, this study applied the univariate Cox regression model to determine the prognostic association of immune and hypoxia signature genes in CC, and used Least Absolute Shrinkage and Selection Operator (LASSO) Cox method to build immune and hypoxia related risk score model to uncover the immune signature of the TIME of CC. Moreover, we used in vitro experiment to validate the expression level of signature genes. Notably, we assessed the predictive effect of anti-PD1/PDL1 immunotherapy using risk score model. RESULTS:Through the LASSO Cox regression model, we obtained 12 characteristic genes associated with the prognosis of CC, and also associated with immunity and hypoxia. Interestingly, the high-risk group had the properties of high hypoxia and low immunity, while the low-risk group had the properties of low hypoxia and high immunity. In the low-risk group, patients lived longer and had a significant therapeutic advantage of anti-PD-1 immunotherapy. CONCLUSIONS:Established risk scores model can help predict response to anti-PD-1 immunotherapy of CC.

目的:比较智慧教室环境下妇产科学课程教学效果.方法:将同一年级的临床医学专业学生按A、B班随机分配,妇产科学课程的授课采用不同模式,A班为智慧教室环境,为观察组,B班为传统教学环境,为对照组.比较 2 班学生的期末考试成绩,并进行教学效果问卷调查评价其他教学效果指标.结果:A班及格率(≥60 分)和优良率(≥80 分)分别达到100.0%和72.0%,均较B组提高(P<0.05).在整体教学效果、学生妇产科学知识及技能的掌握、学生分析问题及解决问题能力的提升、交流和协作能力的提升、信息化水平及师生互动方面,A班均优于B班,差异有统计学意义(P<0.01).结论:智慧教室环境下妇产科学教学模式总体教学效果优于传统教学模式,学生期末考试及格率及优秀率均得到提升,学生的各方面能力得以提升.

目的 分析病灶切除术治疗局限性子宫腺肌症的疗效及术后复发的高危因素.方法 选取2017年10月至2019年10月蚌埠医学院第一附属医院收治的局限性子宫腺肌症患者120例作为研究对象,对患者进行病灶切除术,观察患者的手术时间、术中出血量、术后排气时间及术后住院时间,手术前后指标变化,复发情况及影响因素.结果 患者手术平均时间(100.70±34.00)min,术中平均出血(172.61±9550)ml,术后平均排气时间(22.14±8.68)h,术后平均住院时间(5.02±1.01)d.与手术前相比,手术后患者子宫体积缩小、痛经评分、月经量评分降低、血红蛋白表达水平升高,血清CA125表达水平降低(P＜0.05),对所有进行病灶切除术的120例患者进行随访,复发率为25.00％(30/120).单因素分析显示,病灶切除术后复发影响因素与痛经评分、术前子宫体积、合并子宫内膜异位症相关,痛经评分较高、术前子宫体积较大、患有合并子宫内膜异位症的患者术后复发率较高,差异具有统计学意义(P＜0.05).Logistic回归分析显示,痛经评分、术前子宫体积、合并子宫内膜异位症为患者术后复发的影响因素(P＜0.05).结论 病灶切除术具有良好的治疗效果,可改善患者月经疼痛程度,并减少月经量.

目的 探讨盆腔三维超声(3D-PFU)检查对女性盆腔器官脱垂(POP)的诊断效能.方法 回顾性分析2021年4月至2022年4月就诊于中国科学技术大学附属第一医院(安徽省立医院)的96例症状性POP女性患者的临床资料,分析盆腔器官脱垂定量(POP-Q)分期法和3D-PFU对POP诊断和分度的结果及相关性.结果 前、中盆腔的POP-Q指示点测量值与3 D-PFU测量值之间呈中等负相关,3 D-PFU可对肛提肌裂孔面积、膀胱移动度、尿道内口闭合状态等参数进行有效测量.结论 3 D-PFU能更全面、直观地评估盆底解剖结构,可作为临床检查的有效补充,指导制定POP个体化的治疗方案.

Lymph node metastasis is an important factor affecting the treatment and prognosis of patients with cervical cancer. However, the comparison of different algorithms and features to predict lymph node metastasis is not well understood. This study aimed to construct a non-invasive model for predicting lymph node metastasis in patients with cervical cancer based on clinical features combined with the radiomic features of magnetic resonance imaging (MRI) images. A total of 180 cervical cancer patients were divided into the training set (n = 126) and testing set (n = 54). In this cross-sectional study, radiomic features of MRI images and clinical features of patients were collected. The least absolute shrinkage and selection operator (LASSO) regression was used to filter the features. Seven machine learning methods, including eXtreme Gradient Boosting (XGBoost), Logistic Regression, Multinomial Naive Bayes (MNB), Support Vector Machine (SVM), Decision Tree, Random Forest, and Gradient Boosting Decision Tree (GBDT) are used to build the models. Receiver operating characteristics (ROC) curve and area under the curve (AUC), accuracy, sensitivity, and specificity were calculated to assess the performance of the models. Of these 180 patients, 49 (27.22

目的:了解高级别宫颈上皮内瘤变(CIN)宫颈锥切术后病灶残留状况及相关影响因素,探讨宫颈锥切术后需要补充手术的必要性.方法:通过回顾性分析因高级别宫颈上皮内瘤变(CINⅡ～Ⅲ)行宫颈锥切术,术后1个月即入院再次行全子宫切除手术的200例病人,通过单因素及logistic回归模型分析探讨年龄、孕产史、HR-HPV基因型、CIN分级、锥切方式、切缘状态与病灶残留之间的相关性.结果:200例病人中,98例(49.0％)子宫切除标本中有残留病变,年龄、产次、锥切方式与术后病灶残留之间无相关性;CIN分级、术前HPV-16或HPV-18阳性、切缘状态与病灶残留呈正相关,logistic回归分析发现CIN分级、切缘阳性和16/18型HPV检测结果均为病变残留的高危因素.结论:CIN分级、切缘阳性和术前HPV-16或HPV-18基因型阳性是高级别CIN行锥切术后病灶残留的可靠预测因子.

目的 探讨育龄期女性HPVE6/E7 mRNA的表达及阴道微生态异常与宫颈病变的关系.方法 选取.2019年12月—2021年8月在蚌埠医学院第一附属医院妇产科门诊及住院部因行宫颈癌筛查就诊的患者,共纳入1 245例育龄患者.所有患者均行阴道镜检查,根据阴道镜病理检查结果分为子宫颈上皮内瘤变(CIN)Ⅰ组290例,CIN Ⅱ组360例,CIN Ⅲ组465例,子宫颈鳞状细胞癌(CECS)组130例,另纳入150例健康女性为对照组.通过检测宫颈HPV E6/E7 mRNA的表达和阴道分泌物病原体,分析其与宫颈疾病的关系.结果 各组阴道清洁度比较差异有统计学意义(x2=46.123,P＜0.05),其中阴道清洁度Ⅲ～Ⅳ检出率对照组＜CIN Ⅰ组＜CIN Ⅱ组＜CINⅢ组＜CECS组;各组加德纳菌阳性检出率差异有统计学意义(x2=39.404,P＜O.05);各组念珠菌阳性及滴虫阳性检出率差异均无统计学意义(均P＞0.05);各组HPV E6/E7 mRNA阳性检出率比较差异有统计学意义(x2=287.386,P＜0.05),CIN Ⅰ 组、CIN Ⅱ 组、CIN Ⅲ 组、CECS 组、对照组 HPV E6/E7 mRNA 阳性检出率分别为 50.7％、81.9％、76.1％、79.2％、13.3％;CIN Ⅰ 组、CIN Ⅱ 组、CIN Ⅲ组、CESC 组中 HPV E6/E7 mRNA 的阳性表达率与阴道微生态失调率相关(均P＜0.05).Logistic回归分析显示阴道清洁度Ⅰ-Ⅱ度及乳酸杆菌阳性是HPV E6/E7 mRNA表达的独立保护因素(OR＜1,均P＜0.05).结论 HPV E6/E7 mRNA的表达阳性率增加、阴道清洁度(Ⅲ～Ⅳ)检出率增加、加德纳菌阳性检出率增加均为预测宫颈癌或宫颈癌前病变的高危因素.

We aimed to study the function and mechanism of endothelial cell-specific molecule 1 (ESM1) in endometrial cancer (EC). The binding relationship between SPI1 and ESM1 was predicted by bioinformatics analysis and verified by the dual-luciferase reporter assay. The expressions and effects of SPI1 and ESM1 were determined using quantitative real-time PCR, immunohistochemistry, Western blot, and functional experiments. ESM1 was highly expressed in EC and was associated with the poor prognosis of patients. ESM1 silencing suppressed the viability, proliferation, invasion, and angiogenesis of EC cells, down-regulated expressions of PCNA, N-cadherin, Vimentin, VEGFR-1, VEGFR2, and EGFR, but upregulated E-cadherin level, while ESM1 overexpression did oppositely. Moreover, SPI1 bound to ESM1. Overexpressed SPI1 promoted the expression of ESM1 and induced malignant phenotype (viability, proliferation, and invasion), which were countervailed by ESM1 silencing. Collectively, ESM1 induced by SPI1 promotes the malignant phenotype of EC.

The primary regulatory gene for fatty acid synthesis, stearoyl-CoA desaturase 1 (SCD1), has been linked to the progression of several malignancies. Its role in cervical cancer remains unclear till now. This paper aimed to explore the role and mechanism of SCD1 in cervical cancer. The GEPIA database was used to perform a bioinformatics analysis of the role of SCD1 in cervical cancer staging and prognosis. The influences of SCD1 knockdown on cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) progress were then investigated. Following transcription factor Kruppel like factor 9 (KLF9) was discovered to be negatively correlated with SCD1, the regulatory role of KLF9 in the effects of SCD1 on cervical cancer cells and the signaling pathway was evaluated. According to the GEPIA database, SCD1 level was associated with the cervical cancer stage, the overall survival level, and the disease-free survival level. Cell proliferation, migration, invasion, and EMT progress were all hindered when its expression was knocked down. Novelty, KLF9 reversed the effects of SCD1 on cells, as well as the Akt/glycogen synthase kinase 3β (GSK3β) signaling pathway. Together, SCD1 was negatively regulated by KLF9 and it activated the Akt/GSK3β signaling pathway to promote the malignant progression of cervical cancer cells. Developing SCD1 inhibitors offers novel ideas for the biological treatment of cervical cancer.

Abstract Prostaglandin E2 (PGE2) is a potent lipid mediator of inflammation that modulates immune cell function by binding to unique G protein‐coupled receptors (EP receptors). PGE2 production increases during microbial infection and inflammation. In this study, we assessed the effect of PGE2 on the phagocytosis of bacteria by neutrophils, which are key players during infection and inflammation. We also looked for specific EP receptor signaling pathways that contributed to the neutrophil phagocytic activity. PGE2 (50–1000 ng/ml) inhibited the phagocytosis of Escherichia coli by HL‐60 human neutrophils in a concentration‐dependent manner. Inhibition of neutrophil phagocytosis by PGE2 correlated with increased intracellular cyclic adenosine monophosphate (cAMP) production, and forskolin, an adenosyl cyclase agonist, confirmed the inhibitory effect of cAMP stimulation on neutrophil phagocytosis. The expression of EP2 receptors by HL‐60 cells was confirmed by western blot analysis, and selective agonism of EP2 receptors mimicked the inhibition of phagocytosis by PGE2. The EP2 receptor antagonist AH‐6089 partially blocked the inhibition of neutrophil phagocytosis PGE2. Specific inhibition of phosphatase and tensin homolog (PTEN) enzyme attenuated the inhibition of neutrophil phagocytosis by PGE2, and both PGE2 and increased intracellular cAMP increased neutrophil PTEN activity, which was associated with decreased PTEN phosphorylation. The results support negative regulation of the antimicrobial activity of neutrophils (i.e., phagocytosis), which has important implications for the future management of bacterial infections.

Objective:To study the clinical effect of sacrospinous ligament fixation (SSLF) and traditional vaginal hysterectomy on pelvic organ prolapse (POP) .Methods:A retrospective analysis was performed on 68 patients with POP of degree II-IV admittedl from Jan. 2017 to Dec. 2019. Among them, 33 patients were treated with SSLF (observation group) and 35 patients were treated with vaginal total hysterectomy (control group). Intraoperative blood loss, operative time, postoperative indwelling catheter and average length of hospital stay were compared between the two groups. The patients were followed up for 6 months, and the scores of pelvic floor dysfunction questionnaire-20 (PFDI-20) and sexual quality questionnaire -12 (PISQ-12) were used to evaluate the subjective satisfaction degree of postoperative recovery.Results:In the observation group, the intraoperative blood loss (173.94±52.14) ml, postoperative indurating catheter time (2.72±0.45) d and average length of hospital stay (7.09±0.63) d were observed. There were statistically significant differences in intraoperative blood loss (228.86±53.40) ml, postoperative induration time (4.54±0.61) d and mean hospital stay (9.22±0.81) d in the control group ( P<0.05). There was no significant difference in the operation time between the observation group (99.57±9.50) min and the control group (101.06±8.64) min, ( P>0.05). The improvement of PFDI-20 and PISQ-12 in both groups was significant before and after treatment. The PISQ-12 score of the observation group was higher than that of the control group 6 months after surgery, and the difference was statistically significant ( P <0. 05). There was no significant difference in PFDI-20 score between the two groups ( P>0.05). There was statistical significance in the positions of pop-Q indicators in the two surgical methods ( P < 0.05) . Conclusions:SSLF with uterus preservation and total vaginal hysterectomy are both effective in treatment of moderate and severe POP. However, SSLF with uterus preservation has less intraoperative blood loss, and the postoperative recovery is significantly better than that with total vaginal hysterectomy. In addition, it satisfies patients’ desire to preserve uterus, improves the postoperative sexual life quality, which is worthy of promotion.

目的 探讨人乳头瘤病毒(HPV)E6/E7 mRNA检查在宫颈细胞学检查结果为意义不明确的非典型鳞状上皮细胞(ASCUS)患者中的分流价值.方法 收集2019年8月-2020年8月就诊于蚌埠医学院第一附属医院妇科,液基薄层细胞学(TCT)为ASCUS的患者,行(HPV)DNA检查、(HPV)E6/E7 mRNA检查,阳性者行阴道镜检查,对检测结果及宫颈活检结果进行统计学分析,探讨HPV E6/E7 mRNA检查在TCT结果为ASCUS患者中的分流价值.结果 231例ASCUS患者分为宫颈慢性炎117例(50.65％),宫颈低级别上皮内病变65例(28.14％),宫颈高级别上皮内病变47例(20.35％),宫颈癌2例(0.87％).HPV E6/E7 mRNA阳性91例(39.39％),HPV DNA阳性127例(54.98％),二者对C1N11+的检出率相比,差异无统计学意义.(HPV)E6/E7 mRNA检查CIN II+(即HISL+SCC)特异度为69.2％,灵敏度为71.4％,阴性预测值为90.0％,阳性预测值为38.5％;HPV DNA检查CIN II+(即HISL+SCC)特异度是53.3％,灵敏度为85.7％,阴性预测值为93.3％,阳性预测值为33.1％.(HPV)E6/E7 mRNA检查的特异度明显高于(HPV)DNA,差异有统计学意义(X2 = 26.273,P＜0.001).灵敏度相比,差异无统计学意义.将不同宫颈病变患者的copy值进行统计与分析,随着病变级别的进展,(HPV)E6/E7 mRNA的copy值也随之增加,差异有统计学意义(p =0.562,P ＜0.001).(HPV)E6/E7 mRNA检查的AUC为0.69,大于(HPV)DNA的AUC值(0.60),与(HPV)DNA检查相比诊断效率更高.结论 (HPV)E6/E7 mRNA检查对于TCT结果为意义不明确的非典型鳞状上皮细胞(ASCUS)患者是一种良好的分流手段,能降低阴道镜转诊率,减轻患者负担及临床医师工作量,避免漏诊和过度治疗,可成为ASCUS患者分流的新方法.

目的:分析妇科腹腔镜手术中转开腹发生情况及其影响因素.方法:采用回顾性分析方法,收集2014-2018年蚌埠医学院第一附属医院2683例妇科腹腔镜手术病人资料,根据已实施腹腔镜手术方式,分为4组:Ⅰ类(腹腔镜检查或附件手术),Ⅱ类(卵巢囊肿剥除术或卵巢及附件切除术),Ⅱ类(子宫肌瘤剔除术、子宫次全或全切除术),Ⅳ类(广泛子宫切除术+盆腔淋巴结清扫术).分析中转开腹病人的手术方式及中转开腹的原因.结果:2683例腹腔镜手术病人,中转开腹22例,中转开腹率0.81％.其中Ⅰ～Ⅳ类手术中转开腹率分别为0.36％(6/1680)、0.97％(3/310)、1.52％(10/660)9.09％(3/33).4组不同手术方式中转开腹率不同,Ⅰ类、Ⅱ类和Ⅱ类均低于Ⅳ类,差异有统计学意义(P<0.01).中转开腹原因依次为重度粘连36.36％(8/22),特殊部位病变22.73％(5/22),出血18.18％(4/22),术中诊断的恶性肿瘤13.64％(3/22),误伤9.09％(2/22).结论:妇科腹腔镜手术中转开腹的发生主要与手术操作的难易程度及需要手术的范围密切相关.严格术前评估和手术适应证的遴选、采用适宜的手术方式、提高术者操作技能是预防妇科腹腔镜中转开腹发生的有效方法.

OBJECTIVE:To investigate the role of fucoxanthin, reported to have significant anticancer effects, and histone Cluster 1 H3 Family Member D (HIST1H3D; implicated in tumorigenesis) in cervical cancer.METHODS:The half maximal inhibitory concentration (IC50) of fucoxanthin against HeLa and SiHa cervical cancer cells was determined. Differentially expressed genes (DEGs) in SiHa cells treated with IC50 fucoxanthin were screened by high-throughput techniques and subjected to signal enrichment. Following identification of HIST1H3D as a candidate gene, HIST1H3D-knockdown models were created via transfection with a short hairpin HIST1H3D payload. Impacts on cell proliferation, cell-cycle distribution, colony formation, and apoptosis were studied.RESULTS:The fucoxanthin IC50 was 1 445 and 1 641 µM (Hela and SiHa cells, respectively). Chip results revealed 2 255 DEGs, including 943 upregulated and 1 312 downregulated genes, in fucoxanthin-treated versus untreated SiHa cells. Disease and function analysis indicated that these DEGs are primarily associated with cancer and organismal injuries and abnormalities, and online integrated pathway analysis showed that the DEGs were mainly enriched in p53 signalling. HIST1H3D was significantly downregulated in response to fucoxanthin. Inhibition of HIST1H3D mRNA significantly reduced cell proliferation and colony formation, significantly augmented the percentage of apoptotic HeLa and SiHa cells, and cells were arrested in G0/G1 cell cycle phase.CONCLUSION:The results suggest that HIST1H3D may be an oncogene in cervical carcinogenesis and a potential fucoxanthin target in treating cervical cancer.