目的 利用癌症基因组图谱(TCGA)数据库中的数据分析SEC61G在浸润性乳腺癌中的表达情况及其与临床预后的关系.方法 从TCGA数据库中收集1 222个浸润性乳腺癌患者的RNAseq数据,用Wilcoxon秩和检验分析SEC61G在正常组织和肿瘤组织中表达的差异,采用logistic回归分析SEC61G的表达与临床病理特征的关系,使用Kaplan-Meier方法和Cox回归分析评估SEC61G在预后中的作用.结果 浸润性乳腺癌组织中SEC61G的表达明显高于正常乳腺组织(P＜0.001).T分期(P=0.028)、N分期(P=0.009)、病理分期(P=0.003)、ER 状态(P＜0.001)、PR 状态(P＜0.001)、HER2 状态(P=0.025)、组织学类型(P＜0.001)与 SEC61G 表达显著相关.单因素Cox回归分析显示SEC61G高表达与DSS有相关性(P＜0.001,HR=0.426,95％CI=0.272～0.668).多因素Cox分析显示SEC61G高表达是疾病特异生存率(DSS)的独立危险因素(P=0.009,HR=0.479,95％CI=0.276～0.831).SEC61G的表达与浸润性乳腺癌的免疫浸润程度有关(P＜0.05).各组的K-M曲线显示肿瘤组织中SEC61G表达越高浸润性乳腺癌患者的预后越差(P＜0.05).结论 SEC61G高表达与浸润性乳腺癌预后不良有关,可以作为预测浸润性乳腺癌患者生存有效的生物标志物.

目的 探讨人乳头瘤病毒(HPV)E6/E7 mRNA检查在宫颈细胞学检查结果为意义不明确的非典型鳞状上皮细胞(ASCUS)患者中的分流价值.方法 收集2019年8月-2020年8月就诊于蚌埠医学院第一附属医院妇科,液基薄层细胞学(TCT)为ASCUS的患者,行(HPV)DNA检查、(HPV)E6/E7 mRNA检查,阳性者行阴道镜检查,对检测结果及宫颈活检结果进行统计学分析,探讨HPV E6/E7 mRNA检查在TCT结果为ASCUS患者中的分流价值.结果 231例ASCUS患者分为宫颈慢性炎117例(50.65％),宫颈低级别上皮内病变65例(28.14％),宫颈高级别上皮内病变47例(20.35％),宫颈癌2例(0.87％).HPV E6/E7 mRNA阳性91例(39.39％),HPV DNA阳性127例(54.98％),二者对C1N11+的检出率相比,差异无统计学意义.(HPV)E6/E7 mRNA检查CIN II+(即HISL+SCC)特异度为69.2％,灵敏度为71.4％,阴性预测值为90.0％,阳性预测值为38.5％;HPV DNA检查CIN II+(即HISL+SCC)特异度是53.3％,灵敏度为85.7％,阴性预测值为93.3％,阳性预测值为33.1％.(HPV)E6/E7 mRNA检查的特异度明显高于(HPV)DNA,差异有统计学意义(X2 = 26.273,P＜0.001).灵敏度相比,差异无统计学意义.将不同宫颈病变患者的copy值进行统计与分析,随着病变级别的进展,(HPV)E6/E7 mRNA的copy值也随之增加,差异有统计学意义(p =0.562,P ＜0.001).(HPV)E6/E7 mRNA检查的AUC为0.69,大于(HPV)DNA的AUC值(0.60),与(HPV)DNA检查相比诊断效率更高.结论 (HPV)E6/E7 mRNA检查对于TCT结果为意义不明确的非典型鳞状上皮细胞(ASCUS)患者是一种良好的分流手段,能降低阴道镜转诊率,减轻患者负担及临床医师工作量,避免漏诊和过度治疗,可成为ASCUS患者分流的新方法.

目的:探讨TAC化疗联合DC和CIK生物免疫治疗三阴性乳腺癌的临床疗效.方法:将收治的136例三阴性乳腺癌患者,随机分为观察组(67例)和对照组(69例),观察组将三阴性乳腺癌根治性手术后的患者采用TAC 6个疗程化疗联合DC和CIK 3个疗程生物免疫治疗,对照组采用TAC 6个疗程单纯化疗.观察比较2组患者的1年、3年无病生存率(DFS)及3年总的生存率(OS).结果:观察组3年总生存率70.1％,1年无病生存率83.6％,3年无病生存率61.2％.对照组3年总生存率47.8％,1年无病生存率66.7％,3年无病生存率33.3％;观察3年总生存率及1年无病生存率高于对照组,但差异无统计学意义(P>0.05),3年无病生存率观察组优于对照组,2组比较差异有统计学意义(P<0.05).结论:TAC化疗联合DC和CIK生物免疫治疗三阴性乳腺癌,明显优于单纯的TAC方案化疗,可增加患者无病生存的机会,改善患者的生存质量.

Objective To investigate care on patients with esophageal carcinoma with intensity modulated radiation therapy( IMRT),to prevent and alleviate the side effects of radiotherapy on patients with esophageal carcinoma,to ensure the smooth completion of the radiotherapy treatment planning of patients and to improve quality of life of patients. Methods To careful nursing,from mental nursing irradiated skin care,diet nursing care,nursing of radiation esophagitis,prevention of bone marrow suppression and nursing care of radioactive were conducted. Results The results of all 45 patients successfully completed the entire process of radiotherapy,had recently reached 95. 6 percent efficiency. While all the patients with lower radioactive esophagitis and pneumonitis and cutaneous radiation reaction of light,also with lower incidence of myelosuppression. Conclusion Careful clinical nursing was one of the key factors to alleviate the side effects of radiotherapy and ensure the smooth completion of the radiotherapy treatment planning of patients.

?Objective:To study the relation between serum ceruloplasmin oxidase(sCP) activity and tumors in gynecology.Methods:The sCP activity was detected in 30 patients with benign tumor,47 patients with malignant tumor,12 with malignant tumor after operation and 125 healthy women.Results:The sCP activity in patients with malignant tumor was obviously higher than that in patients with benign tumors,the patients with malignant tumors after operation and healthy individuals( P 0.01).However the sCP activity showed no significant difference among the patients with benign tumors,the patients with malignant tumors after operation and the healthy individuals( P 0.05).Conclusions:The detection of sCP activity is helpful in the differentiating benign and malignant tumors and can be used as a parameter in watching the changes in malignant tumors.