OBJECTIVE:Discussed the protection of taxane-derived compounds, 7-deacetyl-taxine B and 5-cinnamoyloxy-taxin B, against oxidative stress injury. METHODS:SK-N-SH cells were pretreated with 7-deacetyl-taxine B, 5-cinnamoyloxy-taxin B or DMSO (control) and then incubated with H2O2 for another 24 h. Cell viability was measured by MTT colorimetric assay. Apoptosis rate, reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were detected using flow cytometry assay. Ultrastructure was detected by Transmission electron microscope. RESULTS:Taxane-derived compounds improved H2O2 mediated loss of cell viability in SK-N-SH cell. Ultrastructure of control group cells showed nucleus shrinkage, dense aggregation of chromatin, appearance of apoptotic body and mitochondrial dilation. Treated group showed alleviation of mitochondrial dilation and chromatin margination, and reduction of apoptosis cells induced by H2O2. The apoptosis rate was decreased in treated group compared with control group. DCFH-DA staining showed that ROS were significantly decreased in cells treated with taxane-derived compounds compared with control group. Rhodamine123 staining showed that MMP was significantly decreased in cells treated with taxane-derived compounds compared with control group. CONCLUSION:The anti-oxidative stress and neuroprotective effect of taxane-derived compounds was verified here, wherein it could protect neurocytes from H2O2-induced oxidative stress injury.

Objective To investigate the correlation between patent posterior communicating artery(PCoA) and proximal vertebral artery stenosis in patients with atherosclerotic carotid artery stenosis disease(≥70%).Methods Examine the intracranial artery and carotid artery by using color Doppler flow imaging(CDFI) in 160 patients with carotid arterial stenosis(≥70%) confirmed by angiography before and after surgical treatment(endarterectomy,carotid artery stent placement).The patients were divided into patients with PCoA flow group and patients without PCoA flow group,with occurrence rate of proximal vertebral artery stenosis compared between the two groups.Results(1) Flow velocity at proximal vertebral artery before operation(with PCoA flow) in patients with PCoA flow group was significantly improved compared to that after treatment(without PCoA flow).Peak systolic velocity(PSV) and end diastolic velocity(EDV) values of proximal vertebral artery with and without PCoA flow were 100.76±74.84cm/s,81.54±54.29cm/s(P=0.000).(2) Patients with PCoA flow accounted for 50%(80/160),including 45.6%(73/160) with stenosis of proximal vertebral artery;Patients without PCoA flow accounted for 50%(80/160),including 28.1%(45/160) with stenosis of proximal vertebral artery(P=0.002).Conclusion Proximal vertebral artery stenosis rate is significantly higher in patients with severe carotid artery stenosis disease with patent PCoA.