Herein, a composite hydrogel based on two natural macromolecules-chitosan (CS) and γ-poly(glutamic acid) (γ-PGA)-was developed, crosslinked by zeolitic imidazolate framework-8 (ZIF-8) nanoparticles, which serve as reactive crosslinking hubs to covalently connect the biopolymer chains for efficient immobilization of α-glucosidase (α-Glu). A carboxyl-terminated poly(ethylene glycol)-poly(glutamic acid) copolymer was first synthesized and its activated carboxyl groups underwent amidation with amino groups of CS to rapidly form a three-dimensional PC/ZIF-8 hydrogel. The effects of ZIF-8 content and CS concentration on micro-morphology, swelling behavior, and rheological properties were systematically investigated. ZIF-8-mediated crosslinking was essential for gel formation, enabling effective modulation of network density and pore architecture. The optimized hydrogel showed no inhibitory effect on α-Glu. Immobilized α-Glu@PC/ZIF-8 exhibited enhanced pH stability (retaining 53% and 70% activity at pH 4.0 and 9.0), outstanding storage stability (>90% after 4 weeks), excellent organic solvent tolerance (>90% in most solvents), and good reusability (near 100% activity over 6 cycles). Mass transfer studies confirmed that the designed crosslinking density provided efficient substrate/product diffusion channels, minimizing product inhibition. Hansen solubility parameter analysis revealed a synergistic "thermodynamic exclusion + size-sieving" mechanism for solvent tolerance. Coupled with HPLC, the immobilized enzyme successfully identified two α-glucosidase inhibitors-batatasin I and 2,4-dimethoxy-6,7-dihydroxyphenanthrene-from Dioscorea opposita Thunb. peel. This work demonstrates that rational chemical modification of natural macromolecular networks via MOF crosslinkers provides an effective strategy for constructing high-performance enzyme immobilization platforms.

A novel hydrophobic deep eutectic solvent (HDES) composed of menthol and lactic acid was successfully synthesized and applied for the simultaneous vortex-assisted liquid-liquid microextraction (VA-LLME) of triterpenoids (glycyrrhizic acid, GL, and glycyrrhetinic acid, GA) and a flavonoid (chalcone A, ChA) from licorice extract. Under the optimized conditions using 5 mL of plant extract and 800 µL of HDES, the target analytes were efficiently extracted via vortex mixing and centrifugation, with the resulting HDES phase directly analyzed by high-performance capillary electrophoresis (HPCE). The proposed method demonstrated excellent linearity (3-600 µg mL-1, R2 > 0.9954) for all analytes. The limits of detection (LODs) and quantification (LOQs) ranged from 0.67 to 2.97 µg mL-1 and 2.22 to 9.90 µg mL-1, respectively. Intra-day and inter-day precision (RSD) were below 4.21% and 6.41%, with recoveries between 91.51% and 105.1%. The method is relatively sensitive, showing particularly good enrichment for GL and GA in licorice, and can be applied to the purification of triterpenoids from this herb. Overall, this approach is simple, rapid, environmentally friendly, and has been successfully applied to the accurate quantification of triterpenoids and flavonoids in licorice extract.

Batatasin (a natural antioxidant ingredient) is expected to have an effect on the release of reactive oxygen species from the mitochondria of living cells. Herein, we designed a novel WO3@Bi-BiVO4 Z-scheme photoelectrode to sensitively detect this effect using HeLa cells as models for common oxidative stress-related diseases.

Aicao (Artemisia argyi) and honey are herbal medicines for the regulation of digestion and various major diseases driven by compounds, but the effect of processing-generated particles remains elusive. Our aim is to investigate the Aicao/Honey (AC/H) complex-particle formation and to test whether different hydrothermal synthesis temperatures produce particles with distinct physicochemical and biological properties that contribute to cytotoxicity via known or novel pathways. For methods, we processed AC/H complexes at 150℃ and 200℃ and investigated characteristics as potential honey-infused or carbon-dot-like synthesis with imaging potential. The nanoparticles are approximately 100 nm or smaller in size, possess negatively charged surfaces and red fluorescence, and can enter the Golgi apparatus within cells. The two synthesized AC/Hs exhibit a distinct degree of regulation of peroxidase activities, cytotoxicity, and nanozyme (herbzyme) of phosphatase enhancement, which is linked to promoting AKT kinase inhibitor efficiency with targeting AKT phosphorylation at Ser473 but not Thr308 or decreased AKT levels. Consistently, the compound-target enrichment analysis suggests the AC/H in targeting the PI3K/AKT pathway and potent compound docking. Importantly, the AC/H-induced cytotoxicity was disrupted by the ferroptosis inhibitor Fer-1 and abolished upon ROS inhibition by NAC, while the AC/H stimulated ROS production and regulated ferroptosis-associated GPX4. Thus, the different temperature-processed AC/H requires ROS and ferroptosis for inducing cytotoxicity, with phosphatase-like enhancing activity for targeting phosphorylation in cancer cell death. Our studies highlight the particle effect of AC/H by different hydrothermal processing with potential applications in pharmaceuticals or biomedicine as a non-food industrial use.

Background Efficient immobilization of α-glucosidase (α-Glu) is crucial for its application in inhibitor screening, but conventional polysaccharide hydrogels suffer from limited mechanical strength and stability. We hypothesized that a hybrid hydrogel synergistically integrating covalent polymer cross-linking with metal-organic framework (MOF) nanoparticles would overcome these limitations. Results ZIF-8 reinforced alginate-chitosan (SC/ZIF-8) microspheres were fabricated via NHS/EDC-mediated covalent cross-linking and Ca2+-induced gelation. ZIF-8 incorporation dramatically enhanced mechanical strength (complex modulus increased >5-fold) and modulated swelling. The immobilized α-Glu@SC/ZIF-8 preserved intrinsic enzyme activity, exhibited superior pH stability (retaining ∼90% activity at pH 9.0), and demonstrated exceptional reusability (nearly 100% activity after 13 cycles) and storage stability (>95% after 28 days). Coupled with HPLC-MS/MS, the platform successfully identified batatasin I and 2,4-dimethoxy-6,7-dihydroxyphenanthrene as α-glucosidase inhibitors from Dioscorea opposita Thunb. peel extracts. Significance This work presents a robust, biocompatible, and reusable biocatalytic platform that addresses key limitations of existing immobilization systems. The SC/ZIF-8 hybrid strategy offers a versatile foundation for enzyme-based screening in natural product drug discovery.

We developed a photoelectrochemically driven sensor to indirectly monitor superoxide anions (O-2(center dot-)) through direct detection of H2O2, which is readily converted from O-2(center dot-) immediately after its production. NiCo hydroxide nanowires were hydrothermally grown on a Ti substrate and then transformed into evenly-distributed ultra-thin NiCo-metal organic framework (MOF) nanosheets (NSs) with a diameter of similar to 1 mu M. Then, high-density Al doped MnO2 flakes (20-30 nm) were also hydrothermally synthesized on both sides of NiCo-MOF NSs to generate a core-shell heterojunction with a large specific surface area. Ascribed to the verified well-matched energy levels and tight physical contact between the two materials, the heterojunction effectively promoted the separation of photo-generated charges. Based on the photoelectrochemical energy device with Al-MnO2@NiCo-MOF heterojunction as the photoelectrode, the sensor exhibited a much higher photocurrent (similar to 11.5 mu A) than Al-MnO2 (similar to 3.8 mu A) and NiCo-MOF/Ti (similar to 2.0 mu A) at identical test conditions, a dynamic range of 0.1-50000 nM and low detection limit of 0.038 nM for H2O2. Furthermore, the sensor has enabled a convenient, sensitive and rapid measurement of O(2)(center dot-)leaked from the mitochondrial complexes of Hela cells. The simple comparison of signals determines that oxidized ubiquinone in complex III is the main electron leakage site in Hela mitochondria.

Abnormal concentrations of hydrogen peroxide (H 2 O 2 ) are toxic to living cells and may induce a number of diseases. Herein, a self‐powered miniaturized biosensor (SPB) based on an enzyme biofuel cell is constructed to monitor H 2 O 2 . This SPB significantly minimized the use of bioenzymes that often experience instability and lead to the high cost of biosensors. More specifically, a composite of polydopamine (PDA)‐gold nanoparticles (AuNPs) is prepared as an anodic catalyst scaffold to immobilize glucose oxidase to efficiently catalyze the oxidation of glucose (fuel) due to its excellent biocompatibility and electrical conductivity. Upon the incorporation of CuCoP with a polyoxometalate H 3 PW 12 O 40 (PW 12 ), a nanoenzyme of CuCoP‐PW 12 composite is realized as a non‐biological cathodic catalyst to replace the conventional cathode enzymes for the reduction of H 2 O 2 . The abundant catalytic active sites on CuCoP‐PW 12 and high electron transfer rate of PW 12 result in a high catalytic activity toward H 2 O 2 reduction at the cathode. Owing to a good synergy between the bioanode and abiotic‐cathode, the prepared SPB exhibits two linear ranges (2–20 and 20–50 µ m ) and a low detection limit (0.0589 µ m ) toward H 2 O 2 detection. Upon the use of H 2 O 2 as a model analyte, this work demonstrates that SPB can be effectively applied in biomedical sensing.

Due to the larger pore structure, the macroporous material can be used as the immobilized carrier to not only increase the enzyme loading capacity, but also facilitate the transfer of reactants and substrates. Based on this, a three-dimensional ordered macro-microporous ZIF-8 (SOM-ZIF-8) was prepared using three-dimensional ordered stacked polystyrene spheres as the hard template. The morphology and structure of SOM-ZIF-8 were characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray diffraction (XRD), fourier transform infrared spectroscopy (FT-IR) and so on. The macropore size of SOM-ZIF-8 was tested to be about 100 nm by N2 adsorption-desorption isotherms. Then alpha-glucosidase (alpha-Glu) was encapsulated into the macropore of SOM-ZIF-8 by physical adsorption method to prepare the immobilized enzyme microreactor. Under the optimal immobilization conditions, the loading capacity of SOM-ZIF-8 to alpha-Glu reached 113.42 mu g/mg. Due to the encapsulation in the three-dimensional macropores, the conformational changes of the enzyme are restricted, endowing the immobilized enzyme with excellent acid and alkali resistance, a long storage time, and almost unchanged relative activity after 7 cycles. Finally, the SOM-ZIF-8-alpha-Glu microreactors combined with high performance liquid chromatography (HPLC) were applied to offline screen alpha-Glu inhibitory active components from tea extract. Several components including gallocatechin, catechin and epicatechin gallate were successfully screened out, which verified the application feasibility of the immobilized enzyme microreactor.

Flavonoids are bioactive components in natural products, which possess anti-inflammatory, antibacterial, antioxidant, and cardiovascular protective properties. However, due to the complexity and low content of the components in these samples, developing rapid and sensitive methods for the isolation and extraction of flavonoids still remains a challenge in medical and food science. Herein, a 4-formylphenylboronic acid functionalized magnetic Fe3O4 nanomaterial (Fe3O4@FPBA) was synthesized and applied as a sorbent of magnetic solid-phase extraction (MSPE) to covalently extract flavonoids from leaves of Lonicera japonica Thunb.. The structure, morphology and magnetic properties of Fe3O4@FPBA particles were characterized by fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), vibrating sample magnetometer (VSM) and scanning electron microscope (SEM) technologies. Employing luteoloside, luteolin, lonicerin, hyperoside, quercetin and rutin as model compounds, Fe3O4@FPBA as sorbent, a MSPE coupling with capillary electrophoresis (CE) method was developed and optimized to detect the flavonoids. Adsorption kinetics display that the adsorption of flavonoids by Fe3O4@FPBA is in line with the Quasi-second-order model, which is controlled by chemisorption mechanism, with the equilibrium adsorption capacity ranging from 3.66 to 6.16 mg/g. The isothermal adsorption model shows that the adsorption is more consistent with Freundlich isotherm equation, and the exponent n is around 1. In addition, the material was applied to the leaves of Lonicera japonica Thunb. extract. Four kinds of flavonoids and three other o-hydroxyl compounds were covalently extracted and magnetically separated. Moreover, the material can still maintain high adsorption properties after recycling 5 times. The material possesses strong magnetism and boric acid ligands, which can realize rapid and high-capacity separation and enrichment of flavonoids in liquid samples. Therefore, the strategy offers an innovative method for the extraction and purification of flavonoids from complex natural plants and also provides a research basis for the discovery of new medicinal compounds based on natural products.

Cattail grass biomass is used as the precursor for preparing both bi-functional cathode catalyst and solid electrolyte in the construction of high-performance Zn-air battery (ZAB). First, cattail grass is transformed into porous carbon micron tubes (CMTs), which are immobilized by polyoxometalate clusters of (H2bimb)3 {Cd(P4Mo6O31H8)2}·5.5H2O (bimb = 4,4-di(1H-imidazole-1-yl)-1,1-biphenyl) (abbreviation: {Cd(P4Mo6)2}), and then calcinated in N2 to form Mo2C-MoO2/CMTs. {Cd(P4Mo6)2} not only acts as the Mo source for the formation of Mo2C-MoO2 heterojunction but also provides the Mo species to in situ etch carbon tubes to generate the porous microstructures. In ZABs, the multi-catalytic sites (Mo2C and MoO2) can both facilitate the oxygen reduction / evolution reaction (ORR/OER) catalytic performance at the battery cathode, and inhibit the formation of zinc dendrites by forming a large number of nucleation sites on the battery anode. Density Functional Theory results have verified the synergistic effect between Mo2C and MoO2, indicating that multiple active sites with superior catalytic effect than the individual materials. The ZABs assembled with Mo2C-MoO2/CMTs exhibit a peak power density of 264.09 mW cm-2. Cattail grass is also used to prepare highly conductive gel electrolyte to assemble all-solid coin cell ZABs with high capacity of 97.33 mW cm-2 and 100 h of charge-discharge stability.

Excessive reactive oxygen species (superoxide anion and H2O2) induce oxidative stress in living cells, leading to the destruction of intracellular biological macromolecules and a series of diseases. Therefore, a sensitive, convenient and fast detection technique is required to monitor their levels. In this work, we designed and assembled a nanozyme-bioenzyme composite based biosensor by in-situ growth of horseradish peroxidase (HRP) embedded metal-organic framework (ZIF-67) on NiCo2O4 nanowire arrays (NW) modified carbon cloth of using a one-step strategy. A sugar gourd-like core-shell morphology of ZIF-67(HRP)|NiCo2O4NW|CC was obtained by adjusting the ratio of Co2+ with 2-methylimidazole to obtain a balance between the protection of HRP and ion/ charge transport. Benefiting from the synergistic effect between the embedded HRP and exposed Co/Ni active sites, the sensor exhibited excellent catalytic performance for H2O2 with a detection range of 0.03-1000 mu M, a detection limit of 0.038 mu M, a sensitivity of 170 mu A center dot mM- 1 center dot cm-2, good selectivity and long-term stability. Due to the fast conversion of unstable O2 center dot- to H2O2, the as-prepared H2O2 sensor was also used to indirectly monitor the O2 center dot- and thus determine the electron leakage sites in mitochondria of liver cancer cells. To increase the detection reliability, the complexes of isolated mitochondria from live HepG2 cells were respectively inhibited by four mitochondrial inhibitors to further boost the leakage of electrons. The chronoamperometry results showed that the electrons may leak out from complex I/III in the mitochondria. This study could be beneficial to diagnosis of oxidative stress diseases and provide meaningful information for their treatment.

Zn-doped carbon dots (Zn@C-210 calcination temperature at 210 °C and Zn@C-260 calcination temperature at 260 °C) were synthesized via an in situ calcination method using zinc citrate complexes as precursors, aiming to investigate the mechanisms of their distinctive fluorescence properties. A range of analytical methods were employed to characterize these nanomaterials. The mechanism study revealed that the coordination structure of Zn-O, formed through zinc doping, can induce a metal–ligand charge-transfer effect, which significantly increases the probability of radiative transitions between the excited and ground states, thereby enhancing the fluorescence intensity. The Zn@C-210 in a solid state and Zn@C-260 in water exhibited approximately 71.50% and 21.1% quantum yields, respectively. Both Zn@C-210 and Zn@C-260 exhibited excitation-independent luminescence, featuring a long fluorescence lifetime of 6.5 μs for Zn@C-210 and 6.2 μs for Zn@C-260. Impressively, zinc-doped CDs displayed exceptional biosafety, showing no acute toxicity even at 1000 mg/kg doses. Zn@C-210 has excellent fluorescence in a solid state, showing promise in anti-photobleaching applications; meanwhile, the dual functionality of Zn@C-260 makes it useful as a folate sensor and cellular imaging probe. These findings not only advance the fundamental understanding of metal-doped carbon dot photophysics but also provide practical guidelines for developing targeted biomedical nanomaterials through rational surface engineering and doping strategies.

As common complications of diabetes, long-term hyperglycemia and inflammatory infiltration often lead to prolonged unhealing of chronic diabetic wounds. The natural hydrogel-containing plant polysaccharides were recorded to have effective hypoglycemic and anti-inflammatory effects. This study focused on the accelerating effect of diabetic wound healing of hydrogels doped with Dioscorea opposita polysaccharide (DOP)-calcium carbonate (CaCO3) microspheres, which have glucose-responsive insulin release and anti-inflammatory effects. The hydrogel defined as PL-PVA/DOP-CaCO3 was designed via the borate ester bonds between polylysine-phenylboronic acids (PL-PBA) and dihydroxyl groups of poly(vinyl alcohol) (PVA). DOP modified on the surface of CaCO3 microspheres can simultaneously act with PBA to dope into the PL-PVA hydrogel and maintain glucose sensitivity. The mechanical and swelling properties of the hybrid hydrogels were reinforced by the incorporated microspheres. Meanwhile, the hyperglycemia was also regulated by the released insulin and DOP. The in vitro results indicated that the PL-PVA/DOP-CaCO3 hydrogel had good biocompatibility and inflammatory activity and could promote fibroblast proliferation and migration. In vivo experiments demonstrated that the INS@PL-PVA/DOP-CaCO3 hydrogel can significantly promote wound healing in diabetic rats by glucose-responsive regulation of hyperglycemia, inhibiting inflammation, improving angiogenesis, and accelerating the secretion of endothelial cells and proliferation of fibroblasts on wound tissues. The results bring new insights into the field of glucose-responsive hydrogels, showing their potential as drug delivery systems of macromolecular therapeutics to treat diabetic skin wounds.

Synthetic phenolic antioxidants PG (propyl gallate), TBHQ (tert-butyl hydroquinone), BHA (butyl hydroxyanisole) are commonly used additives in edible oils, but excessive residues may pose health risks. Conventional detection methods suffer from high organic solvent consumption, complex pretreatment procedures, and environmental concerns. To address these limitations, this study developed a rapid, green and efficient analytical method combining deep eutectic solvent (DES)-based liquid-liquid microextraction (LLME) with high-performance liquid chromatography (HPLC) for the rapid determination of phenolic antioxidants in edible oils. Using an optimized hydrophilic DES system composed of choline chloride and 1,3-butanediol, (molar ratio 1:2), along with simplified extraction conditions (780 μL DES, 11 s vortex at room temperature), efficient extraction and enrichment of the target analytes were achieved. The method demonstrated excellent performance specifically for PG and TBHQ, exhibiting a wide linear range (1-400 μg/mL, R² > 0.9970), low limits of detection (0.12-0.28 μg/mL), satisfactory spiked recoveries (90.2 %-113 %), and good intra-day and inter-day precision (RSD < 5.0 %). Analysis of real samples demonstrated that the proposed method is highly sensitive, operationally simple, and environmentally friendly for the analysis of PG and TBHQ, offering a promising alternative for rapid monitoring and food safety regulation of these two antioxidants in edible oils. It should be noted that the current hydrophilic DES system did not achieve optimal extraction efficiency for BHA, highlighting the need for further optimization toward a comprehensive multi-analyte approach.

A nanocomposite electrocatalyst with three-dimensional coordinate axis shape was delicately prepared by a two-step pyrolysis of Zn/Fe zeolite imidazole precursor coated with phenolic resin. The first calcination step in a tubular furnace filled with Ar at 900 degrees C produced Fe3C embedded N doped carbon (NC) protected by a layer of NC derived from phenolic resin. Then, the obtained Fe3C/NC@NC was calcined in a muffle furnace with an air atmosphere at 350 degrees C to transform partial Fe3C into iron oxides and generate the final catalyst of FexOy-Fe3C/NC@NC. Through adjusting calcination time of the second step, the mass ratio between FexOy and Fe3C in FexOy-Fe3C/NC@NC was precisely regulated, so that a good balance between conductivity and catalytic performance could be achieved. Moreover, FexOy-Fe3C/NC@NC possessing a porous structure with a large specific surface area can facilitate the charge/mass transfer of oxygen intermediates and promote their contact with the catalytic centers. FexOy-Fe3C/NC@NC obtained a small potential difference (0.713 V) between oxygen reduction and oxygen evolution reactions due to the synergistic catalytic effect between Fe2O3 and Fe3C, which was verified by both experimental and density functional theory results. The liquid Zn-air battery with FexOy-Fe3C/NC@NC as the catalyst cathode obtained an open circuit voltage of 1.528 V, a power density of 199.37 mW cm(-2), a specific capacity of 850.4 mAh g(-1), and good stability of similar to 600 h. The electrocatalyst was also demonstrated to be applicable in solid button batteries.

This study investigates the effects Dioscorea opposita polysaccharides (DOP) on insulin resistance, lipid metabolism, oxidative stress, and intestine microbiota in high-fat-diet and streptozotocin induced type 2 diabetes (T2DM) rats. Low dose (DOP-L, 200 mg/kg BW), high dose (DOP-H, 400 mg/kg BW) and D. opposita powder (DO, 400 mg/kg BW) were oral-administrated to T2DM rats. After 6 weeks of treatment, supplementation of DOP-H and DO improved body weight and glucose/lipid metabolism-related indicators, including glucagon-like peptide 1, total cholesterol and high-density lipoprotein cholesterol. DOP-H and DO suppressed liver oxidative stress through increasing the level of superoxide dismutase, catalase, glutathione and reducing malondialdehyde. DOP attenuated the pathological change in liver, such as hepatic steatosis, and thus improved the liver function. Furthermore, the anti-diabetic effects of DOP was correlated with alterations of the gut microbiota, including an increase in Firmicutes and Bacteroidetes and a decrease in Actinobacteria and Proteobacteria, which promoted a healthier gut environment. Further analysis of short-chain fatty acids and metabolites provided evidences of DOP's regulatory effects on cecal contents in T2DM rats. Therefore, DOP-H present decent effects on T2DM, suggesting that DOP can ameliorate the insulin resistance and restore blood lipid level of T2DM rats with high-fat-diet by regulating intestinal microbiota.

A novel dual-monomer magnetic molecularly imprinted polymer (D/L-MMIP) was developed for the selective extraction of luteolin (Lut) by synergistically combining covalent and non-covalent imprinting strategies. The D/L-MMIP was synthesized via free radical polymerization using phenylboronic acid-functionalized Fe3O4 nanoparticles (covalent monomer) and acrylamide (non-covalent monomer). Comprehensive characterization (FT-IR, XRD, SEM, VSM) verified the successful fabrication of D/L-MMIP, confirming its molecularly imprinted structure, spherical morphology, and strong magnetic responsiveness, with Fe3O4 enabling rapid separation (<30 s). The adsorption kinetics reached equilibrium within 120 min at room temperature. Adsorption studies demonstrated pseudo-second-order kinetics (R-2 > 0.99) and Langmuir isotherm behavior (R-2 > 0.99), yielding a maximum adsorption capacity (q(max)) of 15.9 mgg(-1), which was significantly higher than those of non-imprinted (D/L-MNIP, 7.3 mgg(-1)) and single-monomer MIPs (S/L-MMIP: 4.2 mgg(-1); S/L-CMMIP: 7.4 mgg(-1)). The D/L-MMIP exhibited exceptional selectivity for Lut (imprinting factor = 4.76) over structurally similar flavonoids (quercetin, rutin, hyperoside), with selectivity coefficients (alpha) of 6.15, 2.64, and 1.53, respectively. The pH-responsive boronic ester bond facilitated efficient template release under acidic conditions (pH similar to 2.1), while the material maintained 83.5 % of its adsorption capacity after five regeneration cycles. When applied to Lonicera japonica Thunb. extracts, D/L-MMIP enrichment improved Lut purity from 36 % to 60 % (as determined by HPCE), demonstrating its practical applicability. This study provides a sustainable and efficient strategy for the selective separation of bioactive compounds, leveraging the complementary advantages of covalent and non-covalent interactions for enhanced molecular recognition.

A water stable cyclodextrin MOF (Cu-SD) was synthesized with γ-cyclodextrin derivative as organic ligand and Cu2+ as metal center to co-crystallizely load glycyrrhizic acid (GL) and glycyrrhetinic acid (GA). Cu-SD has a high drug loading capacity for GL (499.91μg/mg) and GA (112.37μg/mg), and the drug-loaded materials had a controlled release in different meadiums. In addition, Cu-SD and its drug loaded materials demonstrated better inhibiting α-glucosidase activity than the control drug acarbose. Furthermore, Cu-SD presented excellent antibacterial activity, and the antibacterial activity was significantly enhanced after GA and GL being encapsulated by Cu-SD. Moreover, both free and drug-loaded materials had good anti-inflammatory activities, and the anti-inflammatory effects of GL@Cu-SD and GA@Cu-SD were superior to those of their corresponding free drugs. Cu-SD, GL@Cu-SD and GA@Cu-SD demonstrated good biocompatibility and were applied to treat the wounds of diabetic rats. The experimental results showed that GL@Cu-SD and GA@Cu-SD had good promoting effects on the recovery of chronic diabetic wounds by suppressing wound inflammation.