Buffalo milk is nutritionally rich but vulnerable to adulteration, posing challenges to food safety. This study analyzes buffalo milk, soybean milk, Holstein milk, and five common additives-ammonium chloride, urea, sodium bicarbonate, sodium citrate, and sucrose-using Raman spectroscopy. Six spectral preprocessing methods were systematically evaluated to enhance model performance. For qualitative detection, PLS-DA models combined with Multiplicative Scatter Correction (MSC) preprocessing achieved excellent classification accuracy (up to 100 %) for pure buffalo milk, water, and soybean milk adulteration. For quantitative analysis, both PLS and MSC-CNN regression models were developed. The MSC-CNN model achieved high predictive performance for sodium bicarbonate (R-2 = 0.97) and sodium citrate (R-2 = 0.93), with RMSEP <5 % of full scale. Detection limits were as low as 17.4 mg/kg for sodium bicarbonate and 20.9 mg/kg for sodium citrate, meeting practical sensitivity requirements. Compared with existing PLS-based methods, our approach improved predictive accuracy and expanded low-concentration detection. The proposed Raman-deep learning strategy offers a rapid, accurate, and non-destructive solution for milk adulteration monitoring and quality control.

Spermatogonial stem cells (SSCs) serve as the foundation of spermatogenesis and play a critical role in livestock fertility and genetic improvement. However, maintaining these cells in long-term culture remains challenging, especially in pigs, where preserving both proliferative capacity and the undifferentiated state has proven difficult. To overcome this limitation, we investigated SB216763 (SB), a selective GSK-3β inhibitor reported to enhance proliferation in other stem cell types, for its potential effects on porcine spermatogonial cells (pSCs). In a 9-day concentration screening (0, 1.25, 2.5, and 5 μM SB), SB treatment significantly enhanced the proliferation of pSCs. It upregulated the proliferation-related gene PCNA and the antiapoptotic gene BCL2, while downregulating key apoptosis-related genes, including BAX and Caspase-3. It also promoted the expression of DAZL, UCHL1, NANOS2, and OCT4. Furthermore, over a 55-day period, treatment with 2.5 μM SB consistently supported higher proliferation rates, a larger proportion of DAZL-positive cells, and elevated expression of undifferentiation-associated markers (NANOS2, OCT4) compared with untreated controls at each passage. Overall, our results demonstrate that SB216763 not only promotes the proliferation of pSCs but also helps maintain their germline identity, offering a feasible strategy to improve the long-term in vitro culture of porcine spermatogonial cells.

As a major energy source in the metabolism of early embryos, lactate also participates in regulating zygotic gene expression and subsequently contributes to preimplantation embryonic development. Nevertheless, the influence of lactate on embryonic development in bovine cloned embryos remains elusive. For this reason, this research explored the differences in metabolic pathways between in vitro fertilization (IVF) embryos and somatic cell nuclear transfer (SCNT) embryos during zygotic genome activation (ZGA) using Smart-seq and observed changes in SCNT embryo development in response to lactate supplementation. Weighted gene coexpression network analysis (WGCNA) revealed that LDHA functions as a hub gene that significantly influences the gene expression profile of 8-cell SCNT embryos. Compared with those of IVF embryos, SCNT embryos showed lower LDHA levels and lactate contents. Lactate supplementation was found to increase the developmental potential and blastocyst quality of SCNT embryos. Furthermore, the addition of lactate significantly increased the immunofluorescence intensity of both Pan Kla and H3K18la as well as the expression levels of the zygotic genes ZSCAN5B and SUPT4H1 in SCNT embryos. These results indicate that lactate deficiency leading to the downregulation of histone lactylation may be an important factor affecting the in vitro development of SCNT embryos.

Background: Buffaloes are considered an indispensable genetic resource for dairy production. However, improvements in lactation performance have been relatively limited. Advances in sequencing technology, combined with genome-wide association studies, have facilitated the breeding of high-quality buffalo. Methods: We conducted an integrated analysis of genomic sequencing data from 120 water buffalo, the high-quality water buffalo genome assembly designated as UOA_WB_1, and milk production traits, including 305-day milk yield (MY), peak milk yield (PM), total protein yield (PY), protein percentage (PP), fat percentage (FP), and total milk fat yield (FY). Results: The results identified 56 significant SNPs, and based on these markers, 54 candidate genes were selected. These candidate genes were significantly enriched in lactation-related pathways, such as the cAMP signaling pathway (ABCC4), TGF-β signaling pathway (LEFTY2), Wnt signaling pathway (CAMK2D), and metabolic pathways (DGAT1). Conclusions: These candidate genes (e.g., ABCC4, LEFTY2, CAMK2D, DGAT1) provide a substantial theoretical foundation for molecular breeding to enhance milk production in buffaloes.

Buffaloes are a vital genetic resource for dairy production, yet advancements in improving milk production have been somewhat limited. In this study, we performed an integrated analysis of genomic sequencing data from 78 water buffaloes and their milk production traits, with a focus on 305-day milk yield (MY). Leveraging advancements in sequencing technology alongside genome-wide association study (GWAS) methods such as cBLUP, GMATs, and BayesR, we aimed to identify genetic factors that could facilitate the breeding of high-quality buffaloes. Our analysis revealed two significant SNPs associated with milk production traits. Based on these markers, four candidate genes were identified within the surrounding genomic regions. These genes showed significant enrichment in lactation-related pathways, including the prolactin signaling pathway (mitogen-activated protein kinase 10, MAPK10), IL-17 signaling pathway (MAPK10), MAPK signaling pathway (MAPK10), and adipocytokine signaling pathway (MAPK10). The identification of these candidate genes, particularly MAPK10, provides a robust theoretical basis for molecular breeding strategies aimed at enhancing milk production in buffaloes. This work paves the way for more targeted and effective breeding programs in the future.

Porcine reproductive and respiratory syndrome virus (PRRSV) infection has inflicted devastating impacts on the global swine industry, while current vaccines provide limited protection against this disease. Mogroside V (MV), a triterpenoid compound derived from Siraitia grosvenorii, exhibits diverse biological activities including antioxidant, anti-inflammatory, and anti-cancer properties, with the capacity to scavenge free radicals and mitigate oxidative stress. In this study, MV was administered to PRRSV-infected cells via three distinct treatment modalities. Our findings demonstrate that MV effectively blocks or suppresses infections caused by diverse PRRSV subtypes in porcine alveolar macrophages (PAMs) and Marc-145 cells. MV exhibited significant dose-dependent antiviral efficacy, with viral titers and mRNA expression inhibited by over 90% at a concentration of 400 μM. Comparative analysis further revealed substantial variations in antiviral efficacy among the different treatment protocols. Notably, PRRSV employs immune evasion mechanisms to suppress host innate immunity. MV not only directly inhibited PRRSV replication but also significantly upregulated the gene expression of immunomodulatory cytokines (IL-1, IL-2, IL-8, IL-18; P < 0.05), suggesting a dual mechanism of antiviral action. These findings underscore the antiviral bioactivity of MV and highlight its potential as a novel therapeutic candidate for PRRSV intervention.

In vitro maturation (IVM) of oocytes is pivotal for successful embryo production. Cumulus cells (CCs) contribute to oocyte maturation through the secretion of hormones and nutrients, with proper autophagic activity being crucial for this process. However, the role of autophagy in CCs remains underexplored. Siraitia grosvenorii extract Mogroside III (MIII), known for its antioxidant properties, has yet to be extensively studied for its impact on bovine oocyte IVM and its potential regulatory effects on autophagy. This study assessed the influence of MIII on autophagic activity in CCs and its subsequent effects on oocyte developmental potential. The results demonstrated that MIII enhanced bovine oocyte IVM, promoted CC expansion, and supported embryonic development. Transcriptomic analysis indicated that MIII upregulated the expression of autophagy-related genes. In vitro experiments on CCs revealed that MIII increased LC3B protein levels, reduced SQSTM1 accumulation, and upregulated the gene expression of LC3, Beclin1, and ATG5. In co-culture systems, autophagy inhibition in CCs impaired oocyte IVM and embryonic development, but MIII alleviated these effects, restoring oocyte developmental capacity compromised by 3-MA-induced autophagy inhibition. Mechanistically, MIII facilitated the degradation of WT1 by upregulating LC3B, influencing CC differentiation, enhancing FSHR synthesis, and increasing estrogen and progesterone secretion. In conclusion, MIII enhances oocyte developmental potential by modulating autophagy in CCs.

ABSTRACTOocyte quality is crucial for determining the subsequent embryo developmental capacity and reproductive outcomes. However, aging is detrimental to oocyte quality. Previous studies have demonstrated that soy isoflavones have positive effects on the reproductive performance of female pigs. Equol, the primary metabolite of soy isoflavones, is renowned for its antioxidant properties and its ability to scavenge reactive oxygen species (ROS). However, the potential role of equol in reversing aging‐mediated oocyte decline has not yet been elucidated. In this study, we treated the porcine oocytes with different concentrations of equol (2.5, 5 and 10 μM) during prolonged in vitro culture. Our findings showed that aging led to decreased embryonic developmental capacity, indicating the decline of oocyte quality. We further found that aging disrupted spindle assembly and chromosome arrangement, impaired actin polymerisation and reduced mitochondrial activity and function. Moreover, aging increased ROS levels; thereafter, DNA damage and apoptosis was induced in the porcine oocytes. Interestingly, treatment with 2.5 μM equol during the aging process significantly mitigated the above‐mentioned defective parameters in porcine oocytes and finally improved embryo development rates. Collectively, these results imply that equol has potential benefits in attenuating the aging‐mediated defects on porcine oocytes.

Polycystic ovary syndrome (PCOS), a complex endocrine-metabolic disorder characterized by hyperandrogenism, polycystic ovarian morphology, and ovulatory dysfunction, is often associated with insulin resistance. Mogroside-rich extract (MGE) from Siraitia grosvenorii possesses significant anti-inflammatory and antioxidant properties. However, its potential to restore intestinal microbial homeostasis and metabolic balance in PCOS and the underlying mechanisms remain unexplored. This study investigated MGE's protective effects and mechanisms in a letrozole-induced PCOS rat model. MGE administration significantly ameliorated estrous cycle irregularities, attenuated body weight gain, reduced cystic follicle formation in ovaries, and lowered serum testosterone and insulin levels. Integrated 16S rRNA sequencing and non-targeted metabolomics revealed that MGE enriched beneficial intestinal microbiota (Akkermansia, Parasutterella), associated with anti-inflammatory effects and metabolic improvement, while suppressing pro-inflammatory Corynebacterium. Notably, MGE partially reversed letrozole-induced alterations in colonic metabolites, restoring levels of anti-inflammatory metabolites like butyric acid and gamma-tocotrienol. Furthermore, MGE significantly reduced ovarian pro-inflammatory cytokines and downregulated the expression of p-NF-κB and NLRP3 proteins. Collectively, these findings demonstrate that MGE ameliorates PCOS symptoms by coordinately regulating ovarian inflammation via suppressing the NF-κB/NLRP3 pathway and restoring intestinal microbiota-metabolic axis balance, highlighting its therapeutic potential for PCOS.

Hypoxia benefits the proliferation and maintenance of animal spermatogonial cells; however, the underlying mechanism remains incompletely understood. This study aims to investigate the role and mechanism of the hypoxia-glycolysis-histone lactylation axis in the proliferation of buffalo spermatogonial cells (bSCs). bSCs were cultured under different oxygen concentrations to observe changes in cell proliferation. RNA-seq was used to analyze gene expression and signaling pathways. Changes in lactylation were monitored, and CUT&Tag-seq was utilized to determine the regulatory effects of lactylation on gene expression. The glycolytic pathway was regulated to validate the results of the bioinformatic analysis. Oxygen concentrations between 2.5% and 10% support the proliferation of bSCs, with 5% having the most pronounced effect. An amount of 5% oxygen significantly increased the proliferation and pluripotency of bSCs while also promoting glycolysis and lactylation. Inhibition of glycolysis eliminated the proliferative effects of hypoxia. By analyzing genes associated with the key lactylation site H3K18la using CUT&Tag technology, we found that it is closely linked to genes involved in the regulation of proliferation. After inhibition of HK-2 expression, cell proliferation, H3K18la expression, and the expression of these target genes were all suppressed. Hypoxia promotes the proliferation of bSCs via activation of glycolysis, leading to an increase in H3K18la and altered expression of its target genes.

Animals and humans are frequently infected by bacteria or exposed to bacterial derivatives in contaminated food, drinking water, or air, which significantly impacts their health. Among these bacterial sources, LPS (lipopolysaccharide) is the primary culprit. While it is widely known that LPS can cause liver inflammation and damage in animals, few studies have investigated this mechanism from the perspective of RNA editing. In this study, we administered LPS to mice via gavage to induce a liver injury model. We then used RNA editing omics approaches (RE-seq) to analyze RNA editing events potentially leading to liver inflammation following LPS administration, aiming to reveal the crucial role of RNA editing in LPS-induced processes. At the RNA editing level, we observed significant differences between the LPS group and the control (CON) group. Specifically, we identified 354 differentially edited genes, with 192 upregulated and 162 downregulated. These differentially edited genes were significantly enriched in pathways related to apoptosis, mTOR signaling, oxidative stress, and Nf-Kappa B signaling. By further integrating gene expression profiles and using a nine-quadrant analysis, we identified an important gene, Birc3, which showed significantly higher editing and expression levels in the LPS group. This gene is directly linked to liver inflammation and damage. The RNA editing of Birc3 represents a significant potential mechanism underlying LPS-induced liver damage, providing a novel approach for addressing animal and human health issues.

Polycystic Ovary Syndrome (PCOS) is a prevalent endocrine disorder characterized by metabolic dysfunction. This study investigated whether Mogroside V (MV) ameliorates hyperandrogenism-induced glycolytic dysfunction in testosterone (TES)-treated KGN cells. KGN cells treated with 150 µM TES exhibited significantly reduced viability, decreased lactate production, and increased pyruvate levels, which were reversed by 60 µM MV. Transcriptomic analysis revealed that TES dysregulated gene expression associated with alternative splicing (AS) and glycolytic pathways, while MV normalized glycolysis-related genes (LDHA, PKM) without affecting AS events. Although TES upregulated splicing factors HNRNPH3 and SRSF1, MV restored the expression of HNRNPH3 and SRSF1 without inducing aberrant splicing. Mechanistically, MV significantly reduced TES-induced hypermethylation of the LDHA promoter, thereby restoring LDHA mRNA and protein expression. MV mitigates PCOS-associated metabolic dysfunction primarily through LDHA promoter demethylation, independent of alternative splicing regulation. This study highlights MV as a natural compound with epigenetic regulatory potential for PCOS therapy.

Alternative splicing (AS) is a crucial mechanism in post-transcriptional regulation, contributing significantly to the diversity of the transcriptome and proteome. In this study, we performed a comprehensive AS profile in nine tissues obtained from Duroc (lean-type) and Luchuan (obese-type) pigs. Notably, 94,990 AS events from 14,393 genes were identified. Among these AS events, it was observed that 80% belonged to the skipped exon (SE) type. Functional enrichment analysis showed that genes with more than ten AS events were closely associated with tissue-specific functions. Additionally, the analysis of overlap between differentially alternative splicing genes (DSGs) and differentially expressed genes (DEGs) revealed the highest number of overlapped genes in the heart and skeletal muscle. The novelty of our study is that it identified and validated three genes (PYGM, MAPK11 and CAMK2B) in the glucagon signaling pathway, and their alternative splicing differences were highly significant across two pig breeds. In conclusion, our study offers novel insights into the molecular regulation of diverse tissue physiologies and the phenotypic differences between obese- and lean-type pigs, which are helpful for pig breeding.

The size of the initial primordial follicle pool in the ovary depends on primordial follicle formation, which determines the female reproductive lifespan. However, the molecular regulation of primordial follicle formation in chickens remains unclear. In this study, the left ovaries of chickens were collected at 2 d posthatch (dph), 5.5 dph, and 10.5 dph to examine the formation of primordial follicles. Single-cell mRNA sequencing (scRNA-seq) and spatial transcriptomic analysis were performed to explore the ovarian microenvironment and identify regulatory pathways involved in the formation of primordial follicles in chickens. Histomorphological analysis of chicken ovary tissues revealed the presence of germ cell cysts at 1 dph, which began to disintegrate at 2 dph. Primordial follicles appeared at 5.5 dph and continued to develop into larger-diameter follicles. scRNA-seq and spatial transcriptomic analysis revealed 24 cellular clusters involved in chicken primordial follicle formation. The metabolic pathway of steroid hormone synthesis was found in pregranulosa and pretheca cells. Histological analysis showed that chicken ovaries did not form primordial follicles after the inhibition of the steroid hormone synthesis pathway by simvastatin or tamoxifen. In addition, mRNA transcriptomic and bioinformatics analyses revealed that GREB1 was a downstream gene of the steroid hormone synthesis pathway during the formation of chicken primordial follicles. This study provides a valuable foundation for investigating primordial follicle formation in avian species and optimizing their reproductive performance.

RNA editing is a co-transcriptional/post-transcriptional modification that is mediated by the ADAR enzyme family. Profiling of RNA editing is very limited in pigs. In this study, we collated 3813 RNA-seq data from the public repositories across 23 tissues and carried out comprehensive profiling of RNA editing in pigs. In total, 127,927 A-to-I RNA-editing sites were detected. Our analysis showed that 98.2% of RNA-editing sites were located within repeat regions, primarily within the pig-specific SINE retrotransposon PRE-1/Pre0_SS elements. Subsequently, we focused on analyzing specific RNA-editing sites (SESs) in skeletal muscle tissues. Functional enrichment analyses suggested that they were enriched in signaling pathways associated with muscle cell differentiation, including DMD, MYOD1, and CAV1 genes. Furthermore, we discovered that RNA editing event in the 3 ' UTR of CFLAR mRNA influenced miR-708-5p binding in this region. In this study, the panoramic RNA-editing landscape of different tissues of pigs was systematically mapped, and RNA-editing sites and genes involved in muscle cell differentiation were identified. In summary, we identified modifications to pig RNA-editing sites and provided candidate targets for further validation.

Alternative splicing (AS) is a pivotal posttranscriptional regulatory mechanism that is involved in embryonic development. However, the roles of AS in specific developmental events, especially the zygotic genome activation (ZGA) of porcine early embryos, remain unclear. In this study, we demonstrated that alternative splicing events (ASEs) were most prevalent in mammalian embryos during ZGA and that skipped exons were the predominant splicing pattern. When splicing factor 3B subunit 1 (SF3B1) was disrupted by the inhibitor pladienolide B (PlaB), we observed that porcine embryos were markedly arrested at the 4-cell stage. Concurrently, the main ZGA genes, namely, DPPA2, EIF6, and SORD, underwent aberrant splicing indicative of the failure of ZGA. Moreover, embryonic metabolic homeostasis was significantly disrupted at the 4-cell stage by SF3B1 inhibition, resulting in increases in the LDHA/LDHB ratio and lactate levels. Interestingly, the levels of the histone lactylation modifications pan-Kla and H4K5la also increased. Our findings enhance our understanding of early mammalian embryonic development, reveal the crucial role of porcine early embryogenesis, and help to resolve reproductive difficulties related to embryonic development.

Heat stress (HS) is a stressor that negatively affect female reproduction. Specially, oocytes are very sensitive to HS. It has been demonstrated that some active compounds can protect oocyte from HS. We previously found that Mogroside V (MV), extracted from Siraitia grosvenorii (Luo Han Guo), can protect oocyte from many kinds of stresses. However, how MV alleviates HS-induced disruption of oocyte maturation remains unknown. In this study, we treated the HS-induced porcine oocytes with MV to examine their maturation and quality. Our findings demonstrate that MV can effectively alleviate HS-induced porcine oocyte abnormal cumulus cell expansion, decrease of first polar body extrusion rate, spindle assembly and chromosome separation abnormalities, indicating MV attenuates oocyte mature defects. We further observed that MV can effectively alleviate HS-induced cortical granule distribution abnormality and decrease of blastocyst formation rate after parthenogenesis activation. In addition, MV treatment reversed mitochondrial dysfunction and lipid droplet content decrease, reduced reactive oxygen species levels, early apoptosis and DNA damage in porcine oocytes after HS. Collectively, this study suggests that MV can effectively protect porcine oocytes from HS.

The demand for high-precision CRISPR/Cas9 systems in biomedicine is experiencing a notable upsurge. The editing system fdCas9 employs a dual-sgRNA strategy to enhance editing accuracy. However, the application of fdCas9 is constrained by the stringent requirement for two protospacer adjacent motifs (PAMs) of Cas9. Here, we devised an optimized editor, fRYdCas9, by merging FokI with the nearly PAM-less RYdCas9 variant, and two fRYdCas9 systems formed a dimer in a proper spacer length to accomplish DNA cleavage. In comparison to fdCas9, fRYdCas9 demonstrates a substantial increase in the number of editable genomic sites, approximately 330-fold, while maintaining a comparable level of editing efficiency. Through meticulous experimental validation, we determined that the optimal spacer length between two FokI guided by RYdCas9 is 16 base pairs. Moreover, fRYdCas9 exhibits a near PAM-less feature, along with no on-target motif preference via the library screening. Meanwhile, fRYdCas9 effectively addresses the potential risks of off-targets, as analyzed through whole genome sequencing (WGS). Mouse embryonic editing shows fRYdCas9 has robust editing capabilities. This study introduces a potentially beneficial alternative for accurate gene editing in therapeutic applications and fundamental research.

Skeletal muscle myogenesis hinges on gene regulation, meticulously orchestrated by molecular mechanisms. While the roles of transcription factors and non-coding RNAs in myogenesis are widely known, the contribution of RNA-binding proteins (RBPs) has remained unclear until now. Therefore, to investigate the functions of post-transcriptional regulators in myogenesis and uncover new functional RBPs regulating myogenesis, we employed CRISPR high-throughput RBP-KO (RBP-wide knockout) library screening. Through this approach, we successfully identified Eef1a1 as a novel regulatory factor in myogenesis. Using CRISPR knockout (CRISPRko) and CRISPR interference (CRISPRi) technologies, we successfully established cellular models for both CRISPRko and CRISPRi. Our findings demonstrated that Eef1a1 plays a crucial role in promoting proliferation in C2C12 myoblasts. Through siRNA inhibition and overexpression methods, we further elucidated the involvement of Eef1a1 in promoting proliferation and suppressing differentiation processes. RIP (RNA immunoprecipitation), miRNA pull-down, and Dual-luciferase reporter assays confirmed that miR-133a-3p targets Eef1a1. Co-transfection experiments indicated that miR-133a-3p can rescue the effect of Eef1a1 on C2C12 myoblasts. In summary, our study utilized CRISPR library high-throughput screening to unveil a novel RBP, Eef1a1, involved in regulating myogenesis. Eef1a1 promotes the proliferation of myoblasts while inhibiting the differentiation process. Additionally, it acts as an antagonist to miR-133a-3p, thus modulating the process of myogenesis.

为了探究茉莉花渣对广西黑山羊生长性能及血清生化指标、抗氧化和免疫指标的影响,试验将28只体重为24 kg左右的4月龄健康广西黑山羊随机分为两组(对照组和试验组),每组14只,对照组饲喂基础日粮,试验组饲喂用10％茉莉花渣替代基础日粮中6％玉米和4％豆粕的试验日粮,两组日粮营养水平相近,精粗比均为43:57,预试期7 d,正试验30 d;试验结束后,比较两组的生长性能(终末体重、总增重、平均日增重、平均日采食量和料重比)、经济效益(增重收益和毛收益)及血清生化指标[总蛋白(TP)、白蛋白(ALB)、球蛋白(GLB)水平,丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)和碱性磷酸酶(ALP)活性及总胆红素(TBIL)、直接胆红素(DBIL)、尿素氮(BUN)、葡萄糖(GLU)、三酰甘油(TG)、胆固醇(CHOL)、钙离子、镁离子、铁离子、磷离子、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)浓度]、抗氧化指标[总抗氧化能力(T-AOC),总超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)浓度]和免疫指标[免疫球蛋白A(IgA)、免疫球蛋白M(IgM)、免疫球蛋白G(IgG)、白细胞介素-1β(1L-1β)、白细胞介素-2(IL-2)、白细胞介素-4(IL-4)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、肿瘤坏死因子α(TNF-α)、γ干扰素(IFN-γ)、补体蛋白3(C3)、补体蛋白4(C4)、酸性磷酸酶(ACP)和溶菌酶(LZM)水平].结果表明:两组的终末体重、总增重、平均日增重、平均日采食量和料重比均差异不显著(P＞0.05),但试验组的增重收益和毛收益均高于对照组,分别提高了 11.81％和18.08％.试验组血清ALP活性、钙离子浓度、T-AOC和T-SOD活性及IgA、IgM、IgG、IL-4、C4水平显著高于对照组(P＜0.05),BUN和MDA浓度显著低于对照组(P＜0.05),其他血清生化指标、抗氧化指标和免疫指标在两组间差异不显著(P＞0.05).说明用10％茉莉花渣替代基础日粮中6％玉米和4％豆粕的试验日粮饲喂广西黑山羊,虽然对生长性能的影响不大,但是能明显提高养殖经济效益和抗氧化能力、免疫能力,且对血清生化指标没有负面影响.