To study the inhibitory effect of tryptanthrin on drug resistance to cisplatin chemotherapy for esophageal carcinoma and its mechanism,different treatments (blank control,cisplatin treatment,tryptanthrin treatment and cisplatin combined tryptanthrin treatment)of esophageal cancer Eca109 cells and cisplatin-resistant Eca109 cells (Eca109/cDDP)were performed on the basis of the purpose of experiments.Real-time quantitative PCR was used to detect the mRNA levels of multidrug resistance gene 1(MDR1)and glutathione-s-transferase-pi gene (GST-pi)in Eca109 and Eca109/cDDP cells.Western blot and/or immunofluorescence were used to detect the protein levels of MDR1 and GST-pi.The proliferation of cells was tested by cell counting kit (CCK-8).The results showed that tryptanthrin suppressed cell proliferation by inhibition of mRNA and protein levels of MDR1 and GST-pi in Eca109/cDDP cells. In summary,tryptanthrin can reverse the cisplatin resistance in Eca109/cDDP by its inhibition on MDR1 and GST-pi expression.It could be a potential adj uvant agent for chemotherapy.

本研究通过中国知网(CNKI)文献总库、维普资讯网、万方数据库检索,选取以针灸为主要治疗手段,主治脑卒中后总体认知功能障碍的相关疾病且有随机对照试验的文献为研究对象,剔除动物模型文献、综述性文献、重复发表的文献,进行Meta分析,以揭示针灸治疗认知功能障碍的治疗规律,为针灸治疗认知功能障碍的临床研究提供参考.

目的 为方志文献的挖掘、开发、利用提供检索工具. 方法 以福建地方志为信息源,构建福建方志中医药信息数据库,从信息的著录、描述、归类、存储、检索、查询诸方面,阐述中医药信息的组织方法和规范. 结果 方志记载的中医药信息内容分散,类目繁多,检索时应注重关键词检索,侧重类目检索. 结论 应根据方志书目的外表特征著录和信息归类,对著录过程中存在的不同问题采取不同的解决方法.

<正>地方志是指记录某一地区(行政区域)自然和社会情况的文献,简称方志[1]。其所记载资料丰富详细,包括地理、历史、人文、经济、政治等多项内容。地方志在资政、存史、教育等方面也发挥着重要作用,尤其在医学研究领域,地方志有着不可估

<正>地方志是指记录某一地区(行政区域)自然和社会情况的文献,简称方志[1]。其中,汇编于新中国成立以前的地方志称为旧志,而从宋朝到民国时期的地方志又统称为古代地方志。中华民族有