目的 调查分析大学新生军训期间焦虑发生状况及其影响因素,为做好早期干预提供依据.方法 采用ZUNG焦虑自评量表(SAS)对某院校新入学参加军训的2158人进行调查,按是否发生焦虑进行分组,对焦虑发生的影响焦因素进行单因素和多因素Logistic回归模型分析.结果 1574人发生焦虑,焦虑发生率为72.9％;单因素分析结果显示,焦虑组为城市生源、独生子女、贫困生、单亲家庭的比例明显高于健康组(P＜0.01);焦虑组适应性强、军训期间得到教官的支持指导、军训期间与教官互动的比例明显低于健康组(P＜0.01);多因素Logistic回归分析结果显示,在平衡了2组的生源地、是否独生子女、是否贫困生、是否单亲家庭及适应性强等因素后,军训期间得到教官的支持指导OR=0.439、军训期间与教官互动OR=0.416及适应性强OR=0.695是保护因素(P＜0.01).结论 大学新生在军训期间受到教官的关心、支持和指导,多与教官进行互动、有良好的适应性可降低焦虑的发生率.

目的 调查分析高职实习护生健康教育能力现状和影响因素,探讨提高其健康教育能力的对策.方法 采用自行设计的调查问卷对我院实习护生健康教育能力进行调查.结果 大多数护生知晓健康教育理论知识和技巧,能正确认识其在护理工作中的重要性和必要性,但临床运用能力不强,仅有9.66％的护生曾独立实施过健康教育.结论 护生健康教育能力培养是一个长期、系统的过程,需要多方面的密切配合.

目的:探讨NOX1对肺癌A549细胞凋亡和增殖的影响.方法:通过瞬时转染技术转染特异性的NOX1小干扰RNA(siRNA),按转染情况分为Mock组、NC组、FAM-NC-NOX1 siRNA组,转染12 h后采用Western blot检测NOX1蛋白的表达,通过荧光探针DCFH-DA检测细胞ROS水平,流式细胞仪检测细胞增殖活性和凋亡率.结果:siRNA转染12h以上,转染率稳定在80％以上,NOX1蛋白的表达量与Mock组相比下降(P＜0.05);与Mock组相比,FAM-NC-NOX1 siRNA组细胞在24、36、48 h的增殖活性均下降,ROS水平也下降(P＜0.05).结论:NOX1siRNA转染A549细胞能有效抑制细胞的增殖.

OBJECTIVE:To understand the hepatitis C virus infection status and related risk factors in drug users in Henan province during 2011-2015 and provide scientific evidence for the development of HCV infection prevention and control measures.METHODS:Cross-sectional questionnaire surveys were conducted among the drug users and blood samples were taken from them for HCV antibody detection during HIV sentinel surveillance period in Henan province from April to June during 2011-2015.RESULTS:The HCV infection rate in drug users increased from 3.70% in 2011 to 6.54% in 2015 (trend χ(2)=25.93, P<0.01). Multivariate analysis indicated that HCV infection related risk factors included age older (OR=1.23, 95% CI: 1.13-1.35, P=0.00), place of domicile (OR=3.45, 95%CI: 2.59-4.60, P=0.00), traditional drug user or mixed drug user (OR=1.46, 95%CI: 1.10-1.93, P=0.01; OR=1.82, 95%CI: 1.18-2.81, P=0.01), injecting drug user (OR=2.88, 95%CI: 2.45-3.39, P=0.00), commercial sex behavior in recent one year (OR=1.44, 95%CI: 1.20-1.72, P=0.00) and drug user in compulsory drug rehabilitation or those receiving methadone maintenance treatment (OR=1.79, 95% CI: 1.28-2.50, P=0.00; OR=1.74, 95% CI: 1.17-2.58, P=0.01).CONCLUSION:The HCV infection rate in drug users was higher in Henan and increased rapidly with years. In order to control HCV spread, it is necessary to strengthen the management of drug users and take effective intervention measures.