ObjectiveTo investigate the relationship between anxiety，depression， health promotion lifestyle and quality of life among empty⁃nest elderly with chronic disease in poor county of Hunan province，so as to provide the theoretical basis for the community nursing managers to make the management strategy of the rural empty-nest elderly with chronic diseases.MethodA total of 545 participants who were from 50 administrative villages in five townships of poor county （Anren county） of Hunan province，selected by stratified random sampling method，was surveyed by demographic questionnaire，the Geriatric Anxiety Inventory （GAI），the Short Form Geriatric Depression Scale（GDS⁃15），12⁃item Short Form Health Survey Version 2.0（SF⁃12v2） from March 2018 to August 2018.ResultsThe anxiety score of empty⁃nest elderly was 5.62±5.26，and the depression score was 3.84±2.17.The detection rates of anxiety and depression were 30.3% and 9.7%，respectively.Health promoting lifestyle score was 106.54±38.97 and quality of life score was 116.29±31.74.Correlation analysis showed that the depression score was negatively related with the total score of quality of life （r=-0.147，P<0.001），and also negatively correlated with health-promoting lifestyle（r=-0.216，P<0.001）.Anxiety and depression scores of empty⁃nest elderly with chronic disease were the main influencing factors of life of quality（F=16.859，P<0.001，R2=0.055）.ConclusionThe depression could directly affect the health promotion lifestyle and quality of life of empty-nest elderly with chronic disease in poor county of Hunan province.Community nursing managers should pay attention to the negative emotions of the empty-nest elderly with chronic diseases， and carry out targeted mental health education and lectures on chronic diseases to improve their health-promoting lifestyle and quality of life.

目的 了解湖南省贫困县农村空巢老人的互助养老意愿、需求及影响因素,为农村互助养老的发展定位及对策研究提供参考.方法 采用分层随机整群抽样的方法选择湖南省安仁县5个乡镇50个行政村的807名空巢老人,采用自制互助养老意愿及需求问卷、社会支持评定量表、健康促进生活方式量表进行调查.结果 272人(33.71％)选择愿意接受互助养老,互助养老需求评分为3.22±0.63.Logistic回归分析显示:贫困县农村空巢老人互助养老意愿受年龄、文化程度、自理能力、目前患慢性病数量、健康促进生活方式5个因素的影响(均P＜0.01).结论 贫困县农村空巢老人的互助养老意愿不强,对健康服务需求较高,高龄、文化程度高、自理能力差、患慢性病数量多、健康促进生活方式水平低的农村空巢老年人互助养老意愿相对较高.需提高农村空巢老人对互助养老的认知,以促进农村养老的健康发展.

目的 探讨葛根素抗内毒素血症小鼠血管内皮损伤的作用及其机制.方法 将18只BALB/C小鼠随机分为正常对照组,LPS组,葛根素组.ELISA法检测血清中肿瘤坏死因子-α(TNF-α),白细胞介素-1β(IL-1β),白细胞介素-6(IL-6),细胞间黏附分子-1(ICAM-1),血管细胞间黏附分子-1(VCAM-1)和E-选择素(E-selectin)的含量;左肺组织行HE染色进行病理学观察,右肺组织测湿干重比.结果 与正常对照组比,LPS组小鼠血清中炎症因子TNF-α和IL-1β以及黏附分子ICAM-1,VCAM-1和E-selectin水平明显升高,IL-6变化不明显,肺湿干重比增加,肺组织间隔增宽,炎症细胞浸润.与LPS组相比,葛根素组小鼠血清中炎症因子TNF-α和IL-β以及黏附分子ICAM-1,VCAM-1和E-selectin水平明显降低,IL-6变化不明显,肺湿干重比下降,肺组织间隔增宽程度降低,炎症细胞浸润减少.结论 葛根素预处理可明显减轻LPS所致内毒素血症小鼠血管内皮损伤,其机制可能与降低炎症因子和黏附分子水平有关.

In the present study, we aimed to investigate the effects of puerarin on the hyperpermeability of vascular endothelial cells induced by lipopolysaccharide (LPS) and its underlying mechanisms. Human umbilical vein endothelial cells (HUVECs) were pre-incubated with puerarin (25, 50, and 100 μM) for 1 h, and then exposed to LPS (1 μg/mL). The monolayer permeability of endothelial cells was assessed by measuring the paracellular flux of FITC-dextran 40,000 (FD40). The expression of vascular endothelial cadherin (VE-cadherin) in HUVECs was examined by Western blotting analysis. A total of 18 mice were randomly assigned into three groups as follows: control group, LPS group, and puerarin group. The pulmonary W/D ratio (wet-to-dry weight ratios) was calculated, and the lung morphology was examined. The levels of TNF-α and IL-1β in cell supernatant and mouse serum were determined by ELISA. Compared with the control group, LPS obviously increased the flux of FD40 and the monolayer permeability, raised the levels of TNF-α and IL-1β in cell supernatant, and reduced the VE-cadherin expression in HUVECs. However, puerarin (25, 50, and 100 μM) was able to relieve such LPS-induced increase in flux of FD40 and then reduce the hyperpermeability. Puerarin decreased the levels of TNF-α and IL-1β in cell supernatant and increased the VE-cadherin expression in HUVECs (P < 0.05). Moreover, LPS obviously increased the levels of TNF-α and IL-1β in mouse serum and elevated the pulmonary W/D ratios, resulting in lung injury. However, all of above-mentioned LPS-induced changes were improved by puerarin pre-treatment. Puerarin could alleviate LPS-induced hyperpermeability in endothelial cells via preventing downregulation of endothelial cadherin.