OBJECTIVE To establish an HPLC method for determination of Betamethasone in the blood and urine of human.METHODS Ethyl acetate was uesed as the solvent for liquid-liquid extraction and prednisone was used as the internal standard.Separation was achieved on Welchrom C18(250 mm×4.6 mm,5 μm) column using CH3OH-H2O(60∶40) as mobile phase.The detection wavelength was at 240 nm.RESULTS The linear limits of Betamethasone both ranged from 0.1 to 20 μg·mL-1(r2>0.999) in the blood and urine.The both limits of detection were 0.02 μg·mL-1.The mean recovery of this method ranged from 91% to 104%.The intra and inter day precision of Betam ethasone were no more than 4.6% and the mean extraction recovery was no less than 86.3%.CONCLUSION The method is simple,accurate,sensitive and suitable for determination of Betamethasone in the blood and urine.

Objective To establish a HPLC method for determination of 2,4-D butyl ester in blood and urine.Methods Compound was extracted from blood and urine using ethyl acetate.Separation was achieved on Zorbax SB-Aq column using methanol-water(60:40) as mobile phase.Results The calibration curve of 2,4-D butyl ester ranged from 0.10 μg/mL to 10.00 μg/mL in blood(r≥0.999 8) and 0.08 μg/mL to 8.00 μg/mL in urine(r≥0.999 5),respectively.The limits of detection for the analyte was 0.002 0 μg/mL and 0.001 8 μg/mL in blood and urine,respectively.The intra and inter day assay precision were no more than 4.5%.The mean recovery ranged from 94.5% to 104.5%.Conclusions The pre-treatment and HPLC analysis of 2,4-D butyl ester in blood and urine can be used for rapid diagnosis of 2,4-D butyl ester poisoning and forensic identification of 2,4-D butyl ester poisoning death.

CD86, one of the key costimulatory molecules, is not only involved in the initiation of T-cell immunity but also plays important roles in the development of cardiovascular diseases. The purpose of this study was to investigate the association between the CD86 polymorphism and the risk of coronary artery disease (CAD) in a Chinese population. We analyzed single-nucleotide polymorphism of CD86 +1057G/A (rs1129055) in 164 patients with CAD and 299 healthy controls by performing polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing assay. No significant association was observed in the genotype and allele frequencies of +1057G/A polymorphism between cases and controls, indicating that CD86 +1057G/A polymorphism may not be associated with CAD in the Chinese population.

OBJECTIVETo establish a new high performance liquid chromatography (HPLC) method for determining the concentration of cefazolin, cefradine, cefoperazone and cefotaxime in blood and urine, as well as to investigate its applicability.METHODSProtein in blood and urine was precipitated directly by acetonitrile with acetanilide was used as the internal standard using Agilent Zorbax SB-Aq column (250 mm x 4.6 mm, 5 microm). The mixed solvents of water (triethylamine 0.12%, acetic acid 0.12%) and acetonitrile were used as the mobile phase to separate cephalosporins using gradient elution method at 1 mL/min (flow rate) and 254 nm (detection wavelength).RESULTSThe working curve of four cephalosporins showed a good correlation (r = 0.9993), with the detection limit up to 0.01 microg/mL. The recovery rate was more than 81.2%.CONCLUSIONThis method is fast, easy and accurate. It is suitable for biological analysis of the 4 cephalosporins of the blood and urine in practical cases.

目的 建立大鼠脑中多巴胺浓度HPLC-FLD分析方法，并测定正常大鼠与氯胺酮成瘾大鼠脑中多巴胺浓度，用于研究多巴胺神经系统在氯胺酮成瘾机制中的作用。方法 本研究选用高氯酸沉淀蛋白的样品处理方法、Zorbax SB-aq C18柱（4.6mm×250mm，5μm）为分析柱、pH 3.5的缓冲液（0.01mol/L KH2PO4- H3PO4）-甲醇（90:10）为流动相，280nm和320nm分别为荧光激发波长和发射波长，并对所建方法的适用性进行考察和评价。同时，将健康雄性SD大鼠随机分成氯胺酮成瘾组和正常对照组，采用条件位置偏爱实验建立成瘾模型，运用确定的HPLC-FLD方法对大鼠脑中的多巴胺浓度进行测定，再进行统计学分析和比较。结果 所建分析方法，在0.020μg/mL~2.012μg/mL范围内线性关系良好，r2=0.9988，检测限（LOD）为10ng/mL，方法回收率为92.5%～103.0%，日内精密度RSD小于1.9%，日间精 密度RSD小于8.7%。成瘾组合正常对照组大鼠脑中多巴胺浓度分别为0.422μg/g和0.211μg/g，成瘾组大鼠全脑中多巴胺含量显著高于正常组大鼠（p＜0.05）。结论 本研究所建PHLC-FLD方法样品处理简单，灵敏度高，重现性好，适用于成瘾大鼠脑中多巴胺浓度变化的研究及药物成瘾机制、戒断治疗等研究中涉及的多巴胺浓度测定。与正常组大鼠相比，成瘾组大鼠大脑呈高多巴胺能状态，表明氯胺酮成瘾与大脑中神经递质多巴胺系统有关。