To investigate the effect of high-frequency electric welding (HFEW) on intestinal tissue healing, we performed end-to-end anastomosis experiments in New Zealand rabbits. Within one week post-surgery, animals exhibited normal vital signs, replaced necrotic tissue with healthy collagen, and showed improved tissue strength while inflammation decreased. By day 60, tissue pathology and function fully recovered, resembling normal tissue. Healing at the anastomotic site occurred in three phases: immediate adhesion, inflammation, and remodeling, with macrophages crucial for phagocytosis and regeneration of necrotic tissue. This study enhances understanding of HFEW’s healing mechanisms and supports further preclinical investigations.

The use of high-frequency electric welding technology for intestinal end-to-end anastomosis holds significant promise. Past studies have focused on in vitro, and the safety and efficacy of this technology is uncertain, severely limiting the clinical application of this technology. This study investigates the impact of compression pressure, energy dosage, and duration on anastomotic quality using a homemade anastomosis device in both in vitro and in vivo settings. Two hundred eighty intestines and 5 experimental pigs were used for in vitro and in vivo experiments, respectively. The in vitro experiments were conducted to study the effects of initial pressure (50–400 kpa), voltage (40–60 V), and time (10–20 s) on burst pressure, breaking strength, thermal damage, and histopathological microstructure of the anastomosis. Optimal parameters were then inlaid into a homemade anastomosis and used for in vivo experiments to study the postoperative porcine survival rate and the pathological structure of the tissues at the anastomosis and the characteristics of the collagen fibers. The anastomotic strength was highest when the compression pressure was 250 kPa, the voltage was 60 V, and the time was 15 s. The degree of thermal damage to the surrounding tissues was the lowest. The experimental pigs had no adverse reactions after the operation, and the survival rate was 100

Mesenchymal stem cell-derived exosomes (MSC-Exo) offer promising therapeutic potential for various refractory diseases, presenting a novel therapeutic strategy. However, their clinical application encounters several obstacles, including low natural secretion, uncontrolled biological functions and inherent heterogeneity. On the one hand, physical stimuli can mimic the microenvironment dynamics where MSC-Exo reside. These factors influence not only their secretion but also, significantly, their biological efficacy. Moreover, physical factors can also serve as techniques for engineering exosomes. Therefore, the realm of physical factors assumes a crucial role in modifying MSC-Exo, ultimately facilitating their clinical translation. This review focuses on the research progress in applying physical factors to MSC-Exo, encompassing ultrasound, electrical stimulation, light irradiation, intrinsic physical properties, ionizing radiation, magnetic field, mechanical forces and temperature. We also discuss the current status and potential of physical stimuli-affected MSC-Exo in clinical applications. Furthermore, we address the limitations of recent studies in this field. Based on this, this review provides novel insights to advance the refinement of MSC-Exo as a therapeutic approach in regenerative medicine.

外泌体是一种微小的膜性囊泡结构,是脂肪来源干细胞旁分泌的重要组成部分,可将信息物质传递至细胞或组织从而参与机体各种生物学过程,并在清除废弃物质、介导细胞信息传递、肿瘤治疗以及组织修复再生等方面发挥重要作用.外泌体可为毛发再生提供一种新型治疗策略.现就脂肪来源干细胞外泌体(exosomes from adipose-derived stem cells,ADSC-Exos)的相关生物特征作一综述,分析脱发产生的发病机理及其相关治疗措施,并对临床中使用外泌体治疗脱发及其毛发再生可能涉及的相关机制进行阐述.

ObjectiveThis study provided a systematic analysis of the trend in incidence and incidence-based mortality for cutaneous squamous cell carcinoma (cSCC) on the lips in the USA using demographic characteristics from the Surveillance, Epidemiology, and End Results (SEER) database.MethodsPatients diagnosed with cSCC on the lips between 2000 and 2019 from the 17 registries of the USA were identified. Incidence and incidence-based mortality rates were analyzed using SEER*Stat 8.4.0.1 software. This paper calculated incidence rates and incidence-based mortality rates by 100,000 person-years for sex, age, race, SEER registries, median household income ($/year), rural-urban distribution, and primary site. The annual percent changes (APC) in incidence and incidence-based mortality rates were then calculated using joinpoint regression software.ResultsAmong 8,625 patients diagnosed with cSCC on the lips from 2000 to 2019, men (74.67%), white (95.21%), and 60–79 years old were the most common population, and 3,869 deaths from cSCC on the lips occurred. The overall incidence of cSCC on the lips was 0.516 per 100,000 person-years. cSCC on the lip incidence rates were highest among men, white, and patients aged 60–79 years old. cSCC on the lip incidence rates decreased by 3.210%/year over the study period. The incidence of cSCC on the lips has been decreasing in all sexes, ages, high- or low-income households, and urban or rural patients. The overall incidence-based mortality rate of cSCC on the lips during 2000–2019 was 0.235 per 100,000 person-years. cSCC on the lip incidence-based mortality rates were highest among men, whites, and people older than 80 years old. cSCC on the lip incidence-based mortality increased by 4.975%/year over the study period. cSCC on the lip incidence-based mortality rates increased for all sexes, races, ages, primary sites, high- or low-income households, and urban or rural patients during the study period.ConclusionAmong patients in the USA diagnosed with cSCC on the lips from 2000 to 2019, the overall incidence decreased by 3.210% annually, and incidence-based mortality increased by 4.975%/year. These findings update and supplement the epidemiological information of cSCC on the lips in the USA.

With the development of science and technology, nanomaterials, especially superparamagnetic iron oxide nanoparticles (SPIONs), aroused great interests of researcher due to their special performance. SPIONs below 10 nm had good superparamagnetism, excellent magnetic field response which widely applied in biomedical field. Researchers had developed a variety of methods for synthesizing SPIONs. However, nanoscale magnetic materials tended to agglomerate and oxidized due to their high surface energy and high surface metal chemical activity; as a result, the magnetic properties and dispersibility of the SPIONs were reduced. Moreover, naked SPIONs possessed poor biocompatibility. Hence, it was necessary for surface modification of SPIONs in practical applications. In fact, SPIONs were usually surface coated by various materials to maintain their properties including non-polymeric organic stabilizers, polymeric stabilizers, and inorganic molecules. SPIONs had good dispersibility and biocompatibility after surface treatment. To date, SPIONs had been widely used in biomedical fields including magnetron therapy, magnetic hyperthermia, MRI, biological separation, and catalysis. Neurological conditions were the second leading cause of death globally, accounting for 9 million deaths per year. With increasing life stress, the increasing incidence of brain diseases imposes a severe economic burden on society and families. SPIONs owned excellent magnetism which responded superiorly to magnetic fields. In the presence of an external magnetic field, the magnetic nanoparticles are controlled by the magnetic field. More and more attention was fixed on SPIONs in nervous system. In review, recent advances of SPIONs and its applications in neuroscience under external magnetic field were included.