Functional open reading frames (ORFs) originating from long noncoding RNAs (lncRNAs) have historically been overlooked, in part due to the technical challenges associated with their identification and detection in plants. Here, we presented a comprehensive analysis by integrating 119 Ribo-seq (ribosome profiling sequencing) datasets from multiple rice tissues and developmental stages, and examined the biological potential of micropeptides encoded by ORFs translated from lncRNAs (lncORFs). 8,253 lncORFs were identified in the rice (Oryza sativa) genome. We confirmed the existence of 776 lncORF micropeptides by analyzing 1,188 mass spectrometry datasets and experimentally validated a set of lncORF-encoded micropeptides. Evolutionary and variant-association analysis pointed to the contributions of lncORF micropeptides to agronomic traits. Specifically, gain/loss-of-function experiments showed that the micropeptides regulate grain yield by affecting the number of small vascular bundles. Our findings expand the known functional scope of lncRNAs and provide valuable resources for rice improvement.

Purpose:Elderly patients are susceptible to dose-dependent perioperative hypotension caused by propofol. This study investigates whether general anesthesia induction with ciprofol reduces subsequent propofol maintenance requirements and improves hemodynamic stability compared to propofol induction in elderly patients undergoing total knee arthroplasty (TKA). Participants and Methods:In this prospective, double-blind, randomized controlled trial, 52 elderly participants (≥ 65 years, ASA I-III) undergoing TKA were randomly allocated to receive propofol (2 mg/kg) or ciprofol (0.4 mg/kg) for anesthesia induction. All participants received standardized anesthesia protocol for maintenance. The primary outcome was the average propofol infusion rate. Secondary outcomes included intraoperative norepinephrine utilization, mean arterial pressure (MAP) fluctuations, time to extubation, and postoperative modified Aldrete scores. Data were analyzed using t-tests, Wilcoxon rank-sum, or Chi-square/Fisher's exact tests as appropriate. Results:The mean age was 70.62 ± 3.86 years in the ciprofol group and 69.08 ± 3.52 years in the propofol group with balanced sex distribution. During anesthesia maintenance, the ciprofol group required a significantly lower propofol infusion rate than the propofol group (2.70 ± 0.75 mg/kg/h vs. 4.18 ± 1.66 mg/kg/h; mean difference, -1.48 mg/kg/h; [95% confidence interval, -2.19 to -0.77], P = 0.009). Additionally, the ciprofol group presented with a lower norepinephrine utilization rate (46.2% vs. 84.6%, P = 0.004) and a reduced median norepinephrine infusion rate (0 [interquartile range (IQR), 0-0.05] μg/kg/min vs. 0.05 [IQR, 0.01-0.08] μg/kg/min, P = 0.030). Norepinephrine initiation was significantly delayed in the ciprofol group (P = 0.047). No significant differences were observed in MAP variability or postoperative modified Aldrete scores. Conclusion:In relatively healthy elderly patients undergoing total knee arthroplasty, anesthesia induction with ciprofol is associated with reduced propofol maintenance requirements and decreased intraoperative norepinephrine utilization. Ciprofol may represent a promising alternative for anesthesia induction in this population, although further multicenter validation is warranted.

BACKGROUND:The liver is essential for coagulation-anticoagulation balance. Ischemia-free liver transplantation (IFLT) has been proven to prevent ischemia-reperfusion injury (IRI) and improve postoperative recovery. In this study, we explored protective effects of IFLT on the coagulation system. MATERIALS AND METHODS:Sixty-five liver transplant patients were enrolled in this post hoc analysis of the IFLT-DBD (ischemia-free transplantation of livers from donors after brain death) trial. Data of blood loss, blood product transfusion, intraoperative conventional coagulation tests, and rapid thromboelastography were evaluated. Transcriptome analysis was performed, and liver tissue specimens were collected for experimental validation. RESULTS:Total blood loss was significantly lower in the IFLT group than in the conventional liver transplantation (CLT) group [1765.0 (565.0-2965.0) vs 2600.0 (595.0-4605.0) mL, P<0.001]. The IFLT group had fewer red blood cells (RBC), fresh frozen plasma (FFP), and platelet (PLT) transfusions than the CLT group [RBC: 4 (2-6) vs 7.25 (2.25-8) units, P = 0.026; FFP: 600 (0-600) vs 600 (400-987.5) ml, P = 0.031; PLT: 6(18.8%) vs 13(39.4%), P = 0.026]. Bulk transcriptome analysis showed that hepatocyte nuclear factor 4 alpha (HNF4α) expression was higher in the IFLT group. Real-time PCR revealed higher mRNA expression of HNF4α, factor II, and V in the IFLT group. Western blotting showed higher HNF4α protein expression in the IFLT group. CONCLUSION:Adopting IFLT significantly decreased intraoperative hemorrhage and blood product transfusion. IFLT facilitates the restoration of intraoperative coagulation homeostasis by preserving the HNF4α-mediated regulation of hepatic coagulation factor synthesis.

Lnc-eRNAs are long non-coding RNAs (lncRNAs) whose transcription initiation sites overlap with enhancers, but whether lnc-eRNAs exist in plants and whether they affect disease resistance remain unknown. Here, we identified lnc-eRNAs in rice (Oryza sativa L.) using transcriptome and chromatin accessibility data and characterized one lnc-eRNA, Xanthomonas Susceptible Enhancer RNA1 (XSER1), which was quickly activated under pathogen infection. XSER1 knockdown conferred resistance to bacterial and fungal diseases caused by Xanthomonas and Magnaporthe oryzae, respectively, showing broad-spectrum disease resistance. We found that XSER1 binds to the transcription factor basic helix-loop-helix 94 (OsbHLH94) and promotes chromatin looping to shape chromatin accessibility and rewire XSER1 activation, as well as affecting the expression of the adjacent histone H3 lysine 4 (H3K4) demethylase gene JUMONJI 707 (OsJMJ707), thus conferring resistance to pathogens. XSER1 abundance is very low under normal conditions but increases substantially within hours of pathogen infection. Knocking out XSER1 did not cause developmental defects but enhanced plant defenses, revealing its potential for breeding pathogen-resistant plants.

OBJECTIVES:The purpose of this study was to evaluate the efficacy of Efgartigimod (EFG) in anti-N-methyl-d-aspartate receptor (anti-NMDAR) encephalitis patients during acute attacks. METHODS:A case-control study was designed to compare 26 anti-NMDAR encephalitis patients who were treated with EFG, and 15 patients with intravenous immunoglobulin (IVIG), and 23 patients with immunoadsorption with staphylococcal protein A column (SPA-IA) treatment. RESULTS:At baseline, no significant differences in mRS scores were observed among the EFG, IVIG, and SPA-IA groups of anti-NMDAR encephalitis patients. When compared with the IVIG group, patients treated with EFG had significantly decreased serum IgG levels. Compared with the SPA-IA group, EFG-treated patients had lower CSF anti-NMDAR antibody titers at admission (p = 0.039) and higher post-treatment IgG levels (p = 0.002). When compared with the IVIG group, SPA-IA patients had higher CASE scores (p = 0.022) and baseline IgG levels (p = 0.023). All groups improved the symptoms of anti-NMDAR encephalitis patients after treatment during acute attacks, with significant decreases in mRS and CASE scores from admission to discharge (p < 0.01). In the EFG and SPA-IA groups, there was a significant reduction in anti-NMDAR antibody titers in both CSF and serum (p < 0.01), while no remarkable decrease was found in the IVIG group. Additionally, serum IgG levels significantly decreased in both the EFG and SPA-IA groups post treatment and during the 1-month follow-up. By the 3-month of follow-up, IgG levels in the blood of both groups remained below the baseline levels. CONCLUSION:EFG could be an elegant alternative to both IVIG and SPA-IA therapies for anti-NMDAR encephalitis during acute attacks. It has a better effect on reducing antibody titers than IVIG and is comparable to SPA-IA therapy, and no serious adverse events were observed during infusion.

Introduction:Autoimmune glial fibrillary acidic protein astrocytopathy (A-GFAP-A) is an increasingly recognized neurological disorder with significant clinical management challenges, particularly in predicting the need for intensive care unit (ICU) admission. This study aimed to develop and validate predictive models to identify A-GFAP-A patients at increased risk for ICU admission. Methods:We retrospectively analyzed 107 patients (January 2021 - August 2024), randomly assigned to training and validation cohorts (7:3). Variable selection for model development was performed using random forest, least absolute shrinkage and selection operator (LASSO), and extreme gradient boosting (XGBoost). Logistic regression was used to construct a nomogram, and a decision tree was developed to facilitate rapid clinical decision-making. Model performance was assessed by area under the curve (AUC), calibration plots, and decision curve analysis (DCA). Results:Four key predictors of ICU admission were identified: Glasgow Coma Scale (GCS) score at admission, seizures, maximum body temperature, and C-reactive protein (CRP) levels. The nomogram demonstrated excellent predictive accuracy with AUCs of 0.923 (95% CI, 0.858-0.987) in training cohort, 0.922 (95% CI, 0.836-1.000) in validation cohort, and 0.93 (95% CI, 0.883-0.972) in bootstrap validation. The model showed excellent calibration, and DCA confirmed its clinical utility. The decision tree identified GCS <15, seizures, and temperature >39°C as the most relevant indicators for high-risk stratification. Discussion:This study presents the first validated nomogram and decision tree for ICU admission risk in A-GFAP-A, based on the largest reported cohort to date, providing a valuable tool for clinical decision-making and resource optimization.

The identification and genomic editing of defense-related genes to confer resistance to pathogens is an effective and promising strategy for use in crop breeding. However, resistance is often associated with growth inhibition, a phenomenon referred to as the "trade-off" effect, making enhancing resistance without sacrificing yield challenging. In this study, a novel strategy is presented to enhance broad-spectrum resistance in crops without yield loss by editing susceptibility lncRNAs. RESIS, a pathogen-induced lncRNA that acquired its function in the pathogen response during domestication, is identified. Upon pathogen invasion, RESIS is activated by effector-binding elements on its promoter and subsequently binds to NAA15 and NAA10, two core components of the NatA complex. RESIS enables NAA10 to interact with NAA15 through a sequence that evolves in cultivated rice, enhancing the activity of the NatA complex in the N-terminal acetylation of proteins. RESIS knockout suppresses this process and increases translation during pathogen invasion, conferring resistance to both fungal and bacterial diseases without the growth inhibition typically associated with the direct knockout of the NatA complex. These findings highlight the potential of susceptibility lncRNAs as promising target loci for improving crop broad-spectrum disease resistance without detrimental effects on growth, offering significant prospects for practical applications.

Seed dormancy enables seeds to remain dormant until the environmental conditions are ideal for germination. Understanding the molecular mechanisms that underlie seed dormancy is essential for improving grain quality and preventing pre-harvest sprouting (PHS), a major challenge in global agriculture. Here, we address how long noncoding RNAs (lncRNAs) contribute to the regulation of seed dormancy in rice (Oryza sativa). We identified an lncRNA, VIVIpary, that is specifically expressed in embryos and is associated with shortened seed dormancy. VIVIpary exhibits higher expression in a PHS-sensitive variety, and its overexpression induces PHS, whereas its knockdown delays germination. Mechanistically, VIVIpary promotes the release of seed dormancy by regulating abscisic acid (ABA) signaling. VIVIpary serves as a spatial organizer that shapes chromatin architecture by directly binding to the chromatin adaptor protein OsMSI1 and enhancing its interaction with the histone deacetylase OsHDAC1, thereby reducing chromatin accessibility and fine-tuning ABA signaling. VIVIpary is differentially expressed between wild and cultivated rice, with higher expression in japonica rice, suggesting that it was a target of selection during rice domestication. Together, our findings reveal a domestication-associated lncRNA that modulates ABA signaling and chromatin architecture to regulate seed dormancy and PHS in rice, providing a potential target for improvement of rice agronomic traits.

AIMS:The aim of our study was to evaluate the efficacy of efgartigimod and intravenous immunoglobulin (IVIG) on AQP4-IgG-positive neuromyelitis optica spectrum disorder (NMOSD) patients during acute attacks. METHODS:A retrospective case-control study was designed to compare the clinical outcomes of 13 NMOSD patients treated with efgartigimod (at a dose of 20 mg/kg in the first and fifth day) and 20 NMOSD patients treated with IVIG (at 0.4 g/kg/day for 5 days). Follow-up outcome information for patients is documented at 6 months postdischarge. RESULTS:Compared with IVIG, efgartigimod could improve NMOSD patients' symptoms during acute attacks, the mean Expanded Disability Status Scale score was significantly improved from 3.0 at admission to 2.5 at discharge (P < .001). The serum IgG levels were obviously decreased in NMOSD patients treated with efgartigimod (P < .001). Additionally, AQP4-IgG titres in 5 NMOSD patients were found to turn negative after efgartigimod treatment. CONCLUSION:The efficacy of efgartigimod is comparable to IVIG therapy in improving acute symptoms of AQP4-IgG-positive NMOSD. Efgartigimod could be an elegant alternative to IVIG therapy, and no serious adverse events were observed during infusion.

Rice bacterial blight is a devastating disease worldwide, causing significant yield losses. Understanding how plants defend against microbial infection is critical for sustainable crop production. In this study, we show that ALEX1, a previously identified pathogen-induced long noncoding RNA, localizes to the nucleus and directly binds AUXIN RESPONSE FACTOR 3 (ARF3). We showed that ARF3 forms the condensates in the nucleus via its intrinsically disordered middle region (MR), and that these ARF3 condensates display solid-like properties. We further revealed that ALEX1 directly binds the MR of ARF3 to regulate ARF3 condensate dynamics and promote ARF3 homodimerization. The dispersed, dimeric form of ARF3, referred to as its functional phase state, enhances its ability to transcriptionally repress the expression of downstream target genes such as JAZ13, thereby modulating the jasmonic acid signaling pathway and enhancing pathogen resistance in rice. Collectively, this study reveals the role of a long noncoding RNA in regulating protein condensation and complex assembly, thus contributing to plant pathogen resistance.

BACKGROUND:Non-HIV associated cryptococcal meningitis (CM) patients with negative cerebrospinal fluid (CSF) culture lack validated biomarkers for outcome assessment. Baseline CSF cryptococcal count via India ink staining correlate with disease progression, but the role of cryptococcal clearance rate remains unclear in this population. METHODS:We retrospectively collected data from 594 non-HIV associated CM patients from the Third Affiliated Hospital of Sun Yat-sen University. 111 non-HIV associated CM patients with negative CSF culture were included to analyse. We focused on analyzing the association between cryptococcal clearance rate and outcome. RESULT:Baseline high log10 CSF cryptococcal count (OR: 2.939, 95%CI 1.574-5.488, p=0.001) and slow rate of cryptococcal clearance (OR: 92.228, 95%CI 6.453-1318.128, p=0.001) were associated with unsuccessful outcomes at 2 weeks after admission. The baseline high log10 CSF cryptococcal count (OR: 1.520, 95%CI 1.045-2.211, p=0.029), slow rate of cryptococcal clearance (OR: 162.896, 95%CI 10.028-2646.031, p=0.001) and hearing impairment at baseline (OR: 8.683, 95%CI 1.294-58.291, p=0.026) were associated with unsuccessful outcomes at 10 weeks after admission. And hearing impairment at baseline (log-rank test, p=0.007), baseline log10 CSF cryptococcal count ≥1.52/ml (log-rank test, p=0.033) were associated with no clinical cure within 1 year after admission. CONCLUSION:Rate of cryptococcal clearance based on India ink staining may be a marker for short-term outcomes in non-HIV associated CM patients with negative CSF culture. Monitoring this marker may optimize clinical management in this challenging subgroup. Notably, its strengthened association at 10 weeks reflects sustained prognostic value, while the attenuated effect of baseline count suggests diminishing early impact.

BACKGROUND:Ischemia-free liver transplantation (IFLT) prevents ischemia-reperfusion injury (IRI) and reduces IRI-related complications. In this study, we explored the protective effects of IFLT on myocardial injury after noncardiac surgery (MINS) and postoperative pulmonary complications (PPCs). MATERIALS AND METHODS:Sixty-five patients who underwent liver transplantation were enrolled in this post hoc analysis of the IFLT-DBD trial. Intraoperative pulse indicator continuous cardiac output, pulmonary artery catheter, and blood gas parameters were prospectively collected. RESULTS:The incidence of MINS did not differ between the two groups (28.1% vs. 45.5%, P = 0.147), although the peak high-sensitive troponin level was significantly lower in the IFLT group than in the conventional liver transplantation (CLT) group (0.056 ± 0.007 vs. 0.088 ± 0.016 ng/mL, P = 0.036). The incidence of PPCs was lower in the IFLT group than in the CLT group (37.5% vs. 66.7%, P = 0.019). Multivariate analysis revealed that IFLT was an independent protective factor against PPC. The median duration of ventilation was significantly shorter in the IFLT group than in the CLT group [12.5 (3.5-21.5) vs. 18 (0-69.5) hours, P <0.001]. CONCLUSION:The use of IFLT is associated with lower peak troponin T level and lower incidence of PPC.

Introduction: Preservation fluid (PF) contaminations are common in conventional liver transplantation (CLT) and presumably originate from organ or PF exposures to the external environment in a non-strict sterile manner. Such exposures and PF contamination may be avoided in ischaemia-free liver transplantation (IFLT) because of the strict sterile surgical procedures. In this study, the authors evaluated the impact of IFLT on organ PF contamination. Methods: A post-hoc analysis using data from the first randomized controlled trial of IFLT was performed to compare the incidence, pathogenic spectrum of PF contamination, and incidence of early recipient infection between IFLT and CLT. Multivariable logistic regression was used to explore risk factors for PF contamination. Results: Of the 68 cases recruited in the trial, 64 were included in this post-hoc analysis. The incidence of culture-positive PF was 9.4% (3/32) in the IFLT group versus 78.1% (25/32) in the CLT group (P<0.001). Three microorganisms were isolated from PF in the IFLT group, while 43 were isolated in the CLT group. The recipient infection rate within postoperative day 14 was 3.1% (1/32) in the IFLT group vs 15.6% (5/32) in the CLT group, although this difference did not reach statistical significance (P=0.196). Multivariate analysis revealed that adopting IFLT is an independent protective factor for culture-positive PF. Conclusion: PF contamination is substantially decreased in IFLT, and IFLT application is an independent protective factor for PF contamination. Using rigorous sterile measures and effective antibiotic therapy during IFLT may decrease PF contamination.

Abstract Background Significant hemodynamic changes occur during liver transplantation, emphasizing the importance of precious and continuous monitoring of cardiac output, cardiac index, and other parameters. Although the monitoring of cardiac output by pulse indicator continuous cardiac output (PiCCO) was statistically homogeneous compared to the clinical gold standard pulmonary artery catheterization (PAC) in previous studies of liver transplantation, there are fewer statistical methods for the assessment of its conclusions, and a lack of comparisons of other hemodynamic parameters (e.g., SVRI, systemic vascular resistance index). Some studies have also concluded that the agreement between PiCCO and PAC is not good enough. Overall, there are no uniform conclusions regarding the agreement between PiCCO and PAC in previous studies. This study evaluates the agreement and trending ability of relevant hemodynamic parameters obtained with PiCCO compared to the clinical gold standard PAC from multiple perspectives, employing various statistical methods. Methods Fifty-two liver transplantation patients were included. Cardiac output (CO), cardiac index (CI), SVRI and stroke volume index (SVI) values were monitored at eight time points using both PiCCO and PAC. The results were analyzed by Bland-Altman analysis, Passing-bablok regression, intra-class correlation coefficient (ICC), 4-quadrant plot, polar plot, and trend interchangeability method (TIM). Results The Bland-Altman analysis revealed high percentage errors for PiCCO: 54.06% for CO, 52.70% for CI, 62.18% for SVRI, and 51.97% for SVI, indicating poor accuracy. While Passing-Bablok plots showed favorable agreement for SVRI overall and during various phases, the agreement for other parameters was less satisfactory. The ICC results confirmed good overall agreement between the two devices across most parameters, except for SVRI during the new liver phase, which showed poor agreement. Additionally, four-quadrant and polar plot analyses indicated that all agreement rate values fell below the clinically acceptable threshold of over 90%, and all angular deviation values exceeded ± 5°, demonstrating that PiCCO is unable to meet the acceptable trends. Using the TIM, the interchangeability rates were found to be quite low: 20% for CO and CI, 16% for SVRI, and 13% for SVI. Conclusions Our study revealed notable disparities in absolute values of CO, CI, SVRI and SVI between PiCCO and PAC in intraoperative liver transplant settings, notably during the neohepatic phase where errors were particularly pronounced. Consequently, these findings highlight the need for careful consideration of PiCCO’s advantages and disadvantages in liver transplantation scenarios, including its multiple parameters (such as the encompassing extravascular lung water index), against its limited correlation with PAC.

Colorectal cancer (CRC) stands among the top prevalent cancers worldwide and holds a prominent position as a major contributor to cancer-related mortality globally. Absent in melanoma 2 (AIM2), a constituent of the interferon-inducible hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats protein family, contributes to both cancer progression and inflammasome activation. Despite this understanding, the precise biological functions and molecular mechanisms governed by AIM2 in CRC remain elusive. Consequently, this study endeavors to assess AIM2's expression levels, explore its potential antitumor effects, elucidate associated cancer-related processes, and decipher the underlying signaling pathways in CRC. Our findings showed a reduced AIM2 expression in most CRC cell lines. Elevation of AIM2 levels suppressed CRC cell proliferation and migration, altered cell cycle by inhibiting G1/S transition, and induced cell apoptosis. Further research uncovered the participation of P38 mitogen-activated protein kinase (P38MAPK) in AIM2-mediated modulation of CRC cell apoptosis and proliferation. Altogether, our achievements distinctly underscored AIM2's antitumor role in CRC. AIM2 overexpression inhibited proliferation and migration and induced apoptosis of CRC cells via activating P38MAPK signaling pathway, indicating AIM2 as a prospective and novel therapeutic target for CRC.

Objective To analyze the clinical manifestations and histopathological features of cutaneous Mycobacterium marinum infections in order to improve the understanding, diagnosis and treatment of this disease. Methods We retrospectively analyzed the clinical manifestations, histopathological features, treatment and outcome of 12 cases of cutaneous Mycobacterium marinum infections diagnosed at the Fifth Affiliated Hospital of Sun Yat-Sen University from 2020 to 2023. Results The average age was 47.3 years. Seafood-induced trauma was a risk factor. Clinical presentations often included unilateral distribution of multiple chronic nodules and plaques on the upper limb, resembling sporotrichosis. Pathological findings included epidermal acanthosis, dermal non-specific inflammatory infiltration, and sometimes with tuberculosis-like granulomas. However, the positive rate of acid-fast staining was low. Nine patients received anti-infective therapy consisting of clarithromycin combined with rifampicin or moxifloxacin. The treatment regimen for other three patients was unknown. All patients experienced resolution of their skin lesions without serious adverse drug reactions, and there were no recurrences during 1-year follow-up. Conclusions Mycobacterium marinum infection should be suspected in patients, especially middle-aged and elderly individuals with a history of trauma or handling seafood, presenting with chronic nodules and plaques, along with pathological findings of tuberculosis-like granuloma or non-specific inflammatory infiltration. These patients can be treated with clarithromycin and rifampicin.

To predict prognosis in HIV-negative cryptococcal meningitis (CM) patients by developing and validating a machine learning (ML) model. This study involved 523 HIV-negative CM patients diagnosed between January 1, 1998, and August 31, 2022, by neurologists from 3 tertiary Chinese centers. Prognosis was evaluated at 10 weeks after the initiation of antifungal therapy. The final prediction model for HIV-negative CM patients comprised 8 variables: Cerebrospinal fluid (CSF) cryptococcal count, CSF white blood cell (WBC), altered mental status, hearing impairment, CSF chloride levels, CSF opening pressure (OP), aspartate aminotransferase levels at admission, and decreased rate of CSF cryptococcal count within 2 weeks after admission. The areas under the curve (AUCs) in the internal, temporal, and external validation sets were 0.87 (95

Objective: Burn bacteremia is related to immune barrier damage, but whether the level of circulating immune cells predicts outcomes in severe burns is still not clear. This study aimed to explore the predictive value of perioperative blood cells of the first surgery after burn for bacteremia and 90-day death.Methods: Data from severe burn patients treated at the First Affiliated Hospital of Sun Yat-sen University from 2011 to 2020 were retrospectively analyzed. Data on monocytes (M), lymphocytes (L), white blood cell-to-platelet ratio (WPR), neutrophil-to-lymphocyte ratio (NLR) in peripheral blood and changes in temperature (T-37) were collected at one day before(X0), the first day after (X1) and the third day after (X3) the primary surgery.Univariate and multivariate logistic regression were used to identify the in-dependent risk factors of bacteremia and death within 90 days, which were used to es-tablish the risk prediction models (xbac and x90d-m) in severely burned patients. Severe burn cases from two other burn centers were selected to verify the prediction models.Results: We analyzed 169 severe burn cases in the training dataset, with a 90-day mortality of 21.3% (36/169); 56 (33.1%) patients experienced burn bacteremia. Higher M0, WPR0, NLR0, NLR3, T3-37, triangle M (M0-M3) and lower M3, L3 were associated with higher risk of bacteremia (P < 0.05). Multivariate regression analysis showed that SOFA0, WPR0, M3, and T3-37 were independently associated with bacteremia. The prediction model for bacteremia Xbac = 0.1809 x SOFA0 + 6.532 x WPR0-1.171 x M3 + 0.6987 x T3-37-2.297. TBSAB, SOFA0, and triangle M (M0-M3) were independently correlated with 90-day mortality. The risk prediction model X90d-m= 0.055 x TBSAB + 0.301 xSOFA0 + 1.508 x triangle M 7.196. External validation suggested that the specificity, sensitivity and AUC of the prediction model Xbac was 90.7%, 62.5% and 0.797, respectively; of the prediction model X90d-m was 69.2%, 90.0% and 0.873, respectively.Conclusion: Peripheral M3, WPR0 and triangle M (M0-M3) during the primary surgery has reasonable predictive ability for bacteremia and 90-day mortality in severe burn patients, which could inform clinical antimicrobial judgment and prognostication.(c) 2022 Elsevier Ltd and ISBI. All rights reserved.