Picking up data needed by diagnosis from thousands and thousands of cases stored in case history and follow-up database system,hold a effective mining algorithm to realize data mining of disease treatment.In this study,discuss in detail the theory of data mining and the related practical method of constructing a rule of correlation depending on the mountain of data in the case history and follow-up database.Through Apriori algorithm analysis,constructed a highly purposive correlation rule among the data.Through discussion,see that a suitable Apriori algorithm for the construction of a correlation rule will not only increase the efficiency of data mining in case history and follow-up database,but also set foundation for building a disease treatment information database.

There are a large of information recorded in the hospital every day.Data mining technique is to find common character about a illness from thousands of case history in the case history random visiting database,and to offer the method of diagnosing this illness,or to build a having reference worth medical treatment pattern database with the effective information filtered by mining for some illness.Dependent mining,advances diagnoses efficiency of the hospital and offer an effective emergency measure for the paroxysmal illness.Detailed discuss mining theory and how to utilize mining technique to realize the method of getting worth medical treatment information from the case history random visiting database.Through using mining technique,there is more advance about the analysis and integration of the worth information in the medical treatment information database and change the phenomenon about data richness,absence settle in the case history random visiting database system of the hospital.Through discussion,see that the mining technique is not only to advance the application efficiency of the case history random visiting database system,but also to offer very worth information for control and prevention of the paroxysmal epidemic.

AIM:To explore the mechanism of 3-nitropropionic acid (3-NPA) preconditioning that induces cerebral ischemic tolerance in rats by affecting the expression of brain-type glucose transporters (GLUT1 and GLUT3) at mRNA and protein levels in cerebral tissues.METHODS: The male SD rats were used in the experiments and divided randomly into sham operation group (sham group, n=4), control group of 3-NPA preconditioning (3-NPA group, n=4), cerebral ischemia group (M group, n=16) and 3-NPA preconditioning group (IPC group, n=16). M group and IPC group were further divided into 4 subgroups according to the different reperfusion time(4 h, 12 h, 24 h and 48 h). All rats were killed at the corresponding time points. The cerebral tissues in the ischemic side (left) and coronal intermediate 1/3 of cortex were collected. The protein levels and mRNA expression of GLUT1 and GLUT3 were determined by Western blotting and RT-PCR. RESULTS: Compared with M group, the ischemic reperfusion and 3-NPA preconditioning induced the upregulation of GLUT1 and GLUT3 at protein levels with significant differences (F=5.848, P<0.05 and F=6.295, P<0.05, respectively), especially after ischemia-reperfusion for 48 h. The mRNA expression of GLUT1 in IPC group began to increase at 4 h, peaked at 48 h after reperfusion, with significant difference as compared to M group at the corresponding reperfusion time points in each group or sham group. In contrast, the mRNA expression of GLUT3 in IPC group increased at 24 h, and was the highest at 48 h as compared to cerebral ischemia group at the corresponding reperfusion time points or sham group.CONCLUSION: 3-NPA preconditioning increases the expression of GLUT1 and GLUT3 at protein and mRNA levels to maintain the energy supply in brain tissues, indicating a cerebral protective mechanism.

Management of patient history(PH) is of great significance in medical information management.For doctors,to achieve the best curative effect,thoroughly comprehending PH and,on the base of which,fashioning effective treatment plans and suggestions and conducting systemic and integrated treatment program,is prerequisite.For medical research,integrate PH document is the foundation of further clinical research.Aiming at the inevitable difficulty for medical consultation and medical research resulting from traditional paper-document-record of PH,discussed in detail SQL server access and C/S system structure correlating to the installation of PH database management system as well as the layout and implementation of PH inquiring system.Procedure of the design and implementation of PH inquiring system.Through discussion,certain PH database management system can completely satisfy the medical establishment's request for PH management.

Discuss that designing the database management,how to realize authorization management by friend class programming in database security management.Utilizing friend function method in C++,that is,friend function basic idea and design method and friend class use rule,profit authorization management.Dependent on appropriate program analysis,gives right friend function design method and basic rule.Using the friend function design,completely achieve database management,but must give up encapsulation character of class.

Although our knowledge of ADRF(adipocyte-derived relaxing factor) is extremely limited,the little that is known has already revealed a promising future in this newly found factor.Firstly,it is secreted by adipose tissue,which is a abundantly and extensively distributed in human body and has become a hot spot for research work in recent years.Secondly,ADRF has shown a significant vasodilative action in a considerable number of experiments conducted on arteries of various sizes,from different body parts of different species of animals,including human.In this article,we introduce the development of ADRF research,sum up its known properties,including its Ca2+,protein tyrosine kinase,and protein kinase A dependent releasing,K+ channel mediated functioning,and interfered effect in different pathological models,and propose problems surrounding this factor and directions for future research work.

Discuss application about class of event created by control for solving that many specialty men have meet puzzles in visual graphic form program design.Utilizing visual basic program design,indicate basic concept and methods of event class application and list appropriate programs.Dependent on analysis above,gives right method and rule about event class design.The design of class of event created by control must be considered its application transparence and modification seal.

AIM: To explore the effect of high purified human chorionic gonadotropin (hCGs) and glycodelins isolated from amniotic fluid and serum of pregnant women on E-selectin mediated endothelial cell adhesion. METHODS: The hCGs and glycodelins in human amniotic fluid and serum collected from pregnant women were purified by using chromatography. The binding of ~ 51 Cr-labelled HepG2 cells to IL-1β stimutated human umbilical vein endothelial cells (HUVECs) in the presence of increasing concentrations of hCGs and glycodelins was quantified. The effect of hCG and glycodelin on E-selectin-mediated endothelial cell adhesion was observed. RESULTS: HCGs isolated from amniotic fluid and serum of pregnant women inhibited the E-selectin mediated adhesion of HepG2 cells to HUVECs in a dose-dependent manner, the IC_ 50 was 3.2×10~ -6 mol/L and 6.3×10~ -6 mol/L, respectively and the relative inhibition was 1.5×10~4 and 1.1×10~4, respectively, compared to sialyl-Lewis~x. The IC_ 50 of amniotic fluid glycodelin and serum glycodelin was 3.2×10~ -6 mol/L and 6.3×10~ -6 mol/L respectively, and the relative inhibition was 7.2×10~2 and 3.7×10~2, respectively, compared with sialyl-Lewis~x. CONCLUSION: Pregnancy-associated glycoproteins, hCG and glycodelin, are the powerful inhibitors for E-selectin-mediated endothelial cell adhesion.

AIM: To investigate the influence of hepatic portal interdicting on gastric mucosa of the liver cirrhosis rats and the protective effect of famotidine on gastric mucosa. METHODS: Fifty Wistar rats were randomly divided into 5 groups; group A (healthy rats), group B (healthy rats with interdicted hepatic portal), group C (liver cirrhosis rats without interdicted hepatic portal), group D (liver cirrhosis rats with interdicted hepatic portal) and group E (liver cirrhosis rats without interdicted hepatic portal and use of famotidine); The gastricfluid pH, gastric mucous content (GMC), gastric mucosa blood flow (GMBF) and the damage index of gastric mucosa (DIGM) of the every group were observed. RESULTS: There is no significant difference in the above parameters between group A and group B (P0.05). Compared with group A, GMBF of group C, D, and E were reduced (P0.01), pH, GMC of group C, D and E were also reduced (P0.05); DIGM of group C, D and E were increased (P0.01). All the changes of group D were more obvious than those of group C (P0.01); While there was no significant difference in all the parameters between group E and group C (P0.05). CONCLUSION: Hepatic portal interdicting can aggravate the gastric mucosa damage of the liver cirrhosis rat, and famotidine can protect against such gastric mucosa injury through improving the microcirculation of gastric mucosa.

目的探讨来源于孕妇羊水和血清的高纯度Glycodelin对E型选择素介导的细胞黏附过程的影响程度.方法以色谱仪分离和纯化孕妇羊水和血清的Glycodelin,测定铬51标记的肝癌细胞系-HepG2细胞在不同来源、不同浓度Glycodelin作用下和重组E型选择素的黏附能力,从而评价Glycodelin对E型选择素介导的细胞黏附过程的影响.结果血清和羊水Glycodelin能有效抑制Hepg2细胞和重组E型选择素之间的黏附,其抑制作用呈浓度依赖关系,其相对抑制力分别为纯四糖结构Sialyl-Lewisx(sLex)的9.4×105和4.0×105倍.结论妊娠相关糖蛋白Glycodelin高度抑制E型选择素介导的细胞黏附.

Discuss application about control property class for solving that many specialty men have meet puzzles in visual graphic form program design.Utilizing visual basic program design,indicate basic concept and methods of property class application and list appropriate programs.Dependent on analysis above,author gives right method and rule about property class design.The example shows that the design of control property class must be considered its application transparence and modification seal.

AIM: To study the expression and significance of osteopontin (OPN) in endometrial carcinoma and cervical cancer. METHODS: Immunohistochemical S - P assay was used to detect the expression of OPN in paraffin - embedded sections of 30 cases of endometrial carcinoma, 20 cases of cervical cancer and 30 cases of normal control tissues. The relationship between OPN expression and clinical- pathological characteristics was evaluated. RESULTS: The positive immunostaining rates of OPN in endometrial carcinoma (70% ) and cervical cancers (55% ) were significantly higher than that in the normal secretive and proliforative endometrium (50% and 10%) and normal cervical epithelium (10%), respectively (P<0.01). The positive immunostaining rate of OPN in sopiamous cell carcinoma and adenocarcinoma of the cervix was 53.3% and 60% , respectively, there was no significant difference between the two groups (P>0.05). The positive immunostaining rate of OPN in stage Ⅲ and stage Ⅱand G3 and G2 of endometrial carcinoma was significantly higher than that in stage Ⅰ and type G1 of endometrial carcinoma. The positive immunostaining rate of OPN in stage Ⅱ b and type G3 of cervical cancer was significantly higher than that in stage Ⅰa and type G1 of cervical cancer. CONCLUSION: OPN is significantly highly expressed in both endometrial carcinoma and cervical cancer, and its expression is closely related to the stage and grading of these malignant tumors.

AIM: To investigate the relationship among MCP-1 and monocyte chemoattract protein activity (MCA) and pathogenesis of lung cancer. METHODS: 173 patients were involved in the study and divided into three groups: group A: lung cancer group (60 patients); group B: benign lung disease group (55 patients) and group C: healthy control group (58 patients). MCP-1 level and MCA in bronchoalveolar lavage fluid (BALF) were measured. RESULTS: The concentration of MCP-1 and MCA in BALF in group A were much higher than those in group B and group C (P0.01), but the increase in MCA were not in direct proportion to the increase in MCP-1 in the patients with lung cancer. CONCLUSION: Monocyte chemoattract activity of the patients with lung cancer may be insufficient.

Objective: To explore the effect of HCGs isolated from human amniotic fluid, serum, and urine of pregnant women on cell adhesion mediated by E-selectin. Methods: The adhesion properties of 51 Cr-labelled HepG2 cells, which carry the ligands of membrane-bound sialyl-Lewis x epitope for E-selectin, were detected to recombinant E-selectin by means of adding different concentrations and sources of HCGs as potential inhibitors, and meantime, the effect of HCGs isolated from amniotic fluid, serum and urine in E-selectin mediated cell adhesion was assessed. Results: HCGs isolated from human amniotic fluid and serum of pregnant women inhibited the adhesion of HepG2 cells to E-Selectin in a dose-dependent manner, the relative inhibition was 2.4×10 4 and 4.5×10 3, respectively, compared with sialyl-Lewis x. But HCG isolated from urine of pregnant women did no effect. Conclusions: HCGs isolated from human amniotic fluid and serum of pregnant women is an effective ligand for E-selectin.

AIM: To investigate the effect of hypoxia on glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-actin and endothelin-converting enzyme (ECE)-2 mRNA levels in cultured mouse brain astrocytes (AC). METHODS: AC from neonatal mouse brain was incubated for 24 h in serum-free medium under hypoxic or normoxic conditions. The amount of transferred RNA was estimated using ethidium bromide stained 28S rRNA and 18S rRNA. The levels of tested mRNA were evaluated by Northern blot RNA hybridization. RESULTS: The GAPDH mRNA was up-regulated to 503.0% of the normoxic controls in hypoxic AC (n=10. P0.01), while the level of β-actin mRNA in hypoxic AC was down-regulated to 50.3% of control levels (n=6. P0.01). In contrast, the ECE-2 mRNA level was not affected by hypoxia. CONCLUSION: The results indicate that GAPDH and β-actin are not suitable for the internal standards for quantitative mRNA expression analysis in AC after hypoxia or ischemia. ECE-2 may function as an alternative in such circumstance.

AIM:To observe protective effect of L-glutamine on myocardial cell injury induced by endotoxin. METHODS:22 male SD rats,weighting(250±30)g,were randomly divided into three groups:control group( n= 7),endotoxin group( n= 7),glutamine/endotoxin group( n= 8). Heart were isolated from rats and perfused on Langendorff apparatus with Krebs- Henseleit(K-H)buffer(Saturation 95%O 2+5%CO 2)at a constant pressure(8.33 kPa)and temperature(37℃). The activity of superoxide dismutase (SOD)and malondialdehyde (MDA)content of efflux from coronary artery, monophasic action potential(MAP)and contractile force were measured at certain timepoints (0 min,20 min,50 min,80 min). RESULTS: Monophasic action potential and contractile force were markedly improved in Gln/ET group. The activity of SOD and MDA level in Gln/ET group restored closely to that in control group. CONCLUSION:L-gluta mine protects myocardial cells from injure induced by endotoxin.

本文论述了综合性大学医学院办学的优势;并对如何充分利用有利条件,真正办出综合性大学医学院的特色进行了探讨.

AIM: To further investigate the role of centralcorticotropin-releasing hormone in stress-induced hyperthermia and lipopolysaccharide (LPS)-induced fever in the rat. METHODS: Test substances were administered intracerebroventricularly (icv) via a third ventricle cannula. Body temperature responses were monitored at 30 min intervals using colonic thermistor probes. Arginine vasopressin (AVP) level in the ventral septal area (VSA) determined by radioimmunoassay. RESULTS: In normal saline controls, rats were handled to take the colonic temperature, their body temperature significantly increased with a peak of （0.88±0.31）℃. The injection (icv) of α-helical CRH(9-41), a CRH-41 receptor antagonists, markedly attenuated the stress-induced hyperthermia within 90 min after injection of normal saline. LPS(300 ng, icv) stimulated a biphasic rise in the colonic temperature, the 3.5 h thermal response index (TRI3.5) and AVP levels in the VSA of LPS-treated rats were higher than those of control rats. The AVP responses to LPS were inhibited significantly by blockade of central CRH actions using α-helical CRH(9-41) (5 μg ,icv) administered 10 min prior to LPS, while α-helical CRH(9-41) (5 μg ,icv) resulted in exacerbated febrile responses to LPS(300 ng, icv). CONCLUSION: Central CRH plays an important role in stress-induced hyperthermia. The injection (icv) of α-helical CRH(9-41) enhances markedly LPS-induced fever in rats. CRH is a dual action molecule in LPS-induced fever, which itself mediates LPS-induced fever, at the same time, and limits the rise in body temperature during fever through actions of AVP in the VSA and glucocoticoids.

目的:近年来的研究表明,促肾上腺皮质激素释放激素(corticotropin releasing hormone, CRH)是一种重要的中枢发热介质.有资料表明,CRH在下丘脑发挥生理作用涉及cAMP依赖性和非cAMP依赖性两种不同的机制,但CRH发挥致热作用的中枢机制尚不明了.本研究观察了cAMP依赖性蛋白激酶信号传导通路在大鼠CRH性发热机制中作用.结果:第三脑室微量注射CRH(2.5 μg, 5.0 μg, 10 μg)引起大鼠结肠温度和下丘脑cAMP水平明显升高,下丘脑cAMP水平与大鼠2 h发热反应指数之间存在明显的正相关(r=0.994, P＜0.01).事先向第三脑室微量注射腺苷酸环化酶抑制剂2',3'-dideoxyadenosine(DDA, 30 μg)或cAMP依赖性蛋白激酶抑制剂Rp-cAMPs(15 μg)均显著抑制中枢注射CRH引起的发热反应.结论:CRH性发热的中枢机制与CRH激活cAMP依赖性蛋白激酶信号传导通路有关.

目的和方法:促肾上腺皮质激素释放激素(corticotropin releasing hormone, CRH)是一种重要的中枢发热介质.为进一步观察CRH在大鼠LPS性发热中的作用,本研究观察了第三脑室注射CRH受体拮抗剂α-helical CRH(9-41)对SD大鼠LPS性发热及脑中隔区精氨酸加压素(arginine vasopressin, AVP)含量的影响.结果:第三脑室注射300 ng的LPS引起大鼠结肠温度双相性升高,其3.5 h发热反应指数(thermal response index, TRI3.5)明显高于生理盐水对照组.事先向第三脑室注射α-helical CRH(9-41)(5 μg)再注射LPS组,TRI3.5明显高于单独注射LPS组,而脑中隔区AVP含量明显低于脑室单独注射LPS组,脑室单独注射α-helical CRH(9-41)组和对照组比较,脑中隔区AVP含量和TRI3.5没有明显差别.结论:脑室注射α-helical CRH(9-41)明显增强大鼠LPS性发热,CRH可能通过诱导脑中隔区AVP等发热体温负调节介质的生成发挥限制大鼠LPS性发热的作用,但并不能排除CRH的致热作用.