目的 探讨不同剂量维生素D(本实验采用VD3)对溃疡性结肠炎(ulcerative colitis,UC)小鼠结肠β-防御素-2的表达及意义.方法 建立C57BL/6小鼠UC模型,并分别给予不同浓度VD3干预,疾病活动指数(DAI)、组织学评分、免疫组化检测小鼠结肠黏膜中β-防御素-2表达.结果 高、低剂量VD3干预组DAI、组织学炎症、β-防御素-2表达明显低于UC组(P＜0.05),高、低剂量VD3组比较差异有统计学意义(P＜0.05),小鼠组间炎症同β-防御素-2表达具有相关性(P＜0.05).结论 维生素D对DSS诱导的急性UC小鼠具有一定的缓解作用,并存在剂量效应关系.

Purpose: The rapidly rising incidence of esophageal adenocarcinoma (EAC), which is usually diagnosed late with a poor prognosis, has become a growing problem. This study investigated the potential transcription factor (TF)-related molecular mechanisms of EAC by using bioinformatics analysis and qRT-PCR validation. Methods: Expression profile datasets for mRNAs (GSE92396, GSE13898, GSE26886 and GSE1420) and miRNAs (GSE16456) were downloaded from the GEO database. Overlapping differentially expressed genes (DEGs) and differentially expressed miRNAs (DEMs) were identified through integrative analysis. Then, a TF-miRNA-mRNA network was constructed based on bioinformatics data from the TRRUST, TRED and miRTarBase database. Furthermore, overall survival analysis for the mRNAs and miRNAs in the TF-miRNA-mRNA network was performed with data from TCGA, and qRT-PCR was used to validate the results. Results: A total of 294 overlapping DEGs were identified in EAC tissues compared to normal tissues, including 181 downregulated and 113 upregulated genes. Then, 16 TFs that could target the DEGs and were related to cancer were predicted based on public databases, and 41 DEGs that could be targeted were identified as key genes. Additionally, 12 DEMs were predicted through miRTarBase to be associated with the key genes, and TP53-(miR-125b)-ID2 and JUN-(miR-30a)-IL1A from the TF-miRNA-mRNA network were identified to potentially play significant roles in EAC. Furthermore, CCL20, IL1A, ABCC3, hsa-miR-23b, and hsa-miR-191, which are involved in the TF-miRNA-mRNA network, were found to be significantly associated with patient survival in EAC. Finally, the expression of a miRNA-mRNA pair (hsa-miR-30a-5p and IL1A) was revealed to be correlated with prognosis. Conclusion: In this study, a TF-miRNA-mRNA network was constructed to analyze the potential molecular mechanisms of EAC. Key genes and miRNAs associated with patient survival were identified, which may reveal promising approaches for EAC diagnosis and therapy.

目的 探究抗焦虑抑郁药治疗功能性腹痛综合征(FAPS)的临床疗效,验证焦虑抑郁精神心理因素与FAPS的相关性.方法 将67例FAPS患者随机分为试验组(34例)和对照组(33例).两组患者均给予抑酸、促动力等个性化常规治疗,试验组在常规治疗基础上加用盐酸帕罗西汀(赛乐特),随访4周.在治疗0、4周时,分别对两组患者腹痛视觉模拟评分(VAS)、PHQ-9抑郁症筛查量表评分、GAD-7焦虑症筛查量表评分进行比较.结果 治疗后,两组患者的腹痛VAS评分、PHQ-9评分、GAD-7评分均较治疗前下降,且试验组低于对照组(P<0.05).结论 盐酸帕罗西汀对改善伴有焦虑抑郁情绪障碍的FAPS患者的腹痛症状及情绪障碍具有显著疗效.