Effect of compositions and physical properties on 3D printability of gels from selected commercial edible insects: Role of protein and chitin

Compositions and rheological properties of alternative protein sources, including honey bee pupa, grasshopper, cricket, earthworm, and scorpion, and their relationships with 3D printing behaviors were investigated. Protein was found to be the major composition in all insects, while chitin exhibited the most variation. At optimal moisture contents, honey bee pupa and earthworm gels displayed sufficient fluidity but resulted in unstable printed structures, as observed visually and microstructurally. Grasshopper and scorpion gels possessed weak fluidity but produced more stable printed structures. Cricket gel exhibited the most balanced flow behavior and self-supportability. Protein-to-chitin mass ratio proved to be a main factor affecting the 3D printing behavior of the gels. Possible mechanisms on how compositions and properties affected the printing behavior of the gels were proposed. Suggestions for improving the 3D printability of insect and invertebrate resembling insect gels were provided based on these proposed mechanisms.