The contribution of stress reactions to the genesis of endometrial hyperplasia (EH), especially in terms of the relationship between chronic stress and hypoxic damage in this pathology, is relevant and requires a detailed study. The purpose — to investigate the features of hypoxic damage to the uterine mucosa under chronic stress in patients with endometrial hyperplasia without atypia. Material and methods. We conducted a clinical, prospective, non-randomized study of 30 patients with endometrial hyperplasia without atypia (EHWA). TOBOL questionnaire was used to subjectively assess anxiety and stress levels in patients. To objectively analyze stress levels, salivary cortisol was measured using a competitive enzyme-linked immunosorbent assay (ELISA). The morphological study included histological and immunohistochemical (IHC) analysis of endometrial biopsies using ready-to-use mouse polyclonal antibodies to HIF-1-alpha. The results were processed using nonparametric statistical analysis. Results. Patients with EHWA exhibit ergopathic and sensitive attitudes toward illness. Elevated salivary cortisol levels were noted in every second case. IGCh-analysis of HIF-1-alpha expression in endometrial biopsies revealed that 21.5% [18.6, 25.4%] of cells were immunopositive. We also analyzed HIF-1-alpha expression levels in EHWA, depending on salivary cortisol levels, as well as in patients with a predominantly sensitive attitude toward the disease. Conclusion. The relationship between chronic stress and hypoxic damage to the uterine mucosa in EHWA demonstrates that chronic stress correlates with increased HIF-1-alpha expression in endometrial biopsies. This requires further research for practical application within the individualized approach to the treatment of patients with this nosology.
Aim. To assess the associations of regional living conditions with the probability of hypertension (HTN), disease awareness, pharmacotherapy, and blood pressure monitoring in the elderly population, taking into account comorbidity. Material and methods. The analysis utilized individual data obtained from the ESSE-RF3 cross-sectional epidemiological study, conducted between 2020 and 2022. Regional living conditions were assessed using socio-geographic, demographic, economic, and production and environmental indices. The "Comorbidity of Patients with Noncommunicable Diseases in General Practice: Eurasian Guidelines" document was used to assess comorbid conditions. Logistic regression was used to evaluate associations, calculating odds ratios (OR) and 95% confidence intervals (CI). Results. In the sample with comorbid conditions, the HTN probability is associated with an increase in the Demographic Index (OR 1,37; 95% CI: 1,21-1,57). An increase in the Economic Index is associated with a decrease in HTN awareness (OR 0,68; 95% CI: 0,54-0,86). A decrease in pharmacotherapy is associated with an increase in the Production and Environmental Index (OR 0,91; 95% CI: 0,82-1,00). Improvement in blood pressure monitoring is observed with an increase in the SocioGeographical Index (OR 1,21; 95% CI: 1,11-1,32). In a sample without comorbidity, the HTN probability is associated with an increase in the Socio-Geographical Index (OR 1,24; 95% CI: 1,16-1,33), Demographic Index (OR 1,12; 95% CI: 1,04-1,21), and Production and Environmental Index (OR 1,08; 95% CI: 1,02-1,14). Higher awareness is observed with an increase in the Socio-Geographical Index (OR 1,29; 95% CI: 1,121,49). Improved blood pressure monitoring is noted with an increase in the Socio-Geographical Index (OR 1,17; 95% CI: 1,02-1,33). Conclusion. Regional living conditions are associated with the probability, awareness, pharmacotherapy, and monitoring of HTN in the elderly population.
Цель работы – создание способа экспериментального моделирования воспаления в околоносовых пазухах при хронической интоксикации табачным дымом в эксперименте у животных с длительным стажем табакокурения. Способ заключается в том, что крыса породы Вистар помещается на 1,5 ч ежедневно в течение 6 месяцев в ингаляционную камеру, вдыхая табачный дым сигарет с содержанием никотина – 0,8 мг, смолы – 11 мг, с концентрацией угарного газа в табачном дыме 98 мг/м³. Контрольную группу табачным дымом не ингалируют. Через 6 месяцев проводится эвтаназия животных и последующий анализ аутопсийного материала, подтверждающий развитие воспаления в околоносовых пазухах крыс основной и контрольной групп. Статистическую обработку данных проводили в программе Microsoft Excel 2010 с определением средней арифметической, среднего квадратичного отклонения, t-критерия. Различия считали достоверными при р ≤ 0,05. Технический результат заключается в более коротком сроке – 6 месяцев (по сравнению с другим способом – 8,4 месяца), с меньшим временем нахождения животного в камере – 1,5 ч (а не 2 ч). В эксперименте использовались сигареты с меньшим содержанием смолы – 11 мг вместо 25 мг, никотина – 0,8 мг взамен 1,4 мг, угарного газа – 98 мг/м³ (при другом способе – 265,5 мг/м³). Заявляемый способ позволяет в более короткие сроки, снизив расходы на табачные изделия при моделировании воспаления в околоносовых пазухах у крыс, на содержание животных, с меньшим токсическим воздействием табачного дыма в эксперименте, изучать вопросы патогенеза, контроля воспаления, использования методов коррекции, уменьшения стоимости обеспечения самой модели. The aim of the work is to create a method for experimental modeling of inflammation in the paranasal sinuses in animals with chronic intoxication with tobacco smoke. The method is characterized by the fact that a Wistar rat is placed for 1.5 hours daily, for 6 months, in an inhalation chamber, inhaling tobacco smoke of cigarettes with a nicotine content of 0.8 mg, a tar content of 11 mg, with a carbon monoxide concentration in tobacco smoke of 98 mg/m³. The control group is not exposed to tobacco smoke. After 6 months, the animals are euthanized and the autopsy material is analyzed to confirm the development of inflammation in the paranasal sinuses of the rats in the main and control groups. Statistical analysis was performed using Microsoft Excel 2010, including the calculation of the arithmetic mean, standard deviation, and t-test. The differences were considered significant at p ≤ 0.05. The technical result is a shorter period of 6 months (compared to the other method of 8.4 months), with a shorter time of 1.5 hours (instead of 2 hours) for the animal to be in the chamber. The experiment used cigarettes with a lower tar content of 11 mg instead of 25 mg, nicotine content of 0.8 mg instead of 1.4 mg, and carbon monoxide content of 98 mg/m³ (instead of 265.5 mg/m³). The claimed method allows for a shorter time period, reducing the cost of tobacco products when simulating inflammation in the paranasal sinuses in rats, and reducing the toxic effects of tobacco smoke in the experiment, allowing for the study of pathogenesis, inflammation control, and the use of correction methods, while also reducing the cost of maintaining the model itself.
This paper analyzes the author’s own experience in conducting the initial accreditation (IA) procedure for specialists. The study identified notable factors, specifically weaknesses in the training of graduates, which serve as a basis for addressing these issues.
The mixture of ozone and oxygen (O3–O2, 5:95) is called "medical ozone." Ozone therapy, which uses an ozone-oxygen mixture, is applied for a wide range of diseases, including certain cervical diseases. It is also used in other branches of medicine, such as the treatment of purulent wounds, inflammatory-degenerative diseases, and circulatory disorders. In cervical diseases, low concentrations of ozone used therapeutically do not cause toxic effects and exhibit immunomodulatory, anti-inflammatory, bactericidal, antiviral, fungicidal, antihypoxic, reparative, and related effects. Ozone therapy has shown efficacy in the treatment of benign cervical diseases, including those associated with HPV, which justifies its further research. For severe dysplasia, ozone therapy may be considered an adjunctive method of rehabilitation, rather than a substitute for surgical treatment.
