[目的]番茄红素合成后会在β-番茄红素环化酶SlLCY-B(lycopene-β-cyclase)催化下进入代谢途径,抑制SlLCY-B2 的表达可增加番茄红素的积累.对番茄红素代谢调控基因SlLCY-B1 的分子特征及sgRNA进行系统分析,为利用CRISPR/Cas9 技术对SlLCY-B2 及其上游启动子序列进行定点突变或片段删除、调控SlLCY-B2 的表达以减少代谢的途径,提高番茄果实的番茄红素含量.[方法]利用生物在线工具,对SlLCY-B2 多肽特性及保守结构域、基因组结构、数字表达谱、启动子顺式作用元件进行系统分析,根据CRISPR-Cas9 靶点设计原则,设计筛选出适宜的sgRNA.[结果]SlLCY-B2 位于番茄 6 号染色体,编码 498个氨基酸,gDNA不含内含子,呈果实特异性表达.SlLCY-B2 分布 145 条 sgRNA,其中 21 条有高特异性.SlLCY-B2 上游-1 500 bp 启动子序列分布15 个顺式作用元件和112 条sgRNA.[结论]对SlLCY-B2 和其启动子区进行基因编辑,可调控SlLCY-B2 的表达,将提高番茄红素含量.
[Objective]In order to determine the suitable planting density of JI JC4 and JI JC7 in Hebei cotton area,the effects of different planting densities on growth and development,yield,quality and disease resistance were studied.[Methods]4 different planting densities(4.5 × 104,9 ×104,13.5 ×104 and 18 × 104 plant/hm2)were set to investigate the agronomic traits,yield,quality and disease resistance of cotton,and the optimum planting densities of JI JC4 and JI JC7 were comprehensively analyzed.[Results]With the increase of planting density,the plant height of JI JC4 and JI JC7 decreased,the height of the first node of fruit branch increased,and the whole growth period delayed.The lint yield of JI JC4 was higher with the in-crease of planting density,and the difference was obvious.The density of JI JC7 line had little effect on lint yield.Different planting densities had effect on micronaire value of JI JC4,on elongation of JI JC7,and had no significant effect on other cotton fiber quality indexes.With the increase of planting density,Verticillium wilt disease index decreased and its resistance increased.[Conclusion]Overall,the suitable planting density of JI JC4 and JI JC7 were 13.5 × 104 plant/hm2.
[Objective]Reproductive output and germination characteristicsof Salsola rosacea in the hope of providing relevant data support for future research on seed heteromorphism and providing theoretical basis for the study on the ecological adaptation mechanism and evolution of seed heteromorphism in desert conditions.[Methods]To study the seed polymorphism,Seeds were classified according to their shape,size,color and growth mode,and germination characteristics of different types of seeds were investigated.The plants are gra-ded according to the dry weight of the plant,and then the reproduction distribution and seed output of plant are calculated.[Results]Three types of seeds were produced in S.rosacea.Type A was green and winged with high germination rate,and type B and C were yellow short-winged/wingless and could be dormant.The pro-portion of reproductive allocation of type A and B increased with increasing of plant class,which showed de-pendence on plant size,while type C showed no dependence.The output proportion of type A decreased with increasing of plant rank,while that of type B seeds increased with the increase of equal size which was de-pendent on the size of plant.No significant change was found in the output of type C seeds.[Conclusion]Three kinds of seeds with different dispersal patterns and germination behaviors can be produced in S.rosacea,and their reproduction allocation and seed output depend on plant size.
[Objective]To study the drought resistance at seed germination stage of six Liaomian cotton varieties under PEG simulated drought stress.[Methods]The seeds of six Liaomian varieties were used as the testing materials to study drought resistance at seed germination stage under different concentrations of PEG-6000(0,2.5％,5.0％,7.5％and 10.0％).Germination energy,germination rate,germination index,germination drought resistance index,hypocotyl length,root length,seedling fresh weight and root fresh weight were measured under different drought stress.[Results]The germination of cotton seeds was inhibited to dif-ferent degrees,and the germination energy,germination rate and germination drought resistance index were decreased with the increase of PEG concentrations.Seedling growth index and root growth index were signifi-cantly inhibited under 2.5％PEG stress.5.0％PEG stress promoted root length growth of all materials.The inhibition degrees of each index under PEG stress above 5.0％concentration were gradually increased with the increase of stress.[Conclusion]The six Liaomian cotton varieties were sorted according to their drought resist-ance as follows:Liaomian 10＞Liaomian 23＞Liaomian 45＞Liaomian 53＞Liaomian 49＞Liaomian 34.
[Objective]Its line for fresh and raisins,this project aims to select high yield and good quali-ty in the hope of providing the foundation for the grape's cultivation with high quality and high yield.[Meth-ods]18 diverse lines of this variety were taken as materials,Thompson seedless grape was used as control,the characters of bunch were described,the fruit quality was determined and drying test was carried out.[Re-sults]Bunch weight of long-bunch Thompson seedless and Ningxia Thompson seedless were 620.20 and 632.67 g respectively,which were 2.25 times and 2.29 times compared with CK.Berry weight of W3 was maximum and 3.10 g,followed by big-berry Thompson seedless and berry weight was 2.45 g,which was 2.35 times and 2.97 times respectively.The soluble solid content of 18 lines was lower than that of CK,the reducing sugar contents of big-berry Thompson seedless,W5 and W9 were 1.34％,1.91％and 3.17％higher than that of CK respectively,the sugar-acid ratio of W5,Zaowuhebai and Hongxiu were significantly higher than that of CK,and increased by 18.11％,23.34％and 22.97％,VC of big-berry Thompson seed-less and W3 were significantly higher than that of CK,and increased by 6.65％and 3.98.In terms of drying characteristics,dry rate of big-berry Thompson seedless and W8 were 6.65％and 3.98％higher than that of CK.In addition,shape index of long-berry Thompson seedless raisins was 3.10 and 1.46 times compared with CK,and popular in the market.Zaowuhebai was pink and raisins had unique appearance with yellow-red-reddish-brown color.[Conclusion]Long-bunch Thompson seedless,big-berry Thompson seedless and long-berry Thompson seedless have excellent performance,which,in addition,Zaowuhebai can be used as a characteristic strain.
[Objective]To analyze the effects of adding sodium selenite on the growth of fruiting body and agronomic traits of cultivated Morchella sextelata in the hope of providing theoretical and technical support for large-scale artificial cultivation of selenium-enrich Morchella.[Methods]The effects on different treatment of selenium concentrations on the yield and agronomic characters of Morchella sextelata were systematically ana-lyzed by artificial cultivation with Morchella sextelata as selenium-enrich carrier and sodium selenite as exoge-nous selenium.[Results]When the selenium concentration was 0,the length of cap and the circumference of stipe were the longest.When the concentration of selenium was 10 mg/L,the perimeter and length of the cap were the longest,which could promote the growth of Morchella sextelata.When the concentration of selenium was 20 mg/L,the yield,dry weight per plant and total length of fruiting body of Morchella sextelata were the largest,which promoted the growth of Morchella sextelata.When the selenium concentration was 40 mg/L,the selenium concentration inhibited the growth of Morchella sextelata fruiting body.The results showed that the comprehensive evaluation score of various indexes of Morchella sextelata fruiting body cultivated under the con-centration of selenium 20 mg/L was the highest,which was the best concentration.[Conclusion]Based onso-dium selenite as exogenous selenium,20 mg/L of selenium concentration is the best,which can be used as an ideal concentration for large-scale artificial cultivation of selenium-enrich Morchella.
[Objective]To study the effect of chemical detopping on long-fiber cotton in the hope of pro-viding theoretical support for integration matching techniques of chemical detopping.[Methods]The tested long staple cotton variety is Xin 78,A complete randomized experiment design was used to analyze the effects of seven detopping treatments:"Yiding","Yiding","Xianglingzhuan","Shiyifu","Miandingfeng","Guoguangguandingtaocan"and Manual topping on influences of cotton plant height,yield and yield compo-nents,main fiber quality.At the same time,fuzzy comprehensive evaluation method base on entropy weight was applied to evaluate the 11 characters of each treatment.[Results]The results showed that the plant height increment of pre and post the chemical detopping and artificial detopping treatments were 16.1-40.9 cm and 3.1 cm,respectively;"Yiding"treatent number of fruit branches pe cotton plant was compare with"Mianfeng-ding"and Manual topping separately high 1.5units,1.8untits.boll number per plant of"Yiding"and"Yi-ding"treatments was significantly higher than those of"Shiyifu","Miandingfeng"and artificial detopping treatments,the boll weight of the"Shiyifu"treatment was significantly higher than that of the"Guoguang-guandingtaocan"treatment by 0.23 g,lint percent of"Yiding"treatment was significantly higher than that of artificial detopping,"Yiding"and"Shiyifu"by 1.1,1.2 and 1.3percentage points,the seed cotton yield of"Yiding"treatment was significantly higher than that of"Shiyifu",artificial detopping and"Miandingfeng"treatment by 16.4％,17.4％and 20.8％.The lint cotton yield of"Yiding"treatment was significantly higher than those of"Guoguangguandingtaocan","Xianglingzhuan","Shiyifu",artificial detopping and"Mianding-feng"treatment by 15.4％,16.0％,20.8％,21.2％and 22.9％,the strength and the length uniformity in-dex had little difference,but it was significant for the micronaire value and length.The comprehensive evalua-tion of fuzzy method showed that the comprehensive performance of"Yiding","Yiding","Guoguangguand-ingtaocan"and"Xianglingzhuan"was better than artificial detopping.[Conclusion]The plant height incre-ment of chemical capping treatment is larger;chemical detpping has a significant effect on yield and yield com-ponents,micronaire value and length;"Yiding"treatment effect is the best,"Yiding","Guoguangguanding-taocan"and"Xianglingzhuan"treatment effect are second.
[Objective]In view of 4 type JMS residual film recycling machine in the face of complex to-pography,especially in the face of soft terrain,uneven terrain without copying function problem,this project aims to design a set of machine-electricity-fluid combined with automatic profiling system,that is the key part for further analysis and field experiment,for the ascension of the performance of the system in the hope of providing certain reference.[Methods]In this paper,the structure and working principle of 4JSM-2.1A re-sidual film recovery device were briefly introduced,and the main structure and working principle of the auto-matic copying system were described.The upper and lower copying movement of the key components of the copying ground wheel was deeply analyzed,and after that,it was found that the copying ground wheel would bounce when working.This phenomenon could be improved by increasing the spring stiffness coefficient and reducing the speed of the machine.Taking the stiffness coefficient of spring,the speed of machine and tools and the difference of ground fluctuation height as the influencing factors,and the qualified rate of soil depth as the experimental index,the orthogonal rotation test with three factors and three levels was carried out.[Re-sults]The field test results showed that the order of influencing test indexes was ground height difference,spring stiffness coefficient and machine speed,respectively.Finally,through optimization,the optimal param-eter combination was obtained as ground height difference of 20 mm,spring stiffness coefficient of 0.591 N/mm and machine speed of 6 km/h.[Conclusion]Under this parameter combination,the qualified rate of pen-etration depth can reach 93.47％,which is better than the standard value,indicating that the automatic profi-ling system has a good profiling effect.
[Objective]To study the effects of fenced enclosure and free grazing on growth patterns and endogenous hormone levels of Seriphidium transiliense,and to provide theoretical reference for vegetation resto-ration in degraded Artemisia desert grassland ecosystems.[Methods]Fencing and free grazing Seriphidium transiliense were taken as the research objects,and the methods in botany,plant physiology and turf manage-ment in the plant morphology,plant endogenous hormones and"four times the amount of"approach to Seriphi-dium transiliense plant height,coverage,density,biomass,primary branch number and secondary branch number were applied to conduct statistics,The dry weight of leaves,stem and root of each plant were meas-ured by drying method,and the contents of endogenous hormones(IAA,ABA,CTK,GAs and ETH)were measured by targeting method.Three transect lines were set for each treatment,and 40 quadrats were meas-ured by each transect line.Furthermore,the growth rule and endogenous hormone changes of Seriphidium transiliense under the two treatments were summarized.[Results]The plant height,biomass,leaf dry weight,stem dry weight and root dry weight were 2.57 times,29.73 times,7 times,7.87 times,5.21 times and 5.64 times,respectively of those fenced in Seriphidium transiliense.Fencing and free grazing had significant effects on CTK and ABA contents in leaves of Seriphidium transiliense(P＜0.05％).Except GAs,there was no significant difference in the content of endogenous hormones in stem of Seriphidium transiliense between the two treatments(P＜0.01％).Fencing had little effect on endogenous hormones of root system,but the con-tent of ETH was significantly different between them(P＜0.05％).[Conclusion]Fenced for 4 years,the morphological characteristics and growth trend of Seriphidium transiliense are in sharp contrast with the minia-tures and dwarfism of free grazing,especially the IAA and CTK contents of leaves and stems are more closely related to plant height and first-order branch number.
[Objective]Identification and analysis of TCP family genes in upland cotton can lay a founda-tion for studying the biological functions of TCP genes.[Methods]Based on the newly assembled upland cot-ton TM-1 reference genome in 2019,the TCP family HMM model file was obtained from Pfam website,the upland cotton TCP gene was identified using Hmmer website,and the upland cotton TCP genome protein se-quence was obtained from Cotton FGD website.MapInspect software was used for chromosome location,MEGA 7.0 was used for multiple sequence alignment cluster analysis,MEME website was used for motif prediction,TBtools software was used for gene structure identification and tissue-specific expression analysis of upland cotton.[Results]Sixty-three TCP genes of upland cotton were identified from the whole genome level,inclu-ding 39 subclasses of Class Ⅰ and 24 subclasses of Class Ⅱ.The Class Ⅱ subfamilies included 17 CIN subclas-ses and 7 CYC/TB1 subclasses.The 63 genes were distributed on 22 chromosomes of upland cotton,including 33 genes in group A and 30 genes in Group D.All TCP proteins contained TCP domains.The gene structure analysis results showed that the structures and lengths of exon and introns were similar in the same subfamily.12 of the TCP family genes were preferentially expressed in fibrous tissue,32 in flower organs,and 16 in roots,stems and leaves.[Conclusion]In this study,TCP genes related to flowering regulation and fiber de-velopment in upland cotton are identified and obtained by bioinformatics method.
[Objective]This project aims to evaluate the metabolic potential of strain TRM SA0054 by an-alyzing the genomic information,thus further studying the secondary metabolite gene resources of the strain.[Methods]The whole genome of TRM SA0054 was sequenced by Illumina Hiseq sequencing platform,and bioinformatics analysis was conducted.The metabolites were preliminarily detected and identified by modern separation and purification methods.[Results]The genome size of strain TRM SA0054 was 7.2 Mb,there were 6,410 protein coding genes,and GC content was 73.16％.AntiSMASH analysis predicted that there were 26 secondary metabolic gene clusters,among which Region 36.1 gene cluster was 81％similar to Tuber-cidin gene cluster.Fermentation products were detected by LC-MS,the fermentation of strain TRM SA0054 could produce Tubercidin.[Conclusion]Strain TRM SA0054 has the ability to produce Tubercidin based on genome mining and LC-MS detection.
[Objective]To study the effects of different phosphorus application methods on the growth of drip irrigation wheat and the utilization rate of phosphorus fertilizer in order to provide reference for the efficient utilization of phosphorus fertilizer in drip irrigation wheat.[Methods]Under the condition of field drip irriga-tion,winter wheat Xindong 41 was used as the experimental material.Under the condition of equal amount of nitrogen,phosphorus and potassium,six phosphorus fertilizer application methods were set:F0:no phosphorus application,F1:100％basic application,F2:75％basic application+25％topdressing,F3:50％basic ap-plication+50％topdressing,F4:25％basic application+75％topdressing and F5:100％topdressing to study the effects of different phosphorus application methods on dry matter,nutrient accumulation,yield and phosphorus fertilizer utilization rate of wheat.[Results]Phosphorus application significantly increased the dry matter and nutrient accumulation of wheat and increased the yield.Phosphorus application treatment increased the number of ears,grains per ear and 1,000-grain weight.The number of ears,grains per ear and 1,000-grain weight of phosphorus application treatment increased by 5.93％-23.99％,0.32％-8.76％and 1.78％-6.45％respectively compared with F0 treatment.There were differences in wheat yield and compo-nents under different phosphorus application treatments.The number of panicles,grains per panicle and 1,000 grain weight of F3 treatment were the best.The yield of F3 treatment was 10.21％higher than that of F5 treat-ment,and the difference was significant.The phosphorus fertilizer utilization rate,partial productivity and ag-ronomic efficiency of F3 treatment increased by 11.00％,4.81 kg/kg and 4.80 kg/kg respectively compared with F1 treatment.[Conclusion]F3 treatment(50％basal application+50％topdressing)has the best per-formance in population yield components and reaches high fertilizer utilization rate.
[Objective]In order to provide germplasm resources and theoretical basis for drought-resist-ant breeding,283 maize material were selected for comprehensive evaluation of drought-resistance coefficient and yield in well-watered under water-stressed.[Methods]Using the method of double selection of drought resistance coefficient and yield in well-watered,the changes of drought resistance,yield and phenotypic characteristics of maize in well-watered were analyzed and studied.[Results]The results showed that under well-watered and water-stressed,the yield was normally distributed,the drought resistance coefficient showed skewed distribution,the average drought resistance coefficient was 0.617,and the stress intensity was 0.383.15 excellent inbred lines and 3 crosses were selected for both high yield and drought resistance.The days of silking(58.5-71.0 days),plant height(150-208.1 cm),ear height(44.6-77.5 cm),number of grains per ear(295-502 grains per ear),and 100 grain weight(16.5-36.0 g)of inbred lines with ex-cellent drought resistance in well-watered and yield per plant(60.4-90.5 g)showed certain range charac-teristics.The hybrids with excellent drought resistance had days of silking(61.5-63.0 days),plant height(275.6-287.8 cm),ear height(103.8-115.0 cm),number of grains per ear(538-692 grains per ear),and 100 grain weight(34.9-38.9 g)in well-watered and yield per plant(165.7-186.8 g)showed a certain range.The yield per plant and drought resistance of excellent drought resistant materials were higher than the average level,while the growth period,plant height,spike height,grain number per spike and hun-dred grain weight were lower than the average level.Grain number per spike and hundred grain weight had a complementary relationship.[Conclusion]A total of 18 excellent maize materials with high yield and drought resistance are selected.The yield per plant of 15 inbred lines in well-watered is not less than 60 g,and the drought resistance coefficient was not less than 0.8.The yield per plant of the 3 hybrid combinations in well-watered is not less than 160 g,and the drought tolerance index of the varieties is greater than 1.5.The 18 ma-terials have high yield under normal irrigation conditions and strong drought resistance in terms of grain number per spike,which can be used in breeding.
[Objective]To screen out the most suitable planting density suitable for planting with 76 cm machine pick up cotton equal row spacing.[Methods]From April to October 2020,76 cm equal row spacing plastic film was used for planting,with 9 × 104 plants/hm2(A)and 12 × 104 plants field trials were conducted at five planting densities of/hm2(B),15 × 104 plants/hm2(C),18 × 104 plants/hm2(D),and 21 × 104 plants/hm2(E).Inclination angle(MTA),canopy opening(DIFN),light interception rate,canopy temper-ature,humidity,yield and other indicators were used to study the effects of different planting densities on cot-ton canopy structure,canopy temperature and humidity and yield.[Results]The LAI and leaf inclination an-gle of each treatment increased with the increase of planting density,which always showed E＞D＞C＞B＞A,and the canopy opening basically showed A＞B＞C＞D＞E;The light interception rate of each treatment ba-sically showed a"V"-shaped variation trend in space,and the higher the density,the lower the average tem-perature and the higher the average humidity in the canopy.When the treatment level was 15 × 104 plants/hm2,the number of bolls per plant and the weight of single bolls were significantly increased compared with the control,and the yield of seed cotton was 4,849.96 kg/hm2.The yields were increased by 41.94％,24.20％,4.72％and 15.20％respectively compared with other treatments.[Conclusion]Comprehensively considering,in this experiment,the density of 15 × 104 plants/hm2 can obtain a better cotton canopy struc-ture,which is beneficial to the canopy light distribution and obtains higher yield.
[Objective]To clarify the microbial diversity of sweet sorghum silage in the hope of providing a theoretical basis for silage production and storage.[Methods]In this study,the microbial diversity of silage from two varieties with or without the addition of silage additives were analyzed using high-throughput sequen-cing.[Results]The dominant bacterial phyla in sweet sorghum silage were Firmicutes,Proteobacteria,Bacte-roidetes,Cyanobacteria and Acidobacteria,and the dominant bacterial genera were Lactobacillus,Enterobact-er,Lactococcus and Corynebacterium.Ascomycota was the dominant phylum in fungal community.[Conclu-sion]The microbial flora composition of sweet sorghum silage is similar among different treatments,but the relative abundance is significantly different.Silage microbial agent reduces the relative abundance of microbial flora in sweet sorghum silage.
[Objective]To study the characteristics of volatile organic compounds(VOCs)in leaves of three walnut varieties,compare the differences of VOCs among leaves of each walnut,and construct the scent fingerprint of VOCs of three walnut varieties.[Methods]Gas chromatography-ion mobility spectrometry GC-IMS was used to analyze the odor fingerprints of volatile substances in the leaves of Hickory walnut,Wen185 and American red walnut,and variance analysis and principal component analysis were used to analyze the differences in the fingerprints of volatile substance odor in their leaves.[Results]The volatile substances were contained in the leaves of different walnut varieties including alcohols,esters,aldehydes,terpenes and a small amount of ketones and furans.The contents of ethyl acetate,β-pinene dimer and acetone in walnut were sig-nificantly different from those of Wen185.The contents of 2-vinylaldehyde,cineole and 2,5-dimethylfuran in walnut were higher than those of Wen185 and American red walnut.[Conclusion]There are significant differences in the fingerprint profiles of Hickory walnut,Wen185 and American red walnut,and the content of alcohol was higher in Hickory walnut,and the content of terpenes was higher in American red walnut.The content of Wen185 in esters was higher than that of Hickory walnut and American red walnut.
[Objective]To evaluate the control effect of pendimethalin EC,flumioxazin WP and their mixture to annual weeds and safety to cotton in the hope of providing scientific reference for chemical control to weeds in cotton field.[Methods]Plant control effect,fresh effect of 30 days and 40 days to annual weeds and safety to cotton after herbicides application were studied.[Results]The results showed that there were no difference of treatments on safety to cotton,and no significant difference on seedling emergence rate between each treatment and blank control.Plant control effects of 330 g/L pendimethalin EC(742.5 g/hm2)+50％flumioxazin WP(45 g/hm2)and 330 g/L pendimethalin EC(866.25 g/hm2)+50％flumioxazin WP(60 g/hm2)to annual weeds after 30 days were 96.52％and 97.91％,95.86％and 96.48％after 45 days,fresh weight control effects were 92.89％and 94.44％after 45 days,respectively.The mixture of pendimethalin and flumioxazin had significant effect on annual gramineous and broad-leaved weeds in cotton fields.The plant and fresh weight control effects of 330 g/L pendimethalin EC 866.25-990 g/hm2 to annual gramineous weeds were between 83.58％to 90.23％,and the effects of 50％flumioxazin WP 60-75 g/hm2 were be-tween 84.58％to 90.98％,no significant difference between them.The plant and fresh weight control effects of 330 g/L pendimethalin EC 866.25-990 g/hm2 to annual broad-leaved weeds were between 82.62％to 88.90％,and the effects of 50％flumioxazin WP 60-75 g/hm2 were between 85.06％to 92.21％.[Con-clusion]The plant and fresh weight control effect of flumioxazin to annual broad-leaf weeds are higher than that of pendimethalin,which can be applied selectively according to difference dominant weeds population for reasonable controling weeds in cotton field.
[Objective]This project aims to optimize the experimental conditions for the treatment of pota-to starch wastewater by coupling of Acidophilic photosynthetic bacteria(PSB)and Bacillus atrophaeus(BA).[Methods]Potato starch wastewater was used as study materials and they were divided into treatment and control groups.The treatment groups were the compounding ratio,inoculation,culture methods of the two strains and the wastewater contaminants degradation(like COD and Malodorous gas)rates were used to reflect their effects.Finally,we tested the total application effect of the optimized experimental conditions for the treatment of potato starch wastewater.[Results]The optimized experimental conditions for the treatment of potato starch wastewater were:the ratio of PSB to BA=10:1,15％of inoculation,30℃ of incubation,and incubated 144 h including light intensity 5,000 lx under anaerobic illumination for the first 72 h,then the next 72 h incubated under anaerobic illumination/aerobic=12 h:12 h.The COD degradation rate under the opti-mized condition was increased from 16.9％in control group(without inoculation)to 98.21％,and it also showed more than 98％of degradation rate of other contaminates(like ammonia nitrogen,nitrate nitrogen,to-tal phosphorus,mercaptan,hydrogen sulfide)and its self-flocculating rate was 8 cm/h.[Conclusion]The results suggested that the wastewater contaminated with high degradation rate by coupling of acidophilic photo-synthetic bacteria and Bacillus atrophaeus is mainly attributed to the niche differentiation of the optimal growth pH.
[Objective]To systematically understand the production and application value of new barley variety Mengpimai 5 in the hope of providing favorable data support for its wide application.[Methods]Based on the multi-point regional test and production test data of Mengpimai 5 for many years,the high and stable yield and adaptability of Mengpimai 5 were analyzed by comparative analysis,high stability coefficient method,genotype grouping method and adaptive parameter method,and the correlation between yield factors was clari-fied by combining with the path analysis of yield related traits.[Results]In the production demonstration,the average yield of Mengpimai 5 was 4,395.2 kg/hm2,which was 19.5％higher than that of the control,and the yield point rate was 87.5％.In the two-year regional test,the high stability coefficient was the largest,the coefficient of variation was the smallest,the adaptive parameters were less than 1,the 1000-grain weight was 53.8 g,the full grain was 93.45％,the germination rate was 98％,the malt extract was 80％,the Kurbaha val-ue was 40.5％,the saccharifying power was 435 WK,and the α-amino acid was 153 mg/100g.Among the yield components,grain number per plant(0.667)had the greatest direct impact on yield,followed by effec-tive panicle number(0.543)and 1,000-grain weight(-0.41).Grain number per plant and effective pani-cle number had a positive effect on yield formation of Mengpimai 5,while 1,000-grain weight had a negative effect on yield formation.[Conclusion]Mengpimai 5 has high and stable yield,strong adaptability,and its quality index meets the national standard of high-quality malting barley.It is suitable for planting in the mid-dle-eastern and western Inner Mongolia and spring wheat areas with similar ecological conditions.In order to ensure the maximum yield effect of Mengpimai 5 and promote its large-scale popularization and application,the basic seedlings should be increased as far as possible,but the cultivation density should be paid attention to prevent lodging.At the same time,timely fertilization and irrigation at tillering stage can promote effective tille-ring.
[Objective]To understand the effects of amine fresh and methylperidium compound on the growth and development of cotton,and to clarify the effect of amine fresh and meperamide compound on cotton yield increase of different foliar fertilizers,[Methods]The experiment was carried out in Dafeng Town,Hutu-bi County in 2021,using Xinluzao 82,mainly amine fresh and methyl,respectively,compounded acetyl-amine,auxiliary,borozin fertilizer,potassium scalemate,silicon fertilizer;Single administration of acetyl-amine was taken the control.In this experiment,the effects of different treatments on the agronomic traits,dry matter accumulation and yield quality of cotton were analyzed.[Results]The results showed that different treatments showed different degrees of regulation of plant height,among which the treatment of amine fresh me-peridium+additive+boron zinc fertilizer had a strong ability to inhibit plant height.In terms of dry matter accumulation,the amount of drifting dry matter accumulated by foliar sprays of amine fresh,meperamide+additive+potassium phosphite was the largest in all treatments,reaching 45.37 g/plant,and the total dry matter weight was the highest,reaching 83.71 g/plant;In terms of yield,the number of bolls per plant sprayed with amine fresh,meperidium+additive+boron zinc fertilizer reached a maximum of 6.78 per plant,and the maximum weight of a single bell was 6.41 g,and the yield of seed cotton harvested by spraying amine fresh o meperidium+additive+potassium phosphite was the highest,which was 10.42％higher than that of the control.[Conclusion]Therefore,the combination of amine fresh o meperidium+auxiliaries+potassium phosphite has the best yield enhancement effect.
