A method was developed for the determination of monofluoroacetic acid (MFA) in plasma and urine by ion chromatography-triple quadrupole mass spectrometry (IC-MS/MS). A plasma sample was extracted with 3% (v/v) perchloric acid aqueous solution, and the extract was centrifuged to remove the protein and lipids. A urine sample was acidulated with 3% (v/v) perchloric acid aqueous solution. The target analyte was extracted with methyl tert-butyl ether (MTBE) at a pH between 0.5 and 1.0. After the MTBE was removed by blowing with nitrogen, the MFA in the residues was dissolved into 0.1% (v/v) ammonia solution. The separation of MFA was carried out on a Dionex Ionpac AS 19 analytical column (250 mm×2 mm, 7.5 μm) with gradient elution using KOH solution electrolytically generated from an on-line eluent generation cartridge. Before the eluent flow entered the mass spectrometer, an in-line suppressor was used to remove potassium ions. The MFA was detected with a negative electrospray ionization source in the multiple reaction monitoring (MRM) mode, and quantified with the stable isotope internal standard method. The correlation coefficient of the linear calibration curve of MFA was greater than 0.999 at the corresponding ranges of 0.1-1000 μg/L. The average recoveries were 96.2%-120% of MFA in plasma and urine samples with relative standard deviations of 1.1%-13.1% (n=6). The limits of detection of MFA in plasma and urine samples were 0.03 μg/L and 0.1 μg/L, respectively. The method is simple, sensitive and accurate, and can be applied for the determination of MFA in plasma and urine samples.
医疗健康数据是一种敏感的个人信息载体,在数据挖掘、开放、交易和共享上需要法律指引.俄罗斯采用的法律技术路径是"修订卫生基本法律,配套个人信息保护法律,制定新兴实验法律".在人工智能和数字创新的实验性法律中,俄罗斯创设了匿名数据处理的主体及提案的审批路径.为使医疗健康数据合法合理利用,我国可通过修订《基本医疗卫生与健康促进法》增加医疗秘密部分,明确数据处理主体及项目的资格、条件和规则,制定匿名数据加工利用的实施细则,推动医疗健康大数据在科学研究、产业转化、公共事业中的积极应用.
目的 探讨双歧杆菌制剂对肿瘤化疗患者肠道菌群的影响.方法 通过检索PubMed、Embase、The Cochrane Library、Web of Science 以及 CBM、CNKI、WanFang Data 和 VIP 数据库,收集各数据库从建库至2022年7月发表的所有关于肿瘤化疗患者应用双歧杆菌制剂的随机对照试验,由2名研究者独立筛选文献,按照系统评价的要求对文献质量进行评估,使用Revman5.4和Stata17.0统计学软件进行Meta分析.结果 共纳入25篇文献(2 152例患者),Meta分析结果显示,双歧杆菌制剂组患者肠道中双歧杆菌和乳杆菌的数量高于对照组[SMD=2.89,95％CI(2.28,3.49),P＜0.001;SMD=2.20,95％CI(1.57,2.84),P＜0.001],双歧杆菌制剂组患者肠道中大肠埃希菌、肠杆菌和肠球菌的数量低于对照组[SMD=-1.08,95％CI(-1.64,-0.52),P＜0.001;SMD=-0.88,95％CI(-1.58,-0.17),P=0.010;SMD=-0.98,95％CI(-1.68,-0.28),P=0.006].结论 在化疗的基础上添加双歧杆菌制剂对肠道主要菌群水平能起到显著的改善作用,降低不良反应发生率,值得在临床上推广及应用.
目的 以结肠癌术后患者粪便中分离出的鼠李糖乳杆菌PUM1749作为研究对象,通过检测其耐高盐、耐胆盐能力和抑菌活性,评价其益生特性.方法 以鼠李糖乳杆菌GG为对照,将其和鼠李糖乳杆菌PUM1749分别培养于含不同浓度NaCl和胆盐的MRS肉汤中,采用滴种法进行活菌计数,采用双层琼脂夹心法结合滴种法检测两株菌对8种指示菌的抑菌效果.结果 在不同浓度NaCl溶液培养24h后,2株鼠李糖乳杆菌活菌数均在108CFU/mL数量级以上.当浓度低于2g/100mL时,2株菌活菌数随NaCl浓度增加而减少,之后随着NaCl浓度的提高,PUM1749株活菌数均高于GG株;在不同浓度胆盐肉汤中培养4h后,2株菌生长均被不同程度抑制,活菌数显著下降,PUM1749株在胆盐浓度为0.1g/100mL时生长被完全抑制,但继续提高胆盐浓度后,PUM1749株活菌数反而明显优于GG株;2株菌对8种指示菌均具有较好的抑菌效果,且对革兰阳性菌和革兰阴性菌的抑菌效果无明显差别.结论 鼠李糖乳杆菌PUM1749在不同高盐和胆盐浓度条件下的耐受能力和抑菌活性均强于或持平鼠李糖乳杆菌GG.鼠李糖乳杆菌PUM1749具有良好的益生特性.
目的 分析宫颈上皮内瘤变(CIN)患者阴道微生态与人乳头瘤病毒(HPV)感染的关系.方法 选取326例本院收治的CIN患者,采集阴道分泌物和宫颈脱落细胞,通过形态学和功能学检测阴道微生态状况,采用PCR-反向点杂交法进行HPV分型检测,采用二分类Logistic回归分析影响CIN患者HPV感染的因素.结果 CIN患者HPV阳性共256例,占78.5％,HPV阴性共70例,占21.5％.HPV阳性CIN患者白细胞计数＞10个/高倍视野、阴道清洁度Ⅲ-Ⅳ度、WC阳性、AV阳性、BV阳性、pH值＞4.5、白细胞酯酶阳性、过氧化氢阳性占比高于HPV阴性患者,微生态正常者占比低于HPV阴性患者(P＜0.05).二分类Logistic回归分析显示,WC、BV、微生态正常和pH值为CIN患者HPV感染的影响因素.结论 CIN患者HPV感染率较高,且感染后存在阴道微生态失调,VVC、BV、微生态异常和pH值是CIN患者HPV感染的影响因素.
