Objective:To observe the effects of Tongdu Xingnao acupuncture on cognitive function in rats with cerebral ischemia and reperfusion(CIR),and to explore the possible mechanisms.Methods:Among 65 male Sprague-Dawley rats,15 were randomly selected as the blank group,and another 15 were selected as the sham operation group.In this group of rats,only the blood vessels were subjected to a temporary separation operation,while the remaining 35 rats were used to construct the right middle cerebral artery ischemia-reperfusion injury model by the suture method.During the experiment,2 rats failed to model successfully and 3 rats died.Subsequently,the remaining rats were randomly divided into the electroacupuncture group and the model group,with 15 rats in each group.The effects of Tongdu Xingnao acupuncture on the cognitive function of CIR rats were evaluated through neurological deficit scores,Morris water maze tests,TTC staining,Nissl staining of neurons in the CA1 region of the hippocampus,HE staining of hippocampal tissue pathology,expression levels of SOD,MDA,and ROS in hippocampal tissue,serum levels of IL-1β,IL-6,and TNF-α,and the expression levels of Beclin-1 and LC3 proteins in the ischemic hippocampal tissue.Results:Compared to the blank group and sham operation group,the model group showed a significant improvement in neurological deficit scores(P＜0.01),a significantly prolonged escape latency(P＜0.01),a marked decrease in the number of crossings over the original platform(P＜0.01),and a significant increase in cerebral infarction volume(P＜0.01).The neurons in the CA1 region of the hippocampus in the model group exhibited incomplete morphology and a reduction in number,with fewer and disordered cell nuclei,an increase in glial cell nuclei,abnormal morphology and structure,a surge in inflammatory cells,and vacuolar structures.Serum levels of IL-1β,IL-6,and TNF-α were elevated in the model group(P＜0.05).In the rat hippocampal tissue,the levels of ROS and MDA increased,while SOD levels decreased in the model group(P＜0.05).The expression levels of Beclin-1 and LC3 proteins in the hippocampal region also increased in the model group(P＜0.05).Compared to the model group,the electroacupuncture group showed a significant decrease in neurological deficit scores(P＜0.01),a significantly shortened escape latency(P＜0.01),a significant increase in crossings over the original platform(P＜0.01),and a significant reduction in cerebral infarction volume(P＜0.01).The neurons in the CA1 region of the hippocampus showed improved morphology and an increase in number in the electroacupuncture group,with more organized cell nuclei and improved morphology and structure.Serum levels of IL-1β,IL-6,and TNF-α decreased in the electroacupuncture group(P＜0.05).The levels of ROS and MDA in the hippocampal tissue decreased,while the SOD levels increased in the electroacupuncture group(P＜0.05).The expression levels of Beclin-1 and LC3 proteins in the hippocampal region further increased in the electroacupuncture group(P＜0.05).Conclusion:Tongdu Xingnao acupuncture can increase the expression of Beclin-1 and LC3 in hippocampal tissue of CIR rats,improve inflammation and oxidative stress indicators,improve learning and memory ability,and reduce neuronal cell damage,which may be related to the regulation of autophagy network system.
As the national hub of acupuncture and moxibustion resources and international dissemination, Beijing is home to various acupuncture institutions that lead the development and globalization of acupuncture nationwide. Starting with a comprehensive overview of relevant literature on Beijing's international dissemination since the founding of the People's Republic of China, this paper, on the basis of reviewing and sorting out the historical context of Beijing's acupuncture international dissemination and related key studies, focuses on three aspects, including historical materials, experiences, and strategies. It analyzes the historical material clues and element clusters for advancing the research on Beijing's international dissemination history, and highlights the urgency of excavating oral historical materials. Based on the concise experience of the international communication of acupuncture and moxibustion in Beijing in terms of institutional platform construction, talent export, technological leadership, cultural integration, digital communication and other aspects, it identifies issues such as technology preceding culture, dilution of communication subjectivity and the impact of cultural colonialism in the historical process. Against the backdrop of the development of artificial intelligence (AI) and virtual reality (VR) technologies such as DeepSeek, Sora, VisionPro, etc., this paper prospects and initially explores strategies and academic directions including the construction of a "Chinese Acupuncture Culture" system, the simultaneous advancement of "hard content" and "soft forms" in acupuncture dissemination, the improvement of oral historical materials related to acupuncture dissemination, and the reconstruction of a symbiotic knowledge ecosystem of acupuncture culture.
Objective:To explore the mechanism of electroacupuncture(EA)in improving anxiety and depression like behavior in chronic sleep deprivation(CSD)mice based on metabolomics technology.Methods:Sixty Male Swiss mice were randomly divided into a control(CON)group,a model(MOD)group,an EA group and a fluoxetine(FLX)group,15 mice in each group.A CSD mouse model was established using a improving multi platform water environment method.The effect of EA on anxiety and depression like behavior was evaluated through tail suspension experiment(TST),forced swimming experiment(FST),open field experiment(OFT)and elevated cross maze experiment(EPM).After the behavioral experiment,serum samples were collected.The effects of EA on the serum levels of corticotropin releasing hormone(CRH),adrenocorticotropic hormone(ACTH)and corticosterone(CORT)were evaluated by enzyme linked immunosorbent assay(ELISA).Seahorse samples were collected and the effect of EA on endogenous metabolites in the hippocampus of mice was evaluated by non targeted metabolomics analysis.Results:Compared with the CON group,the MOD group showed an increase in TST and FST immobility time(P＜0.05 or P＜0.01),a decrease in the distance to center ratio,center time ratio,and the number of entries into center in the OFT(P＜0.05 or P＜0.01),and a decrease in the number of open arm entries and time spent in open arms in the EPM(P＜0.01).Compared with the MOD group,the immobility time of the TST and FST in the EA group and the FLX group decreased(P＜0.05 or P＜0.01),while the open arm entry ratio and open arm time ratio increased(P＜0.05).In the EA group,the central distance ratio and central time ratio increased(P＜0.05),and the number of entries into the center showed an increasing trend(P＞0.05).In the FLX group,the central distance ratio,number of entries into the center,and central time ratio decreased(P＜0.05 or P＜0.01).The ELISA showed that compared with the CON group,the MOD group showed increased levels of CRH,ACTH,and CORT(P＜0.01).Compared with the MOD group,the levels of CRH,ACTH,and CORT decreased in the EA group and FLX group(P＜0.05 or P＜0.01).Metabolomics results showed that compared with the CON group,87 metabolites were upregulated and 72 metabolites were downregulated in the MOD group.Compared with the MOD group,27 metabolites were upregulated and 23 metabolites were downregulated in the EA group.Differential metabolites were enriched using the KEGG pathway and it was found that the protective mechanism of EA may be related to metabolic pathways,purine metabolism,mTOR signaling pathway,valine,leucine,and isoleucine biosynthesis and endogenous cannabinoid pathway.Conclusion:EA can improve anxiety and depression like behavior in CSD mice via regulating the endogenous metabolites in the hippocampus.
Objective:To construct a diagnostic model for cardiac neurosis with rigid-deficiency symptoms using LASSO regression combined with Nomogram.Methods:A single-center prospective study was conducted to collect the clinical data of 141 patients with cardiac neurosis.Variables included in the analysis were age,ethnicity,marriage,education,mental/physical work,BMI,traditional Chinese medicine symptoms,and the scores of Chinese medicine five-state personality,the Hamilton Anxiety Inventory-14-item scale,the Hamilton Depression Inventory-24-item scale and the Symptom Checklist-90.The influencing factors significantly associated with the diagnosis of rigid-deficiency symptoms were screened by LASSO regression,incorporated into a binary multi-factor Logistic regression analysis to construct a diagnostic model,and the model was evaluated for predictive discrimination and calibration,internally validated using 10-fold cross-validation.Finally,the model was visualized by Nomogram,and the diagnostic threshold was determined according to the ROC curve.Results:A total of 70 patients with rigid deficiency syndrome and 71 patients with non-rigid deficiency syndrome were included.The LASSO regression screened the five most significant variables associated with the diagnosis of rigid-deficiency symptoms as female,age,fatigue,belching,and Shaoyang points in the five personality states.The model AUC was 0.85 and the H-L test was 2.94(P=0.982 4),suggesting good model differentiation and calibration,and the 10-fold cross-over internal validation results suggested an AUC of 0.82.Conclusion:In this study,the diagnostic model constructed by LASSO regression combined with Nomogram can help clinicians to rapidly diagnose patients with cardiac neurosis with rigid-deficiency symptoms,but the accuracy of the results needs to be further verified by large-sample clinical studies.
Objective:To observe the clinical effect of Tongdu Tiaoshen acupuncture(通督调神针刺法)combined with rehabilitation training on children with autism spectrum disorder.Methods:60 children with autism were divided into treatment group and control group according to random number table,with 30 cases in each group.Children in both groups received comprehensive rehabilitation training(behavior modification training,speech training,multi-sensory game training,structured teaching training),and the treatment group received Tongdu Tiaoshen acupuncture therapy.Both groups were treated once a day,5 times a week,for 4 consecutive weeks as a course of treatment,a total of 4 courses of treatment.Children's Autism Rating Scale(CARS),Autism Efficacy Rating Scale(ATEC),Autism Psychoeducational Rating Scale for Children(PEP-3),and Children's Functional Independence Scale(WeeFIM)were used to evaluate the two groups of children before and after treatment,and the clinical efficacy of the two groups was compared.Results:After treatment,the scores of ATEC factors(speech communication,social ability,sensory perception,behavior)in both groups of children decreased compared to before treatment(P＜0.05),while the scores of PEP-3 and the total score of WeeFIM increased compared to before treatment(P＜0.05).After treatment,compared with the two groups,the ATEC scores of various factors(speech communication,social ability,sensory perception,behavior)and the total CARS score of the treatment group were lower than those of the control group(P＜0.05),while the PEP-3 scores and the WeeFIM total score were higher than those of the control group(P＜0.05).The total effective rate of the treatment group was significantly higher than that of the control group(P＜0.05).Conclusion:The treatment of autism spectrum disorder with the combination of acupuncture therapy and rehabilitation training is more effective,can better improve the speech expression and social ability of children,and improve their quality of life.
