Background: Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis, because they have the capacity of self-renewal, and differentiation into spermatozoa. Freezing is the most common long-term preservation approach for SSCs. The present study aimed to investigate the cryoprotective impacts of FBS (Fetal Bovine Serum) and trehalose on caprine SSCs. Materials and Methods: SSCs were isolated from prepubertal goat testis by enzymatic digestion and differential plating. Cells were divided into 9 groups. The control group included SSCs without cryoprotective agents. In the treatment groups 1, 2, 3 and 4, concentration of 10% FBS, and various concentrations of trehalose (0, 50, 100 and 200 mM), and in the treatment groups 5, 6, 7, and 8, concentration of 20% FBS and various concentrations of trehalose (0, 50, 100 and 200 mM) were used respectively. The viability rate of the cells was assessed immediately after isolation, following addition of cryoprotectant agents and after thawing. Identification of cells was confirmed by immunocytochemical staining against PGP9.5 antigen. Data were analyzed using one-way ANOVA test. Results: The viability rate of SSCs in various treatments following addition of FBS and trehalose were similar to viable cells immediately after isolation. Furthermore, higher viability rates of SSCs after thawing were observed in freezing medium containing 10% FBS and 200 mM trehalose (P<0.05). Conclusion: The results revealed that freezing in 10% FBS with 200 mM trehalose acts as efficient method for the cryopreservation of caprine SSCs.
Background: Infertility is one of the most common human problems that affects the physical, mental and social health of individuals. This study aimed to determine the effectiveness of positive psychotherapy based on belief to good on the mental health and marital quality of infertile women. Materials and Methods: The current study was a quasi-experimental with a pretest-posttest design and follow-up with a control group. The statistical population of the study consisted of infertile women referring to Samen Medical Complex in Mashhad in 1398. Thirty people were selected voluntarily and randomly divided into experimental (N=15) and control (N=15) groups. The experimental group received treatment in 8 sessions of 90 minutes. Data were collected using the General Health Questionnaire (GHQ-28) and Revised Dyadic Adjustment Scales (RDAS) in three time periods. Data analysis was performed using SPSS-26 and repeated measures analysis of variance. Results: Positive psychotherapy based on belief to good has played a significant effect on mental health index (P>0.01) and marital quality (P>0.01). In addition, mental health outcomes were stable up to one-month follow-up (P>0.01). Conclusion: Positive psychotherapy based on belief to good is an effective way to increase mental health and improve the marital quality of infertile women.
Background: Acetyl salicylic acid (ASA) or Aspirin is one of the most widely used drugs in the world; one of its side effects is the male reproductive system alteration. The present study aimed to investigate the effect of moderate intensity aerobic exercise on ASA-induced reproductive system alteration in rats. Materials and Methods: In two stages, fifteen rats (N=15) were randomly divided into control (C, n=5), non-treatment (NT, n=5) and aerobic-treatment (AT, n=5) groups; the AT and NT groups used aspirin 12.5 mg/kg-daily/five weeks. The AT group ran at moderate intensity on a rodent-treadmill/four weeks, five sessions a week. Groups C and NT did not receive any intervention. Serum testosterone level, spermatogenesis indices and sperm parameters of rats were evaluated and the data were analyzed by one-way variance and Tukey post-hoc-test at alpha level 0.05 by SPSS. Results: Data statistical analysis showed that there was a significant difference between the groups in terms of serum testosterone level (P=0.02), sperm quantity (P=0.0001), viability (P=0.001), maturity (P=0.0001), sperm DNA fracture (P=0.002), TDI, SI and RI indices, tubule diameter (P=0.0001), tubular epithelium thickness (P=0.0001) and number of Leydig cells (P=0.003), but there was no significant difference between the groups in terms of sperm motility (P=0.07). Tukey post-hoc-test showed that these differences were between NT and AT groups, but no significant difference was observed between C and AT. Conclusion: It can be concluded that four-week moderate exercise helps accelerate the reproductive conditions recovery to just before taking acetyl salicylic acid (ASA) or Aspirin.
