前列腺癌的发病与基因异常变化密切相关,大量前列腺癌患者存在多种癌基因和抑癌基因突变.前列腺癌转基因动物模型的优势在于用重组 DNA 的方法导入外源基因后能自主调控基因表达,进而控制疾病特征.因此,该肿瘤模型与人体肿瘤的组织病理学及分子特征高度相似,其能模仿前列腺癌的发生、发展等病理过程,有望提高对前列腺癌的认识并为寻求新型治疗方法提供重要研究手段.现就常用的前列腺癌转基因动物模型进行介绍.
Objective:To explore the effects of minimally invasive hematoma removal at different times on neurological function and oxidative stress in patients with intracerebral hemorrhage（ICH）.Methods:A total of 82 ICH patients were randomly divided into observation group and control group, with 41 cases in each group.The control group was treated with minimally invasive hematoma removal in the early stage（6 to 24 hours after onset）,and the observation group was treated with minimally invasive hematoma removal in the ultra-early stage（within 6 hours after onset）.The patients in both groups were followed up for 6 months.The clinical efficacy, oxidative stress indexes [malondialdehyde（MDA）,superoxide dismutase（SOD）]before and after treatment, The hematoma clearance rate at 24 hours and 72 hours after operation, scores of NIHSS,CSS and ADL before and after treatment, complications within 6 months after operation were compared.Results:Six months after operation, the total effective rate of treatment in the observation group（97.56%） was significantly higher than that（82.93%） in the control group（P＜0.05）.After treatment, the MDA level of the two groups was lower than that before treatment, and the SOD level after treatment was higher than that before treatment（all P＜0.05）.After treatment, the MDA level in the observation group was significantly lower than that in the control group, and the SOD level after treatment was higher than that in the control group（all P＜0.05）.The NIHSS and CSS scores of the two groups after treatment were lower than those before treatment（all P＜0.05）.The NIHSS and CSS scores of the observation group were significantly lower than those of the control group after treatment（all P＜0.05）.The ADL score of the two groups after treatment was lower than that before treatment, and the ADL score of the observation group after treatment was significantly lower than that of the control group（all P＜0.05）.Six months after operation, the incidence of complications in the observation group（4.88%,2 cases/41 cases） was significantly lower than that in the control group（19.51%,8 cases/41 cases）,the difference was statistically significant（P＜0.05）.Conclusion:For ICH patients, compared with early surgery, minimally invasive hematoma removal at ultra-early stage（within 6 hours after onset） can reduce the oxidative stress response of patients and improve neurological function, with good safety.
Objective:To investigate the application value of microvascular decompression, transcranial electrical stimulation and balloon compression in the treatment of trigeminal neuralgia.Methods:Eighty-six patients with trigeminal neuralgia were selected and divided into group A（n=29）,group B（n=29） and group C（n=28） according to the random number table method.Group A was treated with microvascular decompression, and group B was treated with transcranial electrical stimulation, and group C was treated with balloon compression.The visual analogue scale（VAS） was used to evaluate the degree of pain.The clinical effects, VAS score, surgical and hospitalization information, oxidative stress level, and incidence of complications were compared among the three groups.Results:The total effective rate of group A was higher than that of group B and group C（all P＜0.05）.At 3 months after operation, the VAS score of group A was lower than that of group B and group C（all P＜0.05）.The hospitalization cost of group B was less than that of group A and group C,and the hospitalization time and operation time were shorter than those of group A and group C（all P＜0.05）.At 6 hours after operation, the levels of NE and Cor in group A were higher than those in group B and group C（all P＜0.05）.There was no significant difference in the incidence of complications between group A and group B and group C（all P＞0.05）.Conclusion:The clinical effect of microvascular decompression is more significant, the degree of pain after microvascular decompression is lower, the oxidative stress response is greater, the hospitalization cost of transcranial electrical stimulation is lower, the hospitalization time and operation time are shorter, but the intraoperative trauma is larger.Balloon compression as a minimally invasive surgery, the incidence of complications is not significantly different among the three groups.
目的:探究肉瘤滤过性毒菌致癌同源体B1(BRAF)V600F基因及生长分化因子 15(GDF-15)表达变化与甲状腺乳头状癌(PTC)患者淋巴结转移及预后的关系.方法:收集出现淋巴结转移的PTC患者 60 例作为研究组,收集同期无淋巴结转移的PTC患者 53 例作为对照组,均测定 BRAF V600 E基因及 GDF-15 表达.比较两组患者BRAF V600 E基因及 GDF-15 表达差异.分析两指标与研究组患者临床特征间的关系.分析两指标对 PTC 患者淋巴结转移的预测价值及与患者预后间的关系.结果:研究组患者 BRAF V600 E 基因突变率及 GDF-15 阳性表达率高于对照组(均P<0.05).BRAF V600 E基因突变及 GDF-15 阳性表达与淋巴结转移 PTC 患者肿瘤直径和是否包膜侵犯有关(均P<0.05).BRAF V600 E基因突变及 GDF-15 阳性表达对 PTC 患者淋巴结转移均具有一定的预测价值,两项联合的预测价值更高(均P<0.05).BRAF V600 E基因突变患者累积无进展生存率低于 BRAF V600E基因未突变患者,GDF-15 阳性表达患者累积无进展生存率低于 GDF-15 阴性表达患者(均P<0.05).结论:PTC患者BRAF V600 E基因及 GDF-15 表达变化与PTC患者淋巴结转移及预后关系密切,均可能参与了 PTC病情发生及进展过程,可以作为患者病情评估及预后的预测指标.
目的:探讨孕晚期孕妇产前抑郁的影响因素及抑郁程度与血清促甲状腺激素(TSH)、甲状腺过氧化物酶抗体(TPOAb)的相关性.方法:选取伴产前抑郁的孕晚期产妇 108 例为观察组,无产前抑郁的孕晚期产妇 216 例为对照组.采用临床自制问卷进行问卷调查并检测甲状腺功能指标[血清 TSH、游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、TGAb、甲状腺过氧化物酶抗体(TPOAb)].比较两组临床资料.采用 Logistic回归分析孕妇产前抑郁的影响因素,然后构建模型并分析模型的预测价值.比较两组甲状腺功能指标.采用 Pearson 法分析甲状腺功能指标与抑郁自评量表(SDS)评分的相关性.结果:观察组大专以下文化程度、不良妊娠史、睡眠质量差的比例高于对照组(均P<0.05).观察组中文版压力知觉量表(CPSS)总分高于对照组(P<0.05).观察组和对照组家庭人均月收入、婆媳关系和夫妻关系分布情况比较,差异有统计学意义(均P<0.05).家庭人均月收入、婆媳关系、夫妻关系、不良妊娠史、睡眠质量及CPSS总分是孕妇产前抑郁的独立影响因素(均P<0.05).观察组 TSH 低于对照组,TPOAb高于对照组(均P<0.05).TSH 与 SDS呈负相关(r=-0.682,P<0.05),TPOAb与 SDS呈正相关(r=0.538,P<0.05).结论:不良妊娠史、家庭收入、婆媳及夫妻关系、睡眠质量、CPSS总分是孕晚期孕妇产前抑郁的因素影响.血清TSH 和TPOAb水平与孕晚期孕妇产前抑郁程度相关.
