目的 探讨干扰素诱导蛋白(IFI 16、IFI 27、IFI 30)mRNA表达与系统性红斑狼疮(SLE)的关系.方法 以前期构建SLE患者外周血单个核细胞(PBMCs)基因表达系列分析(SAGE)文库为基础,分析该文库中出现的3个干扰素相关基因标签(tag),并采用反转录-聚合酶链反应(RT-PCR)方法,临床检测IFI16、IFI27、IFI30在43例SLE患者和25名正常对照人群PBMCs中的mRNA表达水平.结果 分别约有88%、98%、58%的SLE个体其IFI16、IFI27、IFI30 mRNA表达增加,高于正常对照组总体均数95%可信区间的上限.与正常对照组比较,SLE活动组和缓解IFI16、IFI27、IFI30的表达水平均增加(P值均＜0.01).活动组与缓解组间比较,活动组SLE患者高表达IFI16和IFI27(P=0.003,P=0.001),IFI30的表达差异无统计学意义(P=0.227). SLE患者IFI16、IFI27、IFI30间的表达水平互不相关.IFI16、IFI27表达与狼疮疾病活动指数(SLEDAI)呈正相关,相关系数分别为0.557、0.414,P直分别为0.000,0.006.IFI30表达与SLEDAI无相关(r=-0.102,P=0.514).结论 SLE患者IFI16、IFI27、IFI30表达增高,揭示其在SLE发病中的重要作用.

Objective To investigate the mRNA expression level of neurogranin on peripheral blood mononuclear cells(PBMCs)from patients with systemic lupus erythematosus(SLE).Methods Top 20 tags of SLE PBMCs SAGE library were searched from normal lymphocytes SAGE library including navie-T,Th1,Th2, CD8+T, NK and B cells,and their abundance was compared.The mRNA expression level of neuro-granin,a differential over-expressed tag,was detected in 35 cases of SLE and 15 normal controls by reversetranscription-polymerase chain reaction (RT-PCR).Results Neurogranin tag could only be detected in SLE PBMCs SAGE library,but was hardly found in normal lymphocyte SAGE library.However,either SLE pa-tients or normal controls showed a detectable mRNA level of neurogranin on PBMCs by RT-PCR.The mRNA level of neurogranin in active SLE patients was significantly increased than those in controls(P＜0.001).but only slightly increased in inactive SLE patients (P＞0.05).Conclusion Neurogranin,as a novel proapototic factor,is overexpressed on PBMCs of SLE patients.It may be involved in the regulation of abnormal immune responses in lupus.

系统性红斑狼疮(SLE)是内科疾病中病变损伤范围最为广泛的疾病之一.但皮肤黏膜病变程度多较轻,罕见严重的皮肤黏膜损害.我们成功救治1例SLE合并中毒性表皮坏死松解症(TEN),伴有重度消化道出血的病例,兹将临床资料报道如下.

This study examined the gene expression patterns of peripheral blood mononuclear cells (PBMCs) in patients with systemic lupus erythematosus (SLE) by using serial analysis of gene expression (SAGE) technology. Following the construction of serial analysis of gene expression (SAGE) library of PBMCs collected from 3 cases of familial SLE patients, a large scale of tag sequencing was performed. The data extracted from sequencing files was analyzed with SAGE 2000 V 4.5 software. The top 30 expressed genes of SLE patients were uploaded to http://david.niaid.nih.gov/david/ease.htm and the functional classification of genes was obtained. The differences among those expressed gene were analyzed by Chi-square tests. The results showed that a total of 1286 unique SAGE tags were identified from 1814 individual SAGE tags. Among the 1286 unique tags, 86.8% had single copy, and only 0.2% tags had more than 20 copies. And 68.4% of the tags matched known expressed sequences, 41.1% of which matched more than one known expressed sequence. About 31.6% of the tags had no match and could represent potentially novel genes. Approximately one third of the top 30 genes were ribosomal protein, and the rest were genes related to metabolism or with unknown functions. Eight tags were found to express differentially in SAGE library of SLE patients. This study draws a profile of gene expression patterns of PBMCs in patients with SLE. Comparison of SAGE database from PBMCs between normal individuals and SLE patients will help us to better understand the pathogenesis of SLE.

<正>系统性红斑狼疮(SLE)是一种具有家族聚集倾向的自身免疫性疾病,多基因参与疾病的发生已成共识。基因芯片研究提示其具有不同于正常健康个体的基因表达谱,但还不足以区分其他相关的自身免疫性疾病如类风湿关节炎。对此一个较为合理的解释是目前用于基因芯片研究的已知基因数量和特异性尚未达到将其区分的程度。然而,现阶段新基因发现已进入一个相对缓慢的平台期,有

Objective To better understand the clinical features of intestinal pseudo-obstruction (IPO) associated with systemic lupus erythematosus (SLE). Methods Ten cases of SLE with IPO collected from July 2001 to July 2005 were investigated, and 20 cases without IPO hospitalized in our hospital during the same period were chosen by 1:2 ratio as a control group. Other 40 cases previously reported from Chinese and English literature, together with the present cases, were reviewed. Results Among the 10 cases of SLE with IPO, one presented with IPO as the initial manifestation of SLE, 5 patients had urinary tract involvement, and 4 patients responded well to methylprednisolone and maintained stable. In the review, those patients accounted for 46%, 64%, 46%, respectively. Except for the relative higher leukocyte counts (8.1±5.2 vs 4.0±1.8, P<0.05) and lower SLEDAI scores (6.1±2.2 vs 9.6±1.8, P<0.01) in the lupus-related IPO group, there are no statistical difference in other parameters commonly used in laboratory examination, including hemoglobulin level, platelet counts, 24 hours urinary protein, anti-nuclear antibodies, autoantibody profiles and complement C3, C4 level, between the lupus-related IPO group and the controls. Conclusion IPO can be the first manifestation of SLE in some patients but it usually represents a complication. There is an apparent association between lupus-related IPO and urinary tract involvement. Administration of glucocorticoids and immunosuppressive agents such as cyclophosphamide (CTX) can improve the lupus-related IPO patient′s prognosis.