Objective . To analyze the distribution of human papilloma virus (HPV) infection in women with cervical lesions of different grades and analyze the relationship of high-risk HPV and cervical lesions in order to facilitate targeted prevention. Methods . The infection status of HPV subtype was statistically analyzed in patients who underwent colposcopy examination from April 2017 to June 2019. Results . The infection rate of HPV was 81.4% in chronic cervicitis, 82.9% in 1ow-grade squamous intraepithelial lesion (LSIL), 63.7% in HSIL (high-grade squamous intraepithelial lesion), and 50% in cervical squamous cell carcinoma (CSCC). Among the 16 high-risk HPV types, the top six HPV types with the comprehensive infection rates were HPV16 > HPV52 > HPV58 > HPV18 > HPV51 > HPV53 in turn, and the infection rates were 23.3%, 14.8%, 13.3%, 9.8%, 9.2%, and 8.8%, respectively. The infection rates of HPV16 in chronic cervicitis group, LSIL group, and HSIL group were significantly different. There was no significant difference in the injection rates of HPV52, HPV58, and HPV18 among the three groups. HPV infection rates were highest in the 31–40 years old group, followed by the 41–50 years old group. Conclusion . The distribution of different types of HPV varies in different tissue types, which can be used to develop relevant vaccines to achieve better prevention and treatment of cervical cancer.

目的 基于Survivin信号通路探究miR-373对宫颈癌HeLa细胞增殖、侵袭和粘附能力的影响.方法 将HeLa细胞随机分为3组,转染miR-373 inhibitor质粒为miR-373组,转染miR-NC空质粒为miR-NC组,未经任何质粒转染为空白对照组.实时荧光定量PCR(qPCR)检测细胞中miR-373、Survivin表达,MTT法检测细胞增殖能力,平板克隆实验检测细胞克隆形成能力,Transwell法检测细胞侵袭能力;基质胶粘附实验检测细胞粘附能力;蛋白质印迹检测细胞中Suivivin、caspase-3、caspase-9蛋白表达.结果 和空白对照组比较,miR373组细胞中miR-373和Survivin表达均显著降低(P<0.05);miR-373组24、48、72h时细胞增殖能力,侵袭能力及粘附能力均显著降低(P<0.05);miR-373组细胞Survivin蛋白表达显著降低,caspase-3和caspase-9蛋白表达显著升高(P<0.05).结论 下调miR-373表达可抑制宫颈癌HeLa细胞的增殖、侵袭和粘附能力,其机制可能与Survivin通路有关.

Objective To investigate the clinical value of hysteroscopy in helping identify the etiology of intrauterine fluid collection in postmenopausal women.Methods One hundred and thirtyfour postmenopausal women with intrauterine fluid collection,detected via transvaginal sonography,were admitted in the department of gynecology and obstetrics at Beijing ChaoYang Hospital from May 2014 to October 2016.All clinical data were retrospectively analyzed.All patients underwent hysteroscopy and biopsy of the endometrium.The results of hysteroscopy were analyzed against biopsy results.Results Hysteroscopy revealed that 116 of the 134 cases had uterine fluid(86.6％).Based on hysteroscopy results,44 (37.9 ％) had endometritis,10 (8.6 ％) had atrophic endometritis,5 (4.3 ％) had endometrial carcinoma,and 7(6.0 ％)had endometrial polyps,but the case numbers and rates for these conditions from microscopic examination were39 (33.6％),15 (12.9％),6 (5.2％),and 7 (6.0 ％),respectively.When microscopic examination was used as the gold standard,the sensitivities and specificities of hysteroscopy in the diagnosis of endometritis,atrophic endometritis,endometrial cancer and endometrial polyps were 82.0 ％ and 85.7 ％,66.7 ％ and 97.1％,80.0 ％ and 98.2 ％,and 100.0％ and 97.3％,respectively.The diagnostic coincidence rates were 84.6％,93.2％,94.4％ and 97.4％,respectively.Conclusions Postmenopausal women with intrauterine fluid collection shown on transvaginal sonography should receive hysteroscopy for early detection of endometrial lesions.

剖宫产,俗称剖腹产,是指在由于各种原因胎儿无法经阴道自然娩出,而由医生采取的一种经腹切开子宫娩出胎儿的过程.由于该手术伤口大,创面广,易产生术后各种各样的问题导致产妇恢复慢.正确的护理可以促进伤口尽快痊愈,但进入炎炎夏季,伤口感染、不愈合的风险增加,加强护理就显得尤为重要.

Objective To explore the relevant factors of macrosomia in order to own better antepartum prevention and obsterical measure.Methods Retrospective analysis was conducted in the total of 349 complete macrosomia cases in this hospital,the relevant factors of macrosornia( except diabetes melitus) were compare and analyzeed.Results There was significant correlation between macrosomia and age of pregnant woman,gestional weeks,body mass index.Conclusion In order to reduce the incidence rate of macmsomia,decrease the rate of uterine-recision delivery and save the life for the mother and the infant,some effective measures should be carried out for pregnant ladies who are foreseen to have a macrosomia.

Objective To discuss the clinical value of tubal repatency under hysteroscopy using excellent wire to diagnose and treat tubal unexpedite.Methods From July 2006 to July 2010,one hundred and sixty cases were devided into two groups.Nighty-one cases were treated by increasing press.another 69 cases were treated using excellent wire.Results Within the 91 cases with 182 tubes,there were 51 expedite tubes,66 partly obstructed tubes,65 obstructed tubes before the operation and 81 expedite tubes,52 partly obstructed tubes,49 obstructed tubes after increasing press.Within the 69 cases with 138 tubes,there were 29 expedite tubes,23 partly obstructed tubes,86 obstructed tubes before the operation and 129 expedite tubes,6 partly obstructed tubes,3 obstructed tubes after using excellent wire.There was significantly difference between the two groups (P=0.01).Conclusions Tubal repatency under hysteroscopy using excellent wire is very convenient and efficient.