The wheat TaNADP-ME1 gene was responsive to drought,salt and low temperature. In this research we successfully constructed the plant overexpression vector pCAME1 by recombinant technology,transformed the callus which was induced from the mature embryo of rice by agrobacterium-mediated method,acquired resistant callus by hy-gromycin and transformed plant by differentiation and regeneration on media. The 20 transgenic rice plants were ac-quired by PCR identification. This research laid a foundation for identification the function of TaNADP-ME1 gene.

焉耆黑蘑菇(Agaricus gennadii (Chot.et Boud.)P.D.Orton)是新疆特有优质野生食用菌,但目前还未人工驯化栽培.利用组织分离法分离菌种,并将焉耆黑蘑菇驯化成功.研究结果表明:焉耆黑蘑菇可以在青草(870 g/kg)为主料,麸皮(100 g/kg)、石灰(30 g/kg)为辅料的培养料上正常生长发育,接种到子实体采收约70～90 d,1 a可栽培2次.

The cultivation conditions of Pholiota squarrosa mycelium were optimized in order to enhance product efficiency and shorten cultivation cycle.The one-factor experimental design and Plackett-Burman design showed that the optimum carbon source was maltose,the optimum nitrogen source was yeast,the optimum pH was 5,and that the optimum temperature was 23 ℃.What was more,the main factors influencing mycelium growth were maltose,yeast and K2HPO4.The optimum medium for Pholiota squarrosa mycelium growth was identified as 35g/L maltose+1.5g/L yeast+0.5 g/L K2HPO4+0.5g/L KH2PO4+0.5g/L MgSO4by the experiments of the steepest ascent and orthogonal designs.On the optimum medium the colony diameter was increased from 6.11cm to 7.41cm.

The wheat TaNADP-ME2 was not only responsive to light but also to drought,salt and low temperature.The recombinant plant overexpression vector was constructed,transformed into rice and acquired transgenic rice plant.The vector was constructed by recombinant technology.The agrobacterium was transformed by freeze-thaw method.The callus induced from the mature embryo of rice was transformed by agrobacterium-mediated method.The transgenic rice was identified by hygromycin and PCR method.The results revealed that recombinant plant overexpression vector pSUE2 has been constucted succeedingly and transformed into EHA105.The 16 transgenic rice positive plants were acquired by the PCR detection from HPT and TaNADP-ME2 gene.This research laid a foundation for identification the function of TaNADP-ME2 gene in enhancing water use efficiency in drought tolerance.

[Objective] To establish a rapid DNA extraction method for the screening of transgenic rice.[Method] The kit method and fast method of two DNA extraction methods were compared,and were proved by Ta NADP-ME1 and Ta NADP-ME2 gene transgenic rice respectively.[Result] PCR was done by using the DNA which extracted by the rapid method as template,about 1 700 bp Ta NADP-ME2 gene and about 1 900 bp Ta NADP-ME1 gene were obtained respectively,and the strips were clear and bright,comparing with the kit method showed no significant difference.[Conclusion] The rapid DNA extraction method of this study is economic,simple and quick,suitable for the large transgenic samples detection.