Taking blueberry‘Bluecrop'as material,the natural pollination seed germination efficiency at different fruit development stages were studied.The results showed that the mature blueberry seed germination efficiency in medium composition of 1/2WPM+GA3 600mg/L was 32.0%;young seed germination efficiency at two steps germination medium composition of 1/2MS and 30 d development cultivate was 19.0%;the young seed from fruit development phases of 42~50 dseed development efficiency was 16.3%~19.0%.The development cultivate in two kinds of dispose(2±2)℃ 20 d,(25±2)℃ 10 d and (25±2)℃ 10 d,(2±2)℃ 20 d seed germination efficiency was 23.1% and 28.8% respectively.At fruit development stage of 45 d,the mature,young and stunting seeds was 52.1%,32.4% and 15.5% respectively.