The N-acetyl-carnosine eye drops were prepared,and was stable at room temperature for 2 years. No eye irritation was observed by the eye drops with rabbits as the animal models.Pharmacodynamic experiments of rat cataract induced by sodium selenite demonstrated that the eye drops could delay the development of lens turbidity, enhance superoxide dismutase,catalase,glutathione peroxidase activities and decrease malondialdehyde and infusible protein content of lenses.The effect of the middle dosage of the eye drops was comparable to the two positive control drugs pirenoxine sodium and bendazac lysine eye drops.