The effects of natural factors (temperatures +34°C and +20°C) and anthropogenic xenobiotics (1,2,4-TriH; 1-(CH3SO2)-1H-1,2,4-Tri; 4-(PhSO2)-4H-1,2,4-Tri; 4-(TolSO2)-4H-1,2,4-Tri) on watercress Lepidium sativum L. was studied. For this, 4 series of experiments were carried out. In the first two series, the effect of concentrations was studied: 0.001, 0.01, 0.1 mg/ml during germination at different temperatures for three days. It was found that the ability of the triazole compounds to inhibit the germination of watercress seeds increased with increasing concentration of the solutions. The magnitude of lipophilicity was the determining factor in their toxicity. Differences between the influence of the studied temperatures on the energy of seed germination weren’t revealed. The ability of the synthesized triazoles to inhibit the growth of watercress roots and stems increased with increasing their concentration. At the same time, their toxicity was significantly higher at the higher of the studied temperatures. The next two series examined the ability of Lepidium sativum to adapt to the toxicity of triazoles. To do this, the seeds were germinated during the day at contrasting temperatures in solutions with a concentration of 0.001 mg/ml, then they were germinated in solutions of 0.1 mg/ml for two days. It turned out that triazoles had significantly different effects on the development of the adaptive response in plants. Only 4-(PhSO2)-4H-1,2,4-Tri; 4-(TolSO2)-4H-1,2,4-Tri caused a significant development of an adaptive response, the magnitude of which also depended on the cultivation temperature. We found that the value of lipophilicity of the studied triazoles was of significant importance during their direct action, and the values of their molecular weight and molecular volume were important for the development of preadaptation.

Bacterial cellulose obtained by culturing Gluconacetobacter sucrofermentans in HS environment was converted to sulfonate derivatives using methane-, toluene- and 2-phthalimidoethanesulfonic acids in pyridine. When the ratio of the starting reagents is 1 : 1, the modification of bacterial cellulose according to the primary hydroxyl group of glucopyranose fragments is most likely. The formation of 6-substituted bacterial cellulose derivatives was observed in the reaction mixture. The IR spectra of the reaction products contain absorption bands, which are specific for (O–SO2) group in the region 1377-1338 cm−1 (as), 1178-1154 cm−1 (s), fragments of the corresponding sulfonic acids, as well as free hydroxyl groups of glucopyranose in the region 3495-3382 cm−1. Bacterial cellulose 2-phthalimidoethanesulfonate was dissolved in pyridine. After drying with a desiccant in a desiccator, it turned into a dense transparent film of brown color. The increased molecular film allows to explain the side reaction occurring between the oxo group and fragments of one of the chains of modified cellulose and the non-substituted hydroxymethyl group. The IR spectrum of bacterial cellulose 6-(2-phthalimidoethanesulfonate) contains absorption bands in the region 1711 cm−1, which are specific for (Ar–CO–O) group, and absorption bands in the region 1618 cm−1, which prove the presence of (CO–NH) group. In order to impart antibiotic properties to the bacterial cellulose 6-(2-phthalimido-ethanesulfonate) film, it was physically modified with clotrimazole. The obtained experimental data showed that the films subjected to treatment with a 1% solution of clotrimazole have antibacterial and antifungal effects and prevent the growth of pathogenic microbiota on the wound surface. The exit rates of clotrimazole from the bacterial cellulose 6-(2-phthalimidoethanesulfonate) film and from the pure bacterial cellulose film differed, but only slightly. 2-Phthalimidoethanesulfonate bacterial cellulose films can be used to form composites of effective wound covering, since in addition to the unique properties of bacterial cellulose itself (low allergenicity and adhesion to the wound surface, high hygroscopicity) they will have a regenerating effect.

В работе изучено влияние состава водно-ацетонитрильного элюента на сорбцию бензимидазолов октадецилсиликагелем в рамках модели Снайдера-Сочевинского. Методом линейного регрессионного анализа получены трехпараметрические уравнения, связывающие факторы удерживания с некоторыми физико-химическими характеристиками молекул бензимидазолов. На основании температурных зависимостей факторов удерживания рассчитаны стандартные изменения энтальпии процесса перехода бензимидазолов из водно-ацетонитрильной фазы в слой октадецилсиликагеля и энтропийные составляющие процесса; рассмотрены энтальпийно-энтропийные зависимости процесса сорбции.

The effect the composition of a water–acetonitrile eluent has on the sorption of benzimidazoles by octadecyl silica gel is studied using the Snyder–Soczewinski model. Three-parameter equations associating retention factors and certain physicochemical characteristic of the molecules of benzimidazoles are obtained via linear regression analysis. Standard changes in the enthalpy of the transfer of benzimidazoles from the water–acetonitrile phase to the layer of octadecyl silica gel and entropic components of the process are calculated from the temperature dependences of the retention factors. The enthalpy–entropy dependences of sorption are discussed.

We carried out the investigation of cellulose composites and composites with poly-N-N-dimethyl-3,4-methylenpyrrolidin chloride with different concentration of polymer. Cellulose was produced by Gluconacetobacter sucrofermentas. The data of Fourier spectral analysis and the change in weight of the films revealed the high efficiency of sorption of polymer on the bacterial cellulose. The presence of polymer decreases the swelling rate constant and increases the change in weight of the composite when placed in distilled water. Scanning electron microscopy confirmed the formation of the crystals of the polymer in dried films when placed them into a 15% solution of the polymer. The composites with poly-N-N-dimethyl-3,4-methylenpyrrolidin chloride show antibacterial properties, they suppress E.coli growth. Specimens treated by a 1.5% and 0.75% polymer solution are more effective.

We carried out the investigation of cellulose composites and composites with poly-N-N-dimethyl-3,4-methylenpyrrolidin chloride with different concentration of polymer. Cellulose was produced by Gluconacetobacter sucrofermentas. The data of Fourier spectral analysis and the change in weight of the films revealed the high efficiency of sorption of polymer on the bacterial cellulose. The presence of polymer decreases the swelling rate constant and increases the change in weight of the composite when placed in distilled water. Scanning electron microscopy confirmed the formation of the crystals of the polymer in dried films when placed them into a 15% solution of the polymer. The composites with poly-N-N-dimethyl-3,4-methylenpyrrolidin chloride show antibacterial properties, they suppress E.coli growth. Specimens treated by a 1.5% and 0.75% polymer solution are more effective.

The results of a chromatographic study of sorption of several benzotriazole derivatives on octadecyl silica gel were reported. The physicochemical and electronic parameters of benzotriazoles were calculated. The effect of the structure of analyte molecules and eluent composition on chromatographic retention of these substances was analyzed.

Mesomorphic, sorption, and selective properties of a three-component sorbent based on a mixture of nematic (N) liquid crystals of 4-methoxy-4′-ethoxyazoxybenzene (MEAB) and 4,4′-diethoxyazoxybenzene (azoxyphenetol, AOP) of an eutectic composition and heptakis-(2,3,6-tri-O-benzoyl)-β-cyclodextrin (Bz-β-CD) are studied. For 30 organic compounds of different classes with linear and cyclic molecular structures, including optical isomers of limonene, pinene, camphene, and butanediol-2,3, thermodynamic functions are determined for their gas-phase sorption using a three-component MEAB-AOP-Bz-β- CD sorbent (62: 28: 10 wt %). It is found that the investigated sorbent possesses high structural selectivity (αp/m = 1.128–1.059, 100–130°C, N) and moderate enantioselectivity (1.07–1.02) within a broad temperature range (95–170°C) including both mesomorphic and isotropic phases of the sorbent. It is shown that the enantioselectivity of the sorbent is apparent under conditions of both increasing retention when a chiral Bz-β-CD additive is introduced into the MEAB–AOP system (limonenes, pinenes, camphenes) and decreasing retention (butanediols-2,3).

Thermodynamics of the sorption of some benzimidazole derivatives on octadecyl silica gel from aqueous-acetonitrile solutions is studied under reverse-phase high-performance liquid chromatography conditions. The chromatographic-retention-property correlation dependences are considered.

Nucleoside-5´-phosphates and N,N´-(thio)carbonylbisazoles interaction was studied for the evaluation of nucleoside-5´-phosphorazolide-2´,3´cyclo(thio)carbonate formation possibility by the semi-empirical method PM6 of SCIGRESS Modeling 3.0.0 software. Azole moiety involves imidazole fragment. It was shown that formation of nucleoside-5´-phosphorazolide2´,3´-cyclo(thio)carbonates is energetically less efficient than formation of nucleotide-5´-phosphorazolides.

Полуэмпирическим методом PM6 с использованием программы SCIGRESS Modeling 3.0.0 изучено взаимодействие нуклеозид-5´-фосфатов с N,N´-(тио)карбонилбисазолами c целью оценки вероятности образования нуклеозид-5´-фосфоазолид-2´,3´-цикло(тио)карбонатов. Азольная компонента представлена фрагментом имидазола. Показано, что процесс образования нуклеозид-5´-фосфоазолид-2´,3´-цикло(тио)карбонатов энергетически менее выгоден, чем нуклеозид-5´-фосфоазолидов.

Изучена термодинамика сорбции некоторых производных бензимидазола в условиях обращенно-фазовой высокоэффективной жидкостной хроматографии (ОФ ВЭЖХ) на октадецилсиликагеле из водно-ацетонитрильного раствора. Рассмотрены корреляционные зависимости “хроматографическое удерживание-свойство”.

Проведен сравнительный анализ антибактериальной активности вновь синтезированных производных адамантана и бензимидазола и биологически активных пептидных соединений, выделенных из гемолимфы личинок восковой моли. Анализ показал, что антибактериальные пептиды в концентрации 10-15 мкг/мл обладают наибольшим бактерицидным эффектом по отношению к Escherichia coli по сравнению с синтетическими антибиотиками.

Dose-response stady (concentration of 50-200 mkg/ml) effect on growth total degidrogenase, catalase, sukcinatdegidrogenase activity of E. COLI phenolic benzimidazole derivatives containing a methyl group and the glycoside radical is researched. All compounds have dozo-respouse bacteriostatic effect and cause metabolic stress, resulting in higher concentrations of active degidrogenase and catalase. Most damaging effect has 4(2-methyl-1fi-benzimidazol-ylmethyl)phenyl-2,3,4,6-tetra-O-azethyl-/3-D-glucopyranozyd in a concentration of 200 mkg/ml.

Good bioactivities urea have attached the attention of chemical experts. The groups with peptide bond or similar parts are considered as basic framework that can restrain the activities of enzyme. The urea compound containing the -CONH radicle have the extensive living creature activity.In this thesis, five diarylurea derivatives were synthesized by reacting substituted phenylaniline with isocyanate. IR and HNMR are used to analyze its component element. According to the results ,we can verify its structure and identify.

The synthesis of dibazole derivatives: 2-(2-benzyl-1H-benzimidazole-1-ylmethyl)phenol, 4-(2-benzyl1H-benzimidazole-1-ylmethyl)phenol, 4-(2-benzyl-1H-benzimidazole-1-ylmethyl)phenyl-2,3,4,6-tetra-O-acetyl-β-D-glucopyranoside was implemented. The structure of the compounds was confirmed by IR and 1 H NMR spectroscopy. Using the program HyperChem 7.52 we built the models of molecules of synthesized derivatives of benzimidazole and calculated their physicochemical properties. According to the estimation of the alleged biological activity by the program PASS Professional 2007 the synthesized compounds may possess some types of biological action useful in pharmacology.