Markers of aging known today do not clearly define the biological age of the organism, which creates the need for their further search. The oral fluid is a convenient object for such studies, since it contains proteins whose conformation corresponds to the age of the organism. The method developed by the authors allows, by transferring biological fluids into a solid phase, to transform the structure of the molecular-level components dissolved in them to a level accessible to visual analysis. The aim of the study is to search for markers of biological age in the solid-phase structures of the oral fluid. Materials and methods. The structures of the solid phase oral fluid of 240 people with a sanitized oral cavity were studied. There are four age groups of 60 patients each: middle age; elderly; senile and long-livers. Oral fluid was taken in the morning on an empty stomach. The method of cuneiform dehydration of biological fluids was used. Dehydration of drops of oral fluid was carried out on special test cards under standard conditions. As a result, dry films (facies) were obtained, the structural features of which were examined by microscopy. Results. The structure of oral fluid facies is determined by the ratio of salt and organic substances present in it. During the formation of a facies, salts bind to proteins that have an abnormal conformation. In middle-aged patients, the facies have a clear division into saline and marginal (protein) zones. With age (elderly and senile age), due to the increased activity of catabolic processes, in the facies of the oral fluid, an increasing area is occupied by organic substances, and the areas occupied by salts decrease, while the facies lose their physiological structuring. At the same time, the facies of the oral fluid of most long-livers retain a structure similar to the facies of middle-aged representatives. Conclusions. The biological age of the organism is determined by the level of its autointoxication by proteins with pathological conformation. Autotoxins are contained in the oral fluid and are manifested in the structure of its facies. Due to natural age-related metabolic shifts towards an increase in catabolic processes, the protective systems that block autointoxication by metabolites cease to provide their complete neutralization, which is reflected in the facies structure. Facies of the oral fluid of long-livers have a fundamentally different structure compared with the facies of the elderly and senile persons, indicating a low degree of autointoxication of the organism of long-livers, which determines a longer life span. Thus, specific solid phase structures of oral fluid facies can be used as objective markers of biological age.

It is noted that the desire to be immortal is the most important, strongest and oldest, alas, unrealizable dream of a person. A brief review of the history of the fight against human aging in different countries in different eras is given, as well as an analysis of the theoretical justifications for the causes and mechanisms of aging, the development of means and methods for combating old age. It is noted that the universal "geroprotector" is systematic work in its various physical and intellectual variants. It is shown that only the combined efforts of society, the state and the individual can optimally solve the problem of longevity of the country's population.

Introduction . Currently, chronic kidney disease is called a "silent killer", since patients with the development of a pathological process do not experience pain for a long time, modern functional and laboratory diagnostic methods also do not allow to determine the early signs of this disease, which contributes to the chronicity of the disease. Purpose : to reveal the hidden signs of the pathology of the urinary system according to the structures of the solid phase of the patient's urine. Materials and methods . The urine of 359 patients without signs of renal pathology was studied. The method of analysis of solid-phase structures of dehydrated urine (facies) was applied by microscopy in ordinary and polarized light. Processing the structures of the solid phase of urine with a stream of steam of boiling water caused their recrystallization, which made it possible to reveal hidden markers of renal pathology. Results . Analysis of the structure of native urine facies (N-facies) showed that in all examined patients they were completely covered with salt crystals, which corresponded to the physiological state of the body. Steam jet treatment of urine N-facies revealed organic (non-mineralized) inclusions in 23 patients, and a marker of bacteriuria was detected in 9 patients. The study of urine facies with the Lithos protein reagent (LR-facies) showed that the Lithos-reagent protein dissolves organic inclusions and they cease to appear in LR-facies. In 15 patients, organo-mineral microaggregates were detected in the marginal zone of the LR-facies of urine. After exposure to steam, these microaggregates disappeared in 7 patients, and remained in 8. In 9 patients, in whose N-facies, after steaming, a marker of bacteriuria was manifested, in the LR-facies of urine of 7 patients, a marker of candidiasis was detected. Conclusion . The study of the structure of urine facies before and after their treatment with boiling water steam makes it possible to identify hidden forms of renal pathology: insufficient filtration capacity of the kidneys, the risk of nephrolithiasis, bacterial and fungal infection. The data obtained make it possible to ensure timely prevention and treatment in the early stages of the disease.

Актуальность. Важным патогенетическим звеном развития осложнений при введении наркоза является нарушение дыхания в виде апноэ, которое может переходить в агонию. Вместе с тем, не существует работ по регистрации мозгового кровотока в период агонии. Несмотря на многочисленные результаты исследований на животных, демонстрирующих, что общая анестезия приводит к нейродегенерации и когнитивным нарушениям, на сегодняшний день чёткого консенсуса о влиянии анестетиков на человека всё ещё не достигнуто. Цель исследования: выявить маркёры стадий наркоза у экспериментальных животных по структурам твёрдой фазы сыворотки крови (СК) и показателям мозгового кровотока (МК). Материалы и методы. Исследования проведены на половозрелых беспородных крысах-самцах. Животные наркотизировались хлоралгидратом: контрольные группы получали дозу 400 мг/кг, в/б, а опытные - 500-600 мг/кг, в/б. В разные фазы действия наркоза регистрировали системное артериальное давление (АД), МК, частоту дыхания и частоту сердечных сокращений. МК регистрировали прибором ЛАКК-02 (ЛАЗМА). АД измеряли монитором MEC8 Mindray. Структуры твёрдой фазы СК животных исследовали методом краевой дегидратации на тест-картах диагностического набора «Литос-система». Микроскопию структур СК проводили в поляризованном свете при увеличении × 200 и × 400 (микроскоп DM2500 фирмы «Leica»). Результаты. В контрольных группах показатели МК, частоты дыхательных движений и частоты сердечных сокращений достоверно не различались. В опытных группах через один час после дачи повышенной дозы наркоза наблюдали снижение частоты сердечных сокращений, частоты дыхания и показателей АД (р < 0,05). Сразу после начала апноэ МК начинал быстро снижаться вслед за падением АД. Амплитуда сердечного ритма в спектре колебаний МК повышалась в три раза по сравнению с контролем. От начала апноэ время снижения МК до нуля составляло от 60 до 240 с. До введения наркотического средства структуры СК были представлены в виде сферолитов и веерных образований, которые характерны для физиологического состояния организма. В стадии апноэ сферолиты и веерные структуры СК были испещрены множеством мелких изотропных включений, а также имели единичные мелкие и обширные изотропные разломы. В агональной стадии большинство структурных образований СК было представлено фрагментами сферолитов с многочисленными полосками и ветвистыми образованиями с высокой анизотропией, множественными разломами с изъеденными границами. Заключение. Динамика морфофункциональных показателей экспериментальных животных в результате воздействия наркотизирующих средств определяется перестройкой, возникающей в структуре белковых молекул, строение которых визуализируется при переводе СК в твёрдую фазу. Особенности патологических структур твёрдой фазы СК указывают на глубину наркотизации организма. Эти изменения согласуются с динамикой показателей сердечно-сосудистой и дыхательной систем экспериментальных животных после наркотизации. Результаты указывают на возможность применения анализа структур твёрдой фазы СК при ретроспективной оценке динамики МК, глубины наркотизации и побочных эффектов, вызываемых различными наркотизирующими средствами у пациентов с последующим использованием этих данных для выбора вида и оптимальной дозы наркотизирующего вещества при предстоящих хирургических вмешательствах. Background. An important pathogenetic step in the development of anesthesia complications is a breathing disorder in the form of apnea, which may transform into agony. However, there are no reports of cerebral blood flow (CBF) during the period of agony. Despite numerous animal studies showing that general anesthesia leads to neurodegeneration and cognitive impairment, there is still no clear consensus on effects of anesthetics in humans. Aim of the study. To identify markers of anesthesia stages in experimental animals by structures of blood serum (BS) solid phase and by indexes of CBF. Materials and methods. The study was carried out on sexually mature outbred male rats. The animals were anesthetized with chloral hydrate: control groups received a chloral hydrate dose of 400 mg/kg, i.p., and experimental groups received 500-600 mg/kg, i.p. Blood pressure (BP), CBF, respiratory rate and heart rate were recorded during different phases of anesthesia. CBF was recorded with a LAKK-02 device (LAZMA); BP was measured with a MEC8 Mindray monitor. BS solid phase structures were studied using the method of marginal dehydration on test cards of the “Litos-system” diagnostic kit. BS structures were examined under a microscope in polarized light at a magnification of ×200 and ×400 (DM2500 microscope, Leica). Results. In the control groups, indexes of CBF, respiratory rate, and heart rate did not significantly differ. In the experimental groups, one hour following administration of the increased anesthetic dose, the heart rate, respiratory rate, and BP were decreased (p < 0.05). Immediately after the onset of apnea, the drop in blood pressure was followed by a rapid decline in CBF. The heart rate amplitude in the spectrum of CBF oscillations was increased three times compared to the control. The time from the apnea onset to zero CBF ranged from 60 to 240 s. Before the drug administration, the BS structures represented spherulites and fan-shaped formations characteristic of the physiological state of the body. During the apnea stage, BS spherulites and fan-shaped structures were dotted with many small isotropic inclusions, and also had single small and extensive isotropic faults. During the agonal stage, most of the BS structural formations represented fragments of spherulites with numerous stripes and branched formations with high anisotropy and multiple faults with corroded boundaries. Conclusion. The changes in morpho-functional indexes under the action of anesthetics are determined by structural rearrangement of protein molecules as visualized during the BS transformation into the solid phase. The features of BS solid phase pathological structures indicate the anesthesia depth. These changes are consistent with changes in cardiovascular and respiratory parameters in anesthetized animals. The results indicate a possibility of using the analysis of the BS solid phase structures for a retrospective assessment of changes in CBF, the depth of anesthesia, and side effects caused by various anesthetics in patients. Subsequently, these data may be used for selecting the anesthetic type and its optimum dose for upcoming surgical interventions.

The effects of platinum nanoparticles on the morphological structures of the solid phase of blood serum and the tone of cerebral microvessels as indicators of the dynamics of homeostasis were studied on outbred albino male rats (n=40) weighing 300-350 g. Platinum nanoparticles were injected to experimental animals in 1 ml of physiological saline. For systemic BP measurements and blood sampling, the femoral artery was isolated and catheterized. The study of solid phase structures of blood serum was conducted by the method of cuneiform dehydration. The results showed that injection of platinum nanoparticles significantly affected the body of experimental animals.

Актуальность. Долголетие человека с высоким качеством жизни является одной из наиболее важных проблем мирового значения. Долгожители представляют собой биологическую элиту человеческой популяции, что заставляет проводить углублённые исследования факторов, обеспечивающих физиологическое старение и долголетие человека. Одним из таких факторов является способность организма нейтрализовать токсические свойства метаболитов посредством минерализации. Цель: выявить биологические особенности организма долгожителей по критерию соотношения показателей эндогенной интоксикации и минерализации эндотоксинов по сравнению с пациентами среднего, пожилого и старческого возраста. Материалы и методы. Материалом для исследования являлись биологические жидкости (сыворотка крови, моча, ротовая жидкость). В качестве основного применён метод клиновидной дегидратации биологических жидкостей. Как вспомогательные использованы методы: определение молекул средней массы в сыворотке крови; определение высокомолекулярной фракции циркулирующих иммунных комплексов в сыворотке крови и метод рентгеноспектрального микроанализа структур твёрдой фазы мочи и ротовой жидкости. Результаты. Установлено, что естественное возрастное увеличение количества токсических метаболитов в организме человека значительно опережает повышение нейтрализующей токсины биоминерализационной активности. Однако пациенты старше 85 лет представляют исключение из этой закономерности: рост биоминерализационной активности в данной возрастной группе адекватен росту эндогенной интоксикации. Это обеспечивает более высокую резистентность организма к токсическим метаболитам и определяет большую продолжительность жизни. Заключение. Структура фаций биологических жидкостей даёт возможность интегрально оценивать текущей уровень эндогенной интоксикации в соотношении с биоминерализационной активностью организма. Background. Longevity with a high quality of life is one of the most important goals of global significance. Centenarians represent a biological elite of the human population, which encourages conducting in-depth studies of factors that provide physiological aging and longevity of people. One of these factors is the body’s ability to neutralize toxicity of metabolites through mineralization. Aim. To identify biological characteristics of a long-liver’s body by the criterion of intoxication to endotoxin mineralization ratio as compared to patients of middle, elderly, and senile age. Methods. Cuneiform dehydration of biological fluids was used as the main method. Auxiliary methods included determination of middle molecules in blood serum; determination of high-molecular fraction of circulating immune complexes in blood serum; and the x-ray spectral microanalysis of urine and oral fluid. Results. The natural, age-related increase in the number of toxic metabolites in the human body significantly precedes the increase in toxin-neutralizing biomineralization activity. However, centenarians (patients older than 85) are an exception to this pattern: in this age group, the growth of biomineralization activity is adequate to the growth of endogenous intoxication. This provides a higher resistance of the body to toxic metabolites and determines a longer life expectancy. Conclusion. The structure of facies of biological fluids allows integral evaluation of the current level of endogenous intoxication in relation to the biomineralization activity in the body. Long-livers were shown to have a higher ability to neutralize endotoxins through biomineralization, which provides them with a longer lifespan.

Актуальность. Проблема гипоксии представляет важный аспект в медицине. При этом вопросы глубинного влияния гипоксии на организм остаются во многом не изученными. Цель исследования состояла в оценке патофизиологических изменений организма экспериментальных животных в условиях острой гипобарической гипоксии (ОГГ) с разных позиций: с позиций базового (молекулярного) уровня - по твёрдофазным структурам сыворотки крови, и с позиций центрального управления жизнедеятельностью организма - по показателям мозгового кровотока. Методы. Для опосредованной оценки данных о трансформации структурно-энергетических параметров белковых молекул сыворотки крови (СК) в условиях гипоксии использован метод клиновидной дегидратации биологических жидкостей. Для оценки мозгового кровотока в условиях гипоксии использован вейвлет-анализ колебаний церебрального кровотока. Результаты. Исследование действия ОГГ на крыс методом структурного анализа твёрдой фазы СК и методом регистрации локального мозгового кровотока показало разделение животных по уровню устойчивости к гипоксии на две группы. Первая группа при гипобарии равной высоте 11,5 км быстро входила в агональное состояние, однако при этом сохраняла значительную часть энергетических ресурсов белковых молекул, что устанавливалось по системной организации и локальным структурам твёрдой фазы сыворотки крови, а также подтверждалось достаточно высоким уровнем активности мозгового кровотока. Вторая группа животных, при тех же условиях гипоксии, входила в агональное состояние значительно позднее, при этом отмечалось значительные изменения структуры и почти полное истощение энергетических ресурсов белковых молекул, и выраженное падение показателей мозгового кровотока. Однако у животных второй группы после перевода их в условия нормобарической оксигенации наблюдалось более быстрое восстановление активности микроциркуляции. Заключение. В основе патофизиологических изменений организма экспериментальных животных при ОГГ лежит трансформация структур молекулярного уровня, наблюдаемых как по системной организации, так и локальным структурам твёрдой фазы СК. Экспериментальные животные имеют различный порог устойчивости по отношению к ОГГ. Background. The issue of hypoxia is an important aspect of medicine. However, the profound effect of hypoxia on the body remains largely unexplored. The aim of the study was to evaluate pathophysiological changes in experimental animals under the conditions of acute hypobaric hypoxia (AH) from different aspects: from the aspect of basic (molecular) level - by solid-phase structures of the blood serum (BS), and from the aspect of the central management of the body - by cerebral blood flow. Methods. Cuneiform dehydration of biological fluids was used for an indirect assessment of the transformation of structural and energy parameters of BS protein molecules in the hypoxic conditions. The wavelet analysis of cerebral blood flow oscillations was used to evaluate cerebral blood flow in hypoxia. Results. As a result of the study, the rats were divided into two groups based on their resistance to hypoxia. The first group faster developed the agonal state at a simulated altitude of 11.5 km. At the same time, these rats retained a significant part of the energy resources of protein molecules, which was established by the systemic organization and local structures of the BS solid phase and confirmed by a sufficiently high rate of cerebral blood flow. The second group of animals exposed to the same hypoxic conditions developed the agonal state much later and showed significant changes in the structure and almost complete depletion of energy resources of protein molecules associated with a marked drop of cerebral blood flow. However, the animals of the second group showed a faster recovery of microcirculation after return to the normobaric conditions. Conclusion. Pathophysiological changes in the rat body exposed to AH are based on transformation of molecular structures evident from both the systemic organization and local structures of the BS solid phase. The experimental animals have different thresholds of resistance to AH.

Актуальность. Среди урологических заболеваний одним из ведущих является нефролитиаз. Существует несколько теорий его возникновения, однако ряд аспектов, касающихся вероятных причин его возникновения и механизмов развития, остаются актуальными для исследования и в настоящее время. Цель: представить динамику нефролитиаза по структурам твёрдой фазы мочи с интерпретацией диагностического значения основных морфологических образований. Методы. Формирование твёрдофазных структур мочи осуществлялось методом клиновидной дегидратации (технология «Литос-Система»). Количественное распределение химических элементов в различных зонах фаций мочи проводилось методом рентгеноспектрального микроанализа. Результаты. Технология исследования твёрдофазных структур мочи позволяет диагностировать процесс камнеобразования в почках, определять степень его активности и состав камнеобразующих солей мочи. Показано, что активность нефролитиаза определяется степенью сродства (прочностью связей) камнеобразующих солей с молекулами белка. Заключение. Изучение структур твёрдой фазы мочи у больных нефролитиазом позволило получить качественно новую диагностическую информацию о взаимодействии двух основных составляющих мочи - минеральной и органической. Механизм возникновения и развития нефролитиаза объясняется формированием аномально прочных молекулярных взаимосвязей между органической и минеральной составляющей мочи, что создаёт условия для появления патологических органо-минеральных агрегатов. Метод клиновидной дегидратации биологических жидкостей позволяет обнаружить эти агрегации в виде макроструктур, доступных для визуального анализа. Основное клиническое значение технологии «Литос-Система» состоит в её возможности чёткого контроля за течением нефролитиаза и формированием наиболее эффективных терапевтических программ. Aim. Among urological diseases a leading one is nephrolithiasis. There are several theories of its occurrence; however, studying many aspects of probable causalities and developmental mechanisms is still relevant. Objective: to determine the dynamics of nephrolithiasis by structure of urine solid phase and to interpret the diagnostic value of major morphological formations. Methods. Urine solid phase structures were formed using the method of cuneiform dehydration (Lithos-System technology). Quantitative distribution of chemical elements in different urine facie zones was determined using the X-ray microanalysis. Results. The technology of studying urine solid-phase structures allows to detect the process of stone formation in the kidneys and to detemine the process intensity and the composition of urinary stone-forming salts. It was shown that nephrolithiasis activity was determined by the degree of affinity (bond strength) of stone-forming salts to protein molecules. Conclusion. Studying structures of the urine solid phase in patients with nephrolithiasis provided qualitatively new diagnostic information about the interaction between two main components of urine, the mineral and organic ones. The mechanism for nephrolithiasis onset and development can be attributed to formation of abnormally strong molecular relationships between organic and mineral components, which creates conditions for emergence of pathological organo-mineral aggregates. The method of cuneiform dehydration of biological fluids allows detecting these aggregates in the form of macrostructures available for visual analysis. The main clinical value of the Litos-System technology is its ability to accurately monitor the course of nephrolithiasis and to support development of most effective therapeutic programs.

Reversible cerebral ischemia of medium severity was reproduced in male Wistar rats by bilateral occlusion of the common carotid arteries. Solid-phase structures (anisomorphons) were obtained by marginal dehydration of the serum. Small focal isotropic defects in the serum anisomorphon texture were found in 100% cases during occlusion of the carotid arteries. Similar signs were detected in all patients with chronic cerebral ischemia, which proved specificity of this morphological marker of the disease.

Цель проведённого исследования состояла в оценке эффективности визуального анализа твёрдофазных структур спинномозговой жидкости для диагностики ранних форм нейросифилиса. Методы. Использован метод краевой дегидратации биологических жидкостей, входящий в состав авторской диагностической технологии «Литос-система». Диагностика раннего асимптомного нейросифилиса заключается в выявлении деструктивных образований в форме овалов в морфологической картине твёрдой фазы спинномозговой жидкости. Результаты. Проведён сравнительный анализ результатов исследования спинномозговой жидкости у 19 больных с подтверждённым диагнозом «ранний асимптомный нейросифилис», полученных традиционными лабораторными методами и методом краевой дегидратации. Выявлено, что локализация овалов внутри сферолитов указывает на длительность заболевания нейросифилисом менее трёх лет, а вне сферолитов - от трех до пяти лет. Заключение. Метод краевой дегидратации позволяет диагностировать ранний асимптомный нейросифилис по наличию деструктивных образований в форме овалов в морфологической картине твёрдой фазы спинномозговой жидкости. The aim of this study was to evaluate effectiveness of visual analysis of solid-phase structures in cerebrospinal fluid to diagnose early forms of neurosyphilis. Methods. We used a method of marginal dehydration of biological fluids as a part of the author’s diagnostic technology, Litos-System. Early asymptomatic neurosyphilis is diagnosed based on detection of destructive, oval-shaped formations in the morphological picture of cerebrospinal fluid solid phase. Results. Data from analyses of cerebrospinal fluid performed with traditional laboratory methods and the method of marginal dehydration were compared for 19 patients with documented diagnosis of early asymptomatic neurosyphilis. A localization of ovals within spherulites indicated a less than a three-year duration of neurosyphilis while a localization outside spherulites indicated a duration of three to five years. Conclusion. The method of marginal dehydration allows detecting early asymptomatic neurosyphilis based on the presence of destructive, oval-shaped formations in the morphological picture of cerebrospinal fluid solid phase.

The purpose. To reveal solid-phase structures of blood serum during and after experimental cerebral ischemia. Methods. Integrated estimation pathophysiological changes at brain ischemia received on solid-phase structures of blood serum (BS) at 27 rats-male Vistar in weight of 300‒390 g. Reversible brain ischemia of moderate severity was reproduced imposing surgical clips on both common carotids for 15 minutes then clips removed and provided bloodstream restoration on carotids. Blood took away in Eppendorf´s test tube in volume on 1 ml before imposing clips, directly ahead of their removal and in 30 minutes after bloodstream restoration. By the Method cuneiform dehydration received BS facia (dry a film) which structures analyzed by means of stereomicroscope MZ12 firm Leica. Results of researches fixed on structural parameters BS before imposing clips, after removal clips and in 30 minutes after bloodstream restoration. Results.Before ischemia the integrated picture facias BS rats had the harmonious system organisation: the radial or partially-radial arrangement of the cracks, accurately generated konkrecias that testified to normal physiological condition of experimental animals. During the period occlusions carotids there were markers of a ischemia, activation of a capillary blood-groove, inflammatory reaction and stress. Partially these signs remained at reperfusion. Conclusion. The conducted researches of structures BS on experimental animals have allowed to reveal marker structures BS similar revealed at inspection of people with an ischemia of a brain.

The purpose:The purpose. To reveal solid-phase structures of blood serum during and after experimental cerebral ischemia.Methods:Integrated estimation pathophysiological changes at brain ischemia received on solid-phase structures of blood serum (BS) at 27 rats-male Vistar in weight of 300-390 g. Reversible brain ischemia of moderate severity was reproduced imposing surgical clips on both common carotids for 15 minutes then clips removed and provided bloodstream restoration on carotids. Blood took away in Eppendorf`s test tube in volume on 1 ml before imposing clips, directly ahead of their removal and in 30 minutes after bloodstream restoration. By the Method cuneiform dehydration received BS facia (dry a film) which structures analyzed by means of stereomicroscope MZ12 firm «Leica». Results of researches fixed on structural parameters BS before imposing clips, after removal clips and in 30 minutes after bloodstream restoration.Results:Before ischemia the integrated picture facias BS rats had the harmonious system organisation: the radial or partially-radial arrangement of the cracks, accurately generated konkrecias that testified to normal physiological condition of experimental animals. During the period occlusions carotids there were markers of a ischemia, activation of a capillary blood-groove, inflammatory reaction and stress. Partially these signs remained at reperfusion.Conclusion:The conducted researches of structures BS on experimental animals have allowed to reveal marker structures BS similar revealed at inspection of people with an ischemia of a brain.

The effects of various modes of low-intensity laser exposure on the sera of healthy subjects and patients are studied. It is shown by the cuneiform dehydration method that the serum structure is liable to change in response to certain modes of low-intensity laser exposure. The structure of patients’ sera is more sensitive to low-intensity laser exposure.

A literature review of the issues pertinent to etiology and pathogenesis of chronic pyelonephritis is presented. Different aspects of pathogenesis of the disease in selected age groups of the patients (from childhood to senility) are considered and analysed with special reference to the etiology of acute and chronic pyelonephritis as well as the risk factors of this disease.

The difficulty of early diagnostics of weakly symptomatic clinical course of chronic pyelonephritis (CP) is attributable to the lack of adequate methods. We used a novel Litos system diagnostic technology based on morphological analysis of dehydrated biological fluid The aim of the study was to develop methods for CP diagnostics from morphological characteristics of urine at different stages of the disease. It included 108 patients aged 58+/-1.3 yr with CP and 24 practically healthy subjects. Standard examination of all patients was supplemented by the analysis of urine using the Litos system. It was shown that morphological picture of urine makes it possible to objectively detect not only complete remission or exacerbation of the disease but also partial remission and high risk of CH even in patients with weakly symptomatic clinical course of this pathology. In case of incomplete remission, the treatment requires adequate long-term (three-course) antibacterial therapy despite normal results of urine analysis and microbiological studies. Patients at high risk of CP need to be prescribed preventive phytotherapy.

The article reviews present-day pathogenetic and diagnostic conceptions and methods of diagnosis of urolithiasis. Its medication and prevention methods are outlined.

A complex of methods for early diagnosis of urolithiasis is proposed. This complex includes ultrasonic investigation of the kidneys and new diagnostic technique Litos-System which allow making diagnosis before formation of a renal calculus.

The efficacy of the plant diuretic Diuress is evaluated by using the rapid diagnosis program including renal ultrasonography, Lithos system, and urinary sediment microscopy.

The process by which biological fluids are crystallized makes it possible to identify rhythmic undulatory oscillations occurring in the liquid phase of the medium. Pathological changes in the body impair physiological rhythms at different levels, these impairments being reflected in the biocrystalline structure of a biological fluid during its self-organization. Targeted modifications of the rhythm characteristic of a biofluid may help to evaluate the resistance of the organism to environmental factors.