Classical swine fever virus (CSFV) is a virulent pathogen that affects the global swine industry. Classical swine fever caused by CSFV is highly lethal and transmissible. Current attenuated vaccines present challenges, such as biosafety concerns and limitations in differential diagnosis. In previous study, we successfully constructed transgenic rice strains, E2-1 and E2-2, expressing the CSFV E2 antigen using a high-efficiency bioreactor system based on rice endosperm. This platform provides a safe and cost-effective approach for large-scale oral subunit vaccine production due to the protein-storage properties of rice endosperm and the absence of contamination risks from animal pathogens. In this study, systematic tracking of T1–T3 plant generations confirmed stable integration and continuous expression of the E2 gene, as verified by PCR and Western blot, with antigen titers in seeds remaining stable up to 2^7. Agronomic traits analysis revealed that the transgenic lines and the low-gluten rice variety showed significant improvements in plant height, grain number per spike, grain chalkiness, and other key indexes, thereby maintaining strong cultivation adaptability. Environmental safety tests demonstrated that the transgenic lines E2-2were more suitable for E2 vaccine production and posed no risk of genetic drift to surrounding weeds. This study is the first to report assessments of genetic stability, agronomic performance, and environmental safety of the CSFV E2 antigen expressed in multiple rice generations, which lays a key technical foundation for the commercial development of plant-derived swine fever vaccine.

IntroductionAutophagy is an important process in host cell responses to viral replication and spread, including those against infectious bursal disease virus (IBDV). Programmed death-1 (PD-1) is a known immunoinhibitory receptor, and its expression causes immune dysfunction in B lymphocytes, resulting in increased progression of immunosuppressive diseases. However, the role of PD-1 in autophagy during IBDV infection remains unclear.MethodsWe investigated the mechanism by which chicken PD-1 regulates autophagy during IBDV infection.ResultsIBDV infection enhanced PD-1 expression in chicken tissues and DT-40 cells. Subsequent interaction analyses revealed that PD-1 interacted only with the viral protein VP2 to enhance the IBDV replication in DT-40 cells. PD-1 overexpression significantly increased IBDV-induced autophagy, whereas silencing of PD-1 had the opposite effect in IBDV-infected DT-40 cells. Furthermore, PD-1 enhanced the activation of FoxO1 via the PI3K/AKT pathway. Finally, we demonstrated that autophagy is critical for role of PD-1 in regulating VP2 protein expression and IBDV titers.DiscussionThese findings present a novel mechanism wherein PD-1 induces autophagy by activating the PI3K/AKT/FoxO1 pathway to facilitate IBDV replication, providing a new avenue in developing universal vaccine adjuvants for IBDV infection control.

Based on the previously verified head-to-tail dimer vaccine model, a dimer of the HN protein (defined as Osr2HN) expressed in rice endosperm was designed. In previous immunization experiments in chickens, immunization with two doses (0.5 µg, equivalent to 1/127 of a grain of rice) or a single dose (5 µg) provided complete protection. To advance the commercialization process of this product, in this study, we selected two transgenic rice strains (HN-1 and HN-2) and cultured them for three generations to evaluate their genetic stability, agronomic traits, and safety. Insertion site analysis showed that exogenous genes were stably integrated into nuclear chromosomes with no variants, as confirmed by PCR, qRT-PCR, and Western blotting. The transgenic strains exhibited germination rates, growth cycles, and 12 agronomic traits similar to those of the wild-type TP309, though HN-2 showed increased chalkiness. Pollen viability remained unchanged, and no transfer of the HN gene to weeds was detected. Field biodiversity analysis revealed no impact of the HN gene on pest and weed communities. These findings validate the transgenic rice’s genetic stability, agronomic adaptability, and environmental safety, providing critical data to support the acceleration of its commercialization as a plant-derived vaccine platform.

Efficient eradication of cancer cells and enhanced tumor accumulation of drug via immune cells represent promising strategies for cancer treatment, yet they remain significant challenges. Inspired by neutrophils (NEs), we designed a novel nano-regulator (OMV@PCB NPs) to improve cancer therapy through polarizing NEs into anti-tumor N1 phenotype and photodynamic therapy (PDT)-assisted NEs hitchhiking delivery. OMV@PCB NPs were constructed by encapsulating Ce6 and SB525334 (TGF-β inhibitor) within PLGA nanoparticles, which were subsequently camouflaged with bacteria outer membrane vesicles (OMVs). Beyond inducing ROS generation under light irradiation to kill tumor cells, OMV@PCB NPs could effectively hitchhike NEs to enhance tumor accumulation based on PDT-assisted NEs recruitment. Encouragingly, SB525334, a TGF-β inhibitor in OMV@PCB NPs, could significantly polarize NEs into anti-tumor N1 phenotype, which could not only promote neutrophil elastase (ELANE) release to selectively eliminate cancer cells, but also elevate anticancer immunity by reversing the immunosuppressive tumor microenvironment, thereby achieving effective immuno-photodynamic therapy against tumor growth. This study offers a prospective avenue for enhancing cancer therapy.

Porcine reproductive and respiratory syndrome (PRRS), caused by PRRS virus (PRRSV), is a highly contagious disease with high morbidity and mortality that affects the global swine industry. Despite efforts to control it, there is still a widespread dissemination of PRRSV with obvious genetic variations in swine population, resulting in huge economic losses annually. Consequently, accurate laboratory diagnosis is crucial for the rapid confirmation of PRRSV infections. An immunoperoxidase monolayer assay (IPMA) was developed for the specific and sensitive detection of PRRSV based on a broad-spectrum anti-PRRSV monoclonal antibody (mAb) 28F6. The mAb 28F6-based IPMA could specifically detect PRRSV and possessed no cross-reactions with CSFV, PCV2, and PEDV. Sensitivity analysis showed that the limit of detection of the IPMA reached 10− 2.25 TCID50/100 µL. There was no significant difference in the detection of PRRSV of different passages with different batches of mAb 28F6, indicating that the IPMA had excellent repeatability. Additionally, the IPMA was capable of detecting multiple PRRSV variants, including field strains (e.g., BJ-4, HN07-1, and HNhx) and vaccine strains (e.g., HuN4-F112, JXA1-R, TJM-F92, GDr180, VR2332, CH-1R, and R98). Validation of the IPMA with qRT-PCR showed 100

The H9N2 avian influenza virus (AIV) is difficult to prevent and control because of its low pathogenicity and frequent mutation. In a previous study, the HA (hemagglutinin) protein of H9N2 was expressed in a rice endosperm reactor and prepared into a subunit vaccine to immunize chickens and mice, both of which exhibited a good immunity effect. The results of the intermediate tests of the transgenic strains (AIV-1 and AIV-3) showed that the HA gene can be stably expressed. Agronomic traits, such as plant height and number of grains, were significantly optimized in the transgenic strains. Moreover, no exogenous HA genes were found in the leaves of the weeds, and it was initially determined that there was no risk of gene drift. This study provides key technical support for the commercialization of plant subunit vaccines for avian influenza viruses.

Among various pathogens, viruses pose significant threats to the livestock and poultry industry, resulting in substantial annual costs due to production losses and vaccination. The MHC-I presentation pathway is a crucial surveillance mechanism for preventing viral infections. Consequently, many viruses have evolved sophisticated strategies to inhibit the presentation of viral peptides by MHC-I to CD8+ T-cells, thereby evading the immune system. Understanding the mechanisms that suppress the MHC-I pathway and identifying specific binding peptides are essential for comprehending viral immune evasion and developing effective animal vaccines. This review summarizes the viral strategies for evading immune recognition, including the inhibition of MHC-I molecules synthesis, degradation, transport, and assembly, which affect MHC-I surface expression during viral infections. We also present evidence that MHC-I surface expression is frequently lost during numerous viral infections in livestock and poultry and offer new insights into the underlying mechanisms through which viruses inactivate the MHC-I antigen presentation pathway. Collectively, these advanced findings on viral evasion from the MHC-I pathway could inform the development of more effectives strategies to restore immunological control over viral infections and improve vaccines for the livestock and poultry industry.

IMPORTANCE:The programmed death-1 (PD-1)/programmed death ligand-1 (PD-L1) pathway transmits negative immunoregulatory signals. Blocking this pathway using peptides or antibodies can restore immunity. OBJECTIVE:To evaluate the immune function of epitope peptides interacting with porcine PD-1 or PD-L1. METHODS:We optimized and synthesized peptides (PD-L14QN-GF and PD-L14QN-AF) using the solid-phase method and assessed their effects on peripheral blood mononuclear cell (PBMC) proliferation and PD-1 and cytokine expression after porcine reproductive and respiratory syndrome virus (PRRSV) infection in vitro and on antibody responses to a porcine circovirus type 2 (PCV2) vaccine in vivo. RESULTS:The optimized peptides PD-L14QN-GF and PD-L14QN-AF exhibited lower binding free energy and higher stability when interacting with the PD-1 target protein. Under both PRRSV-infected and non-infected conditions in vitro, both peptides enhanced the proliferation of PBMCs, inhibited PRRSV RNA replication, and downregulated PD-1 transcription levels. Additionally, PD-L14QN-GF and PD-L14QN-AF upregulated the mRNA transcription and protein secretion of interleukin (IL)-2, IL-10, and interferon-γ to varying degrees. In vivo experiments demonstrated that PD-L14QN-GF significantly increased the antibody titer and seroconversion rate of the PCV2 vaccine. CONCLUSIONS AND RELEVANCE:PD-L14QN-GF and PD-L14QN-AF induced stronger immune responses than PD-L14. PD-L14QN-GF has potential as an immune-enhancing adjuvant.

Intranasal vaccines hold a huge promise for preventing influenza infection, however, their development is compromised due to mucosal barriers and limited systemic and mucosal immunity. Herein, a novel biomimetic nano-adjuvant based on NK cell membrane (MNK)/MnO2 hybrid nanoparticles (NLipoMn) was developed for intranasal administration of H1N1 inactivated influenza vaccine (IIV). NK cell membrane, served as TLR4 agonist was fused with cationic liposomes encapsulating STING agonist MnO2 NPs (LipoMn) to yield NLipoMn, which inherited versatile characteristics to prolong the nasal retention of IIV. Encouragingly, CCL20 secretion was enhanced by NLipoMn-activated the TLR4/NF-kappa B pathway in mucosal endothelial cells, thereby promoting submucosal dendritic cells (DCs) recruitment for IIV uptake. Moreover, NLipoMn-IIV synergistically harnessed TLR4 and STING signaling pathway to augment STING activation though NLipoMn-activated the TLR4/NF-kappa B pathway, which demonstrated a superiority to elicit strong DCs maturation, CD8+ T cells activation, and high levels of antigen-specific IgG, antigen-specific IgA, cytokines secretion, resulting in a robust systemic and mucosal immune responses. Notably, intranasal immunization with NLipoMn-IIV elicited an effective immune protection against homologous and heterologous H1N1 influenza virus infection. This study provides a promising adjuvant candidate to develop needle-free intranasal vaccines that are active against influenza and other respiratory viral infection.

Background Porcine reproductive and respiratory syndrome (PRRS), caused by porcine reproductive and respiratory syndrome virus (PRRSV), is a highly contagious disease with high morbidity and mortality that affects the global swine industry. So far, there is still a widespread dissemination of PRRSV with obvious genetic variations in swine population, resulting in huge economic losses annually. Therefore, accurate laboratory diagnosis is needed to quickly confirm PRRSV infection. Results An immunoperoxidase monolayer assay (IPMA) was developed for the specific and sensitive detection of PRRSV based on a broad-spectrum anti-PRRSV monoclonal antibody (mAb) 28F6. The mAb 28F6-based IPMA could specifically detect PRRSV and possessed no cross-reactions with CSFV, PCV2, and PEDV. Sensitivity analysis showed that the limit of detection of the IPMA reached 10− 2.25 TCID50/100 µL. There was no significant difference in the detection of PRRSV of different passages with different batches of mAb 28F6, indicating that the IPMA had good repeatability. In addition, the IPMA could recognize a number of PRRSV variants including field strains such as BJ-4, HN07-1, and NADC30-like strain, as well as vaccine strains like HuN4-F112, JXA1-R, TJM-F92, GDr180, VR2332, CH-1R, and R98. Validation of the IPMA showed that it was in 100% consistency with qRT-PCR on the detection of 108 clinical samples. Conclusions The IPMA could meet the demand for the specific and sensitive detection of PRRSV, which is helpful for accurate monitoring and early warning of PRRSV infections.

The African swine fever virus (ASFV) continues to pose significant economic and pandemic risks. Consequently, discovering new, efficient vaccines is crucial. Messenger RNA (mRNA) vaccines have emerged as promising candidates, providing minimal risk of insertional mutagenesis, high safety profiles, effectiveness, rapid scalability in production, and cost-effectiveness. In this study, we have developed an ASF p30 mRNA vaccine candidate (mRNA/Man-LNP) employing mannose-modified lipid nanoparticles (LNPs). The mRNA/Man-LNP exhibited effective antigen presentation and facilitated dendritic cells (DCs) maturation. Notably, it elicited strong IgG titers and activated CD4+ and CD8+ T-cells in immunized mice, all while adhering to stringent biosafety standards. This investigation demonstrates that mRNA/Man-LNP can trigger both humoral and cellular immune responses, suggesting its potential as a potent and promising vaccine candidate for controlling African swine fever (ASF).

Programmed cell death protein 1 (PD-1)/PD-1 ligand 1 (PD-L1) binding contributes to immune evasion mechanisms responsible for B lymphocyte exhaustion and apoptosis. This facilitates immunosuppression in chronic viral infections, including infectious bursal disease virus (IBDV). Our previous study showed that PD-1 and PD-L1 expression increases in the peripheral blood mononuclear cells of chickens infected with IBDV. However, due to their high production costs and immune-related adverse events, monoclonal antibodies targeting PD-1 or PD-L1 are unsuitable therapeutic agents. Thus, in the current study, we designed peptides with optimized binding sites for PD-1 and investigated their ability to disrupt PD-1/PD-L1 binding and restore B lymphocyte function in vitro. The peptide gCK-16 exhibited a high affinity for PD-1 (KD: 3.37 nM) and effectively inhibited the PD-1/PD-L1 interaction in vitro. Moreover, gCK-16 significantly enhanced B lymphocyte proliferation. Remarkably, gCK-16 treatment abrogated the IBDV-induced upregulation of PD-1/PD-L1, NF-κB activation, and B lymphocyte apoptosis. Additionally, IBDV infection attenuated PI3K/AKT pathway activation in B lymphocytes, while gCK-16 treatment increased immunoglobulin M (IgM) production in IBDV-infected B lymphocytes. Together, these results demonstrate that gCK-16 treatment can potentially enhance B lymphocyte function against IBDV infection, guiding the development of vaccine adjuvants to effectively prevent IBDV-induced avian immunosuppression.

猪瘟(classical swine fever,CSF)是危害猪健康的重要传染病之一,具有急性、热性和高度接触性等特性,给世界养猪业造成了巨大的经济损失.PCR-ELISA 检测方法是在PCR和 ELISA 基础上创新的一种新技术,其灵敏性和准确性都高于单纯的 PCR 检测和ELISA检测.根据GenBank中猪瘟石门株序列,设计特异性引物,分别在 5'端标记生物素和地高辛,经过PCR扩增后,加入到包被有链霉亲和素的ELISA反应板上,然后通过DIG-HRP抗体进行显色反应,建立PCR-ELISA检测方法.结果表明:PCR-ELISA 最低检测到 1.81×104 拷贝/μL,普通 PCR 最低检测到 1.81×106 拷贝/μL,因此 PCR-ELISA 灵敏性比普通PCR高约 100 倍;对 27 份未知样品进行检测,普通 PCR 检测出阳性样品 15 份,阳性率为55.5%,PCR-ELISA检出 20 份阳性样品,阴性 7 份,阳性率为 74%,阳性检出率增加 18.5%;用建立的Real-Time PCR进行验证,检测结果与 PCR-ELISA 一致,两者的符合度为 100%;然后用PK15 细胞对普通PCR 未检测出的阳性样品进行病毒分离培养,通过间接免疫荧光(IFA)及普通PCR检测,结果显示该 5 份样品均为阳性.说明本研究所建立的 PCR-ELISA方法具有灵敏、特异、准确、快速、安全等优势,可应用于 CSFV 的快速诊断,为 CSFV 的流行病学监测提供有力保障.

To establish an indirect competitive enzyme-linked immunosorbent assay(ic-ELISA)method for the detection of zilpterol(ZIL)residues,in this study,based on the obtained rabbit anti-ZIL polyclonal an-tibody serum and the prepared complete antigen as the coating source,an ic-ELISA method for the detec-tion of ZIL residues was established by optimizing the reaction conditions;The results showed that the best dilution ratio of rabbit serum was 1:6 000,and the best antigen coating concentration was 2 μg/mL,the half inhibition concentration(IC50)is 0.317 ng/mL,the linear detection range(IC20-IC80)is 0.136-1.181 ng/mL,and the minimum detection limit(LOD,IC10)is 0.081 ng/mL.After optimization,the optimal pH of the detection system is 7.4,the salt concentration is≤0.4 mol/L,the content of methanol,ethanol,ace-tone and DMSO is≤5％,the content of acetonitrile is≤10％,and the concentration of bovine urine in the detection matrix is≤15％.The detection method has good stability and specificity.The addition recovery rate of intra-batch and inter-batch repeated test samples is 101％-1 12％and 101％-120％respectively,and the coefficient of variation is less than 10％,The correlation coefficient between the test results and HPLC-MS test results is R2=0.990 6.The detection method is fast,sensitive,accurate,reproducible and low detec-tion limit,and is suitable for high-throughput detection and on-site rapid screening of ZIL residues.

The African swine fever (ASF) pandemics pose a significant threat to the global swine industry, and the development of safe and effective vaccines is a daunting but necessary challenge. The level and persistence of immunity are very important for the effectiveness of the vaccine. Targeting antigens to antigen presenting cells (APCs) can greatly enhance immunogenicity. In this study, we developed a self-assembled nano-ASFV vaccine candidate (NanoFVax) targeting DCs, by covalently coupling the self-assembled 24-mer ferritin with the dominant B and T cell epitopes of the highly immunogenic ASFV antigen (p72, CD2v, pB602L and p30) and fused with the chemokine receptor XCL1 (a DC targeting molecule) through the SpyTag/SpyCatcher protein ligase system. Compared to monomeric protein, the nanoparticle vaccines can induce a more robust T-cell response, and the high-level antibody response against ASFV can last for more than 231 days. Therefore, the NanoFVax is a novel and promising vaccine candidate for ASFV.

本试验旨在筛选和鉴定与猪程序性死亡因子1(PD-1)及其配体(PD-L1)相互作用的表位多肽,为阻断猪PD-1/PD-Ls通路逆转机体的免疫功能提供新策略.根据已解析人与鼠的PD-1与PD-L1相互作用的关键氨基酸位点信息,分析猪PD-1与PD-L1蛋白相互结合的关键氨基酸位点,在关键氨基酸位点处设计系列表位多肽,固相合成法合成表位多肽;分离自然感染猪圆环病毒2型(PCV2)仔猪的外周血单个核细胞(PBMC),检测表位多肽与猪重组蛋白PD-1、PD-L1和PBMC的结合能力,选取能结合猪PD-L1和PBMC的表位多肽pPD-15,检测其对猪PBMC增殖的影响,分析其作为佐剂免疫后对小鼠猪瘟病毒(CSFV)抗体水平的影响,最后,高效液质联用色谱法(HPLC-MS)检测表位多肽pPD-15的纯度和氨基酸序列正确性.结果显示,表位多肽pPD-15能结合猪PD-L1和PBMC;增殖试验显示,pPD-15组M1的平均荧光强度(74.20％)比空白对照组M1的平均荧光强度(4.37％)提高了 69.83％,表明表位多肽pPD-15可促进猪PBMC的增殖;动物免疫试验显示,pPD-15作为佐剂可以提高CSFV抗体水平;HPLC-MS结果显示,合成的表位多肽pPD-15纯度高,氨基酸序列正确.本试验证实,表位多肽pPD-15具有提高体内外免疫力的能力,为研制新型免疫调节佐剂提供了科学依据.

Background The programmed death-1/programmed death-ligand-1 (PD-1/PD-L1) pathway transmits the negative immunoregulatory signals, leading to immunosuppression. Blocking the PD-1/PD-L1 pathway with peptides or antibodies can reverse the function of exhausted T cells, which can be a reference for developing studies on treating viral immunosuppressive diseases. Therefore, this work was developed to analyze the immune function of epitope peptides interacting with porcine PD-1 and PD-L1.Results After optimization, the proliferation percentages of PD-L14QN-GF and PD-L14QN-AF on PBMCS were 45.33%±6.16% and 56.20%±4.94%, respectively, which were increased by 14.7% and 25.8%. The inhibition rates of PD-L14QN-AF on PRRSV and PD-1 were 56.1.8% and 74.8%, which were increased by 35.0% and 29.4% compared with PD-L14, respectively. The inhibition rates of PD-L14QN-GF on PRRSV and PD-1 were 43.8% and 65.3%, which were 22.7% and 20.4% higher than those of PD-L14, respectively. The expression levels of IL-2 and IFN-γ in the PD-L14QN-GF group were 2.1 times and 2.8 times higher than those in the PD-L14 group and 1.2 times and 1.5 times higher than those in the PD-L14QN-AF group, respectively. The protein secretion levels of IL-2 and IFN-γ in the PD-L14QN-GF group were 2.5 times and 1.7 times higher than those in the PD-L14 group and 1.5 times and 1.2 times higher than those in the PD-L14QN-AF group, respectively. Furthermore, the PD-L14QN-GF and PD-L14QN-AF exhibited better immune effects than PD-L14. At 14 days after immunization, the antibody-positive rate in the PD-L14QN-GF group reached 80%, which was 30% and 50% higher than that in the PD-L14 group and normal group, respectively. The antibody titer in the PD-L14QN-GF group was 1.5 and 2 times higher than that in the PD-L14 and the normal groups, respectively.Conclusion PD-L14QN-GF was proved to be of high potential to develop immune-enhancing adjuvant.

为制备达氟沙星(DAN)多克隆抗体,建立检测DAN残留的间接竞争ELISA(ic-ELISA)方法,采用混合酸酐法制备免疫原DAN-BSA和包被原DAN-OVA,用DAN-BSA免疫新西兰大白兔获得DAN多抗血清,建立检测DAN的ic-ELISA检测方法.结果显示,成功合成了DAN-BSA和DAN-OVA,并获得DAN的多克隆抗体;用该抗体建立的ic-ELISA检测方法的半数抑制浓度为0.39μg/L,最低检测限为0.04μg/L,检测范围为0.09μg/L～6.16μg/L.结果表明,成功制备了抗DAN的多克隆抗体,并建立了检测DAN的ic-ELISA方法,为进一步开发DAN快速检测试剂盒奠定了基础.

为建立一种检测猪圆环病毒3型(porcine circovirus type 3,PCV3)的PCR-ELSIA方法,根据GenBank上报道的PCV3全基因组序列,针对其高度保守区域设计1对特异性引物,上、下游引物的5'端分别标记生物素和地高辛.经PCR扩增获得大量带有标记的目的基因,依靠生物素-链霉亲和素的非共价结合作用固定目的基因于载体上,再通过羊抗DIG-HRP的显色反应,建立PCR-ELISA检测方法.结果表明,该方法与猪繁殖与呼吸综合征病毒、猪瘟病毒、猪细小病毒和猪圆环病毒2型无交叉反应,特异性良好.PCR-ELISA方法最低检测限为5.58×102 copies/μL,敏感性比常规PCR高100倍(常规PCR的检测限为5.58×104 copies/μL).PCR-ELISA方法批内和批间重复性检测的变异系数分别为1.90％～5.20％和3.89％～9.03％.对132份临床样品进行PCV3检测,PCR-ELSIA检出率为9.10％(12/132),高于常规PCR的检出率(4.55％,6/132).综上,该方法具有特异、灵敏、安全高效的优点,可为PCV3的诊断、防控和流行病学调查提供新的技术手段.

目的 研究lncRNA SIL与肺纤维化过程中肺泡上皮细胞的增殖和迁移之间的关系.方法 利用RNA fish研究在转化生长因子(TGF-β1)诱导肺泡上皮细胞向间质细胞转化的过程中lncRNA SIL的表达变化,构建lncRNA SIL真核表达载体,转染A549细胞后,通过RT-PCR、MTT、Western blot、细胞损伤修复以及免疫荧光实验检测过表达lncRNA SIL对细胞A549增殖和迁移的作用以及对相关标志蛋白表达的影响.结果 当使用TGF-β1诱导48 h后,细胞中ln-cRNA SIL的表达量与对照组相比降低;在A549细胞中过表达lncRNA SIL时,细胞增殖能力、迁移能力以及细胞增殖相关蛋白PCNA和Ki67的表达与对照组相比均降低;对细胞标志蛋白的表达分析结果显示,与对照组比,转染lncRNA SIL组,间质细胞标志蛋白α-SMA和Collagen-1表达降低,肺泡上皮细胞标志蛋白E.cad表达升高.结论 LncRNA SIL可以通过抑制A549细胞的增殖和迁移达到抑制肺泡上皮细胞向间质细胞转化的作用.