目的 探讨木犀草素对慢性不可预知性温和应激抑郁模型(CUMS)所致小鼠脑组织氧化应激损伤的改善作用.方法 建立小鼠慢性不可预知性温和应激抑郁模型；用经典的糖水消耗实验检测小鼠快感缺失；旷场实验、悬尾实验和强迫游泳实验检测小鼠的活动能力和绝望程度；采用分光光度法测定小鼠脑组织超氧物歧化酶(SOD)和谷胱甘肽(GSH)活性及丙二醛(MDA)含量.结果 与模型组相比,木犀草素能明显改善小鼠抑郁样行为,提高SOD和GSH活性,降低小鼠脑组织MDA含量.结论 木犀草素对CUMS小鼠的抑郁样行为有改善作用,作用机制可能与其增强小鼠脑组织的抗氧化活性,改善氧化/抗氧化平衡有关.

目的 研究姜黄素对慢性不可预知性刺激诱发小鼠肾损伤的保护作用及机制.方法 采用慢性不可预知性刺激诱发小鼠肾损伤模型,经5周慢性不可预知性刺激诱发小鼠肾损伤,检测小鼠血清肌酐(Scr)、血清尿素氮(BUN)的含量、肾组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活性,谷胱甘肽(GSH)、丙二醛(MDA)的含量,观察肾病理组织形态学变化.结果 与慢性不可预知性刺激诱发小鼠肾损伤模型组相比,姜黄素组可明显降低小鼠血清中Scr和BUN的含量,降低肾组织中MDA的含量,提高肾组织中SOD和CAT的活性、提高GSH的含量.同时光镜发现模型组小鼠肾小管损伤明显,肾小球体积增大,内皮细胞肿胀脱落,间质炎性浸润,给予姜黄素后肾组织损伤明显降低.结论 姜黄素对慢性不可预知性刺激诱发的小鼠肾损伤具有保护作用,其作用机制可能部分来自于清除自由基和抑制脂质过氧化过程.

研究丁螺环酮衍生物对小鼠焦虑作用的研究。方法:应用小鼠应激性直肠升温、爬梯试验,观察丁螺环酮衍生物抗焦虑的药效和维持作用时间。结果:与对照组比较,丁螺环酮衍生物(1mg/kg)组直肠温度均显著降低(P<0.05);后肢站立数、站立时间与对照组比较,差异具有统计学意义(P<0.05),丁螺环酮衍生物总爬梯数与对照组比较,差异具有统计学意义(P<0.05)。结论:丁螺环酮衍生物对小鼠具有明确的抗焦虑作用。

Objective To observe the effect of low dose naloxone on analgesia of butorphanol. Methods 80 Kunming Jimpy mice were divided into 2 groups. 40 accepted hot-plate method and another 40 accepted stretching method. Each group was randomly divided into 4 groups furtherly: Group BS (butorphanol combined with sodium chloride) and group BN1, group BN2, group BN3 (butorphanol combined with naloxone 100 ng/kg, 10 ng/kg and 1 ng/kg respectively). Pain threshold in hot-plate test ( HPPT) and the number of stretching times were observed. Results Compared with group BS, HPPT of Jimpy mice in group BN2 increased significantly at 20 and 30 min(P 0.05)and the number of stretching times of Jimpy mice in group BN2 reduced significantly (P 0.05). Conclusion Low dose naloxone can enhance the analgesic effect of butorphanol, and the dose of 10 ng/kg has the best effect.

OBJECTIVE To observe the influence of low-dose naloxone(N)on the pain-killing effect of opium receptor(partial)agonist.METHODS The mice were divided into the three main groups of fentanyl(F),remifentanil(R)and butorphanol(B)and each group was divided into 4 sub-groups:F+NS group,F+N(100,10,1 ng)group,R+NS group,R+N(100,10,1 ng)group and B+NS group,B+N(100,10,1 ng)group.Hot plate test and stretching experiment were used to observe the effect on the pain threshold in hot-plate test(HPPT)and times of stretching of mice which used the above opium receptor agonist and naloxone.RESULTS Respectively compared with F+NS group,R+NS group or B+NS group,the HPPT of F+N(10 ng)group,R+N(100,10,1 ng)group increased(P<0.05),but the HPPT of B+N(100,10,1 ng)group had no marked change(P>0.05);The stretching numbers of mice of F+N(100,10 ng)group,R+N(100,10,1 ng)group,B+N(10 ng)group decreased(P<0.05 or P<0.01).CONCLUSION Low-dose naloxone can increase the pain-killing effect of opium receptor(partial)agonist.

Objective To observe the effect of naloxone in small dose on the respiratory rate and blood pressure in rabbits under ketamine so as to provide a basis of appropriate combination of clinical medication.Methods Rabbits were given intravenous injection of ketamine 8 mg/kg through auricular veins before they were randomly divided into na-loxone group and saline group.Intravenous injection of naloxone(0.01 μg/kg) was given to observe the effect on the blood pressure and respiratory rate in rabbits by xiphoid dissociation,common carotid catheterization,respectively.Results Naloxone had no significant effect on the blood pressure in rabbits under ketamine(P0.05),but it could antagonize ketamine-induced respiratory rate slow down(P0.01).Conclusion Naloxone in small dose can antagonize ketamine-induced respiratory inhibition.

Objective To observe the analgesic effect of low dose naloxone.Methods One hundred mice were randomly divided into group NS(only with 0.9% sodium chloride) and group N1, group N2, group N3, group N4(with naloxone 1 000, 100, 10, 1 ng/kg respectively).Pain threshold in hot-plate test(HPPT) were observed.Results HPPT of group N1 at 5,10 min and HPPT of group N2 at 5 min increased significantly compared with their basis HPPT(P0.05 and P0.01), but only HPPT of group N1 at 5 min increased significantly compared with group NS(P0.01).Conclusion Low dose naloxone(1～100 ng/kg) have no significant analgesic effect.

Objective To observe the surface anesthetic effect of verapamil hydrochloride combined with lidocaine hydrochloride on rabbits.Methods 12 rabbits were used in the blink reflex experiment to observe the surface anesthetic effect of physiological saline combined with lidocaine hydrochloride(left eye),and verapamil hydrochloriden combined with lidocaine hydrochloride(right eye) on rabbits respectively.Results Blink times of the right eye are fewer than those of the left eye(P<0.01).Conclusion Verapamil hydrochloride can enhance the surface anesthetic effect of lidocaine hydrochloride.

Objective To observe the influence of low dose naloxone(naloxone) on the analgesic effect of fentanyl(fentanyl).Methods Analgesic dose given to the mice were divided into FS Group(fentanyl combined with NS Group), FN1 group, FN2 group and FN3 Group(fentanyl and small dose of naloxone 100、10、1 ng/kg combination group) four groups.With hot plate and writhing on the FN was observed in mice hot plate analgesic pain threshold(pain threshold in hot-plate test, HPPT) and writhing number of implications.Results Compared with the FS, FN2 group, HPPT of group FN2 and FN3 HPPT in 5 min increased(P <0.05), writhing number reduction(P <0.05).Conclusion Low dose naloxone can enhance the analgesic effect of fentanyl.

目的 观察小剂量纳洛酮对瑞芬太尼镇痛效应的影响.方法 将给予镇痛剂量瑞芬太尼的小鼠分为RS组(瑞芬太尼与生理盐水合用组)以及RN1组、RN2组和RN3组(瑞芬太尼与小剂量纳洛酮100 ng/kg、10 ng/kg、1 ng/kg合用组)4组.用热板法和扭体法观察纳洛酮对瑞芬太尼镇痛小鼠热板法痛阈(HPPT)和扭体次数的影响.结果 与RS组比较,RN1组、RN2组和RN3组的HPPT在5 min、10 min、20 min显著增高(P＜0.05),扭体次数显著减少(P＜0.05或P＜0.01).结论 1～100 ng/kg的纳洛酮能够增强瑞芬太尼的镇痛效应.