苯丙氨酸代谢途径是植物最重要的次生代谢途径之一,其下游产物包括异黄酮、植保素、木质素等多种次生代谢产物.苯丙氨酸解氨酶(PAL)是苯丙氨酸代谢途径的起始酶和关键酶.PAL基因家族包含8个基因,为了明晰这8个基因在大豆植株内的表达情况,通过实时定量PCP研究了PAL家族所有成员在大豆植株内的时空表达差异性.发现PAL基因家族总体的相对表达量在生殖生长时期高于营养生长时期,在叶片和子粒中相对表达量较高.PAL1-1、PAL2-1和PAL2-3基因在各部位相对表达量明显高于其他同家族基因,PAL1-1基因各时期稳定表达,PAL2-1和PAL2-3基因在生殖生长后期先后出现表达峰值.这些结果表明大豆PAL基因家族各成员的相对表达量在时间和空间上存在差异,具有一定时空表达特异性.

This paper studied effects of medium composition,tool enzyme system,washing solution and number of times of mix when added the plasmid on Aspergillus niger protoplast transformation frequency.The result showed that the frequency obtained was 500 transformants per 10 μg plasmid after optimizing condition.

construction of mutant library are a topic and attractive theme in functional genome project.Insertional mutagenesis has the advantage that the inserted element acts as a tag for gene identification.Insertional mutagenesis using T-DNA,transposons and plasmids are commonly methods to creating a mutant library.here we summarize these strategies as well as the progress in the functional genomes research.