苯丙氨酸代谢途径是植物最重要的次生代谢途径之一,其下游产物包括异黄酮、植保素、木质素等多种次生代谢产物.苯丙氨酸解氨酶(PAL)是苯丙氨酸代谢途径的起始酶和关键酶.PAL基因家族包含8个基因,为了明晰这8个基因在大豆植株内的表达情况,通过实时定量PCP研究了PAL家族所有成员在大豆植株内的时空表达差异性.发现PAL基因家族总体的相对表达量在生殖生长时期高于营养生长时期,在叶片和子粒中相对表达量较高.PAL1-1、PAL2-1和PAL2-3基因在各部位相对表达量明显高于其他同家族基因,PAL1-1基因各时期稳定表达,PAL2-1和PAL2-3基因在生殖生长后期先后出现表达峰值.这些结果表明大豆PAL基因家族各成员的相对表达量在时间和空间上存在差异,具有一定时空表达特异性.

Phytophthora root and stem rot of soybean(Phytophthora sojae)has become a serious damage to soybean production in China,especially in main soybean production region,Heilongjiang province.Polyphenol oxidase(PPO)is an important enzyme related to disease resistance in plants.However,there are few reports about response of PPO activity in wild soybeans infected with Phytophthora sojae.A total of eight wild soybeans including four resistant and four susceptible ones were inoculated with zoospores of P.sojae race 1 as treatment and those inoculated with water as control.The results showed that PPO activity in roots and leaves of resistant wild soybeans increased compared with those of control at the whole stage of pathogenetic process,and that in stems also increased at most stages of pathogenetic process.However,the PPO activity in roots and leaves of the four susceptible wild soybeans decreased at most stages of pathogenetic process.The results could lay theoretical basis on physiological and biochemical mechanism for disclosing wild soybean's resistance to P.sojae.

As a positive functional factor for human health,lutein has great prospective application in development values in health food area.In recent years,lutein is a hot topic in the research of functional food.The recently progresses in the structures and function,detection methods,synthetic route and key genes,inherited character and influence factors of soybean lutein were reviewed in this article.

The resistance of soybean cultivars to soybean cyst nematode(SCN) closely related with some agronomic traits.Therefore,studying the relationship between characters of SCN resistance and agronomic characters had great reference value for breeding practice.A recombinant inbred lines(RILs) population with 141 F2:8 individuals derived from the cross of L-10,a nonrace specific soybean line as male parent,and Heinong 37 as female parent,was taken as materials.Plastic bag method was introduced in greenhouse disease nursery to evaluate the resistance of soybean RILs,and correlation analysis between SCN resistance and main agronomic characters was conducted.Results showed the cysts parasitic index exhibited a negative correlation with plant height,pods number per plant and 100-seed weight;the cysts parasitic index had a correlation with flower color,and had a significant correlation with seed coat and hilum color.The high-resistance varieties exhibited the character of oval grains,black seed coat,black hilum and brown pubescence,and these qualitative traits can provide referable basis for selection of soybean varieties with high resistance to cyst nematode.