This study aimed to analyze the changes associated with serum miRNAs in patients with cervical intraepithelial neoplasia (CIN) and cervical cancer in predicting early-stage cervical cancer. A total of 229 serum samples were collected to extract miRNAs. The expression level of miR-146b-5p, miR-192-5p and miR-122-5p were determined via qRT-PCR (Quantitative Real-time PCR), and their relative expressions were calculated using the equation: 2-triangle triangle Ct. We observed significantly higher expression levels of serum miR-146b-5p, miR-192-5p and miR-122-5p in the cervical diseases (CIN and cervical cancer) compared with controls. The expression levels of serum miR-146b5p, miR-192-5p and miR-122-5p in cervical cancer were significantly higher than in controls. The relative expressions of serum miR-146b-5p and miR-192-5p in CIN were significantly higher than controls. However, there was no difference in three miRNAs between CIN and cervical cancer. The relative expressions of serum miR-146b-5p in patients classified as FIGO (International Federation of Gynaecology and Obstetrics) stage I were significantly higher than in controls and CIN. The AUC (area under the ROC curve) of the three miRNAs combined with SCC (squamous cell carcinoma antigen) in diagnosing cervical cancer was 0.840, and the sensitivity and specificity were 0.632 and 0.957, respectively. Serum miR-146b-5p, miR-192-5p and miR-122-5p showed promising efficacy as noninvasive biomarkers to accurately diagnose cervical cancer and demonstrated certain early diagnostic values, especially miR-146b-5p and miR-1925p. The combined use of the three serum miRNAs with SCC could further improve the diagnostic efficiency for cervical cancer.

BackgroundDermatomyositis‐associated interstitial lung disease (DM‐ILD) represents a severe and insidious complication of dermatomyositis (DM). The study aimed to investigate the association between DM‐ILD and arterial blood gas indices, serum ion levels, and the timing of interstitial lung disease onset, with the goal of identifying potential predictors for DM‐ILD.MethodsThe investigation involved the collection of basic data from 89 patients with DM hospitalized at the Chinese PLA General Hospital between January 2019 and April 2022, and 43 normal control patients hospitalized for physical examinations during the same period. Analyses were conducted to explore the relationship between DM‐ILD, arterial blood gas indices, disease duration, and serum ions. A regression model to predict DM‐ILD was developed using these indices, and a receiver operating characteristic curve was generated.ResultsSignificant differences were observed in pH and PaO2 between the control group and the disease group (p < 0.05). The DM group exhibited higher levels of pH, actual bicarbonate, and base excess (BE) compared with the control group. In contrast, pH and BE levels were lower in the DM‐ILD group than in the DM group, with these differences being statistically significant (p < 0.05). Interstitial lung disease was correlated with the duration of the disease and pH levels (p < 0.05). The cutoff values for age, disease duration, pH, and Cl− were 55.5 years, 5.5 years, 7.432, and 101.5 mmol/L, respectively. The model demonstrated a prediction sensitivity and specificity for DM‐ILD of 0.809 and 0.722, respectively, with an area under the curve of 0.809.ConclusionArterial blood gas analysis and serum Cl− levels may assist in predicting DM‐ILD. A combined monitoring approach involving arterial blood gas pH, disease duration, age, and serum Cl− levels could enhance the accuracy of DM‐ILD predictions and hold significant clinical evaluation potential.

目的 探讨血常规、生化、血清肿瘤标志物对皮肌炎的临床辅助诊断价值.方法 收集皮肌炎患者90例(疾病组),并收集同期85名健康体检者(对照组)的临床信息,分析患者及健康体检者的基本信息,对相关血液学指标的水平差异进行分析,并通过二元Logistic回归比较单一指标和联合诊断的差异,做出相应的ROC曲线.结果 疾病组细胞角质素片段19(CYFRA211)、神经元特异性烯醇化酶(NSE)、白细胞计数(WBC)水平较对照组显著升高(P<0.001).CYFRA211、NSE和WBC单独诊断皮肌炎时,ROC曲线下面积AUC均小于三者联合检测时的AUC,三者联合检测约登指数为0.729,敏感度和特异度分别为0.787、0.942,曲线下面积AUC为0.923.结论 CYFRA211、NSE及WBC联合检测对皮肌炎具有一定的临床辅助诊断价值.

PURPOSE To investigate serum leptin levels in patients with type 2 diabetes mellitus (T2DM) and the relationship between leptin levels and T2DM complications and prevalence. METHODS A total of 355 patients, 282 cases with T2DM and 73 normal controls, were recruited at 1st Medical Centre, Chinese PLA General Hospital (Beijing, China) between November 2013 and July 2014. Levels of serum leptin, biochemical markers and sexual hormones were measured, and clinical characteristics were retrieved through the electronic medical record system. RESULTS Leptin levels in females were higher than that in males. Leptin levels in T2MD patients were positively correlated with body mass index, percent body fat, triglyceride, cystatin C homocysteine and salivary acid, and negatively correlated with glycosylated serum protein and glycosylated albumin levels. Leptin levels in males were positively correlated with systolic pressure and estradiol, and negatively correlated with testosterone and high density lipoprotein cholesterol. Sex (female) was positively correlated with the duration of disease. Leptin levels in T2DM patients with complications such as hypertension, diabetic nephropathy, diabetic peripheral neuropathy and coronary heart disease were higher than that in patients without such complications. Leptin levels in females with diabetic retinopathy and diabetic macroangiopathy were higher than that in patients without such complications, but there was no difference in males. CONCLUSIONS Leptin has significant gender differences. Leptin levels are related to body mass index, percent body fat and sex hormone level in T2DM patients and may affect short-term blood glucose control in T2DM patients. Leptin levels are related to complications in patients with T2DM and affect the prevalence rates of complications.

BACKGROUND The reference intervals of biochemical markers were significantly affected by age and gender, especially in minors. In recent years, many provinces and regions in China had established reference intervals for children's hematological indicators. Without considering the instruments and reagents, the reference interval will also be affected by the region, economic development, eating habits, and other factors. Therefore, the reference interval of any hematological indicators is not necessarily a fixed range, it changes with certain factors. In our study, we analyzed the changes of biochemical markers in different serum total Ca and 25-OH-D concentrations, and established the reference intervals of biochemical markers in 3-year-old children, explored the change trend of biochemical markers with different serum total Ca and 25-OH-D concentration. METHODS Data was collected from 226 cases of 3-year-old children for biochemical markers, in the Chinese PLA General Hospital in August 2015. The data were divided into a high-level group (serum total Ca > 2.63 mmol/L and 25-OH-D > 40.81 ng/mL) and a low-level group (serum total Ca < 2.54 mmol/L and 25-OH-D < 32.64 ng/mL) according to serum total Ca and 25-OH-D levels for comparison. The change trend of biochemical markers was compared according to serum total Ca and 25-OH-D level. RESULTS The Glu levels in boys were significantly higher than that in girls, but CHO and LDL-C in girls were significantly higher than that in boys. The reference intervals of ATL (5.6 - 22.1 U/L), ALB (44.8 - 55.2 g/L), TP (62.7 - 83.1 g/L), ALP (154.4 - 379.7 U/L), GGT (7.2 - 15.9 U/L), Glu (boys: 4.08 - 5.91 mmol/L; girls: 4.05 - 5.37 mmol/L), UREA (2.7 - 6.3 mmol/L), CREA (26.4 - 46.8 µmol/L), UA (182.4 - 400.2 µmol/L), TG (0.43 - 1.67 mmol/L), CHO (boys: 3.19 - 5.96 mmol/L; girls: 3.03 - 6.51 mmol/L), HDL-C (0.98 - 2.24 mmol/L), LDL-C (boys: 1.30 - 3.64 mmol/L; girls: 1.24 - 4.27 mmol/L), total Ca (2.34 - 2.85 mmol/L), PHOS (1.38 - 2.06 mmol/L), Mg (0.83 - 1.06 mmol/L), osteocalcin (41.64 - 91.92 ng/mL), PTH (12.08 - 43.06 pg/mL), 25-OH-D (19.66 - 56.37 ng/mL), β-CrossLaps (0.82 - 1.88 ng/mL), TP1NP (357.9 - 1025.7 μg/L) were established. ALT, TP, ALB, GGT, Glu, CHO, HDL-C, LDL-C, UREA, CREA, PHOS, Mg, and ALP in high level group were significantly higher than those in low level group. There was no significant difference in TG, UA, TP1NP, osteocalcin, PTH and β-CrossLaps between high level group and low-level group. With the increase of serum total Ca and 25-OH-D levels, most of the biochemical markers had a gradually increasing trend. However, biochemical markers of bone (TP1NP, osteocalcin, PTH, β-CrossLaps) showed different trends. CONCLUSIONS This study established the reference intervals of biochemical markers of 3-year-old children. The changes of serum total Ca and 25-OH-D levels in children reflected the changes of glucose and lipid metabolism, liver and kidney function markers, and indirectly reflected the growth and development of children and various organ functions. Maintaining high levels of serum total Ca and 25-OH-D can promote the growth and development of children.

目的 建立3岁儿童血清铁(IRON)、不饱和铁结合力(unsaturated iron binding capacity,UIBC)、总铁结合力(total iron binding capacity,TIBC)、叶酸(Folate)、维生素B12(vitamin B12,VB12)参考区间.方法 收集2015年8月我院进行入幼儿园查体的226例3周岁儿童血清IRON、UIBC、TIBC、Folate和VB12检测结果 ,并建立参考区间.结果 经离群值检测血清IRON缺失5例,剔除1例样本;UIBC缺失5例,剔除1例样本;TIBC缺失5例,剔除1例样本;Folate缺失1例,无剔除样本;VB12无缺失和剔除样本;男童与女童UIBC(P=0.029)与TIBC(P=0.015)差异具有统计学意义(P<0.05),血清IRON、Folate和VB12等3项男童与女童之间差异无统计学意义(P>0.05);相关性分析结果 显示,血清IRON与UIBC呈负相关(r=-0.532,P<0.001);Folate与VB12呈正相关(r=0.373,P<0.001);UIBC与TIBC呈正相关(r=0.783,P<0.001).3岁儿童参考区间分别为IRON(6.4～29.9μmol/L)、UBIC(男童:30.9～67.6μmol/L;女童:29.3～65.2μmol/L)、TIBC(男童:51.5～81.1μmol/L;女童:50.0～81.3μmol/L)、Folate(6.6～20.0 ng/mL)和VB12(306.6～1721.4 pg/mL).结论 建立3岁儿童血清IRON、UIBC、TIBC、Folate和VB12参考区间对正确评估儿童发育、疾病诊断和治疗效果具有重要临床意义.

目的 探讨生命体征评估法在慢性阻塞性肺疾病(COPD)急性加重期患者病情评估中的应用价值,以期为科学、量化进行急诊分诊提供依据.方法 回顾性研究,采用便利抽样连续入组的方法,收集2019年1月1日-12月31日在北京市某三甲综合医院急诊科诊断为慢性阻塞性肺疾病急性加重期的152例患者,病情平稳组共121例(79.61%),病情危重组31例(20.39%).收集其性别、年龄、COPD确诊年限等基本信息、利用生命体征法对病情严重程度进行评估,并追踪其转归.利用卡方检验、非参数检验对病情危重与病情平稳两组患者基本资料进行单因素分析,生命体征评估法对患者的评价与患者结局是否一致采用Cohen,s kappa系数分析.结果 应用生命体征评估法可正确判断20例患者为病情危重患者,占全部危重患者的64.52%,对2组患者生命体征的具体项目进行比较,其中意识状态、呼吸、脉搏的差异具有统计学意义(P<0.05),正确判断117例患者为病情平稳患者,占全部病情平稳患者的96.69%,kappa值为0.668,具有较强的一致性.NEWS评分法对患者评估结果与实际转归的kappa值0.454,具有中等强度的一致性.结论 生命体征评估法不仅使用起来便利、快捷,无需浪费更多宝贵的时间进行相对较为繁琐的计算,且其对患者转归的预测效能更胜一筹.生命体征评估法可以作为一种简单的工具,用于急诊分诊人员对慢性阻塞性肺疾病急性加重期患者的病情严重程度的快速评估.

目的:本研究拟分析血清硫氧还蛋白相互作用蛋白(thioredoxin interacting protein,TXNIP)在2型糖尿病(T2DM)患者血清中的变化规律.方法:收集2013年11月至2014年7月在解放军总医院内分泌科住院、明确诊断为2型糖尿病患者(270例)与同时期健康体检者(73例)血清,分别检测血清TXNIP(ELISA法)、血清葡萄糖(Glu)、糖化血红蛋白(HbA1c)等指标.结果:①2型糖尿病慢性并发症患病率为视网膜病变20.5％、糖尿病肾病20.1％、神经病变34.3％、高血压50.4％、冠心病12.6％.②血清TXNIP水平在年龄与并发症分组中差异具有统计学意义(P＜0.05).在年龄分组中,年龄＜45岁组血清TXNIP水平显著高于年龄45 ～ 60岁组(P =0.026),年龄＞60岁组血清TXNIP水平显著高于年龄45 ～ 60岁组(P=0.021),其余无统计学差异.在并发症分组中,D组TXNIP水平显著高于A组(P =0.025),C组TXNIP 水平显著高于E组(P =0.025),D组TXNIP水平显著高于E组(P=0.002),D组TXNIP水平显著高于F组(P =0.045),G组TXNIP水平显著高于E组(P =0.028).③血清TXNIP水平随Glu与HbA1c分层变化趋势呈波动状态.④相关性分析显示,血清TXNIP与身体质量指数(BMI)呈正相关(r=0.160,P=0.009),与血小板(PLT)呈负相关(r=-0.125,P=0.041).结论:血清TXNIP水平改变与2型糖尿病慢性并发症密切相关,在2型糖尿病及其血管并发症中起着重要的作用.控制血清TXNIP水平可能有助于控制2型糖尿病患者血管并发症的发生与发展.

目的 探讨血清硫氧还蛋白相互作用蛋白(Thioredoxin interacting protein,TXNIP)诊断与其他血液学指标联合诊断2型糖尿病的价值及临床意义.方法 回顾性分析2型糖尿病患者(270例)与同时期健康体检者(73例)血清TXNIP水平(ELISA法)、一般临床资料和相关血液学指标.结果 2型糖尿病组血清TXNIP与唾液酸(SA)水平显著高于健康对照组(P<0.01).ROC曲线分析结果显示:血清TXNIP与SA单独诊断2型糖尿病的线下面积AUC分别为0.606(敏感度0.815,特异性0.438,约登指数0.253,P=0.003)与0.646(敏感度0.632,特异性0.616,约登指数0.248,P=0.000).TXNIP联合SA、高密度脂蛋白胆固醇(HDL-C)、收缩压等联合诊断2型糖尿病的线下面积AUC=0.749(敏感度0.494,特异性0.903,约登指数0.397),差异具有统计学意义(P=0.000).结论 血清TXNIP检测可对2型糖尿病诊断提供一定的参考价值,同时为预防和治疗2型糖尿病的分子生物学靶点提供了方向.

Objective To analyze the differences of biochemical and immunological indicators in gender or Lee′s classification of IgA nephropathy ( IgAN) to provide laboratory evidence for clinical diagnosis and treatment of IgAN.Methods Retrospective cohort study.The information of biochemical and immunological indicators of 213 in-hospital patients which were admitted in Chinese PLA General Hospital in June to December, 2012.The data were collected and analyzed with t-test, non-parameter analysis, Pearson correlation analysis, ROC curves analysis and Logistic regression analysis according to gender or Lee′s classification ( Lee′s≤3 group and Lee′s>3 group).Results In this study, the average age and sex ratio of patients with IgAN was ( 35.0 ±10.6 ) years old and 2.04∶1.00.T-test and non-parameter analysis indicated homocysteine(HCY), immunoglobulin E (IgE), immunoglobulin M (IgM), prealbumin (PA), ceruloplasmin ( CP) ,α1-acidoglycoprotein(α1-AGP) , albumin ( Alb) ,α2-globulin(α2-G) andγ-globulin (γ-G) had significant difference in gender (P<0.05).And cystantin-C (CYS-C), HCY, complement 4 (C4),β2-microglobulin(β2-MG), PA, α1-AGP, α1-globulin(α1-G), immunoglobulin G (IgG), IgM and transferrin ( TF ) had significantly different in Lee′s classification ( P<0.05 ).ROC curves analysis showed that the areas under curves of CYS-C andβ2-MG were 0.891 and 0.839 respectively;the sensitivity was 90.2%and specificity was 75.3% when cutoff value of CYS-C was 1.56 mg/L; the sensitivity was 80.3%and specificity was 78.8% when cutoff value of β2-MG was 0.275 mg/dl.Logistic regression analysis showed that CYS-C ( OR: 31.380, 95% CI: 10.808 -91.113, P=0.000 ) and HCY ( OR:1.035, 95%CI:1.002-1.069, P=0.040) were introduced into the model after twice variable selections.ROC curve of CYS-C combined with HCY for classifying Lee′s classification showed that the sensitivity, specificity, Jorden index and AUC were 0.843, 0.862, 0.705 and 0.894 ( P=0.000 ) , respectively.Conclusions CYS-C,β2-MG, PA and HCY were valuable indicators for Lee′s classification.CYS-C andβ2-MG had high correlation with Lee′s classification, which indicated that these two tests were related to the severity of IgAN.CYS-C combined with HCY could improve the diagnostic efficiency of IgAN Lee′s classification.Combination of biochemical and immunological indicators could improve the accuracy of Lee′s classification and provide effective laboratory evidence for clinical treatment of IgAN.

目的 探讨血清CA125(糖类抗原125)与BNP(B型脑利钠肽)水平在心力衰竭患者心功能分级中的变化及其临床意义.方法 收集118例我院2011年1月至2011年12月住院且诊断为心力衰竭患者数据进行回顾性统计学分析.结果 与健康对照组比较,NYHA Ⅱ(纽约心脏病协会心功能分级)、NYHA Ⅲ、NYHA ⅣV、心血管疾病无合并心力衰竭组四组血清CA125、BNP水平均显著高于健康对照组(P＜0.01),且血清CA125与BNP水平升高与NYHA分级呈正相关.不同病因(冠心病、风湿性心脏病、高血压性心脏病、扩张型心肌病、心脏瓣膜疾病)组血清CA125、BNP水平两两比较扩张型心肌病组血清CA125水平显著高于高血压性心脏病组(P＜0.05),而BNP水平在不同病因组中无统计学差异.左心室扩大组血清CA125水平显著高于正常左心室组(P＜0.01);而LVEF水平显著低于正常左心室组(P＜0.01).NYHA分级有胸腔积液、心包积液、房颤组血清CA125水平均高于无上述体征组,且差异具有统计学意义(P＜0.01).血清CA125与BNP呈显著正相关(P＜0.01);血清CA125与LVEF(左室射血分数)呈显著负相关(P＜0.01).结论 结合血清CA125、BNP、LVEF等指标可判断HF严重程度,并为NYHA分级提供实验室辅助依据.血清CA125、BNP、LVEF等指标作为临床实验室指标,综合病因、症状、体征和其他非创伤性指标可有效监控HF的发生与发展.

Objective To investigate the genotype and age distribution of high risk papilloma virus(HPV) infection in female.Methods The HPV-DNA genotypes HPV-DNA 16,HPV-DNA 18/45,HPV-DNA 31 and HPV-DNA 33/52/58/67 in 6319 cases were detected by dual-channel real time fluorescence quantitative PCR,and the high risk HPV-DNA genotypes of positive rate and distribution in different age groups were analyzed.Results 794 cases positive samples were detected in 6319 female with a positive rate of 12.6%.The age 51-60 group and ≥61age group had same positive rate of 15.8% and significantly higher than that of the other groups(P0.05).The age 21-30 group had the secondly highest positive rate(13.8%).Different high risk genotypes of positive rate from high to low was HPV-DNA 33/52/58/67(69.3%),HPV-DNA 16(21.5%),HPV-DNA 18/45(12.1%) and HPV-DNA 31(6.9%).Conclusion High risk HPV-DNA testing is an effective screening method for cervical cancer.It should be a project of required to choose in health examination for female in the age from 38.2-57.6,and it has great significance in diagnosis and treatment of cervical cancer.

To explore the clinical significance of serum ferritin level assay for cardio vascular and cerebrovascular disease and metabolic disease,the levels of serum ferritin and biochemical indicators in 82 cases of cardiovascular and cerebrovascular disease,60 cases of metabolic disease and 111 healthy subjects were detected with the ROCHE E170 and HITACHI 7600 automatic biochemical analyzer,and the results were analyzed by SPSS 17.0.The results showed that the serum ferritin levels in cardiovascular and cerebrovascular disease group were significantly higher than that of metabolic disease group and healthy control group(P0.01),and the levels of serum ferritin in metabolic disease group were significantly higher than that of healthy control group(P0.05).The levels of serum ferritin in cardiovascular and cerebrovascular disease group were significantly correlation with BUN,Apo AI,CK,Cys-C and SA(P0.01);The serum ferritin in metabolic disease group was significantly correlation with Apo AI(P0.01).The serum ferritin levels in cardiovascular and cerebrovascular disease and metabolic disease were increased in different levels,and the serum ferritin has an important clinical significance in diagnosis of cardiovascular and cerebrovascular disease and metabolic disease.