Objective To explore the expression of HIF-2α and its association with the expression of TAM and VEGF-C in breast cancer. Methods The expressions of HIF-2α,TAMs and VEGF-C were detected by immunohistochemistry in 53 human breast cancer samples and 20 normal breast tissue samples. Results ①The positive rate of HIF-2,TAM,VEGF-C in breast cancer were higher than normal tissue, the difference had statistical significance; ② In 53 cases of breast cancer, 18(34%), 32(60%), 31 (58%)were positive for HIF-2α、TAMs and VEGF-C, respectively; ③ A significant difference was found in terms of expression of HIF-2α between the samples with TAMs either positive or negative (P<0.01), The same difference was found in VEGF-C (P<0.01); ④ Not only the HIF-2α expression but also the expression of VEGF -C in breast cancer was related to the expression of TAMs. Conclusion The positive expression of HIF -2α is strongly correlated with the expression of TAMs and the positive expression of TAMs was also correlated with VEGF-C.