OBJECTIVES:Infection by carbapenem-resistant Pseudomonas aeruginosa (CRPA) is a serious clinical problem worldwide. However, the molecular epidemiology of the clinical isolates varies depending on the region. This study was conducted to analyse the resistance phenotype and clarify the genetic and epidemiological properties of CRPA clinical isolates from southeast Shanxi, China. METHODS:Fifty-seven isolates of CRPA were collected from a hospital in this region. These isolates were reidentified by MALDI-TOF and subjected to whole-genome sequencing by next-generation sequencing. Phylogenetic trees were constructed based on single nucleotide polymorphisms (SNPs), after which multilocus sequence typing (MLST) was performed and antimicrobial resistance genes were identified. RESULTS:All the 57 CRPA isolates carried at least one kind of gene encoding carbapenemase, such as blaIMP-1, blaIMP-10, blaOXA-10, blaOXA-395, blaOXA-396, blaOXA-485, blaOXA-486, blaOXA-488, blaOXA-494, and blaOXA-50. The isolates harboured AIM-1, CMY-51, mecD, and NmcR genes and carried one kind of Pseudomonas-derived cephalosporinase (PDC) β-lactamase-encoding gene, such as blaPCD-1 to blaPCD-3, blaPCD-5, or blaPCD-7 to blaPCD-10. Two isolates were found to harbour the aminoglycoside-modifying enzyme genes aadA1 and aadA7; however, no isolates were found to harbour genes encoding 16S rRNA methylase or quinolone resistance-related genes. These CRPA isolates belonged to various sequence types (STs), two of which, namely, ST235 and ST277, were high-risk types. CONCLUSIONS:Our findings indicate that CRPA isolates carrying resistance genes with unique regional characteristics are spreading in this region, with a high diversity of STs, especially in high-risk clones. These findings highlight the necessity for further measures to prevent CRPA spread in Shanxi.

目的:探讨美洲大蠊提取物(PAS840)对大鼠肾上腺嗜铬细胞瘤(PC12)细胞氧化损伤模型的保护作用及机制.方法:采用H2O2刺激PC12细胞建立神经细胞氧化损伤模型,实验分为正常组(Con)、模型组(Mod)、PAS840低中高剂量组(20,50,125 μg·mL-1 的PAS840培养基溶液进行处理),采用倒置显微镜观察细胞形态并采用CCK-8法检测各组细胞存活率,生化试剂盒检测各组超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、乳酸脱氢酶(LDH)、谷胱甘肽(GSH)和丙二醛(MDA)的水平;DCFH-DA荧光探针检测各组活性氧簇(ROS)的水平;流式细胞术检测各组细胞凋亡率;JC-1法染色检测各组细胞线粒体膜电位(MMP);RT-qPCR检测各组Nrf2/HO-1通路因子(Nrf2、Keap1、HO-1和NQO1)、凋亡因子(Bcl-2、Bax和Caspase-3)、炎症因子(TNF-a、IL-1β和IL-6)、乙酰胆碱酯酶(AchE)和过氧化氢酶(CAT)mRNA的表达水平;Western blot法检测各组Nrf2、HO-1、Bcl-2、Bax和Caspase-3蛋白的表达水平.结果:PAS840可显著提高氧化损伤细胞的存活率、MMP及 SOD、GSH-Px和 GSH 的水平,降低LDH、MDA和 ROS 的水平;显著降低Keap1、TNF-a、IL-1β、IL-6 和 AchE mRNA表达的同时,显著增加CAT和NQO1 mRNA的表达,显著降低Nrf2、Bax和Caspase-3的mRNA及其蛋白表达,显著增加HO-1和Bcl-2的mRNA及其蛋白表达.结论:PAS840可以抑制H2O2诱导的PC12细胞凋亡,减轻炎症,其机制可能与降低ROS、调控Nrf2/HO-1通路因子减轻细胞的氧化损伤程度有关.

Background: Cyclophosphamide (CTX) is widely used in tumor treatment, but its clinical therapeutic effect is not ideal due to many side effects. Materials and Methods: In the present study, we researched the synergistic and attenuating effects of CII-3 combined with CTX and their underlying mechanism in H22 tumor-bearing mice. Firstly, we established an H22 tumor-bearing mice model, and the body weight, tumor weight, and survival time were recorded. Secondly, HE staining of tumor tissue was performed, and the related organ index, NK cell killing activity, peripheral blood cells, and bone marrow nucleated cells were measured. Moreover, the changes in IL-6 and IFN-1 beta in serum were detected by ELISA. Finally, RT-qPCR and Western Blot were performed to detect the expressions of TLR4, TLR9 and NF-kappa B in tumor tissue. Results: The treatment of CII-3 combined with CTX could increase life extension rate of H22 tumor-bearing mice, reduce tumor weight. Additionally, it could inhibit tumor cell proliferation, increase thymus and spleen index, enhance the activity of T cells in spleen, promote the killing activity of NK cells, and had a certain ameliorate effect on the reduction of WBC, Neut, LYM and bone marrow nucleated cells caused by CTX. And the expression of mRNA and the protein of TLR4, TLR9 and NF-kappa B in tumor mass of H22 tumor-bearing mice were down-regulated. Conclusion:There were synergistic and attenuating effects of CTX combined with CII-3 in the treatment of tumor and the effects might be mediated by the TLR4/NF-kappa B and TLR9/NF-kappa B signaling pathways.

CII-3 is the effective part of Periplaneta americana for application in oncotherapy. This study investigated its main chemical components for macrophage polarization regulation activity. Compounds were separated and purified, and their structures were elucidated based on NMR and HR-ESI-MS analyses. After inducing the M1 and M2 phenotype macrophages, CII-3 and testing components were added and co-incubated to evaluate their effects on the relevant markers of macrophages. Then, gradient concentrations of CII-3 and active monomers were further investigated for their effects on M2 macrophages. The effects were detected by RT-PCR, ELISA, flow cytometry, and immunofluorescence. Twelve compounds were identified from CII-3. CII-3 and pericanaside (5) had no obvious effect on M1 macrophages, while they significantly reduced the expression levels of M2 macrophage markers. Specifically, they significantly reduced the levels of TGF-β and IL-10 and the mRNA expression levels of ARG-1 and CD206 in the M2 phenotypes of RAW264.7 and Ana-1 macrophages. The conditioned medium of CII-3 and pericanaside (5) could inhibit the migration capacity of CT26.WT tumor cells. Macrophage M1/M2 polarization is a dynamic equilibrium, and the M2 phenotype, which can promote the growth of tumor cells, is relatively highly expressed in the tumor microenvironment. CII-3 and pericanaside could significantly reduce the phenotype of M2-type macrophages, indicating that the anti-tumor activity of CII-3 could be related to the inhibitory effect on M2 polarization, and pericanaside was one of the active components.

Rheumatoid arthritis (RA) is characterized by synovial hyperplasia and inflammation, and fibroblast-like synoviocytes play an important role in cell over-proliferation and inflammation. Wasp venom is a folk medicine that is widely used to treat RA among the Jingpo minority in China's Yunnan province. However, how does it treat RA have not been reported. The aim of this study was to explore the effects of wasp venom from Vespa mandarinia Smith on the proliferation and apoptosis of MH7A human RA synovial fibroblasts in vitro. MH7A cells were incubated with different concentrations (0, 12.5, 25, 50, and 100 mu g/mL) of wasp venom. Then, the viability of MH7A cells was assessed using a real-time unlabeled cell analyzer (RTCA) and by annexin V-FITC/propidium iodide (PI) double labeling. The expression of caspase-3, B-cell lymphoma-2 (Bcl-2), and Bcl-2-associated X protein (Bax) was determined by qRT-PCR and Western blotting after the wasp venom treatments. Results showed that wasp venom dramatically inhibited the viability of MH7A cells and induced MH7A synovial cell apoptosis in a dose-dependent manner. The expression of caspase-3 and Bax increased and that of Bcl-2 decreased in the wasp venom-treated group compared with that observed in the control group. Regarding mRNA levels consistent with the protein level results. In conclusion, wasp venom may induce MH7A cell apoptosis through caspase signaling pathways.

The non/hypo-response rate of the hepatitis B vaccine among hemodialysis (HD) patients is still high, it is of great significance to explore the influencing factors and their relationships. To study the related factors and their relationships using logistic regression model and Chi-squared Automatic Interaction Detection (CHAID) decision tree model. A randomized controlled trial was conducted between February 2014 and May 2015 in China. HD patients being serologically negative for HBsAg and anti-HBs were randomly assigned to receive three intramuscular injections of the standard dose (20 µg) or high dose (60 µg) of recombinant hepatitis B vaccine at months 0, 1, and 6. Those with anti-HBs concentrations <100 mIU/mL, and ≥100 mIU/mL at month 7 were considered as non/hypo-response and high-level response, respectively. The non/hypo-response was 31.34% (89/284). After adjustment for confounders, logistic analysis showed that males (OR = 2.203, 95%CI: 1.109-4.367) and those with higher dialysis frequency (>4 times per 2 weeks) (OR = 1.918, 95%CI: 1.015-3.626) had a significant risk of non/hypo-response. While the CHAID analysis showed that gender, dose, and dialysis frequency were influencing factors of non/hypo-response, and gender is most important. The interaction between gender and dialysis frequency had the greatest effect on immunization, and followed by the interaction between dialysis frequency and vaccine dose. Taken together, gender, dose and dialysis frequency were influencing factors of hepatitis B vaccine in HD patients.

目的 考察云南松松塔提取物对脂多糖(LPS)诱导大鼠急性肺损伤的保护作用及其可能的作用机制.方法 SD大鼠随机分为6组,阳性组灌胃给予1.5 mg/kg地塞米松,云南松松塔提取物低、中、高剂量组分别灌胃给予50、100、200 mg/kg云南松松塔提取物,正常组、模型组灌胃给予等容量生理盐水,每天1次,连续4d.末次给药后1h,腹腔注射10 mg/kg LPS诱导大鼠急性肺损伤,4h后处死大鼠取各脏器,计算脏器指数,ELISA测定肺泡灌洗液(BALF)中炎症因子、氧化因子水平,RT-PCR测定肺组织TLR4/NF-κB信号通路分子mRNA相对表达.结果 与模型组比较,云南松松塔高剂量组大鼠肺脏炎症因子和氧化因子水平、脾脏指数、肝脏指数和肾脏指数均降低(P＜0.05,P＜0.01),胸腺指数升高(P＜0.01),肺组织肺泡壁和支气管壁炎症细胞数量减少,水肿和充血情况减轻;BALF中炎症细胞因子TNF-α、IL-6、IL-1β、TGF-β水平降低(P＜0.01),IL-10水平升高(P＜0.01),SOD活力增加(P＜0.01),MDA、NO水平降低(P＜0.01);云南松松塔高、中剂量组大鼠肺组织中TLR4、NF-κB、TNF-α、IL-6、IL-1β相对mRNA表达降低(P＜0.01).结论 云南松松塔提取物可能通过抑制TLR4/NF-κB信号通路减轻LPS诱导的大鼠急性肺损伤.

对不同产地牛大力种子的真实度、净度、百粒重、发芽率、发芽势、生活力等方面进行了质量分析,为制定南药牛大力种子质量标准提供依据.

校企合作调研南药产业需求,制订南药特色人才培养方案,搭建"广药集团特色班"合作平台,组建南药教学团队,精准对接工作岗位,融入工匠精神,引入优秀企业文化和多元化评价机制,探索产教融合背景下"广药集团特色班"人才培养模式和做法,为职业院校对特色南药技能人才培养提供新途径、新经验,推动云浮市南药产业的高质量发展.

Cyclophosphamide (CTX) is widely used in the clinical treatment of cancer; however, CTX has some severe adverse effects, especially on the immune system. In previous studies, Periplaneta americana L. extract (PE) has been reported to have antitumor and immunoprotective effects on tumor-bearing mice. However, it is not clear whether PE combined with CTX has synergistic and attenuating effects on tumor-bearing mice. In the present study, we researched the synergistic and attenuating effects of PE combined with CTX and their underlying mechanism in MFC tumor-bearing mice. The results showed that PE combined with CTX could significantly enhance the antitumor effect of CTX, which significantly reduced the tumor weight. In addition, we found that PE combined with CTX could significantly increase the thymus, spleen, liver, lung indexes and the number of nucleated cells in the bone marrow. Histopathologic examination of spleen tissues showed that PE could decrease the toxicity induced by CTX in the spleen. Our data indicate that PE significantly decreased the toxicity of CTX in MFC tumor-bearing mice. RT-PCR analysis revealed decreased levels of TAK1 and NF-kappa B mRNA in tumor tissue, suggesting that these effects may be mediated by the TAK1/NF-kappa B signaling pathway.

In order to explore the effects of Xinmailong (XML) injection on cerebrovascular diseases (CVDs), the contents of prostaglandin E-2 (PGE(2)) and related enzyme of mouse brain microvascular endothelial cells (BMECs) were detected. To explore the effect of XML on the Growth of bEnd.3 cells (mouse brain microvascular endothelial cells) by MTT, the contents of PGE(2) and related enzymes were determined by ELISA, RT-qPCR and Western blot analysis. MTT assay showed that the OD value increased in all groups except the XML 12.5 mu g/mL and 50 mu g/mL groups at 24 h incubation time (p < 0.01 or P < 0.05). The ELISA results showed that addition of different doses of XML, PGE(2), cyclooxygenase-1(COX-1) and cyclooxygenase-2(COX-2) gradually decreased (p < 0.01 or p < 0.05). RT-qPCR and Western blot analyses showed that the mRNA and protein levels of 15-PGDH were up-regulated as the dose increased and the expression of COX-2 was down-regulated (p < 0.01 or p < 0.05). XML injection has an inhibitory effect on the levels of PGE(2) in bEnd.3 cells and may regulate the content of PEG(2)-related enzymes to prevent and treat CVDs.

Background: Although the efficacy of hepatitis B vaccines among hemodialysis patients has been documented, the long-term persistence of immunogenicity in this population remains largely unknown. We explored the long-term persistence of immunogenicity induced by different hepatitis B vaccine regimens in hemodialysis patients. Methods: In initial study, we conducted a randomized, multicenter, double-blind, parallel-controlled trial among hemodialysis patients in 13 hospitals in Shanxi Province, China. A total of 352 hemodialysis patients were allocated to receive 3-dose 20 mu g (IM20 group) and 3-dose 60 lg (IM60 group) recombinant hepatitis B vaccine at months 0, 1, and 6. Vaccine-induced immune responses were measured at month 7. In this study, the responders (anti-HBs >= 10 mIU/mL) were followed up at months 18, 24, 30, 36 and 42, respectively. We used the generalized log-rank test and generalized estimating equations (GEE) to analyze the long-term durability of responses and the kinetics of anti-HBs levels, respectively. Results: A total of 284 patients were involved in the extended follow-up period. The duration of vaccine induced response with 75% of patients maintained protective antibody were 12 months and 18 months in the IM20 group and IM60 group, respectively (P = 0.291). The long-term persistent immunogenicity induced by 3-dose 60 lg was more satisfactory than that by 3-dose 20 lg hepatitis B vaccine in patients with hemodialysis duration >= five years (P = 0.023). The peak anti-HBs levels in 100-1000 mIU/mL or >= 1000 mIU/mL were more likely to maintain long-term protective antibody compared to anti-HBs levels in 10-100 mIU/mL (P < 0.05). The kinetic profile was similar between the two groups (P = 0.334). Conclusion: High-dose 60 mu g hepatitis B vaccine could lead a satisfactory long-term durability of immunogenicity among patients with hemodialysis duration of five years or more. Peak anti-HBs level after vaccination was associated with the long-term persistence of immunogenicity. (C) 2021 Elsevier Ltd. All rights reserved.

以葡萄糖为对照品,采用苯酚-硫酸法测定云南松松塔抗肿瘤活性部位的多糖含量.首先考察各显色条件对云南松松塔抗肿瘤活性部位多糖含量测定的影响,确定最佳显色条件为:5％苯酚用量为0.6 mL,水浴温度为60℃,水浴加热时间为20 min,浓硫酸用量为6.0 mL.该方法操作简便、稳定性好,可用于云南松松塔抗肿瘤活性部位多糖含量的测定.

目的 评价不同干燥方法对布渣叶中牡荆苷含量的影响.方法 分别采用阴干、晒干、不同温度热风烘干等方法对布渣叶进行干燥处理,以牡荆苷为指标,通过高效液相色谱法测定干燥后布渣叶中牡荆苷的含量变化情况.结果 采用60℃热风烘干1h,布渣叶中牡荆苷含量最高,为0.058％,70℃热风烘干0.8h牡荆苷含量为0.052％,50℃热风烘干1.2h牡荆苷含量为0.045％,40℃热风烘干1.5h牡荆苷含量为0.043％,阴干荆苷含量为0.043％,晒干荆苷含量为0.042％.结论 不同干燥方法对布渣叶中牡荆苷含量变化有影响,布渣叶经60℃热风烘干1h,其牡荆苷含量高于传统阴干、晒干方法,外观性状优于传统干燥品,是适宜的干燥方法.

目的 考察云南松松塔醇提物对肺纤维化模型小鼠的改善作用.方法 采用气管内一次性注射博来霉素制备肺纤维化模型,造模后第2天开始给予相应药物.实验第28天,眼球取血测定血细胞含量;取胸腺、脾脏和肺,计算免疫器官指数和肺脏指数;肺组织HE染色和Masson染色评价小鼠肺泡炎和肺纤维化情况,免疫组化测定肺组织中STAT3蛋白表达,ELISA法测定血清中IFN-γ、TNF-α、HYP水平和肺组织匀浆中TGF-β含量.结果 与模型组比较,松塔高剂量组小鼠血清TNF-α含量和肺组织匀浆TGF-β含量明显降低(P＜0.05或P＜0.01),上皮细胞中STAT3蛋白阳性表达颗粒明显减少,平均光密度降低(P＜0.01).松塔高、中剂量组小鼠血清中IFN-γ含量明显提高(P＜0.01或P＜0.05),松塔各剂量组HYP含量显著降低(P＜0.01).组织病理学结果显示,与模型组比较,松塔高剂量组肺泡炎及肺纤维化评分显著降低(P＜0.01).结论 云南松松塔醇提物对博来霉素诱导的肺纤维化小鼠具有改善作用,这种作用可能与减轻胶原沉积和减轻炎症反应有关.

Objective: The objective was to study the synergistic and attenuating effects of CII-3 combined with cisplatin. Materials and Methods: A Lewis tumor-bearing mouse model was established. After 15 days of continuous administration of CII-3 and cisplatin, the pathological changes in the tumor, liver, lung, and femur tissues were observed; the life prolongation rate, tumor inhibition rate, Q value, organ indices, spleen T- and B-lymphocyte proliferation activities, NK cell killing activity, the bone marrow cell proliferation rate and cell cycle phase, and the number of peripheral blood cells and bone marrow nucleated cells were measured. The expression of granulocyte colony-stimulating factor (G-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) in mouse serum and bone marrow tissue was measured. Results: The combination of CII-3 and cisplatin could enhance the ability of cisplatin to inhibit tumor cell proliferation and protects the liver damage and femoral injury and could significantly increase the life prolongation rate; tumor inhibition rate of cisplatin; the liver, spleen, lung, and thymus indices; the T- and B-lymphocyte proliferation activity; the NK cell killing activity; and the number of peripheral blood cells and bone marrow nucleated cells. The combination of drugs can stimulate the transformation of bone marrow cells from S phase to G2/M phase, significantly increase the proliferation rate of bone marrow cells and the contents of G-CSF and GM-CSF in mouse serum, and downregulate the mRNA and protein levels of them in bone marrow tissue. Conclusion: These results suggest that CII-3 combined with cisplatin can significantly enhance the antitumor effect and reduce the toxicity and side effects of cisplatin in Lewis tumor-bearing mice.

目的:研究美洲大蠊提取物CⅡ-3与其体外抗肝癌活性的谱效关系,并初步明确其抗肝癌活性成分.方法:在已建立10批CⅡ-3样品的超高效液相色谱(UHPLC)指纹图谱的基础上,借助超高效液相色谱-四极杆-飞行时间质谱联用法(UHPLC-Q-TOF/MS),通过标准品和相关文献定性鉴定各色谱峰对应的化合物;以各批CⅡ-3样品对人肝癌细胞HepG2的半数抑制浓度(IC50)为抗肝癌活性指标,采用灰色关联度分析法(GRA)与正交偏最小二乘法(OPLS)建立并分析指纹图谱与抗肝癌活性之间的谱效关系.结果:10批CⅡ-3样品的UHPLC指纹谱图中有共有峰25个,指认出其中10个,分别为环(酪氨酸-脯氨酸)(峰24)、环(甘氨酸-苯丙氨酸)(峰15)、次黄嘌呤(峰3)、腺嘌呤(峰7)、苯丙氨酸(峰10)、肌苷(峰11)、N-乙酰多巴胺(峰16)、环(脯氨酸-丙氨酸)(峰13)、2-羟基马尿酸(峰22)、环(脯氨酸-丝氨酸)(峰6).10批CⅡ-3样品对HepG2细胞的IC50为70.550～200.303μg/mL.25个共有峰中,以抗肝癌活性的GRA分析关联度(r)排序为峰20>23>24>15,且其r值均大于0.7;以OPLS分析变量重要性投影(VIP)值排序为峰23>18>15>24>7>14>6>2>20,且VIP值均大于1,其中峰7、15、20、23、24的标准回归系数均大于0,峰2、6、14、18的标准回归系数均小于0.联合分析显示,CⅡ-3样品抗肝癌活性主要成分依次为峰20>23>24>15.结论:CⅡ-3中两个未知化合物(峰20、23)和环(酪氨酸-脯氨酸)(峰24)、环(甘氨酸-苯丙氨酸)(峰15)可能是其抗肝癌活性的主要成分.

目的:为解决传统制备炭药存在“太过”和“不及”等问题,规范炭药炮制工艺,提高炭药质量,保证炭药临床用药安全有效.方法:在遵循古法炮制的基础上,结合《中国药典》2015年版炮制通则及《广东省炮制规范》,运用烘法制备炭药.结果:当归片的制备工艺是250℃烘8min,黄芩片250℃烘10min,黄莲片250℃烘10min,槐角段250℃烘10min,荆芥段280℃烘5min,荷叶丝250℃烘8min,丹皮丝250℃烘5min,卷柏段300℃烘3min,艾叶丝300℃烘3min,地榆片300℃烘10min,熟地片300℃烘6min.结论:优选出的制备炭药炮制工艺合理.

观察云南松松塔乙醇提取物(PEA)、碱水提取醇沉物(PED)对H22肝癌小鼠的影响.将H22腹水瘤模型小鼠随机分组,灌胃给药,1次/d,治疗10 d.观察各小鼠一般状况及20 d内的死亡情况,计算生命延长率.建立H22实体瘤模型,分组及给药同前.给药第1天开始,隔天测量1次各小鼠肿瘤直径(mm),计算肿瘤体积;末次给药24 h后,取脾脏观察T细胞和B细胞刺激指数(SI)、NK细胞和CTL细胞杀伤活性;取瘤块,HE染色观察肿瘤细胞形态学变化.结果表明,PEA、PED各剂量组小鼠的生存质量较模型组提高,生命延长率最大分别为40.71％和51.33％;平均肿瘤体积较模型组显著减小,最大抑瘤率分别达50.06％和61.58％;T细胞和B细胞SI、NK细胞和CTL细胞杀伤活性较模型组显著升高.PEA、PED各剂量组正常肿瘤细胞减少,坏死面积增大,脂肪细胞增多.结果表明,PEA、PED可以提高H22肝癌小鼠的生存质量,延长生存期,抑制肿瘤生长,其机制与增强T细胞和B细胞转化能力,提高NK细胞和CTL细胞杀伤活性有关.

研究美洲大蠊(俗称蟑螂)提取物C Ⅱ-3纳米粒对免疫力低下小鼠免疫功能的改善作用.制备美洲大蠊提取物C Ⅱ-3纳米粒,并建立免疫功能低下小鼠模型,将小鼠随机分为正常对照组和模型组(灌胃生理盐水,0.2mL/10 g),辅料组(灌胃空白纳米粒,40 mg/kg,0.2 mL/10 g),阳性对照组(灌胃左旋咪唑,40 mg/kg,0.2mL/10 g),C Ⅱ-3组(灌胃美洲大蠊提取物C Ⅱ-3,40 mg/kg,0.2mL/10 g),C Ⅱ-3纳米粒5个剂量组(灌胃美洲大蠊提取物C Ⅱ-3纳米粒10、20、40、80、160 mg/kg,0.2 mL/10 g),各组连续给药15d后,检测小鼠外周血白细胞、红细胞、血小板;脏器指数;巨噬细胞吞噬功能;血清中免疫球蛋白IgG、IgM的含量.研究结果显示,CⅡ-3纳米粒各剂量组均可以显著提高免疫低下小鼠的白细胞数量(P＜0.05);能显著提高免疫力低下小鼠的肝脏、脾脏和胸腺指数(P＜0.01);可显著提高其巨噬细胞吞噬能力(P＜0.05)和免疫力低下小鼠血清中IgG、IgM的含量(P＜0.01),说明C Ⅱ-3纳米粒有改善免疫力低下小鼠免疫功能的作用.