Coptis chinensis Franch. is a perennial medicinal plant with huge economic and social benefits, but how altitude affects the accumulation of bioactive compounds through microbial ecosystems remains unexplored. This study examined how microbial communities at different altitudes influence the bioactive components of Coptis chinensis, to help identify beneficial microorganisms for application to its rhizomes. Samples of Coptis chinensis were cultivated at four different altitudes in Shizhu, Chongqing. To characterize the phytochemical profile of Coptis chinensis, nine specific alkaloids were quantified by High Performance Liquid Chromatography (HPLC) and Ultraviolet-Visible Spectrophotometry (UV-Vis), with Liquid Chromatography-Mass Spectrometry (LC-MS) subsequently employed to characterize differential metabolite accumulation at each altitude. Microbial community structure in the rhizomes was analyzed by metagenomic sequencing. Results indicated that the contents of groenlandicine, coptisine, berberine, and total alkaloids increased with altitude, with the total alkaloid content rising from 15.97% at 907 m to 17.82% at 1698 m (P < 0.01). Analysis revealed 912 differential metabolites, with distinct accumulation patterns at different altitudes. Microbial diversity in the rhizomes also varied by altitude, with significant shifts in Mucoromycota, Pseudomonadota, Rhizophagus, and Mesorhizobium populations. Moreover, the relative abundance of these microorganisms was intricately linked to alkaloid content. High altitude significantly enhances alkaloid accumulation in C. chinensis, and this effect is primarily mediated by the enrichment of beneficial endophytes, which promote the biosynthesis of target alkaloids via optimizing nitrogen utilization and inducing the expression of key enzymes.

ETHNOPHARMACOLOGICAL RELEVANCE:Reynoutria japonica has been utilized as a potential treatment for gastrointestinal disorders in Traditional Chinese Medicine (TCM). However, the efficacy and mechanism of its aqueous extracts on damp-heat ulcerative colitis (UC) remains unclear. AIM OF THE STUDY:Investigating the modulating effect of aqueous extracts of Reynoutria japonica (AERJ) on gut microbiota and fecal metabolisms in mice with damp-heat UC. METHODS:UPLC-Q-TOF-MS was used for the compositional characterization of the AERJ. BALB/c mice were used as a model for establishing damp-heat UC. Hematoxylin-eosin (HE) staining and immunohistochemistry (IHC) method were conducted to observe the pathological changes in the colon. Enzyme-linked immunosorbent assay (ELISA) was used for quantitative analysis of relevant physiological indicators. 16 S rRNA gene sequencing was employed to detect changes in gut microbiota in mice. In addition, metabolomics analysis was performed on mice feces. Finally, the aforementioned indicators underwent correlation analysis. RESULTS:Seven components within the AERJ were qualitatively identified by chromatographic analysis. The AERJ alleviated the symptoms in damp-heat UC mice, suppressed the expression of inflammatory factors, attenuated mucosal damage, improved water-fluid metabolism, and regulated the gut microbiota composition. Differential metabolites in feces were mainly enriched in the amino acid metabolic pathway, and AERJ was able to upregulate the expression levels of short chain fatty acids (SCFAs). Meanwhile, the present study found that physiological indicators were significantly correlated with both the gut microbiota and SCFAs. CONCLUSION:AERJ showed significant therapeutic effects on damp-heat UC mice, and its mechanism of action may be related to altering the composition of gut microbiota, regulating amino acid metabolism and the content of SCFAs and thus restoring the mucosal integrity and inflammatory response.

(1) Background: Polygonatum cyrtonema is a medicinal plant, and its polysaccharides are used for immunomodulation and the treatment of hyperglycemia. Investigation of the tissue distribution and pharmacokinetics of P. cyrtonema polysaccharide can further elucidate its pharmacological mechanisms. (2) Methods: A fluorescence-labeling approach using rhodamine B (RhB) as a fluorescent molecular probe was used for the quantitative assessment of the polysaccharide from dried P. cyrtonema (DPC1) samples, and the pharmacokinetics and tissue distribution of DPC1 were evaluated in mice after intraperitoneal or oral administration. (3) Results: DPC1 was successfully labeled with RhB, showing degrees of fluorescence labeling at 0.453% and 0.568% as determined by the ultraviolet and enzyme marker methods, respectively. DPC1-RhB was rapidly absorbed into the bloodstream after oral and intraperitoneal administration. Pharmacokinetic characteristics showed that oral administration and intraperitoneal administration were consistent with the features of a two-compartment model. (4) Conclusion: After administration, DPC1-RhB was primarily distributed in the tissues of the heart, spleen, and lung, indicating that the drug has a targeted effect on these tissues. Overall, the findings provide a comprehensive reference for the in vivo distribution of DPC1, together with a foundation for further elucidation of its pharmacological mechanisms and the development and application of DPC1 formulations.

Abstract The full text of this preprint has been withdrawn by the authors due to author disagreement with the posting of the preprint. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author.

Abstract Polygonatum cyrtonema is a medicinal plant and its polysaccharides are used for immunomodulation and the treatment of hypoglycemia. Investigation of the tissue distribution and pharmacokinetics of P. cyrtonema polysaccharide can further elucidate its pharmacological mechanism. A fluorescence labeling approach using rhodamine B (RhB) as a fluorescent molecular probe was used for the quantitative assessment of the polysaccharide from dried P. cyrtonema (DPC1) samples, and the pharmacokinetics and tissue distribution of DPC1 were evaluated in mice after intraperitoneal or oral administration. DPC1 was successfully labeled with RhB, showing degrees of fluorescence labeling at 0.453% and 0.568% as determined by the ultraviolet and enzyme marker methods, respectively. DPC1-RhB was rapidly absorbed into the bloodstream after oral and intraperitoneal administration. The relative bioavailability of DPC1-RhB was as high as 48.648%, showing linear pharmacokinetic characteristics. After administration, DPC1-RhB was primarily distributed in the tissues of the heart, spleen, and lung, indicating that the drug has a targeted effect on these tissues. Overall, the findings provide a comprehensive reference for the in vivo distribution of DPC1, together with a foundation for further elucidation of its pharmacological mechanism and the development and application of DPC1 formulations.

目的 建立细风轮菜的质量标准.方法 收集10批细风轮菜药材样品,对其进行外观性状、显微鉴别和薄层鉴别,并对其水分、总灰分、酸不溶性灰分、稀乙醇浸出物进行检查;采用高效液相色谱法测定药材中迷迭香酸的含量.结果 细风轮菜药材茎纤细,呈方柱形,被白色绒毛,表面为灰绿色或绿褐色;茎横切面可见表皮细胞、非腺毛、皮层细胞等;粉末可见非腺毛、导管、木纤维、叶肉细胞等.薄层鉴别结果显示,供试品色谱中,在与醉鱼草皂苷Ⅳb对照品色谱相应的位置上,显相同颜色的斑点.10批样品的水分、总灰分、酸不溶性灰分、稀乙醇浸出物、迷迭香酸含量分别为8.69％～12.33％、5.96％～13.33％、0.14％～3.29％、18.57％～32.61％、0.35％～0.82％,平均值分别为10.10％、9.73％、1.06％、23.54％、0.56％.结论 所建方法可用于细风轮菜药材的质量控制.初步拟定细风轮菜药材中水分不得过12.0％,总灰分不得过12.0％,酸不溶性灰分不得过1.5％,稀乙醇浸出物不得少于18.0％,迷迭香酸含量不得少于0.45％.

Plant phenolic compounds have attracted considerable attention because of their health benefits. This study aimed to investigate the composition and antioxidant activity of phenol extracts from Polygonatum zanlanscianense Pamp. steam and leaf (PPP). The FTIR, UPLC-Q-Obtrip-MS, and HPLC−DAD methods were used to analyze the composition of PPP, and 20 phenolic compounds were preliminarily identified. Among them, the contents of hyperin, astragalin, and diosmetin levels were the highest. Treatment with PPP can significantly reduce t-BHP-induced cell damage in HepG2 cells, reactive oxygen species (ROS) accumulation, and malondialdehyde (MDA) content. Meanwhile, the superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and glutathione peroxidase (GSH−Px) activities can be increased. Moreover, PPP enhanced Nrf2 expression, which was consistent with that of heme oxygenase-1 (HO−1), glutamate–cysteine ligase catalytic subunit (GCLC), and NAD(P)H quinone oxidoreductase-1 (NQO1), whereas the expression of Keap1, the Nrf2 inhibitor, was decreased. All findings indicate that PPP can serve as a natural bioactive substance for preventing oxidative stress.

目的:研究齐墩果酸(OA)对脾虚水湿内停模型小鼠的水液代谢功能的影响及其作用机制.方法:将60只SPF级昆明种小鼠随机分为空白组(10只)和造模组(50只),造模组采用"久居湿地+饮食失节"的方法建立脾虚水湿内停模型.模型复制成功后将造模组小鼠随机分为模型组、自然恢复组和OA低、中、高剂量组,共5组,每组10只.空白组、模型组、自然恢复组给予10mL·kg-1·d-1生理盐水灌胃,OA低、中、高剂量组分别给予50、100、200 mg·kg-1·d-1 OA灌胃,持续给药7d.造模和给药前后观察小鼠的一般体征,测定小鼠体质量,使用烘干法测定小鼠粪便含水率和组织含水量,称量法检测水负荷指数和脏器系数,酶联免疫吸附测定法(ELISA)检测尿D-木糖排泄量、血清胃泌素(GAS)、总蛋白(TP)、白蛋白(ALB)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、白细胞介素-6(IL-6)、抗利尿激素(AVP)、肾髓水通道蛋白1(AQP1)及肝脏Na+-K+-ATP酶等指标,并将OA与检测指标中的ALB、IL-6、AQP1和Na+-K+-ATP酶进行分子对接.结果:与空白组比较,模型组小鼠毛发疏松、消瘦、倦怠懒惰、动作迟缓,体质量增长缓慢、自主活动次数减少、粪便含水率和组织含水量增加、水负荷小鼠体质量下降值减少、各脏器系数明显升高(P＜0.05,P＜0.01);尿D-木糖排泄量明显减少,血清中GAS、TP、ALB和HDL-C的水平均明显降低,TC、LDL-C、AVP、IL-6的水平均明显升高,肝脏Na+-K+-ATP酶表达明显降低,肾髓AQP1表达明显升高(P＜0.05,P＜0.01).与模型组比较,OA各剂量组的一般体征明显好转,体质量增长加快,自主活动次数增加,粪便含水率和组织含水量降低,水负荷小鼠体质量下降值增加、各脏器系数明显降低(P＜0.05,P＜0.01);尿D-木糖排泄量增加,血清中GAS、TP、ALB和HDL-C的水平升高,TC、LDL-C、AVP、IL-6的水平降低,肝脏Na+-K+-ATP酶表达明显升高,肾髓AQP1表达明显降低(P＜0.05,P＜0.01).OA各剂量组的各指标恢复效果均强于自然恢复组.分子对接结果也表明OA与ALB、IL-6、AQP1和Na+-K+-ATP酶具有较强的结合亲和力.结论:OA能改善脾虚水湿内停小鼠的水液代谢异常,为其潜在临床新用途提供科学依据.

目的:通过探讨影响桑分布的主要气候因子及对桑进行适生区预测,为桑的引种栽培及桑资源的合理开发应用提供参考.方法:收集全国范围内桑的样本地理分布信息,运用最大熵模型(Max-Ent模型)分析出影响桑分布的主导气候因子,并结合ArcGIS软件对桑进行适生区分析.结果:建立的模型精度高,预测效果可靠(AUC=0.977).预测结果显示,影响桑分布的主导气候因子为12月太阳辐射量、最暖季降水量、2月最高温;桑在全国范围内几乎都有分布,桑的适生区(中适宜生境、高适宜生境)主要集中在四川东南部、甘肃南部、云南东北部、宁夏南部、海南北部、新疆西部、西藏东南部、内蒙古东南部、吉林西南部、陕西、重庆、贵州、广西、广东、湖南、湖北、河南、辽宁、山西、河北、河南、北京、天津、山东、江苏、上海、安徽、浙江、福建、台湾等地.结论:本研究预测的桑分布区域与实际情况大致吻合,可为桑的引种栽培、全面调查桑资源的分布及桑资源的合理利用提供参考.

Objective To analyze and compare the alkaloids in phloem and xylem of Coptis chinensis with different grades,and clarify the distribution rules of the components in different tissues of C. chinensis. Methods Taking the different commodity grades of C. chinensis as the research object, vernier caliper was used to measure proportion of phloem and xylem accurately, colorimeter was used to determine L * , a * and b * values of powder, and ultraviolet spectrophotometry was used to detecte the content of total alkaloids in rhizome, phloem and xylem. The content of magnoflorine, groenlandicine,columbamine, jatrorrhizine, epiberberine, coptisine, palmatine and berberine were analyzed by external standard method and multi-components single-marker method. Combined with analysis of variance, correlation analysis, principal component analysis and partial least squares discriminant analysis to compare the chemical compounds in phloem and xylem, and find the signature components. Results The larger diameter of the medicinal material had a relatively larger proportion of phloem and xylem. Among them, the proportion of third grade was 0.63, second grade was 0.78, first grade was 0.87 and special grade was 1.01. The color difference values L * , a * and b * of the rhizome and phloem were smaller than those of the xylem. The content of total alkaloids was 15.96% in rhizome, 16.68% in the phloem and 13.53% in the xylem, the alkaloid content in the phloem was significantly higher than that of the xylem（P ＜ 0.001）, and there was no significant difference in the total alkaloid content of different grades of C. chinensis. The contents of most compounds showed a decreasing trend with the increase of the commercial grade of C. chinensis, and there was no significant difference between the quantitative results of external standard method and multi-components by single-marker method. There was no significant correlation betweenthe alkaloid content of rhizome and weight, length and ratio of phloem to xylem, but there was a significant negative correlation between the coptisine content and diameter（P ＜ 0.05）. The color difference value L * was significantly negatively correlated with jatrorrhizine hydrochloride and coptisine（P ＜ 0.05）, extremely significantly negatively correlated with palmatine hydrochloride and berberine（P ＜ 0.01）. The color difference value a * was significantly positively correlated with magnoflorine（P ＜ 0.01）, and the color difference value b * was significantly negatively correlated with palmatine hydrochloride（P ＜ 0.05）. The color measurement values L * , a * , b * were negatively correlated with the content of alkaloids（P ＜ 0.01）. Principal component analysis indicated that the cumulative variance contribution rate of three principal components were 81.59%.According to the orthogonal partial least squares-discriminant analysis, four different index compounds were found, namely coptisine, berberine, columbamine and palmatine. Conclusion The distribution of alkaloids in different tissues of C.chinensis was clarified by means of colorimeter, ultraviolet spectrophotometry, high performance liquid chromatography and chemometrics analysis, with view to providing the scientific basis for the “quality evaluation through morphological identification” of C. chinensis.

目的:研究黄精、玉竹、桑叶配方(HYS)对抗生素相关性腹泻(AAD)大鼠的恢复作用及对肠道菌群的调节作用.方法:大鼠连续3天灌胃盐酸林可霉素(10mL/kg),建立大鼠AAD模型.给予AAD大鼠中药配方(HYS)和双歧三联活菌(Bifid triple viable,BTV),给药7天后,显微镜下观察肠切片,采用Thermofisher Scientific Coated ELISA Kit测定炎症因子,Illumina测序测定肠道菌群.结果:AAD组肠黏膜切片可见大量炎性细胞浸润,炎症因子TNF-α、IL-4、IL-6明显高于正常对照组(NC组)(P＜0.05),AAD组肠道菌群的多样性和丰富度显著低于NC组(P＜0.05).结论:HYS可显著恢复AAD大鼠肠黏膜形态,降低TNF-α、IL-4、IL-6含量,缓解炎症症状,恢复肠道菌群的多样性和丰富度,可能与拟杆菌、粪杆菌、乳酸菌及瘤胃球菌科_UCG-005和毛螺菌科_NK4A136_组密切相关.

Objective: According to Traditional Chinese Medicine theory, spleen deficiency and phlegm-dampness syndrome (SDPDS) are caused by abnormal water metabolism in the body because of spleen dysfunction. The purpose of this article was to evaluate the efficacy of D-limonene (DL) in resolving phlegm in mice with SDPDS from the perspective of regulating the level of aquaporin 3 (AQP3). Methods: The model of SDPDS was induced in mice using the multifactor modeling method, which combines internal and external dampness. An artificial climate box was used to create a humid environment, whereas the irregular diet was caused by different feeding methods on odd-even days. The mice were divided into blank control, model group, DL low-dose, DL high-dose, and positive groups. The mice were modeled and treated for 7 day. Levels of gastrin and amylase (AMS) in the serum, mucus secretion in the trachea, and AQP3 in the tissue near the gastric cardia. Results: DL significantly reduced mucus secretion in the trachea ( P < 0.001). It also increased the level of AMS in the serum ( P < 0.01) and decreased the level of AQP3 in the gastric tissue ( P < 0.01). Conclusions: Mice with SDPDS exhibited disturbed water metabolism and significantly increased AQP3 levels. DL can restore the levels of AQP3 and plays an important role in resolving phlegm. This study may also help expand the efficacy of natural drugs containing DL and improve the utilization of natural drug resources.

Chuanxiong (CX, Ligusticum chuanxiong), Japanese Chuanxiong (JCX, Cnidium officinale), Fuxiong (FX, Ligusticum sinense ‘Fuxiong’), and Jinxiong (JX, Ligusticum sinense ‘Jinxiong’) are aromatic herbs used in China, Japan, and other regions. Their morphology and aromatic odor are similar, resulting in confused and mixed uses. This study compares the volatile compositions of these herbs for defining their medical uses. Headspace solid-phase microextraction–gas chromatography–triple quadrupole–mass spectrometry was employed to separate, identify, and quantify the compounds in the volatile gas of the four herbs. A total of 128 volatile compounds were identified and quantified in 23 these herbal samples. The sums of 106, 115, 116, and 120 compounds were detected in the volatile gas of CX, JCX, FX, and JX, with the mean contents of 4.80, 7.12, 7.67, and 12.0 μg/g, respectively. Types and contents of the main compounds were found to be different in the volatile gas of these herbs. The orthogonal partial least squares discriminant analysis and hierarchical clustering analysis showed the four herbs located in different confined areas or clusters. It is concluded that the volatile compositions in the four herbs are generally similar, but the contents of main volatile compounds are different. These herbs should be clearly differentiated in medical use.

目的:为藏药丝瓜子质量标准的建立提供参考.方法:采用性状鉴别、显微鉴别和TLC方法进行药材鉴别研究,进行水分、总灰分和浸出物检查,采用高效液相色谱法(HPLC)建立药材中亚油酸的含量测定方法.结果:丝瓜子药材性状特征明显,且丝瓜子与广东丝瓜子性状差异较大;粉末呈灰绿色,显微特征清晰,可见种皮表皮细胞、种皮下皮细胞、石细胞、导管、子叶细胞和内种皮星状细胞等;TLC图斑点清晰,分离度良好;9批丝瓜子药材的水分含量为5.96％～9.89％,总灰分含量为2.44％～3.75％,醇溶性浸出物含量为23.64％～30.72％;HPLC法测定亚油酸在0.056～1.13 mg mL1(R2=0.9994)范围内线性关系良好,精密度、稳定性、重复性试验的RSD＜2.0％(n=6),加样回收率为97.24％～106.07％(RSD=2.90％,n=6).结论:初步拟定藏药丝瓜子中水分、总灰分分别不得过13.0％、4.0％,醇溶性浸出物不得少于20.0％,亚油酸含量不得少于2.5％.此研究结果为藏药丝瓜子的质量控制提供了科学的实验依据.

The quality of Paeoniae Radix Alba and Paeoniae Radix Rubra is evaluated by root thickness, and paeoniflorin serves as a common quality indicator of them. However, the correlation between the content of bioactive compounds and the root size is still unclear. Therefore, this study characterized the distribution patterns and content of seven bioactive compounds including paeoniflorin in different tissues of Paeonia lactiflora roots, analyzed the correlation between the root size and the content of bioactive compounds based on the xylem-to-bark ratio, and further determined the index components for quality assessment. Nine samples of fresh P. lactiflora roots were collected from the genuine cultivation area. The distribution of bioactive compounds in different tissues on the cross-section of the root was firstly analyzed by desorption electrospray ionization-mass spectrometry imaging(DESI-MSI). Subsequently, the content of bioactive compounds was determined in the xylems and barks of the roots by UPLC. The compounds with the largest difference between the xylem and the bark were selected by orthogonal partial least squares discriminant analysis(OPLS-DA). The results indicated that paeoniflorin, benzoylpaeoniflorin, oxypaeoniflorin, gallic acid, and 1,2,3,4,6-pentagalloylglucose were significantly accumulated in the xylems, while albiflorin and catechin were mainly distributed in the barks. Paeoniflorin and albiflorin, with the largest differences in the xylem and the bark, had the highest content in the two tissues. The root diameter was positively correlated with paeoniflorin content and negatively correlated with albiflorin content. As isomers with different efficacies, paeoniflorin or albiflorin can be chosen as the quality marker corresponding to specific clinical application to launch quality classification evaluation of multi-functional Chinese medicines.

目的 提出一种基于"外来药物-药理作用-传统中药-药性"关联网络研究外来药物药性的新方法,并以玛咖为例对其进行阐释与验证.方法 首先整理玛咖药理作用的综述类文献,建立玛咖与其药理作用的关联,并查找相关的用药规律分析文献,建立玛咖的药理作用与传统中药的关联,再以《中国药典》或《中药学》教材为依据获取传统中药与药性的关联,最终构建出"玛咖-药理作用-传统中药-药性"关联网络,进一步从该网络中解拆出每个药理作用的"药理作用-传统中药-药性"关联网络并进行网络拓扑分析,再通过处理相关数据计算玛咖的药性占比.结果 本研究的结果与现有研究基本一致,玛咖的药性应为:性微温,味甘、辛、苦;归肾、肝、脾经;功效是滋肾益精,疏肝止痛,健脾补气.结论 本研究所提出的方法有助于外来药物中药化的进程,为拓展中药新资源提供了一种新思路.

目的:基于网络药理学预测厚朴-广藿香药对治疗新型冠状病毒肺炎(Corona Virus Dis-ease 2019,COVID-19)的作用靶点及信号通路,探讨其作用机制.方法:通过TCMSP,BATMAN-TCM数据库查询和相关文献检索筛选出厚朴-广藿香的活性成分及对应作用靶点,经过Uniprot数据库查询靶点对应的基因,将其标准化;利用GeneGards数据库对COVID-19相关靶点进行预测,将两者进行映射,借助ImageGP在线平台绘制维恩图,筛选药物疾病共有靶点;利用Cytoscape3.7.2软件构建"药物-活性成分-疾病靶点"网络;通过STRING数据库获取靶标蛋白相互作用网络关系并利用Cytoscape3.7.2软件构建PPI网络.最后利用Metascape数据库进行GO富集分析与KEGG通路分析,借助ImageGP在线平台对结果进行可视化处理.结果:根据条件筛选得到厚朴-广藿香活性成分15个和227个潜在作用靶点,其中与COVID-19共同靶点47个.GO功能富集分析得到GO条目4854个(P<0.01),其中生物过程(Biological Process,BP)条目4178个,细胞组分(Cellular Component,CC)条目311个,分子功能(Molecular Function,MF)条目365个.KEGG通路富集筛选得到187条信号通路(P<0.01),涉及IL-17信号通路、NF-κB信号通路、MAPK信号通路、细胞凋亡等.结论:厚朴-广藿香中主要潜在活性成分桉树醇、厚朴酚、葛花苷元、槲皮素等可能作用于IL6、TNF、TP53、CASP3、MAPK1、IL10、CCL2等关键靶点调节多条信号通路,从而起到治疗COVID-19的作用.

A new phenolic glycoside, 7-(4- O- β-D-glucopyranosyl, 3-methoxyphenyl)-6,8-dihydrofuro[3′,4′:7,8]naphtho[1,2-d][1,3]dioxol-10(7H)-one, named malconenoside A (1), was isolated from Bupleurum malconense Shan et Y. Li . The structure, including absolute configurations, was assigned by using spectroscopic methods and ECD calculation. Biological activities of compound 1 towards human cancer cells (HepG2, BGC-823, and A549) were evaluated. The most sensitive two cell lines were HepG2 and BGC823, in which the IC 50 values of compound 1 were 8.70 and 16.94 μM, respectively.

目的 建立藏药川西合耳菊Synotis solidaginea中黄酮类和酚酸类药效成分含量测定的方法,并评价不同产地、不同部位样品的质量.方法 建立HPLC测定川西合耳菊中芦丁、金丝桃苷、异槲皮苷、烟花苷、紫云英苷、绿原酸、异绿原酸A、异绿原酸C8种有效成分含量的方法.从西藏、四川和青海收集了19份川西合耳菊药材样品;将全株植物分成茎、叶、花3个部位,测定这些样品中8种成分的含量.运用主成分分析法比较不同产地药材中的药效成分,以及植物不同部位之间和同一部位不同产地样品之间差异.结果 这8种药效成分在40min内分离良好,加样回收率为96.3％～104.9％.19份川西合耳菊样品中3种酚酸类成分的总量为5种黄酮类成分总量的2.77倍.酚酸类以绿原酸的含量最高(9.74 mg/g);黄酮类以芦丁的含量最高(3.65 mg/g).根据药效成分含量和主成分分析,四川道孚的样品质量更好.植物3个部位之间比较,总量以叶中含量最高,占59.15％～65.61％;其次为花,占30.52％～34.31％;茎中的含量最低,仅占3.9％～5.7％.金丝桃苷在花中的含量高,而其他7种成分在叶中的含量最高.结论 建立的方法能准确测定川西合耳菊中5种黄酮类和3种酚酸类成分的含量,评价其质量.川西合耳菊叶和花的药效成分含量高,应于开花期、叶茂盛时采集,并防止叶和花脱落,以保证质量.