介绍了反渗透技术在菲律宾ILIJAN电厂水处理系统中的应用情况,重点介绍了ILIJAN电厂水处理系统中的反渗透膜更换为Vontron反渗透膜后的运行情况。

In order to investigate immunization schedules of classical swine fever(CSF) in large-scale pig farms,and to evaluate the immune status,seven large-scale pig farms in Hubei province were selected.Pigs were divided into the sows,the boars,the prepubertal gilts,the breast-feeding piglets(about 0-30 days),the nursing piglets(about 31-60 days old) and the growing pigs(above 61 days old).In each herd,blood were collected from 5%-8% of pigs and antibody titers were tested.Results showed that the qualification rate of all immunized herds were 99.55%,96.43%,99.22%,89.88%,81.68% or 78.64%,and different immunization schedule induced different immune status.Overall,the nursing piglets and the growing pigs were the weakest of all pig groups.Piglets received primary vaccination on day 14,21,28 and 35 after birth based and the antibody examined 7 and 14 days after immunization.Results indicated that 28 days after birth was the best time for primary immunization.This study provides valuable references to the control of CSF.

反渗透作为一种重要的水处理技术,越来越广泛地应用到工业的很多领域.上海焦化厂以黄浦江水为原水,用反渗透技术进行深度处理,介绍了VONTRON抗污染系列的反渗透膜在焦化厂锅炉补给水项目中膜元件替换方面的应用情况.

According to the Bordetella bronchiseptica fimD sequence(X75811) of GenBank,we designed a pair of primer,and 1 098 bp DNA fragment of fimD was amplified from swine Bordetella bronchiseptica identificated in our laboratory.The acquired PCR products were inserted into pET-28a and expressed in BL21(DE3).Results of Sodium docecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) and Western blot assay showed that the fimD gene was expressed in the form of inclusion body,and the recombinant protein was about 42 kD,and can react with the swine antisera to Bordetella bronchiseptica.The fusion protein was purified and used as coating antigen to develop the indirect Enzyme-Linked Immunosorbent Assay(ELISA).When using this ELISA to detect 668 clinical sera,30.7% positive sera were detected.Parallel examination of the same 102 serum samples was conducted by using the ELISA and micro-agglutination test(MAT),the result showed that the ELISA is more sensitivity than MAT.

OBJECTIVE To construct an attenuated Salmonella choleraesuis vaccine strain expressing the gene SLT-IIeB and FedF of Shiga-like toxin Escherichia coli (SLTEC) O138 with balanced lethal system. METHODS The gene of SLT-IIeB and FedF of SLTEC O138 was amplified, and then recombined with a vector pYA3493 (Asd+). The recombinant was electroporated into attenuated Salmonella choleraesuis C500 (Asd-). The expression of SLT-IIeB and FedF was analyzed by SDS-PAGE. The stability of the vaccine strains was studied by generation culture in vitro. RESULTS The stable attenuated Salmonella choleraesuis vaccine strain expressing SLT-IIeB and FedF of SLTEC O138 was constructed with balanced lethal system. The expressed products with protein quality 37000 could react with the antibody of FedF and SLT-IIeB. CONCLUSION The attenuated Salmonella choleraesuis vaccine strain could probably serve as a vaccine against edema disease and piglets paratyphoid.

A nested polymerase chain reaction(N-PCR) assay for direct detection of toxigenic Pasteurella multocida(T+ Pm) in nasal swab specimens collected from pigs was developed using 2 pairs of primers that were designed according to the published sequences of T+ Pm toxA,The size of amplicons were 864 and 447 bp,respectively.The assay could detect 26 CFU microorganisms.Mean while,no false amplification in detection of Escherichia coli,Streptococcus,Staphylococcus,Bordetella bronchiseptica,Nontoxigenic Pasteurella multocida,Haemophilus parasuis and Actinobacillus pleuopheumoniae could be observed.The nested PCR and bacterial isolation were employed simultaneously to examine 146 nasal swabs collected from 5 herds known to be infected with toxigenic Pasteurella multocida,44 and 97 specimens were positive and negative for both isolation and nested PCR,respectively,5 were positive for nested PCR only.The coincidence between bacterial isolation and nested PCR is 96.58%.These results indicated that the developed nested PCR might be a promising approach in clinical screening and detection of toxigenic Pasteurella multocida in a large number of swine swabs.