Promoter is an important component of genes that determines the transcription of mRNA to achieve the orderly development and differentiation of organisms. In present study,we identified 1 341 promoters in silkworm( Bombyx mori) genomic and full-length cDNA sequence databases. Sequence analysis showed that GC content and AT content had one and two peak values in the promoter region from- 500 to 200 bp,respectively. Elements of TATA-box,Initiator( Inr),CAAT-box and GC-box were present in 256,453,200 and 334 promoters,respectively. The first three elements were dominantly located in the region from- 500 to 200 bp,and the last element was mostly located at the upstream far from- 1 600 to- 300 bp. Silkworm-based genomic microarray data showed that 23 promoters are for testis-biased gene expression. Cis-element analysis indicated that these promoters for testis-biased expression had 6 predominant motifs,among which 3 motifs had significantly higher occurrence than the promoters for non-tissue specific expression,suggesting that these 3 motifs play an important role in the regulation of testis-biased gene transcription.
Prepositions are the most basic words people employ to express spatial concepts,and they are also an important part of English language.Based on the prototype theory,this paper is to analyze the semantic structure of to in a systematic and comprehensive way and to prove the fact that every sense of a preposition is from the prototypical features of the word.Thus,it is aimed to give some implications for English teaching.
通过正交试验,筛选制备左氧氟沙星缓释颗粒剂,紫外分光光度法测定其体外释放度.采用高效液相法色谱法测定其在鸡体内血药浓度.结果:缓释颗粒剂的最佳处方是左氧氟沙星50%,相对分子质量为20万Da的HPMC 15%,乳糖30%,PVPK30适量.缓释颗粒剂体外释放符合Higuchi方程.其药动学参数为:Cmax为13.33μg/mL,Tmax为8 h,AUC,为253.68μg/mL,t1/2为7.96 h.相对生物利用度93.7%.结论:左氧氟沙星缓释颗粒剂在体内外均具有明显缓释特征.
质的研究与量的研究是社会科学研究领域的两大范式.教育学者对于教育研究中应采用质的研究还是量的研究更为科学有着激烈的论争,同时也企图寻找结合的路径.但通过分析教育研究者在实践中对质的研究与量的研究的运用,看到的却是教育研究者对方法论及其方法的忽视.因此涵养科学精神才是教育研究者必须且迫切的选择.
Tourism constructs the reality that tourist coming from different places are exchanging cross-cultural and intercultural communication.And this provides condition for shaping the tourists' new cultural personality,which is patulous and multiplex cultural personality by tourism.The motivation,basic concept of culture and degree of socialization of tourists are key factors affecting tourists to form their culture personality.So developing a cultural behavior perspective for tourists from tourism,eliminating the concept of racialism and improving the cross-cultural ability would fairly exert the function of tourism on shaping tourists' cultural personality.
韩国梨花女子大学是韩国最负盛名的私立女子大学,同时也是世界最大的综合性女子大学,拥有丰富且成功的女性教育经验,可给予我国女性高等教育多方面的启示.