Two kinds of interplanting modes tea and tea-persimmon were set up to analyze the soil nutrients and other environmental factors,the quality of tea and economic benefit.The results showed that compared with pure tea mode,the humidity of the interplanting modes of tea-persimmon were increased by 13.6%,18.6% on the surface and near earth 30 cm in summer.However the temperature were down 4.0℃ and 0.5℃,light intensity reduced 660.22 μmol·m-2·s-1,wind speed reduced 75% and available phosphorus and available kalium contents of the soil were increased by 91.7%,150.0%.Water extract of the spring and summer tea,caffeine,amino acid,soluble sugar,soluble protein content of tea-persimmon interplanting modes were higher than pure tea garden.

The effects of organic substances,such as thiamine hydrochloride,phaseomannite,yeast extract,L-cysteine,methionine,L-leucine,and L-α-alanine,on the growth of and coenzyme Q10(CoQ10) synthesis in hairy root of cv.Zhongyan 99 were studied with liquid suspension culture.The results showed that: when concentration was suitable,thiamine hydrochloride,L-cysteine,methionine,L-leucine and L-α-alanine promoted the growth of and CoQ10 synthesis in hairy root obviously;while the effect of yeast extract was negative;phaseomannite positively affected the growth of hairy root,but did not improve the synthesis of CoQ10.

The effects of several elicitors,including mannitol,cellulase,pectinase,salicylic acid,eggplant verticillium wilt and tomato fulvia fulva on tobacco (NC89) cell growth and synthesis of coenzyme Q10 were compared. The results showed that proper concentration of mannitol was benefit to NC89 cell growth,but it would inhibit the intracellular synthesis of coenzyme Q10;when the concentration of mannitol was 5.0 mg/L,cell’s dry weight increased by 14.6%,while the production of coenzyme Q10 only increased by 1.9%. Salicylic acid,pectinase and tomato fulvia fulva promoted the intracellular synthesis of coenzyme Q10 though certain inhibition to tobacco cell growth. When the concentrations of salicylic acid and tomato fulvia fulva were 5.0 mg/L and 25 mL/L respectively,the content of intracellular coenzyme Q10 increased by 25.8% and 66.1%,but the production of coenzyme Q10 decreased by 7.0% and 1.9% respectively. The content and production of coenzyme Q10 could be improved by 54.0% and 40.0% respectively with the concentration of pectinase at 3 U/L. Eggplant verticillium wilt elicitor and cellulase were beneficial to both cell growth and synthesis of intracellular coenzyme Q10,when their concentrations were 25 mL/L and 2 U/L,the production of coenzyme Q10 increased by 71.1% and 36.6%,respectively.

The effects of precursors,such as coenzyme Q0,p-hydroxybenzoic acid, solanesol,L-tyrosine,L-phenylalanine and methionine,on the dry weight of suspension cell,Q10 production and intracellular Q10 content in tobacco NC89 were studied with cell culture method.The results showed that: the addition of precursors could increase cell's dry weight and intracellular Q10 content to a certain extent,especially p-hydroxybenzoic acid and methionine had significant effects on cell's dry weight.The coactions of either p-hydroxybenzoic acid or L-phenylalanine,together with methionine extremely significantly affected Q10 content.The production of Q10 changed along with cell's dry weight and intracellular Q10 content.Therefore,it was allowed to apply selected precursors of proper concentration stepwisely at different cell culture phases.

This article was a review about the characters of Agrobacterium rhizogenes,the structure and function of its Ri plasmid,the transferring method of hairy root and factors influencing transformation,the methods of culture and identifying hairy root and the application of Ri plasmid to plant secondary metabolites.

发根农杆菌(Agrobacterium rhizogenes)属于根瘤菌科(Rhizobitaceae)农杆菌属(Agrobacterium)的革兰氏阴性菌,具有侵染绝大多数的双子叶植物和少数单子叶植物以及个别的裸子植物的生物学特性.

In the article the categories of elicitors and its mechanism of plant induced disease resistance were mainly described.Also the application foreground of elicitors was expected.