Following the publication of this article, a concerned reader drew to the Editor's attention that the experimental design of the western blot assay experiments portrayed in Fig. 5A on p. 7804, and the overall organization of this figure, may have been flawed, as mitochondrial and cytosolic proteins were featured in the figure with only one set of supporting control western blot data; in this scenario, the proteins would necessarily have needed to have been obtained from two separate cell samples in different experiments, and blotted on to separate gels. Moreover, there was also a concern that certain of the gels featured possible breaks in their continuity/splicing events, such that the protein bands in the figure were not shown consecutively, as they would have appeared, in the affected slices. After having conducted an internal investigation, the Editor of Molecular Medicine Reports agrees with the reader that there were anomalies associated with the presentation of Fig. 5. Therefore, on the grounds of a lack of confidence in the presented data, the Editor has decided that the article should be retracted from the publication. The authors were asked for an explanation to account for these concerns, but the Editorial Office did not receive a reply. The Editor apologizes to the readership for any inconvenience caused, and we also thank the reader for bringing this matter to our attention. [Molecular Medicine Reports 17: 7797‑7806, 2018; DOI: 10.3892/mmr.2018.8823].

目的 探讨间隙水分离结合点状精准止血法在卵巢子宫内膜异位囊肿剥除术中的应用价值.方法 选取2018年6月～2021年12月在北京同仁医院妇产科行腹腔镜下双侧卵巢子宫内膜异位囊肿病人56例,年龄20～35岁.依据随机数字表法将病人分为研究组(间隙水分离结合精准点状止血组)和对照组(直接剥除结合常规止血组),各28例.比较两组病人术前、术后1月基础状态卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)、抗苗勒管激素(AMH)水平及窦状卵泡计数(AFC)的差异,观察手术对卵巢功能的影响.结果 对照组术后1个月与术前相比,E2和AMH均明显下降,而FSH和LH明显升高,手术前后差异均有统计学意义;两组术后1个月血FSH、LH、E2、AMH水平比较,组间差异有统计学意义.研究组术后1个月AFC较对照组高,组间差异有统计学意义.结论 应用间隙水分离法行腹腔镜下卵巢子宫内膜异位囊肿剥离,剥离过程中发现出血点即采用点状精准电凝法止血,简单安全,可有效地保护卵巢功能,有一定的临床应用价值,值得推广.

Objective: To compare the efficacy and safety of radical surgery with radiotherapy in patients with early-stage cervical carcinoma. Methods: PubMed, Web of Science, and Embase were systematically reviewed, and studies comparing radical surgery with radiotherapy were included. The main efficacy outcomes included overall survival (OS), and disease-free survival (DFS). Safety endpoints were adverse events. Hazard ratios (HR) or risk ratios (RR) with 95% CI were used to pool the estimates. Results: A total of 6 studies were included in this meta-analysis. Radical surgery was associated with comparable survival effects in OS (HR = 0.73; 95% CI 0.46–1.17; p = 0.196) and DFS (HR = 0.84; 95% CI 0.64–1.10; p = 0.207) as compared with radiotherapy. Moreover, positive lymphangiography (HR = 3.67; 95% CI 2.86–4.70; p < 0.001), adeno-carcinomatous histotype (HR = 2.53; 95% CI 1.80–3.56; p < 0.001), adenosquamous histotype (HR = 1.55; 95% CI 1.27–1.89; p < 0.001), tumor size ≥4 cm (HR = 1.60; 95% CI 1.14–2.23; p < 0.001), stage IB2 (HR = 1.71; 95% CI 1.43–2.04; p < 0.001), and stage IIA (HR = 1.85; 95% CI 1.54–2.22; p < 0.001) were all independent predictors of decreased survival. Patients treated with radical surgery had a rate of adverse events similar to that of those treated with radiotherapy (RR = 1.23; 95% CI 0.61–2.48; p = 0.557). Conclusion: The present study suggested that radical surgery and radiotherapy offered similarly effective treatment in terms of OS and DFS in early-stage cervical carcinoma. Moreover, the complication rate between the 2 treatments was not significantly different. Considering the potential limitations of this study, more large-scale well-designed randomized controlled trials are needed to verify our findings.

目的:探讨桂枝茯苓胶囊联合屈螺酮炔雌醇片治疗复发性卵巢子宫内膜异位囊肿的临床疗效.方法:回顾性选取2018年1月至2021年1月在北京同仁医院就诊的复发性卵巢子宫内膜异位囊肿患者62例,根据用药情况分为对照组和观察组,每组31例.对照组患者于月经来潮第1日开始每晚睡前口服屈螺酮炔雌醇,连服21 d,停药7 d开始下1个周期;观察组患者在对照组的基础上于三餐后口服桂枝茯苓胶囊.两组患者均连续治疗6个月经周期.比较两组患者的临床疗效和用药安全性的差异,观察两组患者治疗前后的疼痛视觉模拟评分(visual analogue scale,VAS)、盆腔包块直径以及CA125水平.结果:观察组、对照组患者的总有效率分别为95.16％(59/62)、80.65％(50/62),观察组患者的临床疗效明显优于对照组,差异有统计学意义(P<0.05).与治疗前相比,两组患者治疗后的VAS评分明显降低,盆腔包块直径明显缩小,CA125水平明显降低,且观察组患者上述指标明显优于对照组,差异均有统计学意义(P<0.05).对照组、观察组患者的不良反应发生率分别为9.68％(6/62)、8.06％(5/62),差异无统计学意义(P>0.05).结论:桂枝茯苓胶囊联合屈螺酮炔雌醇片治疗复发性卵巢子宫内膜异位囊肿,可明显改善患者痛经和慢性盆腔痛,缩小盆腔包块直径,提高患者的生活质量,有一定的临床应用价值.

目的 测定不同级别宫颈鳞状上皮内病变(SIL)中p16、Ki-67、P53蛋白的表达水平,探讨联合检测上述生物学标记物对HSIL的辅助诊断价值.方法 回顾性分析2015年3月-2018年3月首都医科大学附属北京同仁医院阴道镜活检病理标本共180例,根据病理组织学诊断结果分为HSIL组、LSIL组及宫颈炎组,每组各60例,其中宫颈炎组作为对照组.采用免疫组化SP法检测p16、Ki-67、P53表达情况,判断检测方法的灵敏度、特异度,评价联合检测的诊断效能.结果 p16在HSIL组、LSIL组及宫颈炎组的阳性率分别为95.00％、73.33％、15.00％;Ki-67阳性表达率分别为86.67％、65.00％、8.33％;P53阳性表达率分别为96.67％、80.00％、8.33％.p16、Ki-67和P53阳性表达率随宫颈病变级别的升高而呈上升趋势,三组比较差异有统计学意义(P<0.01);p16、Ki-67和P53三种生物学指标联合应用并联灵敏度95.6％,特异度85.5％;三者串联灵敏度81.6％,特异度100％.结论 p16、Ki-67和P53在一定程度上可以客观反映SIL严重程度,联合应用可有效筛选HSIL,提高HSIL诊断准确率,为精准分流SIL病人提供依据,指导个体化治疗.

目的:探讨注射用醋酸亮丙瑞林缓释微球联合屈螺酮炔雌醇片长期维持治疗减缓Ⅲ-Ⅳ期卵巢子宫内膜异位症腹腔镜保守手术后复发的临床疗效,为临床治疗提供依据.方法:选取2016年1月至2018年1月在北京同仁医院妇科行腹腔镜下卵巢子宫内膜异位症保守手术、术中子宫内膜异位症分期(r-AFS,1996年美国生育学会修订)为Ⅲ-Ⅳ期且术后经病理确诊为卵巢子宫内膜异位症、术后门诊按时随访的76例患者为研究对象,采用抽签法将患者随机分为观察组(术后给予注射用醋酸亮丙瑞林缓释微球联合屈螺酮炔雌醇片长期维持治疗)和对照组(术后给予注射用醋酸亮丙瑞林缓释微球),每组38例.对两组患者术前和术后24个月的总主观症状评分进行比较,并比较两组患者复发时间和复发率.结果:观察组患者治疗后的总主观症状评分为(0.94±0.59)分,明显低于对照组的(2.13±0.67)分,差异有统计学意义(P＜0.05).观察组仅有1例患者复发,复发时间为术后34个月,累计复发率为2.6％;对照组5例患者复发,平均复发时间为术后18个月,累计复发率为13.1％;两组患者术后复发率的差异有统计学意义(P＜0.05).结论:Ⅲ-Ⅳ期卵巢子宫内膜异位症腹腔镜保守手术后,应用屈螺酮炔雌醇片长期维持治疗可明显缓解患者临床症状,有效降低远期复发率,延缓疾病复发时间,有一定的临床应用价值.

BACKGROUND:Studies have considered long non-coding RNA 5 prime to Xist (lncRNA FTX) a key lncRNA for normal uterine development, but it has not been reported whether lncRNA FTX is involved in regulating the development of endometriosis (EMs). The aim of the present study was to explore the effect and mechanism of long non-coding RNA 5 prime to Xist (lncRNA FTX) on the invasion, metastasis, and epithelial-mesenchymal transition (EMT) of endometrial stromal cells (ESCs) caused by EMs. METHODS:Ectopic or normal endometrial tissues were collected from 38 patients with EMs, who were diagnosed and operated on at Beijing Tongren Hospital from June 2018 to December 2019, and 20 healthy volunteers with normal endometria. The expression of lncRNA FTX in both groups was detected by quantitative reverse transcription polymerase chain reaction. Ectopic endometrial stromal cells (EESC) and ESC from patients with EMs and healthy volunteers were separated and cultured, and the expression of lncRNA FTX in the cells was detected. The expression of lncRNA FTX in EESC was overexpressed or interfered. Proliferation, invasion, and migration was detected by Cell Counting Kit-8, transwell assay, and scratch assay. Apoptosis and cell cycle were detected by flow cytometry. EMT-related protein and PI3K/Akt signaling pathway-related protein expressions were detected by Western blot. RESULTS:LncRNA FTX was underexpressed in endometrial tissues and EESC from patients with EMs. The overexpression of lncRNA FTX could significantly inhibit the proliferation, invasion, and migration of EESC, but promoted apoptosis and cell cycle arrest in the G0/G1 phase. The overexpression of lncRNA FTX significantly increased the expression of EMT-related protein, E-cadherin, and decreased the protein expression of vimentin, N-cadherin, and zinc finger E-box binding homeobox 1. In addition, the overexpression of lncRNA FTX could decrease the expression of p-PI3K/PI3K and p-Akt/Akt. Interfering with the expression of lncRNA FTX had the opposite result. CONCLUSIONS:The overexpression of lncRNA FTX could decrease the invasion, metastasis, and EMT of ESC caused by EMs by inhibiting the activity of the PI3K/Akt signaling pathway.

目的:探讨湿润烧伤膏对高级别宫颈鳞状上皮内病变应用普通电刀宫颈锥形切除术后伤口转归作用的临床研究.方法:选取2016年1月至2019年1月首都医科大学附属北京同仁医院拟行宫颈锥形切除术患者120例,随机分为观察组和对照组,每组60例.两组患者均于月经期结束3～7 d采用普通电刀行宫颈锥切术,观察组患者宫颈创面电凝止血后应用湿润烧伤膏;对照组患者仅行宫颈电凝止血.观察两组患者术后并发症情况、术后宫颈创面上皮化情况.结果:观察组患者的并发症总发生率为1.7％(1/60),明显低于对照组的15.0％(9/60),差异有统计学意义(P<0.05),且两组患者均无宫颈粘连发生.术后2周、1个月,观察组患者宫颈创面上皮化程度分别为(65.3±11.2)％、(89.4±5.1)％,明显高于对照组的(41.9±6.4)％、(62.5±9.6)％,差异均有统计学意义(P<0.05).结论:湿润烧伤膏应用于普通电刀锥形切除术后创面的并发症少,宫颈愈合效果满意.

目的 探讨加速康复外科(enhanced recovery after surgery,ERAS)在腹腔镜卵巢良性肿瘤手术中应用的安全性及有效性.方法 选取2016年2月至2018年2月在北京同仁医院妇科行卵巢良性肿瘤手术的患者共120例,其中加速康复组60例,传统妇科组60例,根据围手术期处理方式将患者分为加速康复组和传统妇科组,比较两组患者的一般情况、手术方式、术后首次排气时间、术后住院时间.术后不同时间点采用视觉模拟评分(VAS)评定患者术后疼痛程度.结果 加速康复组与传统妇科组术后首次排气时间分别为(18.0±6.2)、(50.0±10.7)h,术后住院时间分别为(2.2±1.0)、(4.2±1.1)天,差异有统计学意义(均P<0.05).但两组的手术时间、术中出血量差别无统计学意义(均P>0.05).加速康复组术后不同时间疼痛评分与传统妇科组比较差异有统计学意义(P<0.05).结论 加速康复治疗的患者较传统妇科治疗患者的术后胃肠功能恢复快、痛苦小、术后住院时间短,患者住院费用也相应降低,并且不增加手术并发症,值得临床推广.

Increased endometrial stromal cell (ESC) survival and migration is responsible for the development and progression of endometriosis. However, little is known about the mechanisms underlying ESC survival and migration, and limited therapeutic strategies that are able to reverse these abnormalities are available. The present study investigated the effects of zearalenone (ZEA) on ESC survival and migration, particularly focusing on mitochondrial fission and the c-Jun N-terminal kinase (JNK)/dynamin-related protein 1 (Drp1) pathway. The results revealed that ZEA induced ESC apoptosis in a dose-dependent manner. Furthermore, ZEA treatment triggered excessive mitochondrial fission resulting in structural and functional mitochondrial damage, leading to the collapse of the mitochondrial membrane potential and subsequent leakage of cytochrome c into the cytoplasm. This triggered the mitochondrial pathway of apoptosis. Additionally, ZEA‐induced mitochondrial fission decreased ESC migration through F-actin/G-actin homeostasis dysregulation. ZEA also increased JNK phosphorylation and subsequently Drp1 phosphorylation at the serine 616 position, resulting in Drp1 activation. JNK/Drp1 pathway inhibition abolished the inhibitory effects of ZEA on ESC survival and migration. In summary, the present study demonstrated that ZEA reduced ESC survival and migration through the stimulation of mitochondrial fission by activation of the JNK/Drp1 pathway.

Increased endometrial stromal cell (ESC) survival and migration is responsible for the development and progression of endometriosis. However, little is known about the mechanisms underlying ESC survival and migration, and limited therapeutic strategies that are able to reverse these abnormalities are available. The present study investigated the effects of zearalenone (ZEA) on ESC survival and migration, particularly focusing on mitochondrial fission and the c‑Jun N‑terminal kinase (JNK)/dynamin‑related protein 1 (Drp1) pathway. The results revealed that ZEA induced ESC apoptosis in a dose‑dependent manner. Furthermore, ZEA treatment triggered excessive mitochondrial fission resulting in structural and functional mitochondrial damage, leading to the collapse of the mitochondrial membrane potential and subsequent leakage of cytochrome c into the cytoplasm. This triggered the mitochondrial pathway of apoptosis. Additionally, ZEA‑induced mitochondrial fission decreased ESC migration through F‑actin/G‑actin homeostasis dysregulation. ZEA also increased JNK phosphorylation and subsequently Drp1 phosphorylation at the serine 616 position, resulting in Drp1 activation. JNK/Drp1 pathway inhibition abolished the inhibitory effects of ZEA on ESC survival and migration. In summary, the present study demonstrated that ZEA reduced ESC survival and migration through the stimulation of mitochondrial fission by activation of the JNK/Drp1 pathway.

With regard to teaching of gynecology, the integrated medical pattern should be built up and applied, which could help culturing students' ability of active thinking, analyzing and solving clinical problems. To set up the concept of individual-based treatment, we applied the concept of holistic integrated medicine in clinical teaching of gynecology, combing the aspects of teaching, clinic and resources. A reform in teaching content design and teaching mode had been carried out. The results showed that the students are highly satisfied with teaching process, their enthusiasm of learning has been effectively boosted, their clini-cal thinking has been initially cultivated. In a word, this teaching reform has achieved good results in practice.

Objective To evaluate the effects of PBL bilingual teaching method in the clinical practice of Obstetrics and Gynecology. Methods Experimental class students of 2010 and 2011 grade were divided into two groups at random: Traditional bilingual group and PBL bilingual group, teaching efficiency was assessed by using theory test and questionnaire. Results There is no significant difference in objective item examinational score(>0.05), but There is significant difference in subjective item examinational score(P<0.01). The questionnaire show that 70 percent of students think PBL bilingual teaching has better effects on improving the abilities of specialized vocabulary using, and consulting foreign data by using exact keywords. 85 percent of students think PBL bilingual teaching also has better effects on improving the abilities of spoken English communication. 75 percent of students think that PBL bilingual teaching also can improve the team cooperation spirit. Conclusions PBL bilingual teaching is one of the good modes in the cultivation of talents.

目的 探讨卡前列甲酯栓(卡孕栓)在高危妊娠剖宫产产妇中的应用价值及其对胃肠功能状态的影响.方法 选择2014年4月～2015年4月于首都医科大学附属北京同仁医院行剖宫产术的90例高危妊娠产妇,将其随机分为对照组(缩宫素)和联合用药组(缩宫素联合卡孕栓),每组各45例.观察比较两组产妇产后10、20、30 min的宫缩强度、产前及产后6、12、24、48 h的血小板活化指标(CD41a、CD63、GPⅡb/Ⅲa、GMP-140)及胃肠激素(胃泌素、胃动素、血管活性肠肽及神经肽Y)水平.结果 产后10、20、30 min联合用药组的宫缩强度均大于对照组,产后6、12、24h的血小板活化指标低于对照组,但高于本组产前,差异均有统计学意义(均P＜0.05);产后6、12、24、48 h联合用药组胃肠激素血清表达水平高于对照组,但低于本组产前,差异均有统计学意义(均P＜0.05).结论 卡孕栓在高危妊娠剖宫产产妇中的应用价值较高,对于机体多方面的影响更为积极,适用于高危妊娠剖宫产产妇.

To carry out bilingual education among medical students in clinical practice is for further meeting the need of cultivating international students .And also is the trend of present educational re-form .In this paper ,the combination of obstetrics and gynecology knowledge ,bilingual teaching and clinical practice pattern during ward-round has greatly enhanced the level of medical English in students and promoted their study activity .The application of bilingual teaching in the internal clinical practice will improve clinical practice quality and comprehensive practice quality of medical students .

目的:探讨绝经后子宫出血的原因并评价宫腔镜对绝经后子宫出血的诊断价值.方法:回顾性分析2010年6月～2012年6月198例绝经后异常子宫出血患者的宫腔镜结合病理组织学检查结果.结果:198例宫腔镜诊断与病理组织学检查的符合率为87.4％,其中良性病变和非器质性病变132例,恶性病变仅18例,良性病变中以子宫内膜息肉最多.结论:宫腔镜检查结合病理组织学检查可以发现绝经后子宫出血的原因,尽早确诊子宫内膜癌和癌前病变.

The clinical practice is an important link of medical education.Students can consolidate theory knowledge through the clinical practice.many kinds of teaching method including teacher simulation standardized patients teaching method,problem based teaching method,evidence-based medicine teaching method, multimedia teaching method were used to improve the teaching quality of gynecology and obstetrics.Teachers should stimulate students' interest in study,mobilize subjective initiative of learning,cultivate ability of find,analysis and problem solving to achieve good teaching effect.

Objective To investigate the differential expression of 14-3-3 proteins in platinum resistance and platinum sensitive ovarian cancer cell clones.Methods The proteins of C0C1 and C0C1/DDP were separated by highly sensitive two-dimensional differential gel electrophoresis(2-D DIGE) coupled with Matrix-assisted laser desorption/ionization-time of flight(MALDI-TOF-MS) for the identification of protein differentially expressed in cisplatin sensitive and cisplatin resistance cell lines.Results We found a group of the distinct expressed proteins between C0C1 and C0C1/DDP.Among them,and 14-3-3ζwere the up regulated proteins(14-3-3σ up regulated 31%.Student′s t-test;P=0.0049;14-3-3ζ up regulated 38%.Student′s t-test;P=0.0032) .Conclusions The differential expression of 14-3-3proteins in C0C1/DDP lays foundation for the research of ovarian cancer platinum resistance,and offers new therapy targets for them.

OBJECTIVE:To study the expression of NASP in platinum resistance ovarian cancer cell clones (C0C1/DDP).METHODS:The proteins of C0C1 and C0C1/DDP were separated by highly sensitive two-dimensional differential gel electrophoresis (2-D DIGE) coupled with matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF-MS) to identify the proteins differentially expressed in cisplatin sensitive and cisplatin resistance cell lines.RESULTS:The investigators found a group of the distinct expressed proteins between C0C1 and C0C1/DDP. Among them, NASP was one of the down-regulated proteins by 21% (Student's t-test; P = 0.01).CONCLUSION:The identification of NASP in C0C1/DDP has laid a foundation for future in-depth researches into the mechanisms of ovarian cancer platinum resistance.