After canine breast tumor cell line CHMm was treated with HMME-PDT.Growth curve of cells in each group was drawn after trypan-blue staining.The changes in the levels of IL-6 and IL-2 were detected with radioimmunoassay method after PDT at different times.The cell death rate of PDT group with trypan-blue staining increased more obviously compared with controls.The levels of IL-2 increased but IL-6 decreased after PDT as time continued.The lethal effect of PDT on canine breast tumor cell line CHMm might be caused by the change in the levels of IL-6 and IL-2 in these cells.

20多年前,人们根据多种肿瘤动力学研究中出现的细胞自然丢失现象,提出了细胞凋亡(apoptosis,AP)理论[1],近年来,细胞凋亡在医学细胞生物学界倍受关注,成为研究的热点之一.引起细胞凋亡的因素很多,现在研究发现激光光动力学作用也可以诱导细胞凋亡[2],并认为其可能是低强度激光照射产生临床治疗作用的基础.因此本文拟从He-Ne激光光动力学诱导细胞凋亡这一角度做一概述和探讨.

紫杉醇是一种新型的抗癌药物,它作用于细胞的微管,抑制其解聚,从而抑制纺锤丝功能,使细胞有丝分裂停滞[1],因此紫杉醇具有抑制癌细胞生长的功能.Dziadyk J M等用脉冲式给药法研究紫杉醇诱导细胞凋亡的作用,结果发现给药后细胞分裂停滞迅速发生[2],现在紫杉醇已经成功应用于人乳腺癌的临床治疗上,但是将紫杉醇用于犬乳腺肿瘤的治疗报道几乎没有.

To provide external haemostat for animal,prothrombin,Fxa,FV,phosphate lipid and Ca2+ were used to make thrombin through orthogonal experiment designment and eventually thrombin was made into external haemostat according to demanding of veterinary clinic.We can draw the conclusion that the external haemostat made in this test is fit for animal external use in clinic,and is much more advance than other coagulant.