The development level of agricultural industrialization in different counties(cities),Baoding city was quantitatively analyzed by data collection and the factor analysis method based on building the operability evaluation index system to provide a reference for policy formulation in agricultural industrialization.The results showed that there was the significant gap in agricultural industrialization between different counties(cities)and the distribution was not balance in Baoding city.The development of agricultural industrialization in poor mountain areas was very slow.The suggestions to increase agricultural input intensity,rationally adjust agricultural structure,actively cultivate agricultural scale operation proprietors and implement poverty alleviation policies in agricultural industrialization were proposed in the paper at the same time.

为研究平原地区土地利用效益，以山东省潍坊市为例，在构建具有可操作性评价指标体系的基础上，通过资料收集与整理，运用因子分析法，对潍坊市的土地利用效益进行定量分析，结果表明，寿光市得分最高，为0．956，奎文区得分最低，为-0．538，潍坊各县（市、区）土地利用综合效益存在明显差异。在此基础上，分析造成潍坊市各县（市、区）土地利用效益差异的原因，在借鉴土地利用富有效率地区成功经验的基础上，针对性地提出改善、提高平原地区土地利用效益的对策建议。

农家书屋工程是社会主义新农村文化建设的重要内容之一，河北省在农家书屋建设过程中取得了一些成绩，同时也存在一些问题，本文针对这些问题提出了农家书屋建设的对策建议。

农户在种植过程中对化肥、农药、农膜和农用机械的依赖程度较高，严重影响了低碳农业的发展。本文经过调查分析，发现受教育水平和地区经济发展水平对农户低碳化种植的认知和意愿有显著影响，同时低碳技术、种植成本、政府政策、传统种植偏好是影响农户低碳化种植的主要因素。

为了研究贫困山区农业产业化发展状况,以河北省涞源县为例,分析了农业产业化经营现状以及存在的主要问题,并提出发展对策,以为涞源县农业产业化经营提供指导。

【目的】研究拟南芥MAPK信号转导途径的关键基因MPK3、MPK4、MPK6的功能,明确其在渗透调节中的作用。【方法】构建拟南芥MPK3、MPK4、MPK6的酿酒酵母表达载体,遗传转化酿酒酵母渗透调节功能丧失的hog1?突变体,筛选得到阳性转化子,并分析其在渗透胁迫下的表型特征。【结果】扩增得到了拟南芥MPK3、MPK4、MPK6的全长cDNA序列,构建了上述3个基因的表达载体,并筛选得到了3个基因的阳性转化子。在1 mol.L-1 KCl、0.3 mol.L-1 LiCl、1 mol.L-1 NaCl、1 mol.L-1 Sorbitol的盐胁迫处理下,阳性转化子生长状态良好,与野生型的表型基本一致,均恢复了hog1?对盐胁迫的抗性。在盐胁迫处理下,hog1?细胞形态异常且体内甘油含量较野生型低,而转化子的形态和体内甘油含量均恢复到正常的表型。【结论】MPK3、MPK4、MPK6均能够使酿酒酵母渗透调节功能丧失突变体hog1?恢复对盐胁迫的抗性,具有渗透调节的功能。

Objective) The objective of this study is to identify the structure characteristics, transcription activity and function of StSte12 from Setosphaeria turcica. (Method) Conservative domain prediction and phylogenetic tree analysis were used to predict the possible function of StSte12 through bioinformatics analysis. The transcription activity of StSTE12 was tested by β-galactosidase activity method. Through characterization of growth of the StSTE12 complement transformant, in which StSTE12 was introduced into ScSTE12 null mutant of Saccharomyces ceresivisiae, the function of StSTE12 was preliminarily identified.(Result) StSte12 contained STE homeodomain and ZnF_C2H2 structure, which was the characteristics of Ste12-like transcription factor. Sequence analysis revealed that StSTE12 shared highly homology with other STE12-like genes of plant pathogens. StSte12 had transcription activation in vitro and could restore the function of ste12Δ of S. ceresivisiae, which regulated invasion growth of the yeast cell. (Conclusion) Transcription factor StSTE12, a STE12-like gene from S. turcica, has a transcription activity and plays an important role in regulating the invasion growth of the cell on YPD medium.

【Objective】The objective of this study is to measure the effect of hyperosmotic stress on the growth and development of Setosphaeria turcica,to analyze whether or not the glycerol is one of the compatible solutes,and to detect the expression characteristic of STK1 when the pathogen is cultured under hyperosmotic stress.【Method】The growth and development of S.turcica,which cultured under 2 different hyperosmotic stresses,were observed.By analyzing the change of glycerol content in mycelium cells,it was confirmed that glycerol was one of the compatible solutes.The expression characteristic of STK1 was detected using semiquantitative RT-PCR method.【Result】Isotonic solution concentration of hyphal cells of S.turcica was 0.78 mol.L-1.The colony color changed distinctly under hyperosmotic stress.The colony cultured on PDA medium containing 1 mol.L-1 NaCl was reddish-brown in colour,the growth rate of the colony was repressed remarkably,cultured on PDA medium containing 1 mol.L-1 NaCl showed strong inhibition.The condensation was observed in the protoplasts when cultured in 1 mol.L-1 NaCl treatment.With the increase of hyperosmotic stress time and compatible solute concentration,glycerol content increased evidently.The expression level of STK1 enhanced distinctly within 48 hours under hyperosmotic stress【.Conclusion】Colony growth rate was inhibited,colony color turned obviously,protoplast became concentrated,some mycelium cells enlarged and changed into globosed under hyperosmotic stress in S.turcica.Glycerol was one of the compatible solutes in the mycelium cells of S.turcica.STK1 took part in regulating hyperosmotic stress responses in S.turcica.

【Objective】 The objective of this study is to use Agrobacterium tumefaciens-mediated transformation(ATMT) to mutate the genes of Setosphaeria turcica and construct ATMT mutant library,which will lay a foundation for illuminating the pathogenesis of S.turcica at molecular level.【Method】A.tumefacien with binary recombination vector was transformed into S.turcica,and the mutants were screened by hygromycin B and PCR technology.Based on the methods,a preliminary ATMT mutant library was constructed.Some mutants were selected randomly and analysed about the colony morphologies,mycelium and conidium development,and pathogenicity.【Result】A total of 1 265 T-DNA insertion mutants were obtained in this experiment.Hygromycin B resistance screening and PCR technology were used to confirm 36 mutants,which were selected randomly from the mutant library.The results showed that hygromycin phosphotransferase genes had been integrated into wild-type genome of S.turcica and the mutants were stable in their characteristics of genetics.Compared with wild-type strains,most of the mutants did not changed their colony morphologies and growth rates,only minorities of the mutants varied in their characteristics,in which 13.8% strains slowed down their growth rate obviously,16.7% strains accelerated their growth rate distinctly,about 5.6% strains altered their shapes of conidia,13.5% strains reduced the number of conidia,16.6% strains changed their germination rate evidently,and 1 strain reinforced its pathogenicity,accounting 2.8% in all selected strains.【Conclusion】 ATMT mutant library of S.turcica was constructed and some mutants were analysed primarily.The study will lay a foundation for cloning genes related to growth,development and pathogenicity of S.turcica in the future.